Restriction map for
pFN21AB4058
(Circular) (Six-base) MAPSORT of: pFN21AB4058.seq Check: 3974 from: 1 to: 6316
pFN21AB4058 6316 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4126 278
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3659 1937
Size: 1722 4594
Acc65I G'GTAC_C
Cuts at: 3637 3637
Size: 6316
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2553 4379 5763 6031 1632
Size: 921 1826 1384 268 1917
Fragments arranged by size:
1917 1826 1384 921 268
AflII C'TTAA_G
Cuts at: 820 1017 3518 820
Size: 197 2501 3618
Fragments arranged by size:
3618 2501 197
AflIII A'CryG_T
Cuts at: 1203 5215 6192 1203
Size: 4012 977 1327
Fragments arranged by size:
4012 1327 977
AgeI A'CCGG_T
Cuts at: 5147 5147
Size: 6316
AhdI GACnn_n'nnGTC
Cuts at: 5045 5045
Size: 6316
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6316
AlwNI CAG_nnn'CTG
Cuts at: 4059 5631 4059
Size: 1572 4744
ApaI G_GGCC'C
Cuts at: 2359 2359
Size: 6316
ApaLI G'TGCA_C
Cuts at: 3212 4373 5529 3212
Size: 1161 1156 3999
Fragments arranged by size:
3999 1161 1156
ApoI r'AATT_y
Cuts at: 1493 3625 3851 4003 6112 1493
Size: 2132 226 152 2109 1697
Fragments arranged by size:
2132 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4870 160
Size: 872 3838 1606
Fragments arranged by size:
3838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 3641 1870
Size: 73 948 750 4545
Fragments arranged by size:
4545 948 750 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3434 3467 3434
Size: 33 6283
BamHI G'GATC_C
Cuts at: 2134 2913 3072 3646 4013 2134
Size: 779 159 574 367 4437
Fragments arranged by size:
4437 779 574 367 159
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3637 5092 618
Size: 325 217 627 1850 1455 1842
Fragments arranged by size:
1850 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2359 2396 2890 3635 1391
Size: 968 37 494 745 4072
Fragments arranged by size:
4072 968 745 494 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3197 3485 928
Size: 971 1298 288 3759
Fragments arranged by size:
3759 1298 971 288
BbvCI CC'TCA_GC
Cuts at: 2037 2037
Size: 6316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4507 4541 1903
Size: 34 2570 34 3678
Fragments arranged by size:
3678 2570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4208 5418 4208
Size: 1210 5106
BclI T'GATC_A
Cuts at: 1548 3103 1548
Size: 1555 4761
BfrBI ATG'CAT
Cuts at: 2089 3422 2089
Size: 1333 4983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4927 136
Size: 107 122 71 631 605 3255 1525
Fragments arranged by size:
3255 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6311 6311
Size: 6316
BlpI GC'TnA_GC
Cuts at: 3733 3733
Size: 6316
Bme1580I G_kGCm'C
Cuts at: 1790 2359 3216 4377 5533 1790
Size: 569 857 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 857 569
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6316
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 4047 5005
Size: 416 53 83 399 24 982 1648 958
Cuts at: 5005 442
Size: 1753
Fragments arranged by size:
1753 1648 982 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2430 2571 2939 3253 3449 4976 1239
Size: 1191 141 368 314 196 1527 2579
Fragments arranged by size:
2579 1527 1191 368 314 196 141
Bpu10I CC'TnA_GC
Cuts at: 2037 2641 3456 2037
Size: 604 815 4897
Fragments arranged by size:
4897 815 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3614 4441 5306 5604 3614
Size: 827 865 298 4326
Fragments arranged by size:
4326 865 827 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3425 4979 882
Size: 617 1926 1554 2219
Fragments arranged by size:
2219 1926 1554 617
BsaAI yAC'GTr
Cuts at: 493 1204 3211 4107 493
Size: 711 2007 896 2702
Fragments arranged by size:
2702 2007 896 711
BsaBI GATnn'nnATC
Cuts at: 6310 6310
Size: 6316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4123 4505 5140 275
Size: 53 83 186 936 2590 382 635 1451
Fragments arranged by size:
2590 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3504 4749 5147 5421 5568 5979 3504
Size: 1245 398 274 147 411 3841
Fragments arranged by size:
3841 1245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 3071 3101 3401
Size: 30 165 30 843 30 795 30 300
Cuts at: 3401 3431 1178
Size: 30 4063
Fragments arranged by size:
4063 843 795 300 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2292 2849 2909 3131 1186
Size: 1065 41 557 60 222 4371
Fragments arranged by size:
4371 1065 557 222 60 41
BseYI C'CCAG_C
Cuts at: 2879 5519 6015 2879
Size: 2640 496 3180
Fragments arranged by size:
3180 2640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 2728 1163
Size: 573 819 173 4751
Fragments arranged by size:
4751 819 573 173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 4527 5555 1540
Size: 275 186 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3216 4377 4462 5533 3216
Size: 1161 85 1071 3999
Fragments arranged by size:
3999 1161 1071 85
BsmI GAATG_Cn'
Cuts at: 3827 3920 3827
Size: 93 6223
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 2396 2890 3216 3635 4377 4462
Size: 399 569 37 494 326 419 742 85
Cuts at: 4462 5533 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 569 494 419 399 326
85 37
BspEI T'CCGG_A
Cuts at: 5979 5979
Size: 6316
BspHI T'CATG_A
Cuts at: 4205 5935 4205
Size: 1730 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3671 844
Size: 2827 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2018 4203 1394
Size: 429 195 2185 3507
Fragments arranged by size:
3507 2185 429 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 3144 4811 4985 65
Size: 1183 1896 1667 174 1396
Fragments arranged by size:
1896 1667 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 4960 5147 1700
Size: 110 255 2895 187 2869
Fragments arranged by size:
2895 2869 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 4376 5388 4376
Size: 1012 5304
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2930 794
Size: 2136 4180
BstBI TT'CG_AA
Cuts at: 5120 5120
Size: 6316
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6316
BstYI r'GATC_y
Cuts at: 1972 2134 2913 3072 3646 4013 4398 4415 5867
Size: 162 779 159 574 367 385 17 1452
Cuts at: 5867 5953 5965 6311 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 779 574 385 367 346 162
159 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3338 3338
Size: 6316
BtgI C'CryG_G
Cuts at: 513 1065 2421 513
Size: 552 1356 4408
Fragments arranged by size:
4408 1356 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5577
Fragments arranged by size:
5577 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 3832 4626 4646 2063
Size: 1769 794 20 3733
Fragments arranged by size:
3733 1769 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6281
DraI TTT'AAA
Cuts at: 3620 3620
Size: 6316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5323 809
Size: 507 4007 1802
Fragments arranged by size:
4007 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2802 3612 3763
Size: 54 1425 50 275 18 972 810 151
Cuts at: 3763 4652 6281 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 972 889 810 275 151 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 2939 3062 4246 1353
Size: 1457 129 123 1184 3423
Fragments arranged by size:
3423 1457 1184 129 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2184 4903 5277 5423 387
Size: 1064 138 595 2719 374 146 1280
Fragments arranged by size:
2719 1280 1064 595 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2430 2553 2571 2939 3253 3449 4379
Size: 393 798 123 18 368 314 196 930
Cuts at: 4379 4976 5763 6031 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 930 798 787 597 393 368 314
268 196 123 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2279
Size: 6316
EcoO109I rG'GnC_Cy
Cuts at: 2355 2356 2427 2566 2966 3373 3400 2355
Size: 1 71 139 400 407 27 5271
Fragments arranged by size:
5271 407 400 139 71 27 1
EcoRI G'AATT_C
Cuts at: 3625 4003 3625
Size: 378 5938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 6316
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 2761 5463 1678
Size: 1083 2702 2531
Fragments arranged by size:
2702 2531 1083
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2624 2656 3171
Size: 32 135 32 674 32 346 32 515
Cuts at: 3171 3203 3275 3307 4957 4989 5031 5063 1373
Size: 32 72 32 1650 32 42 32 2626
Fragments arranged by size:
2626 1650 674 515 346 135 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3660 3906 669
Size: 988 281 1722 246 3079
Fragments arranged by size:
3079 1722 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6316
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KpnI G_GTAC'C
Cuts at: 3641 3641
Size: 6316
MfeI C'AATT_G
Cuts at: 3915 3915
Size: 6316
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3405 5430 5614 1305
Size: 2100 2025 184 2007
Fragments arranged by size:
2100 2025 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 10
Size: 54 1425 343 972 3522
Fragments arranged by size:
3522 1425 972 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3311 3587 4274 4633 4792 518
Size: 648 1644 501 276 687 359 159 2042
Fragments arranged by size:
2042 1644 687 648 501 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2144 2232 2667 2764 2772 2845 3032
Size: 435 103 88 435 97 8 73 187
Cuts at: 3032 3732 4409 5557 5802 1606
Size: 700 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 435 435 245 187
103 97 88 73 8
NcoI C'CATG_G
Cuts at: 513 1065 2421 513
Size: 552 1356 4408
Fragments arranged by size:
4408 1356 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6316
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NsiI A_TGCA'T
Cuts at: 2091 3424 2091
Size: 1333 4983
NspI r_CATG'y
Cuts at: 1132 1229 2048 3674 5219 1132
Size: 97 819 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 819 97
PasI CC'CwG_GG
Cuts at: 1348 2281 2510 2597 1348
Size: 933 229 87 5067
Fragments arranged by size:
5067 933 229 87
PciI A'CATG_T
Cuts at: 5215 5215
Size: 6316
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2342 3610 1108
Size: 902 332 1268 3814
Fragments arranged by size:
3814 1268 902 332
PfoI T'CCnGG_A
Cuts at: 2916 2916
Size: 6316
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PmlI CAC'GTG
Cuts at: 3211 3211
Size: 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 5898 5930 4385
Size: 32 1481 32 4771
Fragments arranged by size:
4771 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2427 2966 3400 2427
Size: 539 434 5343
Fragments arranged by size:
5343 539 434
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 3886 3886
Size: 6316
PspOMI G'GGCC_C
Cuts at: 2355 2355
Size: 6316
PstI C_TGCA'G
Cuts at: 830 3668 830
Size: 2838 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
PvuII CAG'CTG
Cuts at: 2667 3032 2667
Size: 365 5951
SalI G'TCGA_C
Cuts at: 1936 3658 1936
Size: 1722 4594
SanDI GG'GwC_CC
Cuts at: 2427 2427
Size: 6316
SbfI CC_TGCA'GG
Cuts at: 3668 3668
Size: 6316
ScaI AGT'ACT
Cuts at: 2780 4564 6276 2780
Size: 1784 1712 2820
Fragments arranged by size:
2820 1784 1712
SfcI C'TryA_G
Cuts at: 826 1045 3664 4799 5480 5671 826
Size: 219 2619 1135 681 191 1471
Fragments arranged by size:
2619 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 3643 1872
Size: 1771 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3518 3593 4420 5321 5583 820
Size: 197 926 1575 75 827 901 262 1553
Fragments arranged by size:
1575 1553 926 901 827 262 197 75
SnaBI TAC'GTA
Cuts at: 493 4107 493
Size: 3614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 2048 3674 1132
Size: 916 1626 3774
Fragments arranged by size:
3774 1626 916
SspI AAT'ATT
Cuts at: 5 52 4240 5
Size: 47 4188 2081
Fragments arranged by size:
4188 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2341 2421 3369 3755 513
Size: 552 1276 80 948 386 3074
Fragments arranged by size:
3074 1276 948 552 386 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3249 4343 4360 4513 4698 5143 325
Size: 1255 1669 1094 17 153 185 445 1498
Fragments arranged by size:
1669 1498 1255 1094 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 4562 6274
Size: 274 80 33 51 1443 801 1784 1712
Cuts at: 6274 96
Size: 138
Fragments arranged by size:
1784 1712 1443 801 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3071 3103 6276 6308 3071
Size: 32 3173 32 3079
Fragments arranged by size:
3173 3079 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6316
XbaI T'CTAG_A
Cuts at: 3526 3652 3526
Size: 126 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6316
XmaI C'CCGG_G
Cuts at: 1870 3641 1870
Size: 1771 4545
XmnI GAAnn'nnTTC
Cuts at: 4445 4445
Size: 6316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4124 276
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI