Restriction map for
pFN21AB4120
(Circular) (Six-base) MAPSORT of: pFN21AB4120.seq Check: 392 from: 1 to: 6058
pFN21AB4120 6058 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2392 2392
Size: 6058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4121 5505 5773 1632
Size: 2489 1384 268 1917
Fragments arranged by size:
2489 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2515 2547 2515
Size: 32 6026
AlwNI CAG_nnn'CTG
Cuts at: 2080 2230 2840 3801 5373 2080
Size: 150 610 961 1572 2765
Fragments arranged by size:
2765 1572 961 610 150
ApaI G_GGCC'C
Cuts at: 3163 3163
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 2992 3367 3593 3745 5854 1493
Size: 1499 375 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 375 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2827 3383 1870
Size: 73 142 742 556 4545
Fragments arranged by size:
4545 742 556 142 73
BamHI G'GATC_C
Cuts at: 3148 3388 3755 3148
Size: 240 367 5451
Fragments arranged by size:
5451 367 240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2834 3163 3377 1391
Size: 1443 329 214 4072
Fragments arranged by size:
4072 1443 329 214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 928
Size: 971 360 4727
Fragments arranged by size:
4727 971 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2858 2892 4249 4283 1903
Size: 34 921 34 1357 34 3678
Fragments arranged by size:
3678 1357 921 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2909 3950 5160 2909
Size: 1041 1210 3807
Fragments arranged by size:
3807 1210 1041
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BfrBI ATG'CAT
Cuts at: 2204 2204
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 2833 2955 3475 2833
Size: 122 520 5416
Fragments arranged by size:
5416 520 122
Bme1580I G_kGCm'C
Cuts at: 1790 3163 4119 5275 1790
Size: 1373 956 1156 2573
Fragments arranged by size:
2573 1373 1156 956
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2588 2842 3789
Size: 416 53 83 399 24 1171 254 947
Cuts at: 3789 4747 442
Size: 958 1753
Fragments arranged by size:
1753 1171 958 947 416 399 254 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 4718 1239
Size: 968 2511 2579
Fragments arranged by size:
2579 2511 968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 2701 4721 882
Size: 617 645 557 2020 2219
Fragments arranged by size:
2219 2020 645 617 557
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 3147 6052 3147
Size: 2905 3153
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2515 2545 1178
Size: 30 165 30 624 30 458 30 4691
Fragments arranged by size:
4691 624 458 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2048 2162 3119 1186
Size: 859 3 114 957 4125
Fragments arranged by size:
4125 957 859 114 3
BseYI C'CCAG_C
Cuts at: 5261 5757 5261
Size: 496 5562
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4119 4204 5275 4119
Size: 85 1071 4902
Fragments arranged by size:
4902 1071 85
BsmI GAATG_Cn'
Cuts at: 2778 3569 3662 2778
Size: 791 93 5174
Fragments arranged by size:
5174 791 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2834 3163 3377 4119 4204 5275 1391
Size: 399 1044 329 214 742 85 1071 2174
Fragments arranged by size:
2174 1071 1044 742 399 329 214 85
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2392 3413 844
Size: 1548 1021 3489
Fragments arranged by size:
3489 1548 1021
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2784 4553 4727 65
Size: 1183 1536 1769 174 1396
Fragments arranged by size:
1769 1536 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 2037 3148 3388 3755 4140 4157 5609 5695
Size: 65 1111 240 367 385 17 1452 86
Cuts at: 5695 5707 6053 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1111 385 367 346 240 86
65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2455 513
Size: 552 939 451 4116
Fragments arranged by size:
4116 939 552 451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 3574 4368 4388 3574
Size: 794 20 5244
Fragments arranged by size:
5244 794 20
ClaI AT'CG_AT
Cuts at: 1554 2427 1554
Size: 873 5185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 2700 3362 2700
Size: 662 5396
DraIII CAC_nnn'GTG
Cuts at: 2723 2723
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3505 4394
Size: 54 1425 50 275 18 378 1297 889
Cuts at: 4394 6023 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1297 889 378 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2317 3988 1353
Size: 964 1671 3423
Fragments arranged by size:
3423 1671 964
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 4121 4718 5505 5773 1239
Size: 393 575 1914 597 787 268 1524
Fragments arranged by size:
1914 1524 787 597 575 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3160 3160
Size: 6058
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
EcoRV GAT'ATC
Cuts at: 2236 2874 2236
Size: 638 5420
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2060 2401 5205 1678
Size: 382 341 2804 2531
Fragments arranged by size:
2804 2531 382 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2512 2544 3212
Size: 32 135 32 455 32 453 32 668
Cuts at: 3212 3244 4699 4731 4773 4805 1373
Size: 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1455 668 455 453 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2139 748
Size: 1391 4667
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 2997 3657 2997
Size: 660 5398
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3035 5172 5356 1305
Size: 1730 2137 184 2007
Fragments arranged by size:
2137 2007 1730 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 10
Size: 54 1425 343 378 3858
Fragments arranged by size:
3858 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 2967 3047 4016 4375 4534 518
Size: 648 1623 178 80 969 359 159 2042
Fragments arranged by size:
2042 1623 969 648 359 178 159 80
MspA1I CmG'CkG
Cuts at: 1606 3474 4151 5299 5544 1606
Size: 1868 677 1148 245 2120
Fragments arranged by size:
2120 1868 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2455 513
Size: 552 939 451 4116
Fragments arranged by size:
4116 939 552 451
NdeI CA'TA_TG
Cuts at: 387 3136 387
Size: 2749 3309
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NsiI A_TGCA'T
Cuts at: 2206 2206
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3416 4961 1132
Size: 97 2187 1545 2229
Fragments arranged by size:
2229 2187 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3320 1348
Size: 1972 4086
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2230 1108
Size: 1122 4936
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2547 4127 4159 5640 5672 2515
Size: 32 1580 32 1481 32 2901
Fragments arranged by size:
2901 1580 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 2598 3260 3628 2598
Size: 662 368 5028
Fragments arranged by size:
5028 662 368
PspOMI G'GGCC_C
Cuts at: 3159 3159
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2019 3115 3410 830
Size: 1189 1096 295 3478
Fragments arranged by size:
3478 1189 1096 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2015 2124 3111 3406 4541 5222 5413
Size: 219 970 109 987 295 1135 681 191
Cuts at: 5413 826
Size: 1471
Fragments arranged by size:
1471 1135 987 970 681 295 219 191
109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2829 3385 1872
Size: 957 556 4545
Fragments arranged by size:
4545 957 556
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4162 5063 5325 820
Size: 197 926 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2004 2455 2711 3497 513
Size: 552 939 451 256 786 3074
Fragments arranged by size:
3074 939 786 552 451 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2824 4085 4102 4255 4440 4885 325
Size: 1255 1244 1261 17 153 185 445 1498
Fragments arranged by size:
1498 1261 1255 1244 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2218 4304 6016
Size: 274 80 33 51 1443 241 2086 1712
Cuts at: 6016 96
Size: 138
Fragments arranged by size:
2086 1712 1443 274 241 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2707 2739 6018 6050 2707
Size: 32 3279 32 2715
Fragments arranged by size:
3279 2715 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 2827 3383 1870
Size: 957 556 4545
Fragments arranged by size:
4545 957 556
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI