Restriction map for
pFN21AB4019
(Circular) (Six-base) MAPSORT of: pFN21AB4019.seq Check: 1402 from: 1 to: 5995
pFN21AB4019 5995 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 3294 4122 4495 1563
Size: 1731 828 373 3063
Fragments arranged by size:
3063 1731 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4058 5442 5710 1632
Size: 2426 1384 268 1917
Fragments arranged by size:
2426 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 3021 4894 5871 1203
Size: 1818 1873 977 1327
Fragments arranged by size:
1873 1818 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 2537 1166
Size: 1371 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2052 1184
Size: 868 5127
AlwNI CAG_nnn'CTG
Cuts at: 3738 5310 3738
Size: 1572 4423
ApaI G_GGCC'C
Cuts at: 3088 3088
Size: 5995
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 2719 2894 3304 3530 3682 5791 1493
Size: 1226 175 410 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 410 226 175 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3320 1870
Size: 73 1377 4545
Fragments arranged by size:
4545 1377 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4771 618
Size: 325 217 627 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2290 3088 3314 1391
Size: 899 798 226 4072
Fragments arranged by size:
4072 899 798 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2288 3112 3146 3887 5097 2288
Size: 824 34 741 1210 3186
Fragments arranged by size:
3186 1210 824 741 34
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BfrBI ATG'CAT
Cuts at: 2029 2029
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 3088 4056 5212 1790
Size: 1298 968 1156 2573
Fragments arranged by size:
2573 1298 1156 968
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2781 3726 4684
Size: 416 53 83 399 24 1364 945 958
Cuts at: 4684 442
Size: 1753
Fragments arranged by size:
1753 1364 958 945 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2480
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2433 2519 3054 4655 1239
Size: 1194 86 535 1601 2579
Fragments arranged by size:
2579 1601 1194 535 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 4120 4985 5283 2633
Size: 1487 865 298 3345
Fragments arranged by size:
3345 1487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4658 882
Size: 617 3159 2219
Fragments arranged by size:
3159 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2539 2661 3786 493
Size: 711 1335 122 1125 2702
Fragments arranged by size:
2702 1335 1125 711 122
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3802 4184 4819 275
Size: 53 83 186 936 2269 382 635 1451
Fragments arranged by size:
2269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2871 4428 4826 5100 5247 5658 2871
Size: 1557 398 274 147 411 3208
Fragments arranged by size:
3208 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3087 3117 1178
Size: 30 165 30 1684 30 4056
Fragments arranged by size:
4056 1684 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2298 2309 2819 1186
Size: 1112 11 510 4362
Fragments arranged by size:
4362 1112 510 11
BseYI C'CCAG_C
Cuts at: 2039 2073 2313 5198 5694 2039
Size: 34 240 2885 496 2340
Fragments arranged by size:
2885 2340 496 240 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3074 1163
Size: 573 1338 4084
Fragments arranged by size:
4084 1338 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2660 4056 4141 5212 2660
Size: 1396 85 1071 3443
Fragments arranged by size:
3443 1396 1071 85
BsmI GAATG_Cn'
Cuts at: 2029 2443 3506 3599 2029
Size: 414 1063 93 4425
Fragments arranged by size:
4425 1063 414 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2707 2707
Size: 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2290 2660 3088 3314 4056 4141 5212
Size: 399 500 370 428 226 742 85 1071
Cuts at: 5212 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 500 428 399 370 226
85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3045 3884 5614 3045
Size: 839 1730 3426
Fragments arranged by size:
3426 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 3350 844
Size: 1567 939 3489
Fragments arranged by size:
3489 1567 939
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 3882 1394
Size: 429 776 1283 3507
Fragments arranged by size:
3507 1283 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4490 4664 65
Size: 1183 3242 174 1396
Fragments arranged by size:
3242 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4639 4826 1700
Size: 110 2829 187 2869
Fragments arranged by size:
2869 2829 187 110
BsrGI T'GTAC_A
Cuts at: 96 3183 96
Size: 3087 2908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990
Size: 1353 367 385 17 1452 86 12 346
Cuts at: 5990 1972
Size: 1977
Fragments arranged by size:
1977 1452 1353 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2959 513
Size: 552 939 955 3549
Fragments arranged by size:
3549 955 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 2937 3277 3511 4305 4325 2937
Size: 340 234 794 20 4607
Fragments arranged by size:
4607 794 340 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2803 2838 529
Size: 35 2239 35 3686
Fragments arranged by size:
3686 2239 35 35
DraI TTT'AAA
Cuts at: 2208 3299 2208
Size: 1091 4904
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 3442 4331
Size: 54 1425 50 275 18 222 1390 889
Cuts at: 4331 5960 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1390 889 275 222 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2705 3925 1353
Size: 1352 1220 3423
Fragments arranged by size:
3423 1352 1220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2388 2722 4582 4956 5102 387
Size: 1064 138 799 334 1860 374 146 1280
Fragments arranged by size:
1860 1280 1064 799 374 334 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2519 3054 4058 4655 5442 5710
Size: 393 801 86 535 1004 597 787 268
Cuts at: 5710 1239
Size: 1524
Fragments arranged by size:
1524 1004 801 787 597 535 393 268
86
EcoNI CCTnn'n_nnAGG
Cuts at: 3258 3258
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 2360 3084 3263 2360
Size: 724 179 5092
Fragments arranged by size:
5092 724 179
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 5995
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 5142 1678
Size: 3464 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3172 3204 4636 4668 4710
Size: 32 135 32 1600 32 1432 32 42
Cuts at: 4710 4742 1373
Size: 32 2626
Fragments arranged by size:
2626 1600 1432 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3073 3315 5109 5293 1305
Size: 1768 242 1794 184 2007
Fragments arranged by size:
2007 1794 1768 242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2034 2537 2639 3050 3953 4312 4471
Size: 648 868 503 102 411 903 359 159
Cuts at: 4471 518
Size: 2042
Fragments arranged by size:
2042 903 868 648 503 411 359 159
102
MspA1I CmG'CkG
Cuts at: 1606 2317 2390 2399 2933 3411 4088 5236 5481
Size: 711 73 9 534 478 677 1148 245
Cuts at: 5481 1606
Size: 2120
Fragments arranged by size:
2120 1148 711 677 534 478 245 73
9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2959 513
Size: 552 939 955 3549
Fragments arranged by size:
3549 955 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NsiI A_TGCA'T
Cuts at: 2031 2031
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2204 2338 3025 3353 4898 1132
Size: 97 975 134 687 328 1545 2229
Fragments arranged by size:
2229 1545 975 687 328 134 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 3021 4894 3021
Size: 1873 4122
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 1108
Size: 1068 4927
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PmlI CAC'GTG
Cuts at: 2539 2661 2539
Size: 122 5873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 3084 3084
Size: 5995
PstI C_TGCA'G
Cuts at: 830 3347 830
Size: 2517 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2317 2933 2317
Size: 616 5379
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 3255 3343 4478 5159 5350 826
Size: 219 2210 88 1135 681 191 1471
Fragments arranged by size:
2210 1471 1135 681 219 191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2648 4099 5000 5262 820
Size: 197 926 705 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 705 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 2756 2914 3919 5
Size: 47 2704 158 1005 2081
Fragments arranged by size:
2704 2081 1005 158 47
StuI AGG'CCT
Cuts at: 1517 2516 1517
Size: 999 4996
StyI C'CwwG_G
Cuts at: 513 1065 2004 2959 3079 3434 513
Size: 552 939 955 120 355 3074
Fragments arranged by size:
3074 955 939 552 355 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2549 4022 4039 4192 4377 4822
Size: 1255 537 432 1473 17 153 185 445
Cuts at: 4822 325
Size: 1498
Fragments arranged by size:
1498 1473 1255 537 445 432 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3183 4241 5953
Size: 274 80 33 51 1443 1206 1058 1712
Cuts at: 5953 96
Size: 138
Fragments arranged by size:
1712 1443 1206 1058 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3087 3119 5955 5987 3087
Size: 32 2836 32 3095
Fragments arranged by size:
3095 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI