Restriction map for
pFN21AB4044
(Circular) (Six-base) MAPSORT of: pFN21AB4044.seq Check: 736 from: 1 to: 6253
pFN21AB4044 6253 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2598 2598
Size: 6253
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4063 278
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3596 1937
Size: 1659 4594
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 4380 4753 1563
Size: 2817 373 3063
Fragments arranged by size:
3063 2817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4316 5700 5968 1632
Size: 2684 1384 268 1917
Fragments arranged by size:
2684 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6056
AflIII A'CryG_T
Cuts at: 1203 2287 2433 3377 5152 6129 1203
Size: 1084 146 944 1775 977 1327
Fragments arranged by size:
1775 1327 1084 977 944 146
AgeI A'CCGG_T
Cuts at: 5084 5084
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 4982 4982
Size: 6253
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6253
AlwNI CAG_nnn'CTG
Cuts at: 2590 3521 3996 5568 2590
Size: 931 475 1572 3275
Fragments arranged by size:
3275 1572 931 475
ApaLI G'TGCA_C
Cuts at: 3445 4310 5466 3445
Size: 865 1156 4232
Fragments arranged by size:
4232 1156 865
ApoI r'AATT_y
Cuts at: 1493 2313 2861 2996 3562 3788 3940 6049 1493
Size: 820 548 135 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 820 566 548 226 152 135
AseI AT'TA_AT
Cuts at: 160 1032 2097 4807 160
Size: 872 1065 2710 1606
Fragments arranged by size:
2710 1606 1065 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 3578 1870
Size: 73 1635 4545
Fragments arranged by size:
4545 1635 73
AvrII C'CTAG_G
Cuts at: 3470 3470
Size: 6253
BamHI G'GATC_C
Cuts at: 2545 3583 3950 2545
Size: 1038 367 4848
Fragments arranged by size:
4848 1038 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3574 5029 618
Size: 325 217 627 1787 1455 1842
Fragments arranged by size:
1842 1787 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2752 3277 3509 3572 1391
Size: 1361 525 232 63 4072
Fragments arranged by size:
4072 1361 525 232 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4444 4478 1903
Size: 34 50 34 2423 34 3678
Fragments arranged by size:
3678 2423 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 5355 4145
Size: 1210 5043
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4864 136
Size: 107 122 71 631 605 3192 1525
Fragments arranged by size:
3192 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2820 6248 2820
Size: 3428 2825
BlpI GC'TnA_GC
Cuts at: 2977 3670 2977
Size: 693 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2799 3449 4314 5470 1790
Size: 1009 650 865 1156 2573
Fragments arranged by size:
2573 1156 1009 865 650
BmgBI CAC'GTC
Cuts at: 1318 2434 1318
Size: 1116 5137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3304 3984 4942
Size: 416 53 83 399 24 1887 680 958
Cuts at: 4942 442
Size: 1753
Fragments arranged by size:
1887 1753 958 680 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2561 4913 1239
Size: 824 498 2352 2579
Fragments arranged by size:
2579 2352 824 498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3128 4378 5243 5541 3128
Size: 1250 865 298 3840
Fragments arranged by size:
3840 1250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4916 882
Size: 617 3417 2219
Fragments arranged by size:
3417 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4044 493
Size: 711 2840 2702
Fragments arranged by size:
2840 2702 711
BsaBI GATnn'nnATC
Cuts at: 6247 6247
Size: 6253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4060 4442 5077 275
Size: 53 83 186 936 2527 382 635 1451
Fragments arranged by size:
2527 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4686 5084 5358 5505 5916 4686
Size: 398 274 147 411 5023
Fragments arranged by size:
5023 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6028
Fragments arranged by size:
6028 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2760 1186
Size: 1574 4679
BseYI C'CCAG_C
Cuts at: 2102 3279 5456 5952 2102
Size: 1177 2177 496 2403
Fragments arranged by size:
2403 2177 1177 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 1163
Size: 573 840 4840
Fragments arranged by size:
4840 840 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4464 5492 1540
Size: 275 186 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2366 2517 2752 3449 4314 4399 5470 2366
Size: 151 235 697 865 85 1071 3149
Fragments arranged by size:
3149 1071 865 697 235 151 85
BsmI GAATG_Cn'
Cuts at: 3443 3764 3857 3443
Size: 321 93 5839
Fragments arranged by size:
5839 321 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2366 2517 2752 2799 3277 3449 3509
Size: 399 576 151 235 47 478 172 60
Cuts at: 3509 3572 4314 4399 5470 1391
Size: 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 576 478 399 235 172
151 85 63 60 47
BspEI T'CCGG_A
Cuts at: 5916 5916
Size: 6253
BspHI T'CATG_A
Cuts at: 4142 5872 4142
Size: 1730 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 3341 3608 844
Size: 1754 743 267 3489
Fragments arranged by size:
3489 1754 743 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3091 4140 1394
Size: 429 1268 1049 3507
Fragments arranged by size:
3507 1268 1049 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4748 4922 65
Size: 1183 3500 174 1396
Fragments arranged by size:
3500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4897 5084 1700
Size: 110 3087 187 2869
Fragments arranged by size:
3087 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6253
BssSI C'ACGA_G
Cuts at: 4313 5325 4313
Size: 1012 5241
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3490 794
Size: 2696 3557
BstBI TT'CG_AA
Cuts at: 2611 5057 2611
Size: 2446 3807
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6253
BstYI r'GATC_y
Cuts at: 1972 2545 2556 2820 3583 3950 4335 4352 5804
Size: 573 11 264 763 367 385 17 1452
Cuts at: 5804 5890 5902 6248 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 763 573 385 367 346 264
86 17 12 11
BtgI C'CryG_G
Cuts at: 513 1065 2799 513
Size: 552 1734 3967
Fragments arranged by size:
3967 1734 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5514
Fragments arranged by size:
5514 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3769 4563 4583 2503
Size: 1266 794 20 4173
Fragments arranged by size:
4173 1266 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 2079 2340 2406 3430 3557 2079
Size: 261 66 1024 127 4775
Fragments arranged by size:
4775 1024 261 127 66
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5260 809
Size: 507 3944 1802
Fragments arranged by size:
3944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2488 3458 3700
Size: 54 1425 50 275 18 658 970 242
Cuts at: 3700 4589 6218 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 970 889 658 275 242 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3700 1537
Size: 275 1888 4090
Fragments arranged by size:
4090 1888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3044 3165 4183 1353
Size: 1691 121 1018 3423
Fragments arranged by size:
3423 1691 1018 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2750 2750
Size: 6253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2561 4316 4913 5700 5968 1239
Size: 393 431 498 1755 597 787 268 1524
Fragments arranged by size:
1755 1524 787 597 498 431 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3410 3518 3410
Size: 108 6145
EcoRI G'AATT_C
Cuts at: 3562 3940 3562
Size: 378 5875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2432 2702 2432
Size: 270 5983
FspI TGC'GCA
Cuts at: 4759 4759
Size: 6253
HaeII r_GCGC'y
Cuts at: 1678 5400 1678
Size: 3722 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3236 3268 4894 4926 4968
Size: 32 135 32 1664 32 1626 32 42
Cuts at: 4968 5000 1373
Size: 32 2626
Fragments arranged by size:
2626 1664 1626 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 3597 3843 669
Size: 988 281 1084 575 246 3079
Fragments arranged by size:
3079 1084 988 575 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6253
HpaI GTT'AAC
Cuts at: 3843 3843
Size: 6253
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2228 5367 5551 1305
Size: 923 3139 184 2007
Fragments arranged by size:
3139 2007 923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2490 10
Size: 54 1425 343 658 3773
Fragments arranged by size:
3773 1425 658 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2307 2427 4211 4570 4729 518
Size: 648 1120 21 120 1784 359 159 2042
Fragments arranged by size:
2042 1784 1120 648 359 159 120 21
MspA1I CmG'CkG
Cuts at: 1606 3084 3279 3669 4346 5494 5739 1606
Size: 1478 195 390 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 390 245 195
NcoI C'CATG_G
Cuts at: 513 1065 2799 513
Size: 552 1734 3967
Fragments arranged by size:
3967 1734 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 2291 2306 3381 3491 3611 5156 1132
Size: 97 1062 15 1075 110 120 1545 2229
Fragments arranged by size:
2229 1545 1075 1062 120 110 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6253
PciI A'CATG_T
Cuts at: 2287 3377 5152 2287
Size: 1090 1775 3388
Fragments arranged by size:
3388 1775 1090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 1108
Size: 1431 4822
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6253
PsiI TTA'TAA
Cuts at: 3823 3823
Size: 6253
PstI C_TGCA'G
Cuts at: 830 2389 2623 3605 830
Size: 1559 234 982 3478
Fragments arranged by size:
3478 1559 982 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
SacI G_AGCT'C
Cuts at: 2752 2752
Size: 6253
SalI G'TCGA_C
Cuts at: 1936 3595 1936
Size: 1659 4594
SbfI CC_TGCA'GG
Cuts at: 3605 3605
Size: 6253
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SfcI C'TryA_G
Cuts at: 826 1045 2385 2619 3601 4736 5417 5608 826
Size: 219 1340 234 982 1135 681 191 1471
Fragments arranged by size:
1471 1340 1135 982 681 234 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6253
SmaI CCC'GGG
Cuts at: 1872 3580 1872
Size: 1708 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3143 4357 5258 5520 820
Size: 197 926 1200 1214 901 262 1553
Fragments arranged by size:
1553 1214 1200 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4044 493
Size: 3551 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6253
SphI G_CATG'C
Cuts at: 1132 3611 1132
Size: 2479 3774
SspI AAT'ATT
Cuts at: 5 52 4177 5
Size: 47 4125 2081
Fragments arranged by size:
4125 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 1517
Size: 654 5599
StyI C'CwwG_G
Cuts at: 513 1065 2029 2799 2968 3114 3470 3692 513
Size: 552 964 770 169 146 356 222 3074
Fragments arranged by size:
3074 964 770 552 356 222 169 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4280 4297 4450 4635 5080 325
Size: 1255 2700 17 153 185 445 1498
Fragments arranged by size:
2700 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2135 3063 4499
Size: 274 80 33 51 1443 158 928 1436
Cuts at: 4499 6211 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1436 928 274 158 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2629 2661 2699 2731 3428 3460 6213 6245 2629
Size: 32 38 32 697 32 2753 32 2637
Fragments arranged by size:
2753 2637 697 38 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6253
XbaI T'CTAG_A
Cuts at: 3031 3589 3031
Size: 558 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 3146 2671
Size: 475 5778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 3578 1870
Size: 1708 4545
XmnI GAAnn'nnTTC
Cuts at: 3174 4382 3174
Size: 1208 5045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4061 276
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI