Restriction map for
pFN21AB4011
(Circular) (Six-base) MAPSORT of: pFN21AB4011.seq Check: 1058 from: 1 to: 6358
pFN21AB4011 6358 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2145 2145
Size: 6358
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4168 278
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3701 1937
Size: 1764 4594
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2327 2432 2654 2748 2828 3215 4421
Size: 635 60 105 222 94 80 387 1206
Cuts at: 4421 5805 6073 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1206 635 387 268 222 105
94 80 60
AfeI AGC'GCT
Cuts at: 3110 3110
Size: 6358
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 2611 5257 6234 1203
Size: 1408 2646 977 1327
Fragments arranged by size:
2646 1408 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 3240 1166
Size: 2074 4284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 1184
Size: 1206 5152
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 6326
AlwNI CAG_nnn'CTG
Cuts at: 3094 3253 4101 5673 3094
Size: 159 848 1572 3779
Fragments arranged by size:
3779 1572 848 159
ApaI G_GGCC'C
Cuts at: 2040 2040
Size: 6358
ApaLI G'TGCA_C
Cuts at: 2158 3539 4415 5571 2158
Size: 1381 876 1156 2945
Fragments arranged by size:
2945 1381 1156 876
ApoI r'AATT_y
Cuts at: 1493 3667 3893 4045 6154 1493
Size: 2174 226 152 2109 1697
Fragments arranged by size:
2174 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 3683 1870
Size: 73 474 1266 4545
Fragments arranged by size:
4545 1266 474 73
BamHI G'GATC_C
Cuts at: 2133 2845 3688 4055 2133
Size: 712 843 367 4436
Fragments arranged by size:
4436 843 712 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2780 3234 3340 3679 5134
Size: 325 217 627 993 454 106 339 1455
Cuts at: 5134 618
Size: 1842
Fragments arranged by size:
1842 1455 993 627 454 339 325 217
106
BanII G_rGCy'C
Cuts at: 1391 2040 2127 2319 2528 3677 1391
Size: 649 87 192 209 1149 4072
Fragments arranged by size:
4072 1149 649 209 192 87
BbeI G_GCGC'C
Cuts at: 3344 3344
Size: 6358
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3001 3035 4549 4583 1903
Size: 34 50 34 980 34 1514 34 3678
Fragments arranged by size:
3678 1514 980 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3080 4969 136
Size: 107 122 71 631 605 1408 1889 1525
Fragments arranged by size:
1889 1525 1408 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6353 6353
Size: 6358
BlpI GC'TnA_GC
Cuts at: 2394 3775 2394
Size: 1381 4977
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2162 2554 2774 3303 3543 4419 5575
Size: 250 122 392 220 529 240 876 1156
Cuts at: 5575 1790
Size: 2573
Fragments arranged by size:
2573 1156 876 529 392 250 240 220
122
BmgBI CAC'GTC
Cuts at: 1318 2542 1318
Size: 1224 5134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2564 4089 5047
Size: 416 53 83 399 24 1147 1525 958
Cuts at: 5047 442
Size: 1753
Fragments arranged by size:
1753 1525 1147 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 3533 5018 1239
Size: 869 1425 1485 2579
Fragments arranged by size:
2579 1485 1425 869
Bpu10I CC'TnA_GC
Cuts at: 2059 3161 2059
Size: 1102 5256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4483 5348 5646 4483
Size: 865 298 5195
Fragments arranged by size:
5195 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2009 5021 882
Size: 617 510 3012 2219
Fragments arranged by size:
3012 2219 617 510
BsaAI yAC'GTr
Cuts at: 493 1204 2614 4149 493
Size: 711 1410 1535 2702
Fragments arranged by size:
2702 1535 1410 711
BsaBI GATnn'nnATC
Cuts at: 6352 6352
Size: 6358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2576 3341 4165 4547
Size: 53 83 186 936 1043 765 824 382
Cuts at: 4547 5182 275
Size: 635 1451
Fragments arranged by size:
1451 1043 936 824 765 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4791 5189 5463 5610 6021 4791
Size: 398 274 147 411 5128
Fragments arranged by size:
5128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2114 2144 1178
Size: 30 165 30 678 30 3 30 5392
Fragments arranged by size:
5392 678 165 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 3005 3215 1186
Size: 1705 114 210 4329
Fragments arranged by size:
4329 1705 210 114
BseYI C'CCAG_C
Cuts at: 2170 3253 3490 5561 6057 2170
Size: 1083 237 2071 496 2471
Fragments arranged by size:
2471 2071 1083 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3443 1163
Size: 573 1707 4078
Fragments arranged by size:
4078 1707 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3808 4569 5597 1540
Size: 275 186 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2319 2528 3282 3543 4419 4504 5575 2162
Size: 157 209 754 261 876 85 1071 2945
Fragments arranged by size:
2945 1071 876 754 261 209 157 85
BsmI GAATG_Cn'
Cuts at: 3869 3962 3869
Size: 93 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2127 2162 2319 2528 2554 2774
Size: 399 250 87 35 157 209 26 220
Cuts at: 2774 3282 3303 3543 3677 4419 4504 5575 1391
Size: 508 21 240 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 250 240 220
209 157 134 87 85 35 26 21
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 4247 5977 4247
Size: 1730 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 3713 844
Size: 1301 1568 3489
Fragments arranged by size:
3489 1568 1301
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 3646 4245 1394
Size: 429 235 1588 599 3507
Fragments arranged by size:
3507 1588 599 429 235
BsrDI GCAATG_nn'
Cuts at: 65 1248 2793 4853 5027 65
Size: 1183 1545 2060 174 1396
Fragments arranged by size:
2060 1545 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2175 5002 5189 1700
Size: 110 365 2827 187 2869
Fragments arranged by size:
2869 2827 365 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 3542 4418 5430 3542
Size: 876 1012 4470
Fragments arranged by size:
4470 1012 876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3094 794
Size: 2300 4058
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 3604 1184
Size: 2420 3938
BstXI CCAn_nnnn'nTGG
Cuts at: 2947 2947
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2133 2721 2845 3312 3471 3549 3688 4055
Size: 161 588 124 467 159 78 139 367
Cuts at: 4055 4440 4457 5909 5995 6007 6353 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 588 467 385 367 346 161
159 139 124 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2940 3345 513
Size: 552 1875 405 3526
Fragments arranged by size:
3526 1875 552 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5619
Fragments arranged by size:
5619 683 56
BtsI GCAGTG_nn'
Cuts at: 2205 2487 2738 3055 3874 4668 4688 2205
Size: 282 251 317 819 794 20 3875
Fragments arranged by size:
3875 819 794 317 282 251 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 3662 3662
Size: 6358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 3072 3633
Size: 54 1425 50 275 18 492 750 561
Cuts at: 3633 3805 4694 6323 8
Size: 172 889 1629 43
Fragments arranged by size:
1629 1425 889 750 561 492 275 172
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4288 1353
Size: 2935 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3464 4945 5319 5465 387
Size: 1064 138 1875 1481 374 146 1280
Fragments arranged by size:
1875 1481 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2526 2317
Size: 209 6149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2267 2327 2432 2654 2748 2828
Size: 393 476 159 60 105 222 94 80
Cuts at: 2828 3215 3533 4421 5018 5805 6073 1239
Size: 387 318 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 476 393 387 318
268 222 159 105 94 80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 2090
Size: 6358
EcoO109I rG'GnC_Cy
Cuts at: 2036 2097 3205 2036
Size: 61 1108 5189
Fragments arranged by size:
5189 1108 61
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
FseI GG_CCGG'CC
Cuts at: 2179 2179
Size: 6358
FspI TGC'GCA
Cuts at: 3087 4864 3087
Size: 1777 4581
HaeII r_GCGC'y
Cuts at: 1678 3112 3344 5505 1678
Size: 1434 232 2161 2531
Fragments arranged by size:
2531 2161 1434 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2426 2458 2476
Size: 32 135 32 509 32 313 32 18
Cuts at: 2476 2508 2817 2849 4999 5031 5073 5105 1373
Size: 32 309 32 2150 32 42 32 2626
Fragments arranged by size:
2626 2150 509 313 309 135 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3702 3948 669
Size: 988 281 1764 246 3079
Fragments arranged by size:
3079 1764 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KasI G'GCGC_C
Cuts at: 3340 3340
Size: 6358
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5472 5656 1305
Size: 4167 184 2007
Fragments arranged by size:
4167 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 10
Size: 54 1425 343 492 4044
Fragments arranged by size:
4044 1425 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2504 2507 2756 2840 3240 4316
Size: 648 1101 237 3 249 84 400 1076
Cuts at: 4316 4675 4834 518
Size: 359 159 2042
Fragments arranged by size:
2042 1101 1076 648 400 359 249 237
159 84 3
MspA1I CmG'CkG
Cuts at: 1606 2170 2714 3271 3583 3774 4451 5599 5844
Size: 564 544 557 312 191 677 1148 245
Cuts at: 5844 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 564 557 544 312 245
191
NaeI GCC'GGC
Cuts at: 2177 2177
Size: 6358
NarI GG'CG_CC
Cuts at: 3341 3341
Size: 6358
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5806
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NgoMIV G'CCGG_C
Cuts at: 2175 2175
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 2755 3016 3397 3716 5261 1132
Size: 97 1526 261 381 319 1545 2229
Fragments arranged by size:
2229 1545 1526 381 319 261 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6358
PfoI T'CCnGG_A
Cuts at: 2444 2821 3318 3510 2444
Size: 377 497 192 5292
Fragments arranged by size:
5292 497 377 192
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PmlI CAC'GTG
Cuts at: 2614 2614
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 3383 3415 4427 4459 5940 5972 2981
Size: 32 370 32 1012 32 1481 32 3367
Fragments arranged by size:
3367 1481 1012 370 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 3205 2097
Size: 1108 5250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PspOMI G'GGCC_C
Cuts at: 2036 2036
Size: 6358
PstI C_TGCA'G
Cuts at: 830 2730 3099 3183 3710 830
Size: 1900 369 84 527 3478
Fragments arranged by size:
3478 1900 527 369 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
PvuII CAG'CTG
Cuts at: 2170 2170
Size: 6358
SacI G_AGCT'C
Cuts at: 2319 2528 2319
Size: 209 6149
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SanDI GG'GwC_CC
Cuts at: 2097 2097
Size: 6358
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 4606 6318 4606
Size: 1712 4646
SfcI C'TryA_G
Cuts at: 826 1045 2726 3095 3179 3706 4841 5522 5713
Size: 219 1681 369 84 527 1135 681 191
Cuts at: 5713 826
Size: 1471
Fragments arranged by size:
1681 1471 1135 681 527 369 219 191
84
SfoI GGC'GCC
Cuts at: 3342 3342
Size: 6358
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 2419 3685 1872
Size: 547 1266 4545
Fragments arranged by size:
4545 1266 547
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4462 5363 5625 820
Size: 197 926 2519 901 262 1553
Fragments arranged by size:
2519 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3016 3716 1132
Size: 1884 700 3774
Fragments arranged by size:
3774 1884 700
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6358
StyI C'CwwG_G
Cuts at: 513 1065 2118 3797 513
Size: 552 1053 1679 3074
Fragments arranged by size:
3074 1679 1053 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4385 4402 4555 4740 5185 325
Size: 1255 2805 17 153 185 445 1498
Fragments arranged by size:
2805 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4604 6316 96
Size: 274 80 33 51 1443 2627 1712 138
Fragments arranged by size:
2627 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 6318 6350 2114
Size: 32 4172 32 2122
Fragments arranged by size:
4172 2122 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2689 2689
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 2417 3683 1870
Size: 547 1266 4545
Fragments arranged by size:
4545 1266 547
XmnI GAAnn'nnTTC
Cuts at: 2297 3329 4487 2297
Size: 1032 1158 4168
Fragments arranged by size:
4168 1158 1032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4166 276
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SexAI SfiI
SrfI SwaI