Restriction map for
pFN21AB4094
(Circular) (Six-base) MAPSORT of: pFN21AB4094.seq Check: 8313 from: 1 to: 6550
pFN21AB4094 6550 bp
With 182 enzymes: SgfI *
June 13, 2018 20:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3264 3264
Size: 6550
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4360 278
Size: 53 83 186 936 2824 2468
Fragments arranged by size:
2824 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 3893 1937
Size: 370 1586 4594
Fragments arranged by size:
4594 1586 370
Acc65I G'GTAC_C
Cuts at: 2844 3871 2844
Size: 1027 5523
AclI AA'CG_TT
Cuts at: 1563 2175 4677 5050 1563
Size: 612 2502 373 3063
Fragments arranged by size:
3063 2502 612 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2419 3167 3627 4613 5997 6265 1632
Size: 401 386 748 460 986 1384 268 1917
Fragments arranged by size:
1917 1384 986 748 460 401 386 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6353
AflIII A'CryG_T
Cuts at: 1203 2982 5449 6426 1203
Size: 1779 2467 977 1327
Fragments arranged by size:
2467 1779 1327 977
AgeI A'CCGG_T
Cuts at: 5381 5381
Size: 6550
AhdI GACnn_n'nnGTC
Cuts at: 5279 5279
Size: 6550
AleI CACnn'nnGTG
Cuts at: 1166 3308 1166
Size: 2142 4408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6550
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 6518
AlwNI CAG_nnn'CTG
Cuts at: 2521 2806 4293 5865 2521
Size: 285 1487 1572 3206
Fragments arranged by size:
3206 1572 1487 285
ApaI G_GGCC'C
Cuts at: 2419 2583 3467 2419
Size: 164 884 5502
Fragments arranged by size:
5502 884 164
ApaLI G'TGCA_C
Cuts at: 4607 5763 4607
Size: 1156 5394
ApoI r'AATT_y
Cuts at: 1493 3859 4085 4237 6346 1493
Size: 2366 226 152 2109 1697
Fragments arranged by size:
2366 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5104 160
Size: 872 4072 1606
Fragments arranged by size:
4072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2325 3444 3597 3733 3875 1870
Size: 73 237 145 1119 153 136 142 4545
Fragments arranged by size:
4545 1119 237 153 145 142 136 73
AvrII C'CTAG_G
Cuts at: 3349 3349
Size: 6550
BamHI G'GATC_C
Cuts at: 3880 4247 3880
Size: 367 6183
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2465 2844 3206 3533
Size: 325 217 627 513 165 379 362 327
Cuts at: 3533 3628 3871 5326 618
Size: 95 243 1455 1842
Fragments arranged by size:
1842 1455 627 513 379 362 327 325
243 217 165 95
BanII G_rGCy'C
Cuts at: 1391 2187 2419 2459 2583 2892 3467 3495 3869
Size: 796 232 40 124 309 575 28 374
Cuts at: 3869 1391
Size: 4072
Fragments arranged by size:
4072 796 575 374 309 232 124 40
28
BbeI G_GCGC'C
Cuts at: 2304 2304
Size: 6550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2033 3093 928
Size: 971 134 1060 4385
Fragments arranged by size:
4385 1060 971 134
BbvCI CC'TCA_GC
Cuts at: 2390 2601 2941 2390
Size: 211 340 5999
Fragments arranged by size:
5999 340 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 4741 4775 1903
Size: 34 1892 34 878 34 3678
Fragments arranged by size:
3678 1892 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4442 5652 4442
Size: 1210 5340
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2463 2516 2663
Size: 107 122 71 631 605 791 53 147
Cuts at: 2663 3035 3410 5161 136
Size: 372 375 1751 1525
Fragments arranged by size:
1751 1525 791 631 605 375 372 147
122 107 71 53
BglII A'GATC_T
Cuts at: 2872 3096 6545 2872
Size: 224 3449 2877
Fragments arranged by size:
3449 2877 224
BlpI GC'TnA_GC
Cuts at: 2667 3967 2667
Size: 1300 5250
Bme1580I G_kGCm'C
Cuts at: 1790 2419 2583 3209 3467 3536 4611 5767 1790
Size: 629 164 626 258 69 1075 1156 2573
Fragments arranged by size:
2573 1156 1075 629 626 258 164 69
BmgBI CAC'GTC
Cuts at: 1318 2059 1318
Size: 741 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2453 4281 5239
Size: 416 53 83 399 24 1036 1828 958
Cuts at: 5239 442
Size: 1753
Fragments arranged by size:
1828 1753 1036 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2326 2801 2906 3188 3215 5210 1239
Size: 1087 475 105 282 27 1995 2579
Fragments arranged by size:
2579 1995 1087 475 282 105 27
Bpu10I CC'TnA_GC
Cuts at: 2390 2601 2941 3624 2390
Size: 211 340 683 5316
Fragments arranged by size:
5316 683 340 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3812 4675 5540 5838 3812
Size: 863 865 298 4524
Fragments arranged by size:
4524 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2371 2531 5213 882
Size: 617 872 160 2682 2219
Fragments arranged by size:
2682 2219 872 617 160
BsaAI yAC'GTr
Cuts at: 493 1204 3319 4341 493
Size: 711 2115 1022 2702
Fragments arranged by size:
2702 2115 1022 711
BsaBI GATnn'nnATC
Cuts at: 6544 6544
Size: 6550
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 4357 4739 5374
Size: 53 83 186 936 768 2056 382 635
Cuts at: 5374 275
Size: 1451
Fragments arranged by size:
2056 1451 936 768 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2483 4983 5381 5655 5802 6213 2483
Size: 2500 398 274 147 411 2820
Fragments arranged by size:
2820 2500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 1178
Size: 30 165 30 1311 30 4984
Fragments arranged by size:
4984 1311 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2894 3107 3194 3305 3437 3503 1186
Size: 922 786 213 87 111 132 66 4233
Fragments arranged by size:
4233 922 786 213 132 111 87 66
BseYI C'CCAG_C
Cuts at: 2083 2125 2198 3324 5753 6249 2083
Size: 42 73 1126 2429 496 2384
Fragments arranged by size:
2429 2384 1126 496 73 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 3299 3362 3567 3794 1163
Size: 573 1014 549 63 205 227 3919
Fragments arranged by size:
3919 1014 573 549 227 205 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3188 3414 4000 4761 5789 1540
Size: 275 186 1187 226 586 761 1028 2301
Fragments arranged by size:
2301 1187 1028 761 586 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2187 3672 4611 4696 5767 2112
Size: 75 1485 939 85 1071 2895
Fragments arranged by size:
2895 1485 1071 939 85 75
BsmI GAATG_Cn'
Cuts at: 4061 4154 4061
Size: 93 6457
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2187 2419 2459 2583 2892 3209
Size: 399 322 75 232 40 124 309 317
Cuts at: 3209 3467 3495 3536 3672 3869 4611 4696 5767
Size: 258 28 41 136 197 742 85 1071
Cuts at: 5767 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 322 317 309 258
232 197 136 124 85 75 41 40
28
BspEI T'CCGG_A
Cuts at: 6213 6213
Size: 6550
BspHI T'CATG_A
Cuts at: 4439 6169 4439
Size: 1730 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2772 3150 3264 3905 844
Size: 1928 378 114 641 3489
Fragments arranged by size:
3489 1928 641 378 114
BsrBI CCG'CTC
Cuts at: 1394 1823 2397 2671 3184 4437 1394
Size: 429 574 274 513 1253 3507
Fragments arranged by size:
3507 1253 574 513 429 274
BsrDI GCAATG_nn'
Cuts at: 65 1248 2934 3022 5045 5219 65
Size: 1183 1686 88 2023 174 1396
Fragments arranged by size:
2023 1686 1396 1183 174 88
BsrFI r'CCGG_y
Cuts at: 1700 1810 2657 2901 3453 3536 5194 5381 1700
Size: 110 847 244 552 83 1658 187 2869
Fragments arranged by size:
2869 1658 847 552 244 187 110 83
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6550
BssSI C'ACGA_G
Cuts at: 4610 5622 4610
Size: 1012 5538
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2806 3382 794
Size: 2012 576 3962
Fragments arranged by size:
3962 2012 576
BstBI TT'CG_AA
Cuts at: 5354 5354
Size: 6550
BstEII G'GTnAC_C
Cuts at: 1184 2605 1184
Size: 1421 5129
BstXI CCAn_nnnn'nTGG
Cuts at: 3166 3166
Size: 6550
BstYI r'GATC_y
Cuts at: 1972 2292 2872 3096 3880 4247 4632 4649 6101
Size: 320 580 224 784 367 385 17 1452
Cuts at: 6101 6187 6199 6545 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 784 580 385 367 346 320
224 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2857 2857
Size: 6550
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 2850 3428 3467 513
Size: 552 939 237 609 578 39 3596
Fragments arranged by size:
3596 939 609 578 552 237 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2245 487
Size: 683 56 1019 4792
Fragments arranged by size:
4792 1019 683 56
BtsI GCAGTG_nn'
Cuts at: 3555 4066 4860 4880 3555
Size: 511 794 20 5225
Fragments arranged by size:
5225 794 511 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3319 3354 529
Size: 35 2755 35 3725
Fragments arranged by size:
3725 2755 35 35
DraI TTT'AAA
Cuts at: 3854 3854
Size: 6550
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5557 809
Size: 507 4241 1802
Fragments arranged by size:
4241 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 2899 2903
Size: 54 1425 50 275 18 414 655 4
Cuts at: 2903 3051 3185 3411 3538 3717 3997 4886 6515
Size: 148 134 226 127 179 280 889 1629
Cuts at: 6515 8
Size: 43
Fragments arranged by size:
1629 1425 889 655 414 280 275 226
179 148 134 127 54 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 3185 3411 3997 1537
Size: 275 1373 226 586 4090
Fragments arranged by size:
4090 1373 586 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 2438 4480 1353
Size: 1085 2042 3423
Fragments arranged by size:
3423 2042 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3059 3214 5137 5511 5657 387
Size: 1064 138 1470 155 1923 374 146 1280
Fragments arranged by size:
1923 1470 1280 1064 374 155 146 138
EcoICRI GAG'CTC
Cuts at: 2185 2185
Size: 6550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2326 2419 2801 2906 3167 3188
Size: 393 401 293 93 382 105 261 21
Cuts at: 3188 3215 3627 4613 5210 5997 6265 1239
Size: 27 412 986 597 787 268 1524
Fragments arranged by size:
1524 986 787 597 412 401 393 382
293 268 261 105 93 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2328 2552 3146 2328
Size: 224 594 5732
Fragments arranged by size:
5732 594 224
EcoO109I rG'GnC_Cy
Cuts at: 2508 2518 3235 3463 3526 3557 3602 2508
Size: 10 717 228 63 31 45 5456
Fragments arranged by size:
5456 717 228 63 45 31 10
EcoRI G'AATT_C
Cuts at: 3859 4237 3859
Size: 378 6172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 6550
FseI GG_CCGG'CC
Cuts at: 2905 2905
Size: 6550
FspI TGC'GCA
Cuts at: 3386 5056 3386
Size: 1670 4880
FspAI rTGC'GCAy
Cuts at: 3386 3386
Size: 6550
HaeII r_GCGC'y
Cuts at: 1678 2304 3741 5697 1678
Size: 626 1437 1956 2531
Fragments arranged by size:
2531 1956 1437 626
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2289 2321 2941 2973 5191
Size: 32 135 32 717 32 620 32 2218
Cuts at: 5191 5223 5265 5297 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2218 717 620 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2155 2308 3894 4140 669
Size: 988 281 217 153 1586 246 3079
Fragments arranged by size:
3079 1586 988 281 246 217 153
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6550
HpaI GTT'AAC
Cuts at: 4140 4140
Size: 6550
KasI G'GCGC_C
Cuts at: 2300 2300
Size: 6550
KpnI G_GTAC'C
Cuts at: 2848 3875 2848
Size: 1027 5523
MfeI C'AATT_G
Cuts at: 4149 4149
Size: 6550
MluI A'CGCG_T
Cuts at: 6426 6426
Size: 6550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2432 5664 5848 1305
Size: 1127 3232 184 2007
Fragments arranged by size:
3232 2007 1127 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3719 10
Size: 54 1425 343 1887 2841
Fragments arranged by size:
2841 1887 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3308 4508 4867 5026 518
Size: 648 2142 1200 359 159 2042
Fragments arranged by size:
2142 2042 1200 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2600 2794 3966 4643 5791 6036 1606
Size: 435 559 194 1172 677 1148 245 2120
Fragments arranged by size:
2120 1172 1148 677 559 435 245 194
NaeI GCC'GGC
Cuts at: 2903 3455 2903
Size: 552 5998
NarI GG'CG_CC
Cuts at: 2301 2301
Size: 6550
NcoI C'CATG_G
Cuts at: 513 1065 2004 2850 513
Size: 552 939 846 4213
Fragments arranged by size:
4213 939 846 552
NdeI CA'TA_TG
Cuts at: 387 2378 387
Size: 1991 4559
NgoMIV G'CCGG_C
Cuts at: 2901 3453 2901
Size: 552 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6550
NotI GC'GGCC_GC
Cuts at: 3997 3997
Size: 6550
NspI r_CATG'y
Cuts at: 1132 1229 2704 2986 3908 5453 1132
Size: 97 1475 282 922 1545 2229
Fragments arranged by size:
2229 1545 1475 922 282 97
PasI CC'CwG_GG
Cuts at: 1348 2521 1348
Size: 1173 5377
PciI A'CATG_T
Cuts at: 2982 5449 2982
Size: 2467 4083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 1108
Size: 1413 5137
PfoI T'CCnGG_A
Cuts at: 2529 3063 3231 2529
Size: 534 168 5848
Fragments arranged by size:
5848 534 168
PmeI GTTT'AAAC
Cuts at: 3854 3854
Size: 6550
PmlI CAC'GTG
Cuts at: 3319 3319
Size: 6550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 3205 3237 4619 4651 6132 6164 2714
Size: 32 459 32 1382 32 1481 32 3100
Fragments arranged by size:
3100 1481 1382 459 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3235 3557 3235
Size: 322 6228
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6550
PsiI TTA'TAA
Cuts at: 4120 4120
Size: 6550
PspOMI G'GGCC_C
Cuts at: 2415 2579 3463 2415
Size: 164 884 5502
Fragments arranged by size:
5502 884 164
PspXI vC'TCGA_Gb
Cuts at: 3733 3733
Size: 6550
PstI C_TGCA'G
Cuts at: 830 2793 2799 3036 3902 830
Size: 1963 6 237 866 3478
Fragments arranged by size:
3478 1963 866 237 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6550
PvuII CAG'CTG
Cuts at: 2600 2794 2600
Size: 194 6356
SacI G_AGCT'C
Cuts at: 2187 2187
Size: 6550
SalI G'TCGA_C
Cuts at: 1936 2306 3892 1936
Size: 370 1586 4594
Fragments arranged by size:
4594 1586 370
SbfI CC_TGCA'GG
Cuts at: 3036 3902 3036
Size: 866 5684
ScaI AGT'ACT
Cuts at: 4798 6510 4798
Size: 1712 4838
SexAI A'CCwGG_T
Cuts at: 2103 2103
Size: 6550
SfcI C'TryA_G
Cuts at: 826 1045 2789 2795 3032 3898 5033 5714 5905
Size: 219 1744 6 237 866 1135 681 191
Cuts at: 5905 826
Size: 1471
Fragments arranged by size:
1744 1471 1135 866 681 237 219 191
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2516 3410 2516
Size: 894 5656
SfoI GGC'GCC
Cuts at: 2302 2302
Size: 6550
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6550
SmaI CCC'GGG
Cuts at: 1872 3446 3877 1872
Size: 1574 431 4545
Fragments arranged by size:
4545 1574 431
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2325 3733 3827 4654 5555 5817
Size: 197 926 382 1408 94 827 901 262
Cuts at: 5817 820
Size: 1553
Fragments arranged by size:
1553 1408 926 901 827 382 262 197
94
SnaBI TAC'GTA
Cuts at: 493 4341 493
Size: 3848 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6550
SphI G_CATG'C
Cuts at: 1132 3908 1132
Size: 2776 3774
SspI AAT'ATT
Cuts at: 5 52 4474 5
Size: 47 4422 2081
Fragments arranged by size:
4422 2081 47
StuI AGG'CCT
Cuts at: 1517 3038 3846 1517
Size: 1521 808 4221
Fragments arranged by size:
4221 1521 808
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2850 3217 3349 3989 513
Size: 552 939 114 732 367 132 640 3074
Fragments arranged by size:
3074 939 732 640 552 367 132 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3224 4577 4594 4747 4932 5377 325
Size: 1255 1644 1353 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1353 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4796 6508 96
Size: 274 80 33 51 1443 2819 1712 138
Fragments arranged by size:
2819 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 2411 2443 3227 3259 6510 6542 2221
Size: 32 158 32 784 32 3251 32 2229
Fragments arranged by size:
3251 2229 784 158 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6550
XbaI T'CTAG_A
Cuts at: 2875 3886 2875
Size: 1011 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2056 2350 2056
Size: 294 6256
XhoI C'TCGA_G
Cuts at: 1943 2325 3733 1943
Size: 382 1408 4760
Fragments arranged by size:
4760 1408 382
XmaI C'CCGG_G
Cuts at: 1870 3444 3875 1870
Size: 1574 431 4545
Fragments arranged by size:
4545 1574 431
XmnI GAAnn'nnTTC
Cuts at: 4679 4679
Size: 6550
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4358 276
Size: 53 83 186 936 2824 2468
Fragments arranged by size:
2824 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I
DraIII EcoRV NruI NsiI PacI PsrI RsrII SacII
SanDI SapI SrfI SwaI