Restriction map for
pFN21AB4089
(Circular) (Six-base) MAPSORT of: pFN21AB4089.seq Check: 6590 from: 1 to: 9373
pFN21AB4089 9373 bp
With 182 enzymes: SgfI *
September 19, 2018 16:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7183 278
Size: 53 83 186 936 5647 2468
Fragments arranged by size:
5647 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2179 5590 6716 1937
Size: 242 3411 1126 4594
Fragments arranged by size:
4594 3411 1126 242
Acc65I G'GTAC_C
Cuts at: 6694 6694
Size: 9373
AclI AA'CG_TT
Cuts at: 1563 5602 7500 7873 1563
Size: 4039 1898 373 3063
Fragments arranged by size:
4039 3063 1898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2708 2794 3483 3666 4218 4524 4836
Size: 590 486 86 689 183 552 306 312
Cuts at: 4836 5583 6118 7436 8820 9088 1632
Size: 747 535 1318 1384 268 1917
Fragments arranged by size:
1917 1384 1318 747 689 590 552 535
486 312 306 268 183 86
AflII C'TTAA_G
Cuts at: 820 1017 3224 4028 820
Size: 197 2207 804 6165
Fragments arranged by size:
6165 2207 804 197
AflIII A'CryG_T
Cuts at: 1203 2458 8272 9249 1203
Size: 1255 5814 977 1327
Fragments arranged by size:
5814 1327 1255 977
AgeI A'CCGG_T
Cuts at: 8204 8204
Size: 9373
AhdI GACnn_n'nnGTC
Cuts at: 6267 6611 8102 6267
Size: 344 1491 7538
Fragments arranged by size:
7538 1491 344
AleI CACnn'nnGTG
Cuts at: 1166 3815 1166
Size: 2649 6724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9373
AlwNI CAG_nnn'CTG
Cuts at: 3689 7116 8688 3689
Size: 3427 1572 4374
Fragments arranged by size:
4374 3427 1572
ApaLI G'TGCA_C
Cuts at: 4794 7430 8586 4794
Size: 2636 1156 5581
Fragments arranged by size:
5581 2636 1156
ApoI r'AATT_y
Cuts at: 1493 2313 4002 4090 4268 4823 6682 6908 7060
Size: 820 1689 88 178 555 1859 226 152
Cuts at: 7060 9169 1493
Size: 2109 1697
Fragments arranged by size:
2109 1859 1697 1689 820 555 226 178
152 88
AseI AT'TA_AT
Cuts at: 160 1032 7927 160
Size: 872 6895 1606
Fragments arranged by size:
6895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9373
AvaI C'yCGr_G
Cuts at: 1870 1943 6698 1870
Size: 73 4755 4545
Fragments arranged by size:
4755 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2177 2210 2177
Size: 33 9340
BamHI G'GATC_C
Cuts at: 6703 7070 6703
Size: 367 9006
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6694 8149 618
Size: 325 217 627 4907 1455 1842
Fragments arranged by size:
4907 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4371 6692 1391
Size: 2980 2321 4072
Fragments arranged by size:
4072 2980 2321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4512 5109 5617 5667 5803 928
Size: 971 2613 597 508 50 136 4498
Fragments arranged by size:
4498 2613 971 597 508 136 50
BbvCI CC'TCA_GC
Cuts at: 4845 4845
Size: 9373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7564 7598 1903
Size: 34 50 34 5543 34 3678
Fragments arranged by size:
5543 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4579 7265 8475 4579
Size: 2686 1210 5477
Fragments arranged by size:
5477 2686 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9373
BfrBI ATG'CAT
Cuts at: 3314 4892 3314
Size: 1578 7795
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7984 136
Size: 107 122 71 631 605 6312 1525
Fragments arranged by size:
6312 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2100 4880 6170 9368 2100
Size: 2780 1290 3198 2105
Fragments arranged by size:
3198 2780 2105 1290
BlpI GC'TnA_GC
Cuts at: 6790 6790
Size: 9373
Bme1580I G_kGCm'C
Cuts at: 1790 4798 7434 8590 1790
Size: 3008 2636 1156 2573
Fragments arranged by size:
3008 2636 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 6613 1318
Size: 5295 4078
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3780 6275 6615
Size: 416 53 83 399 24 2363 2495 340
Cuts at: 6615 7104 8062 442
Size: 489 958 1753
Fragments arranged by size:
2495 2363 1753 958 489 416 399 340
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4805 1056
Size: 3749 5624
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5376 5378 5376
Size: 2 9371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4602 5041 6228 8033 1239
Size: 3363 439 1187 1805 2579
Fragments arranged by size:
3363 2579 1805 1187 439
Bpu10I CC'TnA_GC
Cuts at: 4845 4845
Size: 9373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 4539 5534 5745 5836 7498 8363 8661 2228
Size: 2311 995 211 91 1662 865 298 2940
Fragments arranged by size:
2940 2311 1662 995 865 298 211 91
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2830 5623 8036 882
Size: 617 1331 2793 2413 2219
Fragments arranged by size:
2793 2413 2219 1331 617
BsaAI yAC'GTr
Cuts at: 493 1204 3149 4631 7164 493
Size: 711 1945 1482 2533 2702
Fragments arranged by size:
2702 2533 1945 1482 711
BsaBI GATnn'nnATC
Cuts at: 9367 9367
Size: 9373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7180 7562 8197 275
Size: 53 83 186 936 5647 382 635 1451
Fragments arranged by size:
5647 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7806 8204 8478 8625 9036 7806
Size: 398 274 147 411 8143
Fragments arranged by size:
8143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5164 5194 5714 5744 6183
Size: 30 165 30 3761 30 520 30 439
Cuts at: 6183 6213 1178
Size: 30 4338
Fragments arranged by size:
4338 3761 520 439 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3747 5608 5735 5958 6309 1186
Size: 2561 1861 127 223 351 4250
Fragments arranged by size:
4250 2561 1861 351 223 127
BseYI C'CCAG_C
Cuts at: 8576 9072 8576
Size: 496 8877
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3543 3628 3653 4664 4935 1163
Size: 573 1807 85 25 1011 271 5601
Fragments arranged by size:
5601 1807 1011 573 271 85 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4429 6823 7584 8612 1540
Size: 275 186 2428 2394 761 1028 2301
Fragments arranged by size:
2428 2394 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4371 4798 5909 7434 7519 8590 4371
Size: 427 1111 1525 85 1071 5154
Fragments arranged by size:
5154 1525 1111 1071 427 85
BsmI GAATG_Cn'
Cuts at: 5452 6884 6977 5452
Size: 1432 93 7848
Fragments arranged by size:
7848 1432 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4371 4798 5909 6692 7434 7519 8590
Size: 399 2581 427 1111 783 742 85 1071
Cuts at: 8590 1391
Size: 2174
Fragments arranged by size:
2581 2174 1111 1071 783 742 427 399
85
BspEI T'CCGG_A
Cuts at: 9036 9036
Size: 9373
BspHI T'CATG_A
Cuts at: 6301 7262 8992 6301
Size: 961 1730 6682
Fragments arranged by size:
6682 1730 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 6728 844
Size: 1220 4664 3489
Fragments arranged by size:
4664 3489 1220
BsrBI CCG'CTC
Cuts at: 1394 1823 5281 7260 1394
Size: 429 3458 1979 3507
Fragments arranged by size:
3507 3458 1979 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5253 6141 6423 7868 8042 65
Size: 1183 4005 888 282 1445 174 1396
Fragments arranged by size:
4005 1445 1396 1183 888 282 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6649 8017 8204 1700
Size: 110 4839 1368 187 2869
Fragments arranged by size:
4839 2869 1368 187 110
BsrGI T'GTAC_A
Cuts at: 96 4546 96
Size: 4450 4923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9373
BssSI C'ACGA_G
Cuts at: 2664 7433 8445 2664
Size: 4769 1012 3592
Fragments arranged by size:
4769 3592 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3566 794
Size: 2772 6601
BstBI TT'CG_AA
Cuts at: 6512 8177 6512
Size: 1665 7708
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9373
BstXI CCAn_nnnn'nTGG
Cuts at: 4677 6023 6628 4677
Size: 1346 605 7422
Fragments arranged by size:
7422 1346 605
BstYI r'GATC_y
Cuts at: 1972 2100 3631 4558 4880 6170 6703 7070 7455
Size: 128 1531 927 322 1290 533 367 385
Cuts at: 7455 7472 8924 9010 9022 9368 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1531 1452 1290 927 533 385 367
346 322 128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4563 4563
Size: 9373
BtgI C'CryG_G
Cuts at: 513 1065 2049 513
Size: 552 984 7837
Fragments arranged by size:
7837 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5493 5712 6237 487
Size: 683 56 4267 219 525 3623
Fragments arranged by size:
4267 3623 683 525 219 56
BtsI GCAGTG_nn'
Cuts at: 2408 5233 6029 6889 7683 7703 2408
Size: 2825 796 860 794 20 4078
Fragments arranged by size:
4078 2825 860 796 794 20
ClaI AT'CG_AT
Cuts at: 1554 2347 1554
Size: 793 8580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9338
DraI TTT'AAA
Cuts at: 4264 6677 4264
Size: 2413 6960
DraIII CAC_nnn'GTG
Cuts at: 3416 3416
Size: 9373
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8380 809
Size: 507 7064 1802
Fragments arranged by size:
7064 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3593 5860 6283
Size: 54 1425 50 275 18 1763 2267 423
Cuts at: 6283 6820 7709 9338 8
Size: 537 889 1629 43
Fragments arranged by size:
2267 1763 1629 1425 889 537 423 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6820 1537
Size: 275 5008 4090
Fragments arranged by size:
5008 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2824 3072 3465 4476 4812 5106 5721 5760
Size: 1471 248 393 1011 336 294 615 39
Cuts at: 5760 5848 7303 1353
Size: 88 1455 3423
Fragments arranged by size:
3423 1471 1455 1011 615 393 336 294
248 88 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 7960 8334 8480 387
Size: 1064 138 507 5864 374 146 1280
Fragments arranged by size:
5864 1280 1064 507 374 146 138
EcoICRI GAG'CTC
Cuts at: 4369 4369
Size: 9373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2708 2794 3483 3666 4218 4524
Size: 393 590 486 86 689 183 552 306
Cuts at: 4524 4602 4836 5041 5583 6118 6228 7436 8033
Size: 78 234 205 542 535 110 1208 597
Cuts at: 8033 8820 9088 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1208 787 689 597 590 552 542
535 486 393 306 268 234 205 183
110 86 78
EcoO109I rG'GnC_Cy
Cuts at: 6391 6391
Size: 9373
EcoRI G'AATT_C
Cuts at: 4002 6682 7060 4002
Size: 2680 378 6315
Fragments arranged by size:
6315 2680 378
EcoRV GAT'ATC
Cuts at: 2326 3722 2326
Size: 1396 7977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2544 3128 4043 5174 5318 5411 5904 2544
Size: 584 915 1131 144 93 493 6013
Fragments arranged by size:
6013 1131 915 584 493 144 93
FspI TGC'GCA
Cuts at: 7879 7879
Size: 9373
HaeII r_GCGC'y
Cuts at: 1678 8520 1678
Size: 6842 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2340 2372 3657 3683 3689
Size: 32 135 32 768 32 1285 26 6
Cuts at: 3689 3715 4202 4234 5532 5564 6197 6229 6253
Size: 26 487 32 1298 32 633 32 24
Cuts at: 6253 6285 6597 6629 8014 8046 8088 8120 1373
Size: 32 312 32 1385 32 42 32 2626
Fragments arranged by size:
2626 1385 1298 1285 768 633 487 312
135 42 32 32 32 32 32 32
32 32 32 32 26 26 24 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2464 5591 6717 6963 669
Size: 988 281 526 3127 1126 246 3079
Fragments arranged by size:
3127 3079 1126 988 526 281 246
HindIII A'AGCT_T
Cuts at: 748 2678 6599 748
Size: 1930 3921 3522
Fragments arranged by size:
3921 3522 1930
HpaI GTT'AAC
Cuts at: 2464 6963 2464
Size: 4499 4874
KpnI G_GTAC'C
Cuts at: 6698 6698
Size: 9373
MfeI C'AATT_G
Cuts at: 2556 6972 2556
Size: 4416 4957
MluI A'CGCG_T
Cuts at: 9249 9249
Size: 9373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 5330 8487 8671 1305
Size: 3330 695 3157 184 2007
Fragments arranged by size:
3330 3157 2007 695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3595 5862 10
Size: 54 1425 343 1763 2267 3521
Fragments arranged by size:
3521 2267 1763 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2439 3815 6021 7331 7690 7849
Size: 648 949 324 1376 2206 1310 359 159
Cuts at: 7849 518
Size: 2042
Fragments arranged by size:
2206 2042 1376 1310 949 648 359 324
159
MspA1I CmG'CkG
Cuts at: 1606 2011 2051 3587 3995 4648 6357 6789 7466
Size: 405 40 1536 408 653 1709 432 677
Cuts at: 7466 8614 8859 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1709 1536 1148 677 653 432 408
405 245 40
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8821
NdeI CA'TA_TG
Cuts at: 387 2335 4894 387
Size: 1948 2559 4866
Fragments arranged by size:
4866 2559 1948
NheI G'CTAG_C
Cuts at: 1052 4801 1052
Size: 3749 5624
NotI GC'GGCC_GC
Cuts at: 6820 6820
Size: 9373
NruI TCG'CGA
Cuts at: 4425 4425
Size: 9373
NsiI A_TGCA'T
Cuts at: 3316 4894 3316
Size: 1578 7795
NspI r_CATG'y
Cuts at: 1132 1229 2462 4435 4513 6731 8276 1132
Size: 97 1233 1973 78 2218 1545 2229
Fragments arranged by size:
2229 2218 1973 1545 1233 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9373
PciI A'CATG_T
Cuts at: 2458 8272 2458
Size: 5814 3559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2711 1108
Size: 1603 7770
PfoI T'CCnGG_A
Cuts at: 3948 4465 3948
Size: 517 8856
PmeI GTTT'AAAC
Cuts at: 6677 6677
Size: 9373
PmlI CAC'GTG
Cuts at: 4631 4631
Size: 9373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7442 7474 8955 8987 7442
Size: 32 1481 32 7828
Fragments arranged by size:
7828 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6391 6391
Size: 9373
PshAI GACnn'nnGTC
Cuts at: 1613 4623 1613
Size: 3010 6363
PsiI TTA'TAA
Cuts at: 5331 6943 5331
Size: 1612 7761
PstI C_TGCA'G
Cuts at: 830 3571 3617 6725 830
Size: 2741 46 3108 3478
Fragments arranged by size:
3478 3108 2741 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9373
PvuII CAG'CTG
Cuts at: 3587 3995 4648 6357 3587
Size: 408 653 1709 6603
Fragments arranged by size:
6603 1709 653 408
SacI G_AGCT'C
Cuts at: 4371 4371
Size: 9373
SacII CC_GC'GG
Cuts at: 2052 2052
Size: 9373
SalI G'TCGA_C
Cuts at: 1936 5589 6715 1936
Size: 3653 1126 4594
Fragments arranged by size:
4594 3653 1126
SapI GCTCTTCn'nnn_
Cuts at: 5848 5848
Size: 9373
SbfI CC_TGCA'GG
Cuts at: 6725 6725
Size: 9373
ScaI AGT'ACT
Cuts at: 5188 7621 9333 5188
Size: 2433 1712 5228
Fragments arranged by size:
5228 2433 1712
SfcI C'TryA_G
Cuts at: 826 1045 3567 3613 3747 5973 6721 7856 8537
Size: 219 2522 46 134 2226 748 1135 681
Cuts at: 8537 8728 826
Size: 191 1471
Fragments arranged by size:
2522 2226 1471 1135 748 681 219 191
134 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9373
SmaI CCC'GGG
Cuts at: 1872 6700 1872
Size: 4828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 3224 4028 4554 5549 5760
Size: 197 926 264 1017 804 526 995 211
Cuts at: 5760 5851 7477 8378 8640 820
Size: 91 1626 901 262 1553
Fragments arranged by size:
1626 1553 1017 995 926 901 804 526
264 262 211 197 91
SnaBI TAC'GTA
Cuts at: 493 7164 493
Size: 6671 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9373
SphI G_CATG'C
Cuts at: 1132 6731 1132
Size: 5599 3774
SspI AAT'ATT
Cuts at: 5 52 6181 7297 5
Size: 47 6129 1116 2081
Fragments arranged by size:
6129 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9373
StyI C'CwwG_G
Cuts at: 513 1065 4416 4621 4978 5409 5637 6812 513
Size: 552 3351 205 357 431 228 1175 3074
Fragments arranged by size:
3351 3074 1175 552 431 357 228 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2812 5926 6634 7400 7417 7570 7755
Size: 1255 1232 3114 708 766 17 153 185
Cuts at: 7755 8200 325
Size: 445 1498
Fragments arranged by size:
3114 1498 1255 1232 766 708 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4546 5186 5207
Size: 274 80 33 51 1443 2569 640 21
Cuts at: 5207 7619 9331 96
Size: 2412 1712 138
Fragments arranged by size:
2569 2412 1712 1443 640 274 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2374 2406 9333 9365 2374
Size: 32 6927 32 2382
Fragments arranged by size:
6927 2382 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9373
XbaI T'CTAG_A
Cuts at: 4733 5606 5621 6709 4733
Size: 873 15 1088 7397
Fragments arranged by size:
7397 1088 873 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3863 3863
Size: 9373
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9373
XmaI C'CCGG_G
Cuts at: 1870 6698 1870
Size: 4828 4545
XmnI GAAnn'nnTTC
Cuts at: 4882 6277 7502 4882
Size: 1395 1225 6753
Fragments arranged by size:
6753 1395 1225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7181 276
Size: 53 83 186 936 5647 2468
Fragments arranged by size:
5647 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI NarI
NgoMIV PacI PspOMI PspXI PsrI RsrII SanDI SexAI
SfiI SfoI SrfI SwaI