Restriction map for
pFN21AB4092
(Circular) (Six-base) MAPSORT of: pFN21AB4092.seq Check: 3369 from: 1 to: 5950
pFN21AB4092 5950 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2117 2117
Size: 5950
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 4013 5397 5665 1632
Size: 989 1392 1384 268 1917
Fragments arranged by size:
1917 1392 1384 989 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 2244 4781 2244
Size: 2537 3413
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 2802 3693 5265 2802
Size: 891 1572 3487
Fragments arranged by size:
3487 1572 891
ApaI G_GGCC'C
Cuts at: 2018 2631 2018
Size: 613 5337
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3088 3259 3485 3637 5746 1493
Size: 1595 171 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 171 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2642 3275 1870
Size: 73 699 633 4545
Fragments arranged by size:
4545 699 633 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 3271 4726 618
Size: 325 217 627 918 566 1455 1842
Fragments arranged by size:
1842 1455 918 627 566 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2631 3269 1391
Size: 627 613 638 4072
Fragments arranged by size:
4072 638 627 613
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4979
BbvCI CC'TCA_GC
Cuts at: 2795 2795
Size: 5950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2538 2631 4011 5167 1790
Size: 228 520 93 1380 1156 2573
Fragments arranged by size:
2573 1380 1156 520 228 93
BmgBI CAC'GTC
Cuts at: 1318 2083 1318
Size: 765 5185
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3200 4610 1239
Size: 1961 1410 2579
Fragments arranged by size:
2579 1961 1410
Bpu10I CC'TnA_GC
Cuts at: 2795 2795
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2784 4613 882
Size: 617 1285 1829 2219
Fragments arranged by size:
2219 1829 1285 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2244 2810 4383 4781 5055 5202 5613 2244
Size: 566 1573 398 274 147 411 2581
Fragments arranged by size:
2581 1573 566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2792 2822 3173 3203 1178
Size: 30 165 30 1389 30 351 30 3925
Fragments arranged by size:
3925 1389 351 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2750 2925 1186
Size: 1564 175 4211
Fragments arranged by size:
4211 1564 175
BseYI C'CCAG_C
Cuts at: 2011 5153 5649 2011
Size: 3142 496 2312
Fragments arranged by size:
3142 2312 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2105 2495 2636 2873 1163
Size: 573 353 16 390 141 237 4240
Fragments arranged by size:
4240 573 390 353 237 141 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2907 3400 4161 5189 1540
Size: 275 186 906 493 761 1028 2301
Fragments arranged by size:
2301 1028 906 761 493 275 186
BsiHKAI G_wGCw'C
Cuts at: 2861 4011 4096 5167 2861
Size: 1150 85 1071 3644
Fragments arranged by size:
3644 1150 1071 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2538 2631 2861 3269 4011 4096
Size: 399 228 520 93 230 408 742 85
Cuts at: 4096 5167 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 520 408 399 230 228
93 85
BspEI T'CCGG_A
Cuts at: 2810 5613 2810
Size: 2803 3147
BspHI T'CATG_A
Cuts at: 2113 3016 3839 5569 2113
Size: 903 823 1730 2494
Fragments arranged by size:
2494 1730 903 823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 3305 844
Size: 1273 1188 3489
Fragments arranged by size:
3489 1273 1188
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2429 3115 4445 4619 65
Size: 1183 1181 686 1330 174 1396
Fragments arranged by size:
1396 1330 1183 1181 686 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2244 2637 3212 4594 4781 1700
Size: 110 434 393 575 1382 187 2869
Fragments arranged by size:
2869 1382 575 434 393 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 2590 3280 3647 4032 4049 5501 5587 5599
Size: 618 690 367 385 17 1452 86 12
Cuts at: 5599 5945 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 690 618 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2019 2019
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2050 513
Size: 552 985 4413
Fragments arranged by size:
4413 985 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 4260 4280 3466
Size: 794 20 5136
Fragments arranged by size:
5136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2032 2067 529
Size: 35 1468 35 4412
Fragments arranged by size:
4412 1468 35 35
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2621 3397 4286
Size: 54 1425 50 275 18 791 776 889
Cuts at: 4286 5915 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 791 776 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2974 3086 3880 1353
Size: 1621 112 794 3423
Fragments arranged by size:
3423 1621 794 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2621 3200 4013 4610 5397 5665 1239
Size: 393 989 579 813 597 787 268 1524
Fragments arranged by size:
1524 989 813 787 597 579 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2022 2022
Size: 5950
EcoO109I rG'GnC_Cy
Cuts at: 2015 2627 2628 2887 2015
Size: 612 1 259 5078
Fragments arranged by size:
5078 612 259 1
EcoRI G'AATT_C
Cuts at: 3088 3259 3637 3088
Size: 171 378 5401
Fragments arranged by size:
5401 378 171
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2328 5097 1678
Size: 650 2769 2531
Fragments arranged by size:
2769 2531 650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3173 3205 4591 4623 4665
Size: 32 135 32 1601 32 1386 32 42
Cuts at: 4665 4697 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 1386 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 5064 5248 1305
Size: 1080 2679 184 2007
Fragments arranged by size:
2679 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4128
Fragments arranged by size:
4128 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2365 2549 2612 3146 3908 4267
Size: 648 1089 110 184 63 534 762 359
Cuts at: 4267 4426 518
Size: 159 2042
Fragments arranged by size:
2042 1089 762 648 534 359 184 159
110 63
MspA1I CmG'CkG
Cuts at: 1606 2620 3366 4043 5191 5436 1606
Size: 1014 746 677 1148 245 2120
Fragments arranged by size:
2120 1148 1014 746 677 245
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5398
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 3308 4853 1132
Size: 97 2079 1545 2229
Fragments arranged by size:
2229 2079 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2113 4019 4051 5532 5564 2081
Size: 32 1906 32 1481 32 2467
Fragments arranged by size:
2467 1906 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2014 2627 2014
Size: 613 5337
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 5918
PstI C_TGCA'G
Cuts at: 830 3302 830
Size: 2472 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2710 3217 3298 4433 5114 5305 826
Size: 219 1665 507 81 1135 681 191 1471
Fragments arranged by size:
1665 1471 1135 681 507 219 191 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 2644 3277 1872
Size: 772 633 4545
Fragments arranged by size:
4545 772 633
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 2685 2701 1517
Size: 1168 16 4766
Fragments arranged by size:
4766 1168 16
StyI C'CwwG_G
Cuts at: 513 1065 3389 513
Size: 552 2324 3074
Fragments arranged by size:
3074 2324 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 2921 3227 3977 3994 4147 4332
Size: 1255 698 643 306 750 17 153 185
Cuts at: 4332 4777 325
Size: 445 1498
Fragments arranged by size:
1498 1255 750 698 643 445 306 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 4196 5908
Size: 274 80 33 51 1443 762 1457 1712
Cuts at: 5908 96
Size: 138
Fragments arranged by size:
1712 1457 1443 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2963 3194 2963
Size: 231 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 2642 3275 1870
Size: 772 633 4545
Fragments arranged by size:
4545 772 633
XmnI GAAnn'nnTTC
Cuts at: 2385 4079 2385
Size: 1694 4256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI