Restriction map for
pFN21AB4086
(Circular) (Six-base) MAPSORT of: pFN21AB4086.seq Check: 5215 from: 1 to: 6265
pFN21AB4086 6265 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2144 2709 3608 1937
Size: 207 565 899 4594
Fragments arranged by size:
4594 899 565 207
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2968 3030 3555 4328 5712 5980 1632
Size: 1336 62 525 773 1384 268 1917
Fragments arranged by size:
1917 1384 1336 773 525 268 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 3562 5164 6141 1203
Size: 2359 1602 977 1327
Fragments arranged by size:
2359 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2053 1184
Size: 869 5396
AlwNI CAG_nnn'CTG
Cuts at: 2408 3533 4008 5580 2408
Size: 1125 475 1572 3093
Fragments arranged by size:
3093 1572 1125 475
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3259 3586 5041 618
Size: 325 217 627 1472 327 1455 1842
Fragments arranged by size:
1842 1472 1455 627 327 325 217
BanII G_rGCy'C
Cuts at: 1391 3584 1391
Size: 2193 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 2404 2931 3262 928
Size: 971 425 80 527 331 3931
Fragments arranged by size:
3931 971 527 425 331 80
BbvCI CC'TCA_GC
Cuts at: 3090 3090
Size: 6265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4456 4490 1903
Size: 34 50 34 2435 34 3678
Fragments arranged by size:
3678 2435 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 2098 1548
Size: 550 5715
BfrBI ATG'CAT
Cuts at: 2387 2387
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2073 6260 2073
Size: 4187 2078
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 4326 5482 1790
Size: 2536 1156 2573
Fragments arranged by size:
2573 2536 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2725 3996 4954
Size: 416 53 83 399 24 1308 1271 958
Cuts at: 4954 442
Size: 1753
Fragments arranged by size:
1753 1308 1271 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2973 1056
Size: 1917 4348
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4925 1239
Size: 3686 2579
Bpu10I CC'TnA_GC
Cuts at: 3090 3090
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 4390 5255 5553 2090
Size: 2300 865 298 2802
Fragments arranged by size:
2802 2300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 4072 4454 5089
Size: 53 83 186 936 1876 663 382 635
Cuts at: 5089 275
Size: 1451
Fragments arranged by size:
1876 1451 936 663 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3495 3525 1178
Size: 30 165 30 2092 30 3918
Fragments arranged by size:
3918 2092 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6265
BseYI C'CCAG_C
Cuts at: 5468 5964 5468
Size: 496 5769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2164 1163
Size: 573 428 5264
Fragments arranged by size:
5264 573 428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4326 4411 5482 4326
Size: 85 1071 5109
Fragments arranged by size:
5109 1071 85
BsmI GAATG_Cn'
Cuts at: 2032 3457 3776 3869 2032
Size: 1425 319 93 4428
Fragments arranged by size:
4428 1425 319 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3584 4326 4411 5482 1391
Size: 399 1794 742 85 1071 2174
Fragments arranged by size:
2174 1794 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 2784 4154 5884 2784
Size: 1370 1730 3165
Fragments arranged by size:
3165 1730 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3036 96
Size: 2940 3325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2155
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2073 2721 3595 3962 4347 4364 5816 5902
Size: 101 648 874 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 874 648 385 367 346 101
86 17 12
BstZ17I GTA'TAC
Cuts at: 2145 2145
Size: 6265
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2034 2069 529
Size: 35 1470 35 4725
Fragments arranged by size:
4725 1470 35 35
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 3712 4601
Size: 54 1425 50 275 18 212 1670 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1670 1629 1425 889 275 212 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2987 3101 4195 1353
Size: 984 650 114 1094 3423
Fragments arranged by size:
3423 1094 984 650 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2968 3030 3555 4328 4925 5712 5980
Size: 393 1336 62 525 773 597 787 268
Cuts at: 5980 1239
Size: 1524
Fragments arranged by size:
1524 1336 787 773 597 525 393 268
62
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2618 2650 3215
Size: 32 135 32 614 32 400 32 565
Cuts at: 3215 3247 4906 4938 4980 5012 1373
Size: 32 1659 32 42 32 2626
Fragments arranged by size:
2626 1659 614 565 400 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3316 3609 3855 669
Size: 988 281 487 891 293 246 3079
Fragments arranged by size:
3079 988 891 487 293 281 246
HindIII A'AGCT_T
Cuts at: 748 2034 748
Size: 1286 4979
HpaI GTT'AAC
Cuts at: 2425 3855 2425
Size: 1430 4835
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2260 2506 5379 5563 1305
Size: 786 169 246 2873 184 2007
Fragments arranged by size:
2873 2007 786 246 184 169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2044 10
Size: 54 1425 343 212 4231
Fragments arranged by size:
4231 1425 343 212 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2607 4223 4582 4741 518
Size: 648 1050 391 1616 359 159 2042
Fragments arranged by size:
2042 1616 1050 648 391 359 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2405 2697 3542 3681 4358 5506 5751
Size: 729 70 292 845 139 677 1148 245
Cuts at: 5751 1606
Size: 2120
Fragments arranged by size:
2120 1148 845 729 677 292 245 139
70
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5713
NdeI CA'TA_TG
Cuts at: 387 2595 387
Size: 2208 4057
NheI G'CTAG_C
Cuts at: 1052 2969 1052
Size: 1917 4348
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NsiI A_TGCA'T
Cuts at: 2389 2389
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2391 2606 3566 3623 5168 1132
Size: 97 1162 215 960 57 1545 2229
Fragments arranged by size:
2229 1545 1162 960 215 97 57
PasI CC'CwG_GG
Cuts at: 1348 3328 1348
Size: 1980 4285
PciI A'CATG_T
Cuts at: 3562 5164 3562
Size: 1602 4663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2445 3310 1108
Size: 1337 865 4063
Fragments arranged by size:
4063 1337 865
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3388 3420 4334 4366 5847 5879 3388
Size: 32 914 32 1481 32 3774
Fragments arranged by size:
3774 1481 914 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3617 830
Size: 2787 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2335 2405 3542 2335
Size: 70 1137 5058
Fragments arranged by size:
5058 1137 70
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 3083 4513 6225 3083
Size: 1430 1712 3123
Fragments arranged by size:
3123 1712 1430
SfcI C'TryA_G
Cuts at: 826 1045 2514 2646 2887 3613 4748 5429 5620
Size: 219 1469 132 241 726 1135 681 191
Cuts at: 5620 826
Size: 1471
Fragments arranged by size:
1471 1469 1135 726 681 241 219 191
132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 4369 5270 5532 820
Size: 197 926 126 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197 126
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 2391 3623 1132
Size: 1259 1232 3774
Fragments arranged by size:
3774 1259 1232
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 3704 513
Size: 552 2639 3074
Fragments arranged by size:
3074 2639 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 4292 4309 4462 4647 5092 325
Size: 1255 1737 975 17 153 185 445 1498
Fragments arranged by size:
1737 1498 1255 975 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3036 3081 3153
Size: 274 80 33 51 1443 1059 45 72
Cuts at: 3153 4511 6223 96
Size: 1358 1712 138
Fragments arranged by size:
1712 1443 1358 1059 274 138 80 72
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 2849 4394 2849
Size: 1545 4720
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI