Restriction map for
pFN21AB4084
(Circular) (Six-base) MAPSORT of: pFN21AB4084.seq Check: 5630 from: 1 to: 7612
pFN21AB4084 7612 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4828 4828
Size: 7612
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5422 278
Size: 53 83 186 936 3886 2468
Fragments arranged by size:
3886 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4955 1937
Size: 3018 4594
Acc65I G'GTAC_C
Cuts at: 4933 4933
Size: 7612
AclI AA'CG_TT
Cuts at: 1563 5739 6112 1563
Size: 4176 373 3063
Fragments arranged by size:
4176 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 3435 4139 4480 5675 7059 7327 1632
Size: 1379 424 704 341 1195 1384 268 1917
Fragments arranged by size:
1917 1384 1379 1195 704 424 341 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7415
AflIII A'CryG_T
Cuts at: 1203 2448 3280 4041 6511 7488 1203
Size: 1245 832 761 2470 977 1327
Fragments arranged by size:
2470 1327 1245 977 832 761
AgeI A'CCGG_T
Cuts at: 6443 6443
Size: 7612
AhdI GACnn_n'nnGTC
Cuts at: 2290 3542 6341 2290
Size: 1252 2799 3561
Fragments arranged by size:
3561 2799 1252
AleI CACnn'nnGTG
Cuts at: 1166 4785 1166
Size: 3619 3993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2469 2963 1184
Size: 1285 494 5833
Fragments arranged by size:
5833 1285 494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3270 3302 3270
Size: 32 7580
AlwNI CAG_nnn'CTG
Cuts at: 2129 2872 5355 6927 2129
Size: 743 2483 1572 2814
Fragments arranged by size:
2814 2483 1572 743
ApaI G_GGCC'C
Cuts at: 2821 4809 2821
Size: 1988 5624
ApaLI G'TGCA_C
Cuts at: 2085 3625 4220 4397 5669 6825 2085
Size: 1540 595 177 1272 1156 2872
Fragments arranged by size:
2872 1540 1272 1156 595 177
ApoI r'AATT_y
Cuts at: 1493 3034 4076 4202 4528 4921 5147 5299 7408
Size: 1541 1042 126 326 393 226 152 2109
Cuts at: 7408 1493
Size: 1697
Fragments arranged by size:
2109 1697 1541 1042 393 326 226 152
126
AseI AT'TA_AT
Cuts at: 160 1032 6166 160
Size: 872 5134 1606
Fragments arranged by size:
5134 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7612
AvaI C'yCGr_G
Cuts at: 1870 1943 2705 2747 4551 4937 1870
Size: 73 762 42 1804 386 4545
Fragments arranged by size:
4545 1804 762 386 73 42
AvrII C'CTAG_G
Cuts at: 2258 2258
Size: 7612
BamHI G'GATC_C
Cuts at: 2701 4146 4942 5309 2701
Size: 1445 796 367 5004
Fragments arranged by size:
5004 1445 796 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2229 4933 6388 618
Size: 325 217 627 442 2704 1455 1842
Fragments arranged by size:
2704 1842 1455 627 442 325 217
BanII G_rGCy'C
Cuts at: 1391 2120 2821 4809 4931 1391
Size: 729 701 1988 122 4072
Fragments arranged by size:
4072 1988 729 701 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2478 3194 3277 3859 4900 928
Size: 971 579 716 83 582 1041 3640
Fragments arranged by size:
3640 1041 971 716 582 579 83
BbvCI CC'TCA_GC
Cuts at: 2754 2754
Size: 7612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2312 2346 3869 3903 5803 5837 1903
Size: 34 375 34 1523 34 1900 34 3678
Fragments arranged by size:
3678 1900 1523 375 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4083 5504 6714 4083
Size: 1421 1210 4981
Fragments arranged by size:
4981 1421 1210
BclI T'GATC_A
Cuts at: 1548 3502 1548
Size: 1954 5658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2825 6223 136
Size: 107 122 71 631 605 1153 3398 1525
Fragments arranged by size:
3398 1525 1153 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3810 7607 3810
Size: 3797 3815
BlpI GC'TnA_GC
Cuts at: 3680 5029 3680
Size: 1349 6263
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2821 3336 3629 3855 4224 4394 4401
Size: 299 732 515 293 226 369 170 7
Cuts at: 4401 4468 4809 5673 6829 1790
Size: 67 341 864 1156 2573
Fragments arranged by size:
2573 1156 864 732 515 369 341 299
293 226 170 67 7
BmgBI CAC'GTC
Cuts at: 1318 2341 3865 1318
Size: 1023 1524 5065
Fragments arranged by size:
5065 1524 1023
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3192 5343 6301
Size: 416 53 83 399 24 1775 2151 958
Cuts at: 6301 442
Size: 1753
Fragments arranged by size:
2151 1775 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2830 3134 4436 6272 1239
Size: 886 705 304 1302 1836 2579
Fragments arranged by size:
2579 1836 1302 886 705 304
Bpu10I CC'TnA_GC
Cuts at: 2489 2754 2489
Size: 265 7347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5737 6602 6900 5737
Size: 865 298 6449
Fragments arranged by size:
6449 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2884 2921 3228 6275 882
Size: 617 1385 37 307 3047 2219
Fragments arranged by size:
3047 2219 1385 617 307 37
BsaAI yAC'GTr
Cuts at: 493 1204 2449 3508 5403 493
Size: 711 1245 1059 1895 2702
Fragments arranged by size:
2702 1895 1245 1059 711
BsaBI GATnn'nnATC
Cuts at: 2981 7606 2981
Size: 4625 2987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3708 5419 5801 6436
Size: 53 83 186 936 2175 1711 382 635
Cuts at: 6436 275
Size: 1451
Fragments arranged by size:
2175 1711 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2886 6045 6443 6717 6864 7275 2886
Size: 3159 398 274 147 411 3223
Fragments arranged by size:
3223 3159 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7387
Fragments arranged by size:
7387 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 2831 3080 1186
Size: 1546 99 249 5718
Fragments arranged by size:
5718 1546 249 99
BseYI C'CCAG_C
Cuts at: 2333 2894 3290 3616 4482 4607 6815 7311 2333
Size: 561 396 326 866 125 2208 496 2634
Fragments arranged by size:
2634 2208 866 561 496 396 326 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2127 3025 3721 1163
Size: 573 391 898 696 5054
Fragments arranged by size:
5054 898 696 573 391
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5062 5823 6851 1540
Size: 275 186 3061 761 1028 2301
Fragments arranged by size:
3061 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2089 2690 2841 3629 4224 4401 5673 5758 6829
Size: 601 151 788 595 177 1272 85 1071
Cuts at: 6829 2089
Size: 2872
Fragments arranged by size:
2872 1272 1071 788 601 595 177 151
85
BsiWI C'GTAC_G
Cuts at: 4057 4057
Size: 7612
BsmI GAATG_Cn'
Cuts at: 5123 5216 5123
Size: 93 7519
BsmBI CGTCTCn'nnnn_
Cuts at: 2298 4571 2298
Size: 2273 5339
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2120 2690 2821 2841 3336 3629
Size: 399 299 31 570 131 20 495 293
Cuts at: 3629 3855 4224 4394 4401 4468 4809 4931 5673
Size: 226 369 170 7 67 341 122 742
Cuts at: 5673 5758 6829 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 495 399 369 341
299 293 226 170 131 122 85 67
31 20 7
BspEI T'CCGG_A
Cuts at: 2886 7275 2886
Size: 4389 3223
BspHI T'CATG_A
Cuts at: 3652 3750 4572 5501 7231 3652
Size: 98 822 929 1730 4033
Fragments arranged by size:
4033 1730 929 822 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3222 4485 4828 4967 844
Size: 2378 1263 343 139 3489
Fragments arranged by size:
3489 2378 1263 343 139
BsrBI CCG'CTC
Cuts at: 1394 1823 5499 1394
Size: 429 3676 3507
Fragments arranged by size:
3676 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6107 6281 65
Size: 1183 4859 174 1396
Fragments arranged by size:
4859 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 3176 6256 6443 1700
Size: 110 386 980 3080 187 2869
Fragments arranged by size:
3080 2869 980 386 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7612
BssHII G'CGCG_C
Cuts at: 1925 4843 1925
Size: 2918 4694
BssSI C'ACGA_G
Cuts at: 5672 6684 5672
Size: 1012 6600
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4477 794
Size: 3683 3929
BstBI TT'CG_AA
Cuts at: 6416 6416
Size: 7612
BstEII G'GTnAC_C
Cuts at: 1184 2905 1184
Size: 1721 5891
BstXI CCAn_nnnn'nTGG
Cuts at: 2333 3078 3718 3983 4414 4585 2333
Size: 745 640 265 431 171 5360
Fragments arranged by size:
5360 745 640 431 265 171
BstYI r'GATC_y
Cuts at: 1972 2701 3810 4146 4942 5309 5694 5711 7163
Size: 729 1109 336 796 367 385 17 1452
Cuts at: 7163 7249 7261 7607 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1109 796 729 385 367 346
336 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2094 2441 2094
Size: 347 7265
BtgI C'CryG_G
Cuts at: 513 1065 2004 2225 2901 3321 4848 513
Size: 552 939 221 676 420 1527 3277
Fragments arranged by size:
3277 1527 939 676 552 420 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3247 487
Size: 683 56 2021 4852
Fragments arranged by size:
4852 2021 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 2110 2857 5128 5922 5942 2087
Size: 23 747 2271 794 20 3757
Fragments arranged by size:
3757 2271 794 747 23 20
ClaI AT'CG_AT
Cuts at: 1554 2563 1554
Size: 1009 6603
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7577
DraI TTT'AAA
Cuts at: 4916 4916
Size: 7612
DraIII CAC_nnn'GTG
Cuts at: 2685 2685
Size: 7612
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6619 809
Size: 507 5303 1802
Fragments arranged by size:
5303 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2026 3080
Size: 54 1425 50 275 18 177 19 1054
Cuts at: 3080 5059 5948 7577 8
Size: 1979 889 1629 43
Fragments arranged by size:
1979 1629 1425 1054 889 275 177 54
50 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 5059 1537
Size: 275 3247 4090
Fragments arranged by size:
4090 3247 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 2574 2882 3380 3483 3627 4904 5542
Size: 810 411 308 498 103 144 1277 638
Cuts at: 5542 1353
Size: 3423
Fragments arranged by size:
3423 1277 810 638 498 411 308 144
103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3358 6199 6573 6719 387
Size: 1064 138 1769 2841 374 146 1280
Fragments arranged by size:
2841 1769 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2830 3011 3134 3435 4139 4436
Size: 393 493 705 181 123 301 704 297
Cuts at: 4436 4480 5675 6272 7059 7327 1239
Size: 44 1195 597 787 268 1524
Fragments arranged by size:
1524 1195 787 705 704 597 493 393
301 297 268 181 123 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2097 2747 2097
Size: 650 6962
EcoO109I rG'GnC_Cy
Cuts at: 2345 2385 2437 2818 3796 4806 4891 2345
Size: 40 52 381 978 1010 85 5066
Fragments arranged by size:
5066 1010 978 381 85 52 40
EcoRI G'AATT_C
Cuts at: 3034 4076 4921 5299 3034
Size: 1042 845 378 5347
Fragments arranged by size:
5347 1042 845 378
FspI TGC'GCA
Cuts at: 6118 6118
Size: 7612
HaeII r_GCGC'y
Cuts at: 1678 6759 1678
Size: 5081 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2686 2718 3056
Size: 32 135 32 704 32 378 32 338
Cuts at: 3056 3088 3115 3130 3147 3162 3206 3238 3487
Size: 32 27 15 17 15 44 32 249
Cuts at: 3487 3519 3528 3560 3909 3941 6253 6285 6327
Size: 32 9 32 349 32 2312 32 42
Cuts at: 6327 6359 1373
Size: 32 2626
Fragments arranged by size:
2626 2312 704 378 349 338 249 135
44 42 32 32 32 32 32 32
32 32 32 32 32 27 17 15
15 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2809 3384 3400 3835 4705 4956
Size: 988 281 871 575 16 435 870 251
Cuts at: 4956 5202 669
Size: 246 3079
Fragments arranged by size:
3079 988 871 870 575 435 281 251
246 16
HindIII A'AGCT_T
Cuts at: 748 3443 748
Size: 2695 4917
HpaI GTT'AAC
Cuts at: 3384 5202 3384
Size: 1818 5794
KpnI G_GTAC'C
Cuts at: 4937 4937
Size: 7612
MfeI C'AATT_G
Cuts at: 5211 5211
Size: 7612
MluI A'CGCG_T
Cuts at: 3280 7488 3280
Size: 4208 3404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3124 3946 6726 6910 1305
Size: 1819 822 2780 184 2007
Fragments arranged by size:
2780 2007 1819 822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2028 3082 10
Size: 54 1425 343 177 19 1054 4540
Fragments arranged by size:
4540 1425 1054 343 177 54 19
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 3716 3981 4785 5570 5929 6088
Size: 648 1779 771 265 804 785 359 159
Cuts at: 6088 518
Size: 2042
Fragments arranged by size:
2042 1779 804 785 771 648 359 265
159
MspA1I CmG'CkG
Cuts at: 1606 2037 2082 2533 3121 3679 4262 5028 5705
Size: 431 45 451 588 558 583 766 677
Cuts at: 5705 6853 7098 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 588 583 558 451
431 245 45
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 7612
NcoI C'CATG_G
Cuts at: 513 1065 2004 3321 4848 513
Size: 552 939 1317 1527 3277
Fragments arranged by size:
3277 1527 1317 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7612
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 7612
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7612
NotI GC'GGCC_GC
Cuts at: 5059 5059
Size: 7612
NspI r_CATG'y
Cuts at: 1132 1229 3610 4436 4970 6515 1132
Size: 97 2381 826 534 1545 2229
Fragments arranged by size:
2381 2229 1545 826 534 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7612
PciI A'CATG_T
Cuts at: 6511 6511
Size: 7612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2199 3322 1108
Size: 1091 1123 5398
Fragments arranged by size:
5398 1123 1091
PfoI T'CCnGG_A
Cuts at: 2141 4025 2141
Size: 1884 5728
PmeI GTTT'AAAC
Cuts at: 4916 4916
Size: 7612
PmlI CAC'GTG
Cuts at: 2449 3508 2449
Size: 1059 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5681 5713 7194 7226 5681
Size: 32 1481 32 6067
Fragments arranged by size:
6067 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2345 2385 2437 3796 2345
Size: 40 52 1359 6161
Fragments arranged by size:
6161 1359 52 40
PshAI GACnn'nnGTC
Cuts at: 1613 2037 2343 1613
Size: 424 306 6882
Fragments arranged by size:
6882 424 306
PsiI TTA'TAA
Cuts at: 5182 5182
Size: 7612
PspOMI G'GGCC_C
Cuts at: 2817 4805 2817
Size: 1988 5624
PspXI vC'TCGA_Gb
Cuts at: 4551 4551
Size: 7612
PstI C_TGCA'G
Cuts at: 830 2074 4964 830
Size: 1244 2890 3478
Fragments arranged by size:
3478 2890 1244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7612
PvuII CAG'CTG
Cuts at: 2037 2082 3121 3679 2037
Size: 45 1039 558 5970
Fragments arranged by size:
5970 1039 558 45
SalI G'TCGA_C
Cuts at: 1936 4954 1936
Size: 3018 4594
SanDI GG'GwC_CC
Cuts at: 3796 3796
Size: 7612
SapI GCTCTTCn'nnn_
Cuts at: 2574 2882 3483 4904 2574
Size: 308 601 1421 5282
Fragments arranged by size:
5282 1421 601 308
SbfI CC_TGCA'GG
Cuts at: 4964 4964
Size: 7612
ScaI AGT'ACT
Cuts at: 4616 5860 7572 4616
Size: 1244 1712 4656
Fragments arranged by size:
4656 1712 1244
SfcI C'TryA_G
Cuts at: 826 1045 2070 4741 4960 6095 6776 6967 826
Size: 219 1025 2671 219 1135 681 191 1471
Fragments arranged by size:
2671 1471 1135 1025 681 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7612
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7612
SmaI CCC'GGG
Cuts at: 1872 4939 1872
Size: 3067 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4551 5716 6617 6879 820
Size: 197 926 2608 1165 901 262 1553
Fragments arranged by size:
2608 1553 1165 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5403 493
Size: 4910 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7612
SphI G_CATG'C
Cuts at: 1132 4970 1132
Size: 3838 3774
SspI AAT'ATT
Cuts at: 5 52 5536 5
Size: 47 5484 2081
Fragments arranged by size:
5484 2081 47
StuI AGG'CCT
Cuts at: 1517 2257 4413 1517
Size: 740 2156 4716
Fragments arranged by size:
4716 2156 740
StyI C'CwwG_G
Cuts at: 513 1065 2004 2258 2543 3321 3532 4848 5051
Size: 552 939 254 285 778 211 1316 203
Cuts at: 5051 513
Size: 3074
Fragments arranged by size:
3074 1316 939 778 552 285 254 211
203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 4207 5639 5656 5809 5994 6439
Size: 1255 2295 332 1432 17 153 185 445
Cuts at: 6439 325
Size: 1498
Fragments arranged by size:
2295 1498 1432 1255 445 332 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2934 4380 4614
Size: 274 80 33 51 1443 957 1446 234
Cuts at: 4614 5858 7570 96
Size: 1244 1712 138
Fragments arranged by size:
1712 1446 1443 1244 957 274 234 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7572 7604 7572
Size: 32 7580
Tth111I GACn'n_nGTC
Cuts at: 1316 4709 1316
Size: 3393 4219
XbaI T'CTAG_A
Cuts at: 3460 4948 3460
Size: 1488 6124
XhoI C'TCGA_G
Cuts at: 1943 4551 1943
Size: 2608 5004
XmaI C'CCGG_G
Cuts at: 1870 4937 1870
Size: 3067 4545
XmnI GAAnn'nnTTC
Cuts at: 3374 5741 3374
Size: 2367 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5420 276
Size: 53 83 186 936 3886 2468
Fragments arranged by size:
3886 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BplI BstZ17I EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI XcmI