Restriction map for
pFN21AB4099
(Circular) (Six-base) MAPSORT of: pFN21AB4099.seq Check: 2748 from: 1 to: 6061
pFN21AB4099 6061 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3190 3190
Size: 6061
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 2220 4188 4561 1563
Size: 657 1968 373 3063
Fragments arranged by size:
3063 1968 657 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2121 2664 4124 5508 5776 1632
Size: 421 68 543 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 543 421 268 68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 2101 4790 2101
Size: 2689 3372
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 6029
AlwNI CAG_nnn'CTG
Cuts at: 2855 3804 5376 2855
Size: 949 1572 3540
Fragments arranged by size:
3540 1572 949
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2197 3370 3596 3748 5857 1493
Size: 704 1173 226 152 2109 1697
Fragments arranged by size:
2109 1697 1173 704 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3270 3386 1870
Size: 73 1327 116 4545
Fragments arranged by size:
4545 1327 116 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 3382 4837 618
Size: 325 217 627 633 962 1455 1842
Fragments arranged by size:
1842 1455 962 633 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3380 1391
Size: 1989 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3070 3096 928
Size: 971 1171 26 3893
Fragments arranged by size:
3893 1171 971 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 4122 5278 1790
Size: 2332 1156 2573
Fragments arranged by size:
2573 2332 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2656 2879 3792
Size: 416 53 83 399 24 1239 223 913
Cuts at: 3792 4750 442
Size: 958 1753
Fragments arranged by size:
1753 1239 958 913 416 399 223 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 2196 2594 2899 3013 4721 1239
Size: 844 113 398 305 114 1708 2579
Fragments arranged by size:
2579 1708 844 398 305 114 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2941 4724 882
Size: 617 1442 1783 2219
Fragments arranged by size:
2219 1783 1442 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 2789 2957 6055 2789
Size: 168 3098 2795
Fragments arranged by size:
3098 2795 168
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2116 2146 3011
Size: 30 165 30 678 30 5 30 865
Cuts at: 3011 3041 1178
Size: 30 4198
Fragments arranged by size:
4198 865 678 165 30 30 30 30
30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6061
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2487 4122 4207 5278 2487
Size: 1635 85 1071 3270
Fragments arranged by size:
3270 1635 1071 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
BsmBI CGTCTCn'nnnn_
Cuts at: 3031 3031
Size: 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2487 3380 4122 4207 5278 1391
Size: 399 697 893 742 85 1071 2174
Fragments arranged by size:
2174 1071 893 742 697 399 85
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 3190 3416 844
Size: 1894 452 226 3489
Fragments arranged by size:
3489 1894 452 226
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 4705 4892 1700
Size: 110 200 2695 187 2869
Fragments arranged by size:
2869 2695 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 2561 2699 2787 96
Size: 2465 138 88 3370
Fragments arranged by size:
3370 2465 138 88
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 3099 4121 5133 3099
Size: 1022 1012 4027
Fragments arranged by size:
4027 1022 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2855 794
Size: 2061 4000
BstBI TT'CG_AA
Cuts at: 2468 4865 2468
Size: 2397 3664
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056
Size: 1419 367 385 17 1452 86 12 346
Cuts at: 6056 1972
Size: 1977
Fragments arranged by size:
1977 1452 1419 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2271 2523 513
Size: 552 1206 252 4051
Fragments arranged by size:
4051 1206 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3577 4371 4391 3139
Size: 438 794 20 4809
Fragments arranged by size:
4809 794 438 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 2203 3365 2203
Size: 1162 4899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026
Size: 54 1425 50 275 18 1678 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1678 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2673 3065 3991 1353
Size: 1320 392 926 3423
Fragments arranged by size:
3423 1320 926 392
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2083 2121 2196 2594 2664 2899
Size: 393 421 30 38 75 398 70 235
Cuts at: 2899 3013 4124 4721 5508 5776 1239
Size: 114 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 787 597 421 398 393 268
235 114 75 70 38 30
EcoO109I rG'GnC_Cy
Cuts at: 2207 2207
Size: 6061
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
EcoRV GAT'ATC
Cuts at: 3267 3267
Size: 6061
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2087 2113 2116 2119
Size: 32 135 32 509 6 26 3 3
Cuts at: 2119 2148 3207 3239 3251 3283 4702 4734 4776
Size: 29 1059 32 12 32 1419 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 1419 1059 509 135 42 32 32
32 32 32 32 29 26 12 6
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2963 3345 4019 4378 4537 518
Size: 648 1797 382 674 359 159 2042
Fragments arranged by size:
2042 1797 674 648 382 359 159
MspA1I CmG'CkG
Cuts at: 1606 2572 3014 3167 3477 4154 5302 5547 1606
Size: 966 442 153 310 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 442 310 245 153
NcoI C'CATG_G
Cuts at: 513 1065 2271 2523 513
Size: 552 1206 252 4051
Fragments arranged by size:
4051 1206 552 252
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3212 1108
Size: 2104 3957
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 4130 4162 5643 5675 3011
Size: 32 1087 32 1481 32 3397
Fragments arranged by size:
3397 1481 1087 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2207 2207
Size: 6061
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2572 3167 2572
Size: 595 5466
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2488 3409 4544 5225 5416 826
Size: 219 1443 921 1135 681 191 1471
Fragments arranged by size:
1471 1443 1135 921 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3270 4165 5066 5328 820
Size: 197 926 1327 895 901 262 1553
Fragments arranged by size:
1553 1327 926 901 895 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 2233 2290 3985 5
Size: 47 2181 57 1695 2081
Fragments arranged by size:
2181 2081 1695 57 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2271 2523 3500 513
Size: 552 1206 252 977 3074
Fragments arranged by size:
3074 1206 977 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2915 4088 4105 4258 4443 4888 325
Size: 1255 1335 1173 17 153 185 445 1498
Fragments arranged by size:
1498 1335 1255 1173 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2561 2699 2787
Size: 274 80 33 51 1443 584 138 88
Cuts at: 2787 4307 6019 96
Size: 1520 1712 138
Fragments arranged by size:
1712 1520 1443 584 274 138 138 88
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 3155 2866
Size: 289 5772
XhoI C'TCGA_G
Cuts at: 1943 3270 1943
Size: 1327 4734
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI