Restriction map for
pFN21AB4014
(Circular) (Six-base) MAPSORT of: pFN21AB4014.seq Check: 5621 from: 1 to: 6112
pFN21AB4014 6112 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2758 2758
Size: 6112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 2932 3433 2932
Size: 501 5611
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3012 3149 4175 5559 5827 1632
Size: 1380 137 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1380 1026 268 137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 3243 1166
Size: 2077 4035
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2177 2345 2399 2711 2759 3855 5427 2177
Size: 168 54 312 48 1096 1572 2862
Fragments arranged by size:
2862 1572 1096 312 168 54 48
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 3254 3421 3647 3799 5908 1493
Size: 1761 167 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2157 2190 2157
Size: 33 6079
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 2932 2984 3318 3433
Size: 325 217 627 515 630 52 334 115
Cuts at: 3433 4888 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 630 627 515 334 325 217
115 52
BanII G_rGCy'C
Cuts at: 1391 2393 2978 3431 1391
Size: 1002 585 453 4072
Fragments arranged by size:
4072 1002 585 453
BbeI G_GCGC'C
Cuts at: 3322 3322
Size: 6112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2982 4723 136
Size: 107 122 71 631 605 1310 1741 1525
Fragments arranged by size:
1741 1525 1310 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 2900 3266 4173 5329 1790
Size: 1110 366 907 1156 2573
Fragments arranged by size:
2573 1156 1110 907 366
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2387 3843 4801
Size: 416 53 83 399 24 970 1456 958
Cuts at: 4801 442
Size: 1753
Fragments arranged by size:
1753 1456 970 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4772 1239
Size: 3533 2579
Bpu10I CC'TnA_GC
Cuts at: 2421 2421
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2268 4237 5102 5400 2268
Size: 1969 865 298 2980
Fragments arranged by size:
2980 1969 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2703 2746 4775 882
Size: 617 1204 43 2029 2219
Fragments arranged by size:
2219 2029 1204 617 43
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3919 4301 4936
Size: 53 83 186 936 1786 600 382 635
Cuts at: 4936 275
Size: 1451
Fragments arranged by size:
1786 1451 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2965 4545 4943 5217 5364 5775 2965
Size: 1580 398 274 147 411 3302
Fragments arranged by size:
3302 1580 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3385 3415 1178
Size: 30 165 30 1982 30 3875
Fragments arranged by size:
3875 1982 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2841 3036 3304 1186
Size: 1655 195 268 3994
Fragments arranged by size:
3994 1655 268 195
BseYI C'CCAG_C
Cuts at: 2233 2457 2843 2855 2937 5315 5811 2233
Size: 224 386 12 82 2378 496 2534
Fragments arranged by size:
2534 2378 496 386 224 82 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2634 2969 1163
Size: 573 898 335 4306
Fragments arranged by size:
4306 898 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4173 4258 5329 4173
Size: 85 1071 4956
Fragments arranged by size:
4956 1071 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
BsmBI CGTCTCn'nnnn_
Cuts at: 2675 2675
Size: 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2393 2900 2978 3266 3431 4173 4258
Size: 399 603 507 78 288 165 742 85
Cuts at: 4258 5329 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 603 507 399 288 165
85 78
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 3285 4001 5731 3285
Size: 716 1730 3666
Fragments arranged by size:
3666 1730 716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 2685 2758 3467 844
Size: 1642 199 73 709 3489
Fragments arranged by size:
3489 1642 709 199 73
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 3000 3378 4607 4781 65
Size: 1183 1110 642 378 1229 174 1396
Fragments arranged by size:
1396 1229 1183 1110 642 378 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2782 3276 4756 4943 1700
Size: 110 972 494 1480 187 2869
Fragments arranged by size:
2869 1480 972 494 187 110
BsrGI T'GTAC_A
Cuts at: 96 2320 96
Size: 2224 3888
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 3139 4172 5184 3139
Size: 1033 1012 4067
Fragments arranged by size:
4067 1033 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2648 2759 794
Size: 1854 111 4147
Fragments arranged by size:
4147 1854 111
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2597 3442 3809 4194 4211 5663 5749 5761
Size: 625 845 367 385 17 1452 86 12
Cuts at: 5761 6107 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 845 625 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4621
Fragments arranged by size:
4621 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 3628 4422 4442 3628
Size: 794 20 5298
Fragments arranged by size:
5298 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3115 3416 3115
Size: 301 5811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3559 4448 6077
Size: 54 1425 50 275 18 1729 889 1629
Cuts at: 6077 8
Size: 43
Fragments arranged by size:
1729 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 4042 1353
Size: 834 1855 3423
Fragments arranged by size:
3423 1855 834
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3012 3149 4175 4772 5559 5827 1239
Size: 393 1380 137 1026 597 787 268 1524
Fragments arranged by size:
1524 1380 1026 787 597 393 268 137
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2312 2919 3322 5259 1678
Size: 634 607 403 1937 2531
Fragments arranged by size:
2531 1937 634 607 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3058 3090 3203 3235 4753
Size: 32 135 32 1486 32 113 32 1518
Cuts at: 4753 4785 4827 4859 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1518 1486 135 113 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KasI G'GCGC_C
Cuts at: 3318 3318
Size: 6112
KpnI G_GTAC'C
Cuts at: 2936 3437 2936
Size: 501 5611
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2105 3416 5226 5410 1305
Size: 800 1311 1810 184 2007
Fragments arranged by size:
2007 1810 1311 800 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4290
Fragments arranged by size:
4290 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 2927 3243 4070 4429 4588 518
Size: 648 1378 383 316 827 359 159 2042
Fragments arranged by size:
2042 1378 827 648 383 359 316 159
MspA1I CmG'CkG
Cuts at: 1606 2450 2582 3171 3528 4205 5353 5598 1606
Size: 844 132 589 357 677 1148 245 2120
Fragments arranged by size:
2120 1148 844 677 589 357 245 132
NarI GG'CG_CC
Cuts at: 3319 3319
Size: 6112
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4621
Fragments arranged by size:
4621 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 3314 3470 5015 1132
Size: 97 2085 156 1545 2229
Fragments arranged by size:
2229 2085 1545 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3098 1108
Size: 1990 4122
PfoI T'CCnGG_A
Cuts at: 2057 2206 2057
Size: 149 5963
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2834 1613
Size: 1221 4891
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PstI C_TGCA'G
Cuts at: 830 2074 2123 2696 2755 2764 3464 830
Size: 1244 49 573 59 9 700 3478
Fragments arranged by size:
3478 1244 700 573 59 49 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2450 3171 2450
Size: 721 5391
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2070 2094 2119 2692 2751 2760 3082
Size: 219 1025 24 25 573 59 9 322
Cuts at: 3082 3172 3460 4595 5276 5467 826
Size: 90 288 1135 681 191 1471
Fragments arranged by size:
1471 1135 1025 681 573 322 288 219
191 90 59 25 24 9
SfoI GGC'GCC
Cuts at: 3320 3320
Size: 6112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2283 4216 5117 5379 820
Size: 197 926 340 1933 901 262 1553
Fragments arranged by size:
1933 1553 926 901 340 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3314 3470 1132
Size: 2182 156 3774
Fragments arranged by size:
3774 2182 156
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2004 3551 513
Size: 552 939 1547 3074
Fragments arranged by size:
3074 1547 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3105 4139 4156 4309 4494 4939 325
Size: 1255 1525 1034 17 153 185 445 1498
Fragments arranged by size:
1525 1498 1255 1034 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 4358 6070
Size: 274 80 33 51 1443 343 2038 1712
Cuts at: 6070 96
Size: 138
Fragments arranged by size:
2038 1712 1443 343 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3172 3204 6072 6104 3172
Size: 32 2868 32 3180
Fragments arranged by size:
3180 2868 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3095 3095
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI