Restriction map for
pFN21AB4088
(Circular) (Six-base) MAPSORT of: pFN21AB4088.seq Check: 5488 from: 1 to: 6985
pFN21AB4088 6985 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4795 278
Size: 53 83 186 936 3259 2468
Fragments arranged by size:
3259 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2626 3512 4328 1937
Size: 689 886 816 4594
Fragments arranged by size:
4594 886 816 689
Acc65I G'GTAC_C
Cuts at: 3473 3704 4306 3473
Size: 231 602 6152
Fragments arranged by size:
6152 602 231
AclI AA'CG_TT
Cuts at: 1563 5112 5485 1563
Size: 3549 373 3063
Fragments arranged by size:
3549 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 5048 6432 6700 1632
Size: 773 2643 1384 268 1917
Fragments arranged by size:
2643 1917 1384 773 268
AfeI AGC'GCT
Cuts at: 2590 2590
Size: 6985
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6788
AflIII A'CryG_T
Cuts at: 1203 3089 4099 5884 6861 1203
Size: 1886 1010 1785 977 1327
Fragments arranged by size:
1886 1785 1327 1010 977
AgeI A'CCGG_T
Cuts at: 5816 5816
Size: 6985
AhdI GACnn_n'nnGTC
Cuts at: 5714 5714
Size: 6985
AleI CACnn'nnGTG
Cuts at: 1166 2750 1166
Size: 1584 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6985
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 2305
Size: 32 6953
AlwNI CAG_nnn'CTG
Cuts at: 2690 3585 3928 4728 6300 2690
Size: 895 343 800 1572 3375
Fragments arranged by size:
3375 1572 895 800 343
ApaI G_GGCC'C
Cuts at: 3226 3245 3226
Size: 19 6966
ApaLI G'TGCA_C
Cuts at: 5042 6198 5042
Size: 1156 5829
ApoI r'AATT_y
Cuts at: 1493 2452 2528 2864 3159 4294 4520 4672 6781
Size: 959 76 336 295 1135 226 152 2109
Cuts at: 6781 1493
Size: 1697
Fragments arranged by size:
2109 1697 1135 959 336 295 226 152
76
AseI AT'TA_AT
Cuts at: 160 1032 3450 5539 160
Size: 872 2418 2089 1606
Fragments arranged by size:
2418 2089 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
AvaI C'yCGr_G
Cuts at: 1870 1943 4310 1870
Size: 73 2367 4545
Fragments arranged by size:
4545 2367 73
BamHI G'GATC_C
Cuts at: 4315 4682 4315
Size: 367 6618
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2429 3389 3473 3704 4306
Size: 325 217 627 642 960 84 231 602
Cuts at: 4306 5761 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 960 642 627 602 325 231
217 84
BanII G_rGCy'C
Cuts at: 1391 3226 3245 4115 4304 1391
Size: 1835 19 870 189 4072
Fragments arranged by size:
4072 1835 870 189 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4096 928
Size: 971 2197 3817
Fragments arranged by size:
3817 2197 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5176 5210 1903
Size: 34 3239 34 3678
Fragments arranged by size:
3678 3239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4877 6087 3404
Size: 1473 1210 4302
Fragments arranged by size:
4302 1473 1210
BclI T'GATC_A
Cuts at: 1548 2554 2604 1548
Size: 1006 50 5929
Fragments arranged by size:
5929 1006 50
BfrBI ATG'CAT
Cuts at: 3236 3236
Size: 6985
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5596 136
Size: 107 122 71 631 605 3924 1525
Fragments arranged by size:
3924 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6980 6980
Size: 6985
BlpI GC'TnA_GC
Cuts at: 4402 4402
Size: 6985
Bme1580I G_kGCm'C
Cuts at: 1790 3226 3245 5046 6202 1790
Size: 1436 19 1801 1156 2573
Fragments arranged by size:
2573 1801 1436 1156 19
BmgBI CAC'GTC
Cuts at: 1318 2363 1318
Size: 1045 5940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 3595 4716
Size: 416 53 83 399 24 2150 28 1121
Cuts at: 4716 5674 442
Size: 958 1753
Fragments arranged by size:
2150 1753 1121 958 416 399 83 53
28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 3001 3267 3390 4163 5645 1239
Size: 1068 694 266 123 773 1482 2579
Fragments arranged by size:
2579 1482 1068 773 694 266 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5110 5975 6273 5110
Size: 865 298 5822
Fragments arranged by size:
5822 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5648 882
Size: 617 4149 2219
Fragments arranged by size:
4149 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4072 4776 493
Size: 711 2868 704 2702
Fragments arranged by size:
2868 2702 711 704
BsaBI GATnn'nnATC
Cuts at: 2493 6979 2493
Size: 4486 2499
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4792 5174 5809 275
Size: 53 83 186 936 3259 382 635 1451
Fragments arranged by size:
3259 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3260 3550 5418 5816 6090 6237 6648 3260
Size: 290 1868 398 274 147 411 3597
Fragments arranged by size:
3597 1868 411 398 290 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2305 2335 1178
Size: 30 165 30 902 30 5828
Fragments arranged by size:
5828 902 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 1186
Size: 1162 5823
BseYI C'CCAG_C
Cuts at: 3335 3655 6188 6684 3335
Size: 320 2533 496 3636
Fragments arranged by size:
3636 2533 496 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4435 5196 6224 1540
Size: 275 186 2434 761 1028 2301
Fragments arranged by size:
2434 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5046 5131 6202 5046
Size: 85 1071 5829
Fragments arranged by size:
5829 1071 85
BsmI GAATG_Cn'
Cuts at: 2027 3994 4496 4589 2027
Size: 1967 502 93 4423
Fragments arranged by size:
4423 1967 502 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3226 3245 4115 4304 5046 5131 6202
Size: 399 1436 19 870 189 742 85 1071
Cuts at: 6202 1391
Size: 2174
Fragments arranged by size:
2174 1436 1071 870 742 399 189 85
19
BspEI T'CCGG_A
Cuts at: 6648 6648
Size: 6985
BspHI T'CATG_A
Cuts at: 4014 4038 4874 6604 4014
Size: 24 836 1730 4395
Fragments arranged by size:
4395 1730 836 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4340 844
Size: 3496 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4872 1394
Size: 429 3049 3507
Fragments arranged by size:
3507 3049 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3145 5480 5654 65
Size: 1183 1897 2335 174 1396
Fragments arranged by size:
2335 1897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2426 5629 5816 1700
Size: 110 616 3203 187 2869
Fragments arranged by size:
3203 2869 616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6985
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6985
BssSI C'ACGA_G
Cuts at: 2466 3765 3939 5045 6057 2466
Size: 1299 174 1106 1012 3394
Fragments arranged by size:
3394 1299 1106 1012 174
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6985
BstBI TT'CG_AA
Cuts at: 5789 5789
Size: 6985
BstEII G'GTnAC_C
Cuts at: 1184 2921 3100 1184
Size: 1737 179 5069
Fragments arranged by size:
5069 1737 179
BstYI r'GATC_y
Cuts at: 1972 2477 2759 3874 4315 4682 5067 5084 6536
Size: 505 282 1115 441 367 385 17 1452
Cuts at: 6536 6622 6634 6980 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1115 505 441 385 367 346
282 86 17 12
BstZ17I GTA'TAC
Cuts at: 3513 3513
Size: 6985
BtgI C'CryG_G
Cuts at: 513 1065 3711 513
Size: 552 2646 3787
Fragments arranged by size:
3787 2646 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6246
Fragments arranged by size:
6246 683 56
BtsI GCAGTG_nn'
Cuts at: 4501 5295 5315 4501
Size: 794 20 6171
Fragments arranged by size:
6171 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6950
DraI TTT'AAA
Cuts at: 2533 3432 4289 2533
Size: 899 857 5229
Fragments arranged by size:
5229 899 857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4010 5992 809
Size: 507 2694 1982 1802
Fragments arranged by size:
2694 1982 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4432 5321 6950
Size: 54 1425 50 275 18 2602 889 1629
Cuts at: 6950 8
Size: 43
Fragments arranged by size:
2602 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4432 1537
Size: 275 2620 4090
Fragments arranged by size:
4090 2620 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 4915 1353
Size: 1028 2534 3423
Fragments arranged by size:
3423 2534 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5572 5946 6092 387
Size: 1064 138 3983 374 146 1280
Fragments arranged by size:
3983 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2405 3001 3267 3390 4163 5048
Size: 393 675 98 596 266 123 773 885
Cuts at: 5048 5645 6432 6700 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 885 787 773 675 597 596 393
268 266 123 98
EcoO109I rG'GnC_Cy
Cuts at: 3222 3241 3242 3222
Size: 19 1 6965
Fragments arranged by size:
6965 19 1
EcoRI G'AATT_C
Cuts at: 4294 4672 4294
Size: 378 6607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3485 3485
Size: 6985
FspI TGC'GCA
Cuts at: 5491 5491
Size: 6985
HaeII r_GCGC'y
Cuts at: 1678 2592 6132 1678
Size: 914 3540 2531
Fragments arranged by size:
3540 2531 914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 3050 3082 3365
Size: 32 135 32 890 32 556 32 283
Cuts at: 3365 3397 5626 5658 5700 5732 1373
Size: 32 2229 32 42 32 2626
Fragments arranged by size:
2626 2229 890 556 283 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 4329 4575 669
Size: 988 281 940 1451 246 3079
Fragments arranged by size:
3079 1451 988 940 281 246
HindIII A'AGCT_T
Cuts at: 748 2821 748
Size: 2073 4912
HpaI GTT'AAC
Cuts at: 2878 4575 2878
Size: 1697 5288
KpnI G_GTAC'C
Cuts at: 3477 3708 4310 3477
Size: 231 602 6152
Fragments arranged by size:
6152 602 231
MfeI C'AATT_G
Cuts at: 2832 4584 2832
Size: 1752 5233
MluI A'CGCG_T
Cuts at: 6861 6861
Size: 6985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 3341 4120 6099 6283 1305
Size: 1074 962 779 1979 184 2007
Fragments arranged by size:
2007 1979 1074 962 779 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5163
Fragments arranged by size:
5163 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2750 4043 4943 5302 5461 518
Size: 648 1584 1293 900 359 159 2042
Fragments arranged by size:
2042 1584 1293 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3773 4401 5078 6226 6471 1606
Size: 2167 628 677 1148 245 2120
Fragments arranged by size:
2167 2120 1148 677 628 245
NcoI C'CATG_G
Cuts at: 513 1065 3711 513
Size: 552 2646 3787
Fragments arranged by size:
3787 2646 552
NdeI CA'TA_TG
Cuts at: 387 2458 387
Size: 2071 4914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6985
NotI GC'GGCC_GC
Cuts at: 4432 4432
Size: 6985
NsiI A_TGCA'T
Cuts at: 3238 3238
Size: 6985
NspI r_CATG'y
Cuts at: 1132 1229 2906 3093 4103 4343 5888 1132
Size: 97 1677 187 1010 240 1545 2229
Fragments arranged by size:
2229 1677 1545 1010 240 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6985
PciI A'CATG_T
Cuts at: 3089 4099 5884 3089
Size: 1010 1785 4190
Fragments arranged by size:
4190 1785 1010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3399 1108
Size: 2291 4694
PmeI GTTT'AAAC
Cuts at: 4289 4289
Size: 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2337 5054 5086 6567 6599 2305
Size: 32 2717 32 1481 32 2691
Fragments arranged by size:
2717 2691 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6985
PsiI TTA'TAA
Cuts at: 4555 4555
Size: 6985
PspOMI G'GGCC_C
Cuts at: 3222 3241 3222
Size: 19 6966
PstI C_TGCA'G
Cuts at: 830 2359 3031 3778 4337 830
Size: 1529 672 747 559 3478
Fragments arranged by size:
3478 1529 747 672 559
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6985
PvuII CAG'CTG
Cuts at: 3773 3773
Size: 6985
SalI G'TCGA_C
Cuts at: 1936 4327 1936
Size: 2391 4594
SbfI CC_TGCA'GG
Cuts at: 4337 4337
Size: 6985
ScaI AGT'ACT
Cuts at: 5233 6945 5233
Size: 1712 5273
SexAI A'CCwGG_T
Cuts at: 2637 2637
Size: 6985
SfcI C'TryA_G
Cuts at: 826 1045 2127 2355 2763 3027 3774 4333 5468
Size: 219 1082 228 408 264 747 559 1135
Cuts at: 5468 6149 6340 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1082 747 681 559 408 264
228 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6985
SmaI CCC'GGG
Cuts at: 1872 4312 1872
Size: 2440 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5089 5990 6252 820
Size: 197 926 3146 901 262 1553
Fragments arranged by size:
3146 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4072 4776 493
Size: 3579 704 2702
Fragments arranged by size:
3579 2702 704
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6985
SphI G_CATG'C
Cuts at: 1132 4343 1132
Size: 3211 3774
SspI AAT'ATT
Cuts at: 5 52 4909 5
Size: 47 4857 2081
Fragments arranged by size:
4857 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6985
StyI C'CwwG_G
Cuts at: 513 1065 3711 4424 513
Size: 552 2646 713 3074
Fragments arranged by size:
3074 2646 713 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2967 5012 5029 5182 5367 5812 325
Size: 1255 1387 2045 17 153 185 445 1498
Fragments arranged by size:
2045 1498 1387 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5231 6943 96
Size: 274 80 33 51 1443 3254 1712 138
Fragments arranged by size:
3254 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 2925 2950 2957 2982 3676 3708 6945
Size: 32 142 25 7 25 694 32 3237
Cuts at: 6945 6977 2751
Size: 32 2759
Fragments arranged by size:
3237 2759 694 142 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6985
XbaI T'CTAG_A
Cuts at: 4321 4321
Size: 6985
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 2711
Size: 6985
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6985
XmaI C'CCGG_G
Cuts at: 1870 4310 1870
Size: 2440 4545
XmnI GAAnn'nnTTC
Cuts at: 5114 5114
Size: 6985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4793 276
Size: 53 83 186 936 3259 2468
Fragments arranged by size:
3259 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI