Restriction map for
pFN21AB4046
(Circular) (Six-base) MAPSORT of: pFN21AB4046.seq Check: 4247 from: 1 to: 6040
pFN21AB4046 6040 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3100 3100
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3090 3167 3383 1937
Size: 1153 77 216 4594
Fragments arranged by size:
4594 1153 216 77
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2711 4103 5487 5755 1632
Size: 398 681 1392 1384 268 1917
Fragments arranged by size:
1917 1392 1384 681 398 268
AfeI AGC'GCT
Cuts at: 2128 2128
Size: 6040
AflII C'TTAA_G
Cuts at: 820 1017 3224 820
Size: 197 2207 3636
Fragments arranged by size:
3636 2207 197
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 3226 4769 3226
Size: 1543 4497
AleI CACnn'nnGTG
Cuts at: 1166 3310 1166
Size: 2144 3896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2534 1184
Size: 1350 4690
AlwNI CAG_nnn'CTG
Cuts at: 2105 3783 5355 2105
Size: 1678 1572 2790
Fragments arranged by size:
2790 1678 1572
ApaLI G'TGCA_C
Cuts at: 3020 4097 5253 3020
Size: 1077 1156 3807
Fragments arranged by size:
3807 1156 1077
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 3365 1870
Size: 73 214 1208 4545
Fragments arranged by size:
4545 1208 214 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 2518 3298 3361 4816
Size: 325 217 627 473 258 780 63 1455
Cuts at: 4816 618
Size: 1842
Fragments arranged by size:
1842 1455 780 627 473 325 258 217
63
BanII G_rGCy'C
Cuts at: 1391 2406 2829 2847 3359 1391
Size: 1015 423 18 512 4072
Fragments arranged by size:
4072 1015 512 423 18
BbeI G_GCGC'C
Cuts at: 3302 3302
Size: 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2584 928
Size: 971 685 4384
Fragments arranged by size:
4384 971 685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4231 4265 1903
Size: 34 50 34 2210 34 3678
Fragments arranged by size:
3678 2210 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2851 4651 136
Size: 107 122 71 631 605 1179 1800 1525
Fragments arranged by size:
1800 1525 1179 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2925 6035 2925
Size: 3110 2930
BlpI GC'TnA_GC
Cuts at: 2400 2835 3457 2400
Size: 435 622 4983
Fragments arranged by size:
4983 622 435
Bme1580I G_kGCm'C
Cuts at: 1790 2265 2439 3024 4101 5257 1790
Size: 475 174 585 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 585 475 174
BmgBI CAC'GTC
Cuts at: 1318 3312 1318
Size: 1994 4046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3095
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2660 2861 4700 1239
Size: 980 441 201 1839 2579
Fragments arranged by size:
2579 1839 980 441 201
Bpu10I CC'TnA_GC
Cuts at: 2504 2769 2504
Size: 265 5775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2914 2975 4165 5030 5328 2914
Size: 61 1190 865 298 3626
Fragments arranged by size:
3626 1190 865 298 61
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 2324 6034 2324
Size: 3710 2330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2757 3299 3847
Size: 53 83 186 936 651 573 542 548
Cuts at: 3847 4229 4864 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 651 635 573 548 542 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3135 4473 4871 5145 5292 5703 3135
Size: 1338 398 274 147 411 3472
Fragments arranged by size:
3472 1338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2834 2864 1178
Size: 30 165 30 1431 30 4354
Fragments arranged by size:
4354 1431 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2456 1186
Size: 883 387 4770
Fragments arranged by size:
4770 883 387
BseYI C'CCAG_C
Cuts at: 2098 2283 2574 2581 3030 3102 3145 5243 5739
Size: 185 291 7 449 72 43 2098 496
Cuts at: 5739 2098
Size: 2399
Fragments arranged by size:
2399 2098 496 449 291 185 72 43
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3182 1163
Size: 573 1446 4021
Fragments arranged by size:
4021 1446 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2619 2888 3490 4251 5279 1540
Size: 275 186 618 269 602 761 1028 2301
Fragments arranged by size:
2301 1028 761 618 602 275 269 186
BsiHKAI G_wGCw'C
Cuts at: 2406 2829 3024 3100 4101 4186 5257 2406
Size: 423 195 76 1001 85 1071 3189
Fragments arranged by size:
3189 1071 1001 423 195 85 76
BsmI GAATG_Cn'
Cuts at: 2071 3551 3644 2071
Size: 1480 93 4467
Fragments arranged by size:
4467 1480 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2438 2722 2438
Size: 284 5756
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2406 2439 2829 2847 3024 3100
Size: 399 475 141 33 390 18 177 76
Cuts at: 3100 3359 4101 4186 5257 1391
Size: 259 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 399 390 259 177
141 85 76 33 18
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 3100 3395 844
Size: 2213 43 295 3489
Fragments arranged by size:
3489 2213 295 43
BsrBI CCG'CTC
Cuts at: 1394 1823 2203 3927 1394
Size: 429 380 1724 3507
Fragments arranged by size:
3507 1724 429 380
BsrDI GCAATG_nn'
Cuts at: 65 1248 2511 4535 4709 65
Size: 1183 1263 2024 174 1396
Fragments arranged by size:
2024 1396 1263 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2311 4684 4871 1700
Size: 110 501 2373 187 2869
Fragments arranged by size:
2869 2373 501 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 2561 1925
Size: 636 5404
BssSI C'ACGA_G
Cuts at: 2438 4100 5112 2438
Size: 1662 1012 3366
Fragments arranged by size:
3366 1662 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3019 794
Size: 2225 3815
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 3205 3312 3205
Size: 107 5933
BstYI r'GATC_y
Cuts at: 1972 2925 3370 3737 4122 4139 5591 5677 5689
Size: 953 445 367 385 17 1452 86 12
Cuts at: 5689 6035 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 953 445 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3168 3168
Size: 6040
BtgI C'CryG_G
Cuts at: 513 1065 2268 2613 2619 2965 513
Size: 552 1203 345 6 346 3588
Fragments arranged by size:
3588 1203 552 346 345 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 2443 487
Size: 683 56 1094 123 4084
Fragments arranged by size:
4084 1094 683 123 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2616 2820 2852
Size: 54 1425 50 275 18 786 204 32
Cuts at: 2852 2885 2947 3487 4376 6005 8
Size: 33 62 540 889 1629 43
Fragments arranged by size:
1629 1425 889 786 540 275 204 62
54 50 43 33 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2616 2885 3487 1537
Size: 275 804 269 602 4090
Fragments arranged by size:
4090 804 602 275 269
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2411 3970 1353
Size: 734 324 1559 3423
Fragments arranged by size:
3423 1559 734 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2404 2827 2404
Size: 423 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2219 2660 2711 2861 4103 4700
Size: 393 398 189 441 51 150 1242 597
Cuts at: 4700 5487 5755 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1242 787 597 441 398 393 268
189 150 51
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 2046 2130 2365 2582 3302 5187 1678
Size: 368 84 235 217 720 1885 2531
Fragments arranged by size:
2531 1885 720 368 235 217 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 3212 3244 4681
Size: 32 135 32 653 32 955 32 1437
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 955 653 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KasI G'GCGC_C
Cuts at: 3298 3298
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2854 10
Size: 54 1425 343 1022 3196
Fragments arranged by size:
3196 1425 1022 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 3310 3998 4357 4516 518
Size: 648 1350 794 688 359 159 2042
Fragments arranged by size:
2042 1350 794 688 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2270 2574 2615 2686 2896 3034 3055
Size: 645 19 304 41 71 210 138 21
Cuts at: 3055 3106 3456 4133 5281 5526 1606
Size: 51 350 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 645 350 304 245 210
138 71 51 41 21 19
NaeI GCC'GGC
Cuts at: 2313 2313
Size: 6040
NarI GG'CG_CC
Cuts at: 3299 3299
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5488
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NgoMIV G'CCGG_C
Cuts at: 2311 2311
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2105 1108
Size: 997 5043
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2026 2223 3054 3111 3392 830
Size: 1196 197 831 57 281 3478
Fragments arranged by size:
3478 1196 831 281 197 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 3034 3106 3034
Size: 72 5968
SacI G_AGCT'C
Cuts at: 2406 2829 2406
Size: 423 5617
SacII CC_GC'GG
Cuts at: 2271 2616 2271
Size: 345 5695
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SapI GCTCTTCn'nnn_
Cuts at: 2411 2411
Size: 6040
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 2666 4288 6000 2666
Size: 1622 1712 2706
Fragments arranged by size:
2706 1712 1622
SfcI C'TryA_G
Cuts at: 826 1045 2022 2219 3050 3107 3388 4523 5204
Size: 219 977 197 831 57 281 1135 681
Cuts at: 5204 5395 826
Size: 191 1471
Fragments arranged by size:
1471 1135 977 831 681 281 219 197
191 57
SfoI GGC'GCC
Cuts at: 3300 3300
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 2311 1700
Size: 611 5429
SmaI CCC'GGG
Cuts at: 1872 2159 3367 1872
Size: 287 1208 4545
Fragments arranged by size:
4545 1208 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2929 2990 3224 4144 5045 5307
Size: 197 926 986 61 234 920 901 262
Cuts at: 5307 820
Size: 1553
Fragments arranged by size:
1553 986 926 920 901 262 234 197
61
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 2767 2861 3062 1517
Size: 1250 94 201 4495
Fragments arranged by size:
4495 1250 201 94
StyI C'CwwG_G
Cuts at: 513 1065 3198 3479 513
Size: 552 2133 281 3074
Fragments arranged by size:
3074 2133 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3044 3084 3197 4067 4084 4237 4422
Size: 1255 1464 40 113 870 17 153 185
Cuts at: 4422 4867 325
Size: 445 1498
Fragments arranged by size:
1498 1464 1255 870 445 185 153 113
40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 4286 5998
Size: 274 80 33 51 1443 687 1622 1712
Cuts at: 5998 96
Size: 138
Fragments arranged by size:
1712 1622 1443 687 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2834 2866 6000 6032 2834
Size: 32 3134 32 2842
Fragments arranged by size:
3134 2842 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2862 2862
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 2157 3365 1870
Size: 287 1208 4545
Fragments arranged by size:
4545 1208 287
XmnI GAAnn'nnTTC
Cuts at: 2166 4169 2166
Size: 2003 4037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SanDI SexAI SfiI SrfI SwaI