Restriction map for
pFN21AB4115
(Circular) (Six-base) MAPSORT of: pFN21AB4115.seq Check: 9903 from: 1 to: 7519
pFN21AB4115 7519 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5329 278
Size: 53 83 186 936 3793 2468
Fragments arranged by size:
3793 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3160 4862 1937
Size: 1223 1702 4594
Fragments arranged by size:
4594 1702 1223
Acc65I G'GTAC_C
Cuts at: 4840 4840
Size: 7519
AclI AA'CG_TT
Cuts at: 1563 2259 5646 6019 1563
Size: 696 3387 373 3063
Fragments arranged by size:
3387 3063 696 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 3107 3480 3558 4362 5582 6966 7234
Size: 756 719 373 78 804 1220 1384 268
Cuts at: 7234 1632
Size: 1917
Fragments arranged by size:
1917 1384 1220 804 756 719 373 268
78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7322
AflIII A'CryG_T
Cuts at: 1203 3871 6418 7395 1203
Size: 2668 2547 977 1327
Fragments arranged by size:
2668 2547 1327 977
AgeI A'CCGG_T
Cuts at: 6350 6350
Size: 7519
AhdI GACnn_n'nnGTC
Cuts at: 6248 6248
Size: 7519
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7519
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3048 1184
Size: 1864 5655
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 2079
Size: 32 7487
AlwNI CAG_nnn'CTG
Cuts at: 5262 6834 5262
Size: 1572 5947
ApaLI G'TGCA_C
Cuts at: 5576 6732 5576
Size: 1156 6363
ApoI r'AATT_y
Cuts at: 1493 3485 4216 4828 5054 5206 7315 1493
Size: 1992 731 612 226 152 2109 1697
Fragments arranged by size:
2109 1992 1697 731 612 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6073 160
Size: 872 5041 1606
Fragments arranged by size:
5041 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7519
AvaI C'yCGr_G
Cuts at: 1870 1943 4844 1870
Size: 73 2901 4545
Fragments arranged by size:
4545 2901 73
AvrII C'CTAG_G
Cuts at: 3976 3976
Size: 7519
BamHI G'GATC_C
Cuts at: 4849 5216 4849
Size: 367 7152
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4325 4840 6295 618
Size: 325 217 627 2538 515 1455 1842
Fragments arranged by size:
2538 1842 1455 627 515 325 217
BanII G_rGCy'C
Cuts at: 1391 4007 4357 4698 4838 1391
Size: 2616 350 341 140 4072
Fragments arranged by size:
4072 2616 350 341 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 928
Size: 971 645 5903
Fragments arranged by size:
5903 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2789 2823 5710 5744 1903
Size: 34 852 34 2887 34 3678
Fragments arranged by size:
3678 2887 852 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4526 5411 6621 4526
Size: 885 1210 5424
Fragments arranged by size:
5424 1210 885
BclI T'GATC_A
Cuts at: 1548 2614 1548
Size: 1066 6453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 6130 136
Size: 107 122 71 631 605 451 4007 1525
Fragments arranged by size:
4007 1525 631 605 451 122 107 71
BglII A'GATC_T
Cuts at: 2704 3892 7514 2704
Size: 1188 3622 2709
Fragments arranged by size:
3622 2709 1188
BlpI GC'TnA_GC
Cuts at: 4936 4936
Size: 7519
Bme1580I G_kGCm'C
Cuts at: 1790 4330 5580 6736 1790
Size: 2540 1250 1156 2573
Fragments arranged by size:
2573 2540 1250 1156
BmgBI CAC'GTC
Cuts at: 1318 4502 1318
Size: 3184 4335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3647 4413 5250
Size: 416 53 83 399 24 2230 766 837
Cuts at: 5250 6208 442
Size: 958 1753
Fragments arranged by size:
2230 1753 958 837 766 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3548 1056
Size: 2492 5027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6179 1239
Size: 4940 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5644 6509 6807 5644
Size: 865 298 6356
Fragments arranged by size:
6356 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2737 6182 882
Size: 617 1238 3445 2219
Fragments arranged by size:
3445 2219 1238 617
BsaAI yAC'GTr
Cuts at: 493 1204 5310 493
Size: 711 4106 2702
Fragments arranged by size:
4106 2702 711
BsaBI GATnn'nnATC
Cuts at: 2343 3600 4572 7513 2343
Size: 1257 972 2941 2349
Fragments arranged by size:
2941 2349 1257 972
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 5326 5708 6343
Size: 53 83 186 936 1942 1851 382 635
Cuts at: 6343 275
Size: 1451
Fragments arranged by size:
1942 1851 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3846 5952 6350 6624 6771 7182 3846
Size: 2106 398 274 147 411 4183
Fragments arranged by size:
4183 2106 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2079 2109 1178
Size: 30 165 30 676 30 6588
Fragments arranged by size:
6588 676 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3105 3500 3984 1186
Size: 1919 395 484 4721
Fragments arranged by size:
4721 1919 484 395
BseYI C'CCAG_C
Cuts at: 2072 4181 6722 7218 2072
Size: 2109 2541 496 2373
Fragments arranged by size:
2541 2373 2109 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2727 3404 4224 1163
Size: 573 991 677 820 4458
Fragments arranged by size:
4458 991 820 677 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4969 5730 6758 1540
Size: 275 186 2968 761 1028 2301
Fragments arranged by size:
2968 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5580 5665 6736 5580
Size: 85 1071 6363
Fragments arranged by size:
6363 1071 85
BsmI GAATG_Cn'
Cuts at: 2357 3913 5030 5123 2357
Size: 1556 1117 93 4753
Fragments arranged by size:
4753 1556 1117 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4007 4330 4357 4698 4838 5580 5665
Size: 399 2217 323 27 341 140 742 85
Cuts at: 5665 6736 1391
Size: 1071 2174
Fragments arranged by size:
2217 2174 1071 742 399 341 323 140
85 27
BspEI T'CCGG_A
Cuts at: 3846 7182 3846
Size: 3336 4183
BspHI T'CATG_A
Cuts at: 5408 7138 5408
Size: 1730 5789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4874 844
Size: 4030 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3779 3991 5406 1394
Size: 429 1956 212 1415 3507
Fragments arranged by size:
3507 1956 1415 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 3906 4104 6014 6188 65
Size: 1183 2658 198 1910 174 1396
Fragments arranged by size:
2658 1910 1396 1183 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4611 6163 6350 1700
Size: 110 2801 1552 187 2869
Fragments arranged by size:
2869 2801 1552 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7519
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7519
BssSI C'ACGA_G
Cuts at: 3732 5579 6591 3732
Size: 1847 1012 4660
Fragments arranged by size:
4660 1847 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7519
BstBI TT'CG_AA
Cuts at: 6323 6323
Size: 7519
BstEII G'GTnAC_C
Cuts at: 1184 3836 1184
Size: 2652 4867
BstXI CCAn_nnnn'nTGG
Cuts at: 4529 4529
Size: 7519
BstYI r'GATC_y
Cuts at: 1972 2192 2704 3027 3892 4849 5216 5601 5618
Size: 220 512 323 865 957 367 385 17
Cuts at: 5618 7070 7156 7168 7514 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 957 865 512 385 367 346
323 220 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3589 3682 4522 513
Size: 552 2524 93 840 3510
Fragments arranged by size:
3510 2524 840 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2352 487
Size: 683 56 1126 5654
Fragments arranged by size:
5654 1126 683 56
BtsI GCAGTG_nn'
Cuts at: 2997 5035 5829 5849 2997
Size: 2038 794 20 4667
Fragments arranged by size:
4667 2038 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3655 3690 4303 4338 529
Size: 35 3091 35 613 35 3710
Fragments arranged by size:
3710 3091 613 35 35 35
DraI TTT'AAA
Cuts at: 4823 4823
Size: 7519
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3653 4268 4342 6526 809
Size: 507 2337 615 74 2184 1802
Fragments arranged by size:
2337 2184 1802 615 507 74
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3078 3348 3663
Size: 54 1425 50 275 18 1248 270 315
Cuts at: 3663 3763 4570 4966 5855 7484 8
Size: 100 807 396 889 1629 43
Fragments arranged by size:
1629 1425 1248 889 807 396 315 275
270 100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4966 1537
Size: 275 3154 4090
Fragments arranged by size:
4090 3154 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2140 2393 2454 2526 2607 2664 3499 3534
Size: 787 253 61 72 81 57 835 35
Cuts at: 3534 3769 5449 1353
Size: 235 1680 3423
Fragments arranged by size:
3423 1680 835 787 253 235 81 72
61 57 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6106 6480 6626 387
Size: 1064 138 4517 374 146 1280
Fragments arranged by size:
4517 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 3107 3480 3558 4362 5582 6179
Size: 393 756 719 373 78 804 1220 597
Cuts at: 6179 6966 7234 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1220 804 787 756 719 597 393
373 268 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2754 3125 3560 4068 2754
Size: 371 435 508 6205
Fragments arranged by size:
6205 508 435 371
EcoO109I rG'GnC_Cy
Cuts at: 3118 3118
Size: 7519
EcoRI G'AATT_C
Cuts at: 4216 4828 5206 4216
Size: 612 378 6529
Fragments arranged by size:
6529 612 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3225 4150 3225
Size: 925 6594
FspI TGC'GCA
Cuts at: 6025 6025
Size: 7519
HaeII r_GCGC'y
Cuts at: 1678 6666 1678
Size: 4988 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2617 2649 2986 3018 4465
Size: 32 135 32 1045 32 337 32 1447
Cuts at: 4465 4497 6160 6192 6234 6266 1373
Size: 32 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 1447 1045 337 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3161 4366 4863 5109 669
Size: 988 281 1223 1205 497 246 3079
Fragments arranged by size:
3079 1223 1205 988 497 281 246
HindIII A'AGCT_T
Cuts at: 748 2478 4668 748
Size: 1730 2190 3599
Fragments arranged by size:
3599 2190 1730
HpaI GTT'AAC
Cuts at: 5109 5109
Size: 7519
KpnI G_GTAC'C
Cuts at: 4844 4844
Size: 7519
MfeI C'AATT_G
Cuts at: 2571 2985 5118 2571
Size: 414 2133 4972
Fragments arranged by size:
4972 2133 414
MluI A'CGCG_T
Cuts at: 7395 7395
Size: 7519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2946 4250 4625 6633 6817 1305
Size: 1641 1304 375 2008 184 2007
Fragments arranged by size:
2008 2007 1641 1304 375 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3350 3665 4572 10
Size: 54 1425 343 1518 315 907 2957
Fragments arranged by size:
2957 1518 1425 907 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4509 4527 4566 5477 5836 5995 518
Size: 648 3343 18 39 911 359 159 2042
Fragments arranged by size:
3343 2042 911 648 359 159 39 18
MspA1I CmG'CkG
Cuts at: 1606 4763 4935 5612 6760 7005 1606
Size: 3157 172 677 1148 245 2120
Fragments arranged by size:
3157 2120 1148 677 245 172
NaeI GCC'GGC
Cuts at: 4613 4613
Size: 7519
NcoI C'CATG_G
Cuts at: 513 1065 3589 4522 513
Size: 552 2524 933 3510
Fragments arranged by size:
3510 2524 933 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7519
NgoMIV G'CCGG_C
Cuts at: 4611 4611
Size: 7519
NheI G'CTAG_C
Cuts at: 1052 3544 1052
Size: 2492 5027
NotI GC'GGCC_GC
Cuts at: 4966 4966
Size: 7519
NspI r_CATG'y
Cuts at: 1132 1229 4877 6422 1132
Size: 97 3648 1545 2229
Fragments arranged by size:
3648 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7519
PciI A'CATG_T
Cuts at: 6418 6418
Size: 7519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2513 2978 4301 1108
Size: 902 503 465 1323 4326
Fragments arranged by size:
4326 1323 902 503 465
PmeI GTTT'AAAC
Cuts at: 4823 4823
Size: 7519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2111 5588 5620 7101 7133 2079
Size: 32 3477 32 1481 32 2465
Fragments arranged by size:
3477 2465 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7519
PsiI TTA'TAA
Cuts at: 5089 5089
Size: 7519
PstI C_TGCA'G
Cuts at: 830 2920 4871 830
Size: 2090 1951 3478
Fragments arranged by size:
3478 2090 1951
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7519
PvuII CAG'CTG
Cuts at: 4763 4763
Size: 7519
SalI G'TCGA_C
Cuts at: 1936 3159 4861 1936
Size: 1223 1702 4594
Fragments arranged by size:
4594 1702 1223
SapI GCTCTTCn'nnn_
Cuts at: 2140 3534 3769 2140
Size: 1394 235 5890
Fragments arranged by size:
5890 1394 235
SbfI CC_TGCA'GG
Cuts at: 4871 4871
Size: 7519
ScaI AGT'ACT
Cuts at: 5767 7479 5767
Size: 1712 5807
SexAI A'CCwGG_T
Cuts at: 2971 3840 2971
Size: 869 6650
SfcI C'TryA_G
Cuts at: 826 1045 2916 3982 4867 6002 6683 6874 826
Size: 219 1871 1066 885 1135 681 191 1471
Fragments arranged by size:
1871 1471 1135 1066 885 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7519
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7519
SmaI CCC'GGG
Cuts at: 1872 4846 1872
Size: 2974 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5623 6524 6786 820
Size: 197 926 3680 901 262 1553
Fragments arranged by size:
3680 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5310 493
Size: 4817 2702
SpeI A'CTAG_T
Cuts at: 152 4199 152
Size: 4047 3472
SphI G_CATG'C
Cuts at: 1132 4877 1132
Size: 3745 3774
SspI AAT'ATT
Cuts at: 5 52 5443 5
Size: 47 5391 2081
Fragments arranged by size:
5391 2081 47
StuI AGG'CCT
Cuts at: 1517 3975 4055 1517
Size: 2458 80 4981
Fragments arranged by size:
4981 2458 80
StyI C'CwwG_G
Cuts at: 513 1065 2349 3114 3589 3816 3976 4284 4357
Size: 552 1284 765 475 227 160 308 73
Cuts at: 4357 4522 4958 513
Size: 165 436 3074
Fragments arranged by size:
3074 1284 765 552 475 436 308 227
165 160 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4182 5546 5563 5716 5901 6346 325
Size: 1255 2602 1364 17 153 185 445 1498
Fragments arranged by size:
2602 1498 1364 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4122 5765 7477
Size: 274 80 33 51 1443 2145 1643 1712
Cuts at: 7477 96
Size: 138
Fragments arranged by size:
2145 1712 1643 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2005 2037 7479 7511 2005
Size: 32 5442 32 2013
Fragments arranged by size:
5442 2013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3842 1316
Size: 2526 4993
XbaI T'CTAG_A
Cuts at: 2545 4855 2545
Size: 2310 5209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 4075 2117
Size: 1958 5561
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7519
XmaI C'CCGG_G
Cuts at: 1870 4844 1870
Size: 2974 4545
XmnI GAAnn'nnTTC
Cuts at: 5648 5648
Size: 7519
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5327 276
Size: 53 83 186 936 3793 2468
Fragments arranged by size:
3793 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI