Restriction map for
pFN21AB4076
(Circular) (Six-base) MAPSORT of: pFN21AB4076.seq Check: 7009 from: 1 to: 6424
pFN21AB4076 6424 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4234 278
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3767 1937
Size: 1830 4594
Acc65I G'GTAC_C
Cuts at: 3745 3745
Size: 6424
AclI AA'CG_TT
Cuts at: 1563 4551 4924 1563
Size: 2988 373 3063
Fragments arranged by size:
3063 2988 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2809 2977 3565 4487 5871 6139 1632
Size: 1177 168 588 922 1384 268 1917
Fragments arranged by size:
1917 1384 1177 922 588 268 168
AfeI AGC'GCT
Cuts at: 3663 3663
Size: 6424
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6227
AflIII A'CryG_T
Cuts at: 1203 3053 5323 6300 1203
Size: 1850 2270 977 1327
Fragments arranged by size:
2270 1850 1327 977
AgeI A'CCGG_T
Cuts at: 5255 5255
Size: 6424
AhdI GACnn_n'nnGTC
Cuts at: 5153 5153
Size: 6424
AleI CACnn'nnGTG
Cuts at: 1166 2703 1166
Size: 1537 4887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2141
Size: 32 6392
AlwNI CAG_nnn'CTG
Cuts at: 2270 4167 5739 2270
Size: 1897 1572 2955
Fragments arranged by size:
2955 1897 1572
ApaLI G'TGCA_C
Cuts at: 4481 5637 4481
Size: 1156 5268
ApoI r'AATT_y
Cuts at: 1493 2238 2819 3279 3531 3733 3959 4111 6220
Size: 745 581 460 252 202 226 152 2109
Cuts at: 6220 1493
Size: 1697
Fragments arranged by size:
2109 1697 745 581 460 252 226 202
152
AseI AT'TA_AT
Cuts at: 160 1032 4978 160
Size: 872 3946 1606
Fragments arranged by size:
3946 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
AvaI C'yCGr_G
Cuts at: 1870 1943 3749 1870
Size: 73 1806 4545
Fragments arranged by size:
4545 1806 73
BamHI G'GATC_C
Cuts at: 3754 4121 3754
Size: 367 6057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3745 5200 618
Size: 325 217 627 1958 1455 1842
Fragments arranged by size:
1958 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2220 3743 1391
Size: 829 1523 4072
Fragments arranged by size:
4072 1523 829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 928
Size: 971 617 4836
Fragments arranged by size:
4836 971 617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4615 4649 1903
Size: 34 2678 34 3678
Fragments arranged by size:
3678 2678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3441 3683 4316 5526 3441
Size: 242 633 1210 4339
Fragments arranged by size:
4339 1210 633 242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5035 136
Size: 107 122 71 631 605 3363 1525
Fragments arranged by size:
3363 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3254 6419 3254
Size: 3165 3259
BlpI GC'TnA_GC
Cuts at: 2106 2535 3841 2106
Size: 429 1306 4689
Fragments arranged by size:
4689 1306 429
Bme1580I G_kGCm'C
Cuts at: 1790 4485 5641 1790
Size: 2695 1156 2573
Fragments arranged by size:
2695 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6424
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4155 5113 442
Size: 416 53 83 399 24 2738 958 1753
Fragments arranged by size:
2738 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 6424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2889 3141 3393 3477 3645 5084 1239
Size: 917 733 252 252 84 168 1439 2579
Fragments arranged by size:
2579 1439 917 733 252 252 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4549 5414 5712 4549
Size: 865 298 5261
Fragments arranged by size:
5261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 2397 3542 5087 882
Size: 617 744 154 1145 1545 2219
Fragments arranged by size:
2219 1545 1145 744 617 154
BsaAI yAC'GTr
Cuts at: 493 1204 4215 493
Size: 711 3011 2702
Fragments arranged by size:
3011 2702 711
BsaBI GATnn'nnATC
Cuts at: 6418 6418
Size: 6424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4231 4613 5248 275
Size: 53 83 186 936 2698 382 635 1451
Fragments arranged by size:
2698 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2196 3501 4857 5255 5529 5676 6087 2196
Size: 1305 1356 398 274 147 411 2533
Fragments arranged by size:
2533 1356 1305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6199
Fragments arranged by size:
6199 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5514
BseYI C'CCAG_C
Cuts at: 5627 6123 5627
Size: 496 5928
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5851
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3874 4635 5663 1540
Size: 275 186 1873 761 1028 2301
Fragments arranged by size:
2301 1873 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3173 4485 4570 5641 3173
Size: 1312 85 1071 3956
Fragments arranged by size:
3956 1312 1071 85
BsmI GAATG_Cn'
Cuts at: 2456 3227 3479 3935 4028 2456
Size: 771 252 456 93 4852
Fragments arranged by size:
4852 771 456 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 3173 3743 4485 4570 5641 1391
Size: 399 430 953 570 742 85 1071 2174
Fragments arranged by size:
2174 1071 953 742 570 430 399 85
BspEI T'CCGG_A
Cuts at: 3501 6087 3501
Size: 2586 3838
BspHI T'CATG_A
Cuts at: 3394 4313 6043 3394
Size: 919 1730 3775
Fragments arranged by size:
3775 1730 919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3779 844
Size: 2935 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4311 1394
Size: 429 2488 3507
Fragments arranged by size:
3507 2488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3232 4919 5093 65
Size: 1183 1984 1687 174 1396
Fragments arranged by size:
1984 1687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5068 5255 1700
Size: 110 3258 187 2869
Fragments arranged by size:
3258 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2118 3048 96
Size: 2022 930 3472
Fragments arranged by size:
3472 2022 930
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6424
BssSI C'ACGA_G
Cuts at: 4484 5496 4484
Size: 1012 5412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6424
BstBI TT'CG_AA
Cuts at: 5228 5228
Size: 6424
BstEII G'GTnAC_C
Cuts at: 1184 2044 1184
Size: 860 5564
BstYI r'GATC_y
Cuts at: 1972 3254 3754 4121 4506 4523 5975 6061 6073
Size: 1282 500 367 385 17 1452 86 12
Cuts at: 6073 6419 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1282 500 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4933
Fragments arranged by size:
4933 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5685
Fragments arranged by size:
5685 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3940 4734 4754 2100
Size: 1840 794 20 3770
Fragments arranged by size:
3770 1840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3214 3249 3634 3669 529
Size: 35 2650 35 385 35 3284
Fragments arranged by size:
3284 2650 385 35 35 35
DraI TTT'AAA
Cuts at: 2314 2589 3728 2314
Size: 275 1139 5010
Fragments arranged by size:
5010 1139 275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5431 809
Size: 507 4115 1802
Fragments arranged by size:
4115 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3871 4760 6389
Size: 54 1425 50 275 18 2041 889 1629
Cuts at: 6389 8
Size: 43
Fragments arranged by size:
2041 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3871 1537
Size: 275 2059 4090
Fragments arranged by size:
4090 2059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2208 3540 4354 1353
Size: 855 1332 814 3423
Fragments arranged by size:
3423 1332 855 814
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5011 5385 5531 387
Size: 1064 138 3422 374 146 1280
Fragments arranged by size:
3422 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2809 2889 2977 3141 3393 3477
Size: 393 524 653 80 88 164 252 84
Cuts at: 3477 3565 3645 4487 5084 5871 6139 1239
Size: 88 80 842 597 787 268 1524
Fragments arranged by size:
1524 842 787 653 597 524 393 268
252 164 88 88 84 80 80
EcoO109I rG'GnC_Cy
Cuts at: 3213 3213
Size: 6424
EcoRI G'AATT_C
Cuts at: 3279 3531 3733 4111 3279
Size: 252 202 378 5592
Fragments arranged by size:
5592 378 252 202
FspI TGC'GCA
Cuts at: 4930 4930
Size: 6424
HaeII r_GCGC'y
Cuts at: 1678 2836 3665 5571 1678
Size: 1158 829 1906 2531
Fragments arranged by size:
2531 1906 1158 829
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2309 2341 3160
Size: 32 135 32 428 32 277 32 819
Cuts at: 3160 3192 5065 5097 5139 5171 1373
Size: 32 1873 32 42 32 2626
Fragments arranged by size:
2626 1873 819 428 277 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3768 4014 669
Size: 988 281 1830 246 3079
Fragments arranged by size:
3079 1830 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6424
HpaI GTT'AAC
Cuts at: 4014 4014
Size: 6424
KpnI G_GTAC'C
Cuts at: 3749 3749
Size: 6424
MfeI C'AATT_G
Cuts at: 4023 4023
Size: 6424
MluI A'CGCG_T
Cuts at: 6300 6300
Size: 6424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 3520 5538 5722 1305
Size: 876 1339 2018 184 2007
Fragments arranged by size:
2018 2007 1339 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4602
Fragments arranged by size:
4602 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 2565 2703 2952 3388 3399 3472
Size: 648 1173 226 138 249 436 11 73
Cuts at: 3472 4382 4741 4900 518
Size: 910 359 159 2042
Fragments arranged by size:
2042 1173 910 648 436 359 249 226
159 138 73 11
MspA1I CmG'CkG
Cuts at: 1606 2829 2997 3840 4517 5665 5910 1606
Size: 1223 168 843 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 843 677 245 168
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4933
Fragments arranged by size:
4933 939 552
NdeI CA'TA_TG
Cuts at: 387 3385 3469 387
Size: 2998 84 3342
Fragments arranged by size:
3342 2998 84
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6424
NotI GC'GGCC_GC
Cuts at: 3871 3871
Size: 6424
NspI r_CATG'y
Cuts at: 1132 1229 3057 3645 3782 5327 1132
Size: 97 1828 588 137 1545 2229
Fragments arranged by size:
2229 1828 1545 588 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6424
PciI A'CATG_T
Cuts at: 3053 5323 3053
Size: 2270 4154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6424
PfoI T'CCnGG_A
Cuts at: 2033 2033
Size: 6424
PmeI GTTT'AAAC
Cuts at: 3728 3728
Size: 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 6006 6038 4493
Size: 32 1481 32 4879
Fragments arranged by size:
4879 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6424
PsiI TTA'TAA
Cuts at: 3994 3994
Size: 6424
PstI C_TGCA'G
Cuts at: 830 3776 830
Size: 2946 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6424
PvuII CAG'CTG
Cuts at: 2829 2997 2829
Size: 168 6256
SalI G'TCGA_C
Cuts at: 1936 3766 1936
Size: 1830 4594
SapI GCTCTTCn'nnn_
Cuts at: 2208 2208
Size: 6424
SbfI CC_TGCA'GG
Cuts at: 3776 3776
Size: 6424
ScaI AGT'ACT
Cuts at: 2475 2544 4672 6384 2475
Size: 69 2128 1712 2515
Fragments arranged by size:
2515 2128 1712 69
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 6424
SfcI C'TryA_G
Cuts at: 826 1045 3772 4907 5588 5779 826
Size: 219 2727 1135 681 191 1471
Fragments arranged by size:
2727 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6424
SmaI CCC'GGG
Cuts at: 1872 3751 1872
Size: 1879 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4528 5429 5691 820
Size: 197 926 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4215 493
Size: 3722 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6424
SphI G_CATG'C
Cuts at: 1132 3782 1132
Size: 2650 3774
SspI AAT'ATT
Cuts at: 5 52 4348 5
Size: 47 4296 2081
Fragments arranged by size:
4296 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6424
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 3863 513
Size: 552 939 156 1703 3074
Fragments arranged by size:
3074 1703 939 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 4451 4468 4621 4806 5251 325
Size: 1255 897 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2473 2542
Size: 274 80 33 51 1443 141 355 69
Cuts at: 2542 3048 4670 6382 96
Size: 506 1622 1712 138
Fragments arranged by size:
1712 1622 1443 506 355 274 141 138
80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6384 6416 6384
Size: 32 6392
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6424
XbaI T'CTAG_A
Cuts at: 3760 3760
Size: 6424
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6424
XmaI C'CCGG_G
Cuts at: 1870 3749 1870
Size: 1879 4545
XmnI GAAnn'nnTTC
Cuts at: 3158 3494 4553 3158
Size: 336 1059 5029
Fragments arranged by size:
5029 1059 336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4232 276
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI