Restriction map for
pFN21AB4048
(Circular) (Six-base) MAPSORT of: pFN21AB4048.seq Check: 6568 from: 1 to: 6493
pFN21AB4048 6493 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 3610 3814 3610
Size: 204 6289
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2741 3078 3189 3503 4556 5940 6208 1632
Size: 1109 337 111 314 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1109 1053 337 314 268 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 2172 5392 6369 1203
Size: 969 3220 977 1327
Fragments arranged by size:
3220 1327 977 969
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6493
AlwNI CAG_nnn'CTG
Cuts at: 4236 5808 4236
Size: 1572 4921
ApaLI G'TGCA_C
Cuts at: 2388 4550 5706 2388
Size: 2162 1156 3175
Fragments arranged by size:
3175 2162 1156
ApoI r'AATT_y
Cuts at: 1493 3273 3438 3641 3737 3802 4028 4180 6289
Size: 1780 165 203 96 65 226 152 2109
Cuts at: 6289 1493
Size: 1697
Fragments arranged by size:
2109 1780 1697 226 203 165 152 96
65
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 3818 1870
Size: 73 1875 4545
Fragments arranged by size:
4545 1875 73
BamHI G'GATC_C
Cuts at: 3823 4190 3823
Size: 367 6126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3610 3814 5269 618
Size: 325 217 627 1823 204 1455 1842
Fragments arranged by size:
1842 1823 1455 627 325 217 204
BanII G_rGCy'C
Cuts at: 1391 3319 3380 3812 1391
Size: 1928 61 432 4072
Fragments arranged by size:
4072 1928 432 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2747 928
Size: 971 848 4674
Fragments arranged by size:
4674 971 848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4684 4718 1903
Size: 34 50 34 2663 34 3678
Fragments arranged by size:
3678 2663 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 3727 1548
Size: 2179 4314
BfrBI ATG'CAT
Cuts at: 2629 2629
Size: 6493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5104 136
Size: 107 122 71 631 605 3432 1525
Fragments arranged by size:
3432 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6488 6488
Size: 6493
BlpI GC'TnA_GC
Cuts at: 3910 3910
Size: 6493
Bme1580I G_kGCm'C
Cuts at: 1790 2392 4554 5710 1790
Size: 602 2162 1156 2573
Fragments arranged by size:
2573 2162 1156 602
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4224 5182 442
Size: 416 53 83 399 24 2807 958 1753
Fragments arranged by size:
2807 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 3681 5153 1239
Size: 1180 1262 1472 2579
Fragments arranged by size:
2579 1472 1262 1180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4618 5483 5781 4618
Size: 865 298 5330
Fragments arranged by size:
5330 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2223 3012 3360 5156 882
Size: 617 724 789 348 1796 2219
Fragments arranged by size:
2219 1796 789 724 617 348
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 6487 6487
Size: 6493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2500 4300 4682 5317
Size: 53 83 186 936 967 1800 382 635
Cuts at: 5317 275
Size: 1451
Fragments arranged by size:
1800 1451 967 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4926 5324 5598 5745 6156 4926
Size: 398 274 147 411 5263
Fragments arranged by size:
5263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6268
Fragments arranged by size:
6268 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3030 3297 1186
Size: 1844 267 4382
Fragments arranged by size:
4382 1844 267
BseYI C'CCAG_C
Cuts at: 5696 6192 5696
Size: 496 5997
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2458 1163
Size: 573 722 5198
Fragments arranged by size:
5198 722 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 3943 4704 5732 1540
Size: 275 186 45 1897 761 1028 2301
Fragments arranged by size:
2301 1897 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 2392 2461 3330 3380 4554 4639 5710 2392
Size: 69 869 50 1174 85 1071 3175
Fragments arranged by size:
3175 1174 1071 869 85 69 50
BsmI GAATG_Cn'
Cuts at: 2581 2629 2959 4004 4097 2581
Size: 48 330 1045 93 4977
Fragments arranged by size:
4977 1045 330 93 48
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2461 3319 3330 3380 3812 4554
Size: 399 602 69 858 11 50 432 742
Cuts at: 4554 4639 5710 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 602 432 399 85
69 50 11
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 2112 2674 3112 4382 6112 2112
Size: 562 438 1270 1730 2493
Fragments arranged by size:
2493 1730 1270 562 438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3848 844
Size: 3004 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4380 1394
Size: 429 2557 3507
Fragments arranged by size:
3507 2557 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 2718 2853 3261 4988 5162 65
Size: 1183 916 554 135 408 1727 174 1396
Fragments arranged by size:
1727 1396 1183 916 554 408 174 135
BsrFI r'CCGG_y
Cuts at: 1700 1810 5137 5324 1700
Size: 110 3327 187 2869
Fragments arranged by size:
3327 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2237 96
Size: 2141 4352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2523 2587 794
Size: 1729 64 4700
Fragments arranged by size:
4700 1729 64
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6493
BstYI r'GATC_y
Cuts at: 1972 3823 4190 4575 4592 6044 6130 6142 6488
Size: 1851 367 385 17 1452 86 12 346
Cuts at: 6488 1972
Size: 1977
Fragments arranged by size:
1977 1851 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3507 3507
Size: 6493
BtgI C'CryG_G
Cuts at: 513 1065 2101 2610 513
Size: 552 1036 509 4396
Fragments arranged by size:
4396 1036 552 509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5754
Fragments arranged by size:
5754 683 56
BtsI GCAGTG_nn'
Cuts at: 4009 4803 4823 4009
Size: 794 20 5679
Fragments arranged by size:
5679 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3622 3797 3622
Size: 175 6318
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2422 3218 3940
Size: 54 1425 50 275 18 592 796 722
Cuts at: 3940 4829 6458 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 796 722 592 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3940 1537
Size: 275 2128 4090
Fragments arranged by size:
4090 2128 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 2782 3419 3479 4423 1353
Size: 851 578 637 60 944 3423
Fragments arranged by size:
3423 944 851 637 578 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 5454 5600 387
Size: 1064 138 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3378 3378
Size: 6493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 2741 3078 3189 3503 3681 4556
Size: 393 787 322 337 111 314 178 875
Cuts at: 4556 5153 5940 6208 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 875 787 787 597 393 337 322
314 268 178 111
EcoNI CCTnn'n_nnAGG
Cuts at: 2355 3534 2355
Size: 1179 5314
EcoO109I rG'GnC_Cy
Cuts at: 2149 3502 2149
Size: 1353 5140
EcoRI G'AATT_C
Cuts at: 3802 4180 3802
Size: 378 6115
EcoRV GAT'ATC
Cuts at: 2110 2110
Size: 6493
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 5640 1678
Size: 3962 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2370 2606 2638 2671
Size: 32 135 32 766 32 236 32 33
Cuts at: 2671 2703 2915 2947 5134 5166 5208 5240 1373
Size: 32 212 32 2187 32 42 32 2626
Fragments arranged by size:
2626 2187 766 236 212 135 42 33
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3307 3837 4083 669
Size: 988 281 1369 530 246 3079
Fragments arranged by size:
3079 1369 988 530 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 2736 748
Size: 1657 331 4505
Fragments arranged by size:
4505 1657 331
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 3614 3818 3614
Size: 204 6289
MfeI C'AATT_G
Cuts at: 2565 4092 2565
Size: 1527 4966
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 5607 5791 1305
Size: 930 3372 184 2007
Fragments arranged by size:
3372 2007 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2424 10
Size: 54 1425 343 592 4079
Fragments arranged by size:
4079 1425 592 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4451 4810 4969 518
Size: 648 3285 359 159 2042
Fragments arranged by size:
3285 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2103 2397 3909 4586 5734 5979 1606
Size: 455 42 294 1512 677 1148 245 2120
Fragments arranged by size:
2120 1512 1148 677 455 294 245 42
NcoI C'CATG_G
Cuts at: 513 1065 2610 513
Size: 552 1545 4396
Fragments arranged by size:
4396 1545 552
NdeI CA'TA_TG
Cuts at: 387 3033 387
Size: 2646 3847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NsiI A_TGCA'T
Cuts at: 2631 2631
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 2176 2918 3068 3202 3851 5396 1132
Size: 97 947 742 150 134 649 1545 2229
Fragments arranged by size:
2229 1545 947 742 649 150 134 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6493
PciI A'CATG_T
Cuts at: 2172 5392 2172
Size: 3220 3273
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2211 1108
Size: 1103 5390
PfoI T'CCnGG_A
Cuts at: 2356 2356
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3502 3502
Size: 6493
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2799 2831 2799
Size: 32 6461
PstI C_TGCA'G
Cuts at: 830 3085 3781 3845 830
Size: 2255 696 64 3478
Fragments arranged by size:
3478 2255 696 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
SacI G_AGCT'C
Cuts at: 3380 3380
Size: 6493
SacII CC_GC'GG
Cuts at: 2104 2104
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 6493
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 2808 4741 6453 2808
Size: 1933 1712 2848
Fragments arranged by size:
2848 1933 1712
SexAI A'CCwGG_T
Cuts at: 2076 2076
Size: 6493
SfcI C'TryA_G
Cuts at: 826 1045 3081 3777 3841 4976 5657 5848 826
Size: 219 2036 696 64 1135 681 191 1471
Fragments arranged by size:
2036 1471 1135 696 681 219 191 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4597 5498 5760 820
Size: 197 926 2654 901 262 1553
Fragments arranged by size:
2654 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3851 1132
Size: 2719 3774
SspI AAT'ATT
Cuts at: 5 52 3631 4417 5
Size: 47 3579 786 2081
Fragments arranged by size:
3579 2081 786 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6493
StyI C'CwwG_G
Cuts at: 513 1065 2503 2610 3932 513
Size: 552 1438 107 1322 3074
Fragments arranged by size:
3074 1438 1322 552 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2496 4520 4537 4690 4875 5320 325
Size: 1255 916 2024 17 153 185 445 1498
Fragments arranged by size:
2024 1498 1255 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2237 2806 4739
Size: 274 80 33 51 1443 260 569 1933
Cuts at: 4739 6451 96
Size: 1712 138
Fragments arranged by size:
1933 1712 1443 569 274 260 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6453 6485 6453
Size: 32 6461
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 2809
Size: 6493
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PvuII RsrII SanDI SfiI
SfoI SrfI SwaI