Restriction map for
pFN21AB4051
(Circular) (Six-base) MAPSORT of: pFN21AB4051.seq Check: 8477 from: 1 to: 5728
pFN21AB4051 5728 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2426 2706 3791 5175 5443 1632
Size: 794 280 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 794 280 268
AflII C'TTAA_G
Cuts at: 820 1017 2498 820
Size: 197 1481 4050
Fragments arranged by size:
4050 1481 197
AflIII A'CryG_T
Cuts at: 1203 4627 5604 1203
Size: 3424 977 1327
Fragments arranged by size:
3424 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 2603 3471 5043 2603
Size: 868 1572 3288
Fragments arranged by size:
3288 1572 868
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 2118 3037 3263 3415 5524 1493
Size: 625 919 226 152 2109 1697
Fragments arranged by size:
2109 1697 919 625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 3053 1870
Size: 73 1110 4545
Fragments arranged by size:
4545 1110 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4504 618
Size: 325 217 627 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3047 1391
Size: 1656 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2530 3620 4830 2530
Size: 1090 1210 3428
Fragments arranged by size:
3428 1210 1090
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BfrBI ATG'CAT
Cuts at: 2223 2223
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2494 5723 2494
Size: 3229 2499
BlpI GC'TnA_GC
Cuts at: 2447 3145 2447
Size: 698 5030
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2458 3789 4945 1790
Size: 383 285 1331 1156 2573
Fragments arranged by size:
2573 1331 1156 383 285
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4417 442
Size: 416 53 83 399 24 2042 958 1753
Fragments arranged by size:
2042 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4388 1239
Size: 3149 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 2741 5722 2741
Size: 2981 2747
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4161 4559 4833 4980 5391 4161
Size: 398 274 147 411 4498
Fragments arranged by size:
4498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5503
Fragments arranged by size:
5503 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 1186
Size: 1205 4523
BseYI C'CCAG_C
Cuts at: 3018 4931 5427 3018
Size: 1913 496 3319
Fragments arranged by size:
3319 1913 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 1163
Size: 573 346 4809
Fragments arranged by size:
4809 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 2225 3239 3332 2225
Size: 1014 93 4621
Fragments arranged by size:
4621 1014 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2458 3047 3789 3874 4945 1391
Size: 399 383 285 589 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 589 399 383 285 85
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3083 844
Size: 2239 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2757 4223 4397 65
Size: 1183 1509 1466 174 1396
Fragments arranged by size:
1509 1466 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 2548
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2494 3058 3425 3810 3827 5279 5365 5377
Size: 522 564 367 385 17 1452 86 12
Cuts at: 5377 5723 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 564 522 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2952 2952
Size: 5728
BtgI C'CryG_G
Cuts at: 513 1065 2004 2260 513
Size: 552 939 256 3981
Fragments arranged by size:
3981 939 552 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 2115 2458 3244 4038 4058 2115
Size: 343 786 794 20 3785
Fragments arranged by size:
3785 794 786 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3007 3032 3007
Size: 25 5703
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693
Size: 54 1425 50 275 18 1345 889 1629
Cuts at: 5693 8
Size: 43
Fragments arranged by size:
1629 1425 1345 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3658 1353
Size: 2305 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 2706 3791 4388 5175 5443 1239
Size: 393 794 280 1085 597 787 268 1524
Fragments arranged by size:
1524 1085 794 787 597 393 280 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 2231
Size: 5728
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 5728
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 2340 4875 1678
Size: 662 2535 2531
Fragments arranged by size:
2535 2531 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 4369 4401 4443
Size: 32 135 32 607 32 2158 32 42
Cuts at: 4443 4475 1373
Size: 32 2626
Fragments arranged by size:
2626 2158 607 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 4842 5026 1305
Size: 1209 2328 184 2007
Fragments arranged by size:
2328 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2199 2885 3686 4045 4204 518
Size: 648 1033 686 801 359 159 2042
Fragments arranged by size:
2042 1033 801 686 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2446 3018 3144 3821 4969 5214 1606
Size: 840 572 126 677 1148 245 2120
Fragments arranged by size:
2120 1148 840 677 572 245 126
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NsiI A_TGCA'T
Cuts at: 2225 2225
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 2661 3086 4631 1132
Size: 97 1432 425 1545 2229
Fragments arranged by size:
2229 1545 1432 425 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PfoI T'CCnGG_A
Cuts at: 2402 2402
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2544 2576 2967 2999 3797 3829 5310 5342 2544
Size: 32 391 32 798 32 1481 32 2930
Fragments arranged by size:
2930 1481 798 391 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2578 2610 2578
Size: 32 5696
PstI C_TGCA'G
Cuts at: 830 3080 830
Size: 2250 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2446 3018 2446
Size: 572 5156
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SexAI A'CCwGG_T
Cuts at: 2528 2528
Size: 5728
SfcI C'TryA_G
Cuts at: 826 1045 3076 4211 4892 5083 826
Size: 219 2031 1135 681 191 1471
Fragments arranged by size:
2031 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 3832 4733 4995 820
Size: 197 926 555 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 555 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2004 3167 513
Size: 552 939 1163 3074
Fragments arranged by size:
3074 1163 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3755 3772 3925 4110 4555 325
Size: 1255 2175 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2992 3974 5686
Size: 274 80 33 51 1443 1015 982 1712
Cuts at: 5686 96
Size: 138
Fragments arranged by size:
1712 1443 1015 982 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI