Restriction map for
pFN21AB4017
(Circular) (Six-base) MAPSORT of: pFN21AB4017.seq Check: 6275 from: 1 to: 6097
pFN21AB4017 6097 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3215 3215
Size: 6097
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2404 3254 4160 5544 5812 1632
Size: 772 850 906 1384 268 1917
Fragments arranged by size:
1917 1384 906 850 772 268
AfeI AGC'GCT
Cuts at: 2585 2585
Size: 6097
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 3090 4996 5973 1203
Size: 1887 1906 977 1327
Fragments arranged by size:
1906 1887 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 3083 1166
Size: 1917 4180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2234 2549 1184
Size: 1050 315 4732
Fragments arranged by size:
4732 1050 315
AlwNI CAG_nnn'CTG
Cuts at: 2194 2320 3232 3840 5412 2194
Size: 126 912 608 1572 2879
Fragments arranged by size:
2879 1572 912 608 126
ApaI G_GGCC'C
Cuts at: 2090 2090
Size: 6097
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 3406 3632 3784 5893 1493
Size: 1913 226 152 2109 1697
Fragments arranged by size:
2109 1913 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 3132 3422 1870
Size: 73 147 1042 290 4545
Fragments arranged by size:
4545 1042 290 147 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2635 3418 4873 618
Size: 325 217 627 319 529 783 1455 1842
Fragments arranged by size:
1842 1455 783 627 529 325 319 217
BanII G_rGCy'C
Cuts at: 1391 2090 2097 2270 2891 3279 3416 1391
Size: 699 7 173 621 388 137 4072
Fragments arranged by size:
4072 699 621 388 173 137 7
BbeI G_GCGC'C
Cuts at: 2110 2110
Size: 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 3149 928
Size: 971 115 1135 3876
Fragments arranged by size:
3876 1135 971 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2801 2835 4288 4322 1903
Size: 34 864 34 1453 34 3678
Fragments arranged by size:
3678 1453 864 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 3312 1548
Size: 1764 4333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2949 3062
Size: 107 122 71 631 605 527 750 113
Cuts at: 3062 3125 4708 136
Size: 63 1583 1525
Fragments arranged by size:
1583 1525 750 631 605 527 122 113
107 71 63
BglII A'GATC_T
Cuts at: 2325 2568 6092 2325
Size: 243 3524 2330
Fragments arranged by size:
3524 2330 243
BlpI GC'TnA_GC
Cuts at: 2694 3514 2694
Size: 820 5277
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2640 4158 5314 1790
Size: 300 550 1518 1156 2573
Fragments arranged by size:
2573 1518 1156 550 300
BmgBI CAC'GTC
Cuts at: 1318 3085 3213 1318
Size: 1767 128 4202
Fragments arranged by size:
4202 1767 128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2108 3828 4786
Size: 416 53 83 399 24 691 1720 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1720 958 691 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2353 2923 4757 1239
Size: 1114 570 1834 2579
Fragments arranged by size:
2579 1834 1114 570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 4760 882
Size: 617 543 2718 2219
Fragments arranged by size:
2718 2219 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2107 3146 3904 4286
Size: 53 83 186 936 574 1039 758 382
Cuts at: 4286 4921 275
Size: 635 1451
Fragments arranged by size:
1451 1039 936 758 635 574 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3048 4530 4928 5202 5349 5760 3048
Size: 1482 398 274 147 411 3385
Fragments arranged by size:
3385 1482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2363 2393 2990
Size: 30 165 30 897 30 33 30 597
Cuts at: 2990 3020 1178
Size: 30 4255
Fragments arranged by size:
4255 897 597 165 33 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2530 3014 3017 1186
Size: 1192 152 484 3 4266
Fragments arranged by size:
4266 1192 484 152 3
BseYI C'CCAG_C
Cuts at: 2639 2720 3332 5300 5796 2639
Size: 81 612 1968 496 2940
Fragments arranged by size:
2940 1968 612 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2992 3193 1163
Size: 573 1256 201 4067
Fragments arranged by size:
4067 1256 573 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2209 2632 3547 4308 5336 1540
Size: 275 186 208 423 915 761 1028 2301
Fragments arranged by size:
2301 1028 915 761 423 275 208 186
BsiHKAI G_wGCw'C
Cuts at: 2097 3279 4158 4243 5314 2097
Size: 1182 879 85 1071 2880
Fragments arranged by size:
2880 1182 1071 879 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2097 2270 2640 2891 3279 3416
Size: 399 300 7 173 370 251 388 137
Cuts at: 3416 4158 4243 5314 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 370 300 251
173 137 85 7
BspEI T'CCGG_A
Cuts at: 3048 5760 3048
Size: 2712 3385
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2772 3215 3452 844
Size: 1928 443 237 3489
Fragments arranged by size:
3489 1928 443 237
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 3984 1394
Size: 429 235 1926 3507
Fragments arranged by size:
3507 1926 429 235
BsrDI GCAATG_nn'
Cuts at: 65 1248 2482 4592 4766 65
Size: 1183 1234 2110 174 1396
Fragments arranged by size:
2110 1396 1234 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2392 2703 4741 4928 1700
Size: 110 582 311 2038 187 2869
Fragments arranged by size:
2869 2038 582 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 2484 794
Size: 1526 164 4407
Fragments arranged by size:
4407 1526 164
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 2395 2848 1184
Size: 1211 453 4433
Fragments arranged by size:
4433 1211 453
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2758 3068 2011
Size: 747 310 5040
Fragments arranged by size:
5040 747 310
BstYI r'GATC_y
Cuts at: 1972 2325 2568 3427 3794 4179 4196 5648 5734
Size: 353 243 859 367 385 17 1452 86
Cuts at: 5734 5746 6092 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 859 385 367 353 346 243
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3020 3020
Size: 6097
BtgI C'CryG_G
Cuts at: 513 1065 2098 513
Size: 552 1033 4512
Fragments arranged by size:
4512 1033 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2195 2321 3613 4407 4427 2195
Size: 126 1292 794 20 3865
Fragments arranged by size:
3865 1292 794 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2462 3367 5104 809
Size: 507 1146 905 1737 1802
Fragments arranged by size:
1802 1737 1146 905 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2223 2808
Size: 54 1425 50 275 18 234 159 585
Cuts at: 2808 3544 4433 6062 8
Size: 736 889 1629 43
Fragments arranged by size:
1629 1425 889 736 585 275 234 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2669 4027 1353
Size: 683 633 1358 3423
Fragments arranged by size:
3423 1358 683 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2095 3277 2095
Size: 1182 4915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2353 2404 2923 3254 4160 4757 5544
Size: 393 721 51 519 331 906 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 906 787 721 597 519 393 331
268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3324 3324
Size: 6097
EcoO109I rG'GnC_Cy
Cuts at: 2086 2087 2611 3104 3127 3329 2086
Size: 1 524 493 23 202 4854
Fragments arranged by size:
4854 524 493 202 23 1
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2110 2472 2587 2701 5244 1678
Size: 432 362 115 114 2543 2531
Fragments arranged by size:
2543 2531 432 362 115 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373
Size: 32 135 32 3166 32 42 32 2626
Fragments arranged by size:
3166 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2106 2106
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 2824 3696 2824
Size: 872 5225
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 5211 5395 1305
Size: 876 3030 184 2007
Fragments arranged by size:
3030 2007 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 2225 10
Size: 54 1425 343 234 159 3882
Fragments arranged by size:
3882 1425 343 234 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2957 3083 4055 4414 4573 518
Size: 648 1791 126 972 359 159 2042
Fragments arranged by size:
2042 1791 972 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2272 2286 2724 2856 2976 2988 3513 4190
Size: 666 14 438 132 120 12 525 677
Cuts at: 4190 5338 5583 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 525 438 245 132
120 14 12
NaeI GCC'GGC
Cuts at: 2705 2705
Size: 6097
NarI GG'CG_CC
Cuts at: 2107 2107
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5545
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NgoMIV G'CCGG_C
Cuts at: 2703 2703
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2662 3265 3455 5000 1132
Size: 97 1433 603 190 1545 2229
Fragments arranged by size:
2229 1545 1433 603 190 97
PasI CC'CwG_GG
Cuts at: 1348 3296 1348
Size: 1948 4149
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 4717
PfoI T'CCnGG_A
Cuts at: 3219 3219
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3194 1613
Size: 1581 4516
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2086 2086
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2085 2165 2316 2325 3449 830
Size: 1255 80 151 9 1124 3478
Fragments arranged by size:
3478 1255 1124 151 80 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2724 2856 2976 2724
Size: 132 120 5845
Fragments arranged by size:
5845 132 120
SacI G_AGCT'C
Cuts at: 2097 3279 2097
Size: 1182 4915
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SapI GCTCTTCn'nnn_
Cuts at: 2669 2669
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 2085 3449 2085
Size: 1364 4733
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2081 2161 2312 2321 3445 4580 5261
Size: 219 1036 80 151 9 1124 1135 681
Cuts at: 5261 5452 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1124 1036 681 219 191 151
80 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3125 3125
Size: 6097
SfoI GGC'GCC
Cuts at: 2108 2108
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 2662 3455 1132
Size: 1530 793 3774
Fragments arranged by size:
3774 1530 793
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 2504 3065 1517
Size: 987 561 4549
Fragments arranged by size:
4549 987 561
StyI C'CwwG_G
Cuts at: 513 1065 2247 2499 3393 3536 513
Size: 552 1182 252 894 143 3074
Fragments arranged by size:
3074 1182 894 552 252 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 2787 3137 4124 4141 4294 4479
Size: 1255 789 418 350 987 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1255 987 789 445 418 350 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 4343 6055
Size: 274 80 33 51 1443 669 1697 1712
Cuts at: 6055 96
Size: 138
Fragments arranged by size:
1712 1697 1443 669 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2300 2332 2990 3022 6057 6089 2300
Size: 32 658 32 3035 32 2308
Fragments arranged by size:
3035 2308 658 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 1316
Size: 807 5290
XbaI T'CTAG_A
Cuts at: 2041 3433 2041
Size: 1392 4705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2953 2953
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SrfI SwaI