Restriction map for
pFN21AB4038
(Circular) (Six-base) MAPSORT of: pFN21AB4038.seq Check: 4829 from: 1 to: 5794
pFN21AB4038 5794 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2634 3137 1937
Size: 697 503 4594
Fragments arranged by size:
4594 697 503
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3857 5241 5509 1632
Size: 2225 1384 268 1917
Fragments arranged by size:
2225 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2035 1184
Size: 851 4943
AlwNI CAG_nnn'CTG
Cuts at: 2887 3537 5109 2887
Size: 650 1572 3572
Fragments arranged by size:
3572 1572 650
ApaI G_GGCC'C
Cuts at: 2250 2250
Size: 5794
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2209 3103 3329 3481 5590 1493
Size: 716 894 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 716 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2897 3115 4570 618
Size: 325 217 627 1110 218 1455 1842
Fragments arranged by size:
1842 1455 1110 627 325 218 217
BanII G_rGCy'C
Cuts at: 1391 2161 2250 2307 3113 1391
Size: 770 89 57 806 4072
Fragments arranged by size:
4072 806 770 89 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2910 3076 928
Size: 971 1011 166 3646
Fragments arranged by size:
3646 1011 971 166
BbvCI CC'TCA_GC
Cuts at: 2664 2664
Size: 5794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4405 136
Size: 107 122 71 631 605 337 2396 1525
Fragments arranged by size:
2396 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2784 2955 5789 2784
Size: 171 2834 2789
Fragments arranged by size:
2834 2789 171
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 2250 3855 5011 1790
Size: 460 1605 1156 2573
Fragments arranged by size:
2573 1605 1156 460
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2671 3525 4483
Size: 416 53 83 399 24 1254 854 958
Cuts at: 4483 442
Size: 1753
Fragments arranged by size:
1753 1254 958 854 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2321
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2488 2660 2765 2864 3011 4454 1239
Size: 1249 172 105 99 147 1443 2579
Fragments arranged by size:
2579 1443 1249 172 147 105 99
Bpu10I CC'TnA_GC
Cuts at: 2472 2664 2472
Size: 192 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 3919 4784 5082 2350
Size: 1569 865 298 3062
Fragments arranged by size:
3062 1569 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4457 882
Size: 617 1167 1791 2219
Fragments arranged by size:
2219 1791 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3601 3983 4618 275
Size: 53 83 186 936 2068 382 635 1451
Fragments arranged by size:
2068 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3087 4227 4625 4899 5046 5457 3087
Size: 1140 398 274 147 411 3424
Fragments arranged by size:
3424 1140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2517 2547 2933 2963 1178
Size: 30 165 30 1114 30 386 30 4009
Fragments arranged by size:
4009 1114 386 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2315 2954 1186
Size: 880 249 639 4026
Fragments arranged by size:
4026 880 639 249
BseYI C'CCAG_C
Cuts at: 2493 2569 4997 5493 2493
Size: 76 2428 496 2794
Fragments arranged by size:
2794 2428 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2532 1163
Size: 573 796 4425
Fragments arranged by size:
4425 796 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2397 3855 3940 5011 2307
Size: 90 1458 85 1071 3090
Fragments arranged by size:
3090 1458 1071 90 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2250 2307 2397 3113 3855 3940
Size: 399 371 89 57 90 716 742 85
Cuts at: 3940 5011 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 716 399 371 90 89
85 57
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 2307 3683 5413 2307
Size: 1376 1730 2688
Fragments arranged by size:
2688 1730 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2024 2198 2668 3149 844
Size: 1180 174 470 481 3489
Fragments arranged by size:
3489 1180 481 470 174
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4289 4463 65
Size: 1183 3041 174 1396
Fragments arranged by size:
3041 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3054 4438 4625 1700
Size: 110 1244 1384 187 2869
Fragments arranged by size:
2869 1384 1244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 2442 3854 4866 2442
Size: 1412 1012 3370
Fragments arranged by size:
3370 1412 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 3007 1184
Size: 1823 3971
BstYI r'GATC_y
Cuts at: 1972 2784 2955 3124 3491 3876 3893 5345 5431
Size: 812 171 169 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 812 385 367 346 171 169
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2049 2049
Size: 5794
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 4104 4124 3310
Size: 794 20 4980
Fragments arranged by size:
4980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2134 2169 529
Size: 35 1570 35 4154
Fragments arranged by size:
4154 1570 35 35
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2702 4801 809
Size: 507 1386 2099 1802
Fragments arranged by size:
2099 1802 1386 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2407 2602
Size: 54 1425 50 275 18 345 232 195
Cuts at: 2602 2649 2998 3241 4130 5759 8
Size: 47 349 243 889 1629 43
Fragments arranged by size:
1629 1425 889 349 345 275 243 232
195 54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2545 3724 1353
Size: 1026 166 1179 3423
Fragments arranged by size:
3423 1179 1026 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2305 2305
Size: 5794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2488 2660 2765 2864 3011 3857 4454
Size: 393 856 172 105 99 147 846 597
Cuts at: 4454 5241 5509 1239
Size: 787 268 1524
Fragments arranged by size:
1524 856 846 787 597 393 268 172
147 105 99
EcoO109I rG'GnC_Cy
Cuts at: 2056 2247 2718 2056
Size: 191 471 5132
Fragments arranged by size:
5132 471 191
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 4941 1678
Size: 3263 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2355 2482 2514 2663
Size: 32 135 32 751 32 127 32 149
Cuts at: 2663 2695 2776 2808 4435 4467 4509 4541 1373
Size: 32 81 32 1627 32 42 32 2626
Fragments arranged by size:
2626 1627 751 149 135 127 81 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2806 3138 3384 669
Size: 988 281 868 332 246 3079
Fragments arranged by size:
3079 988 868 332 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 4908 5092 1305
Size: 813 2790 184 2007
Fragments arranged by size:
2790 2007 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2409 2604 2651 3000 10
Size: 54 1425 343 577 195 47 349 2804
Fragments arranged by size:
2804 1425 577 349 343 195 54 47
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 3752 4111 4270 518
Size: 648 1146 1440 359 159 2042
Fragments arranged by size:
2042 1440 1146 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2776 3210 3887 5035 5280 1606
Size: 1170 434 677 1148 245 2120
Fragments arranged by size:
2120 1170 1148 677 434 245
NaeI GCC'GGC
Cuts at: 3056 3056
Size: 5794
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5242
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NgoMIV G'CCGG_C
Cuts at: 3054 3054
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3083 3152 4697 1132
Size: 97 1854 69 1545 2229
Fragments arranged by size:
2229 1854 1545 97 69
PasI CC'CwG_GG
Cuts at: 1348 2068 2099 1348
Size: 720 31 5043
Fragments arranged by size:
5043 720 31
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2887 1108
Size: 1779 4015
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2056
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2246 2246
Size: 5794
PstI C_TGCA'G
Cuts at: 830 3146 830
Size: 2316 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 2776 2776
Size: 5794
RsrII CG'GwC_CG
Cuts at: 3090 3090
Size: 5794
SacI G_AGCT'C
Cuts at: 2307 2307
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SapI GCTCTTCn'nnn_
Cuts at: 2379 2379
Size: 5794
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SexAI A'CCwGG_T
Cuts at: 2886 2886
Size: 5794
SfcI C'TryA_G
Cuts at: 826 1045 2283 3142 4277 4958 5149 826
Size: 219 1238 859 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 859 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2329 3898 4799 5061 820
Size: 197 926 386 1569 901 262 1553
Fragments arranged by size:
1569 1553 926 901 386 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 2087 2483 2760 1517
Size: 570 396 277 4551
Fragments arranged by size:
4551 570 396 277
StyI C'CwwG_G
Cuts at: 513 1065 2404 3233 513
Size: 552 1339 829 3074
Fragments arranged by size:
3074 1339 829 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2542 2986 3821 3838 3991 4176 4621
Size: 1255 962 444 835 17 153 185 445
Cuts at: 4621 325
Size: 1498
Fragments arranged by size:
1498 1255 962 835 445 444 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2517 2548 2549 2580 2933 2965 5754 5786 2517
Size: 31 1 31 353 32 2789 32 2525
Fragments arranged by size:
2789 2525 353 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 2677 1316
Size: 1361 4433
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2240 2240
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI SacII SanDI SfiI SfoI SrfI
SwaI