Restriction map for
pFN21AB4009
(Circular) (Six-base) MAPSORT of: pFN21AB4009.seq Check: 9022 from: 1 to: 7096
pFN21AB4009 7096 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2314 3504 2314
Size: 1190 5906
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3336 4906 278
Size: 53 83 186 936 1800 1570 2468
Fragments arranged by size:
2468 1800 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4068 4439 1937
Size: 2131 371 4594
Fragments arranged by size:
4594 2131 371
Acc65I G'GTAC_C
Cuts at: 4417 4417
Size: 7096
AclI AA'CG_TT
Cuts at: 1563 5223 5596 1563
Size: 3660 373 3063
Fragments arranged by size:
3660 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2351 3174 5159 6543 6811 1632
Size: 554 165 823 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 823 554 268 165
AfeI AGC'GCT
Cuts at: 3427 3427
Size: 7096
AflII C'TTAA_G
Cuts at: 820 1017 2260 820
Size: 197 1243 5656
Fragments arranged by size:
5656 1243 197
AflIII A'CryG_T
Cuts at: 1203 3207 5995 6972 1203
Size: 2004 2788 977 1327
Fragments arranged by size:
2788 2004 1327 977
AgeI A'CCGG_T
Cuts at: 4309 5927 4309
Size: 1618 5478
AhdI GACnn_n'nnGTC
Cuts at: 5825 5825
Size: 7096
AleI CACnn'nnGTG
Cuts at: 1166 3317 1166
Size: 2151 4945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7096
AlwNI CAG_nnn'CTG
Cuts at: 3490 4839 6411 3490
Size: 1349 1572 4175
Fragments arranged by size:
4175 1572 1349
ApaI G_GGCC'C
Cuts at: 4196 4196
Size: 7096
ApaLI G'TGCA_C
Cuts at: 5153 6309 5153
Size: 1156 5940
ApoI r'AATT_y
Cuts at: 1493 2726 4292 4405 4631 4783 6892 1493
Size: 1233 1566 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1566 1233 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 2709 2886 5650 160
Size: 872 1677 177 2764 1606
Fragments arranged by size:
2764 1677 1606 872 177
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7096
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2579 3735 3790 3839 4421 1870
Size: 73 253 383 1156 55 49 582 4545
Fragments arranged by size:
4545 1156 582 383 253 73 55 49
BamHI G'GATC_C
Cuts at: 4426 4793 4426
Size: 367 6729
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3446 3599 3730 4033 4183
Size: 325 217 627 1659 153 131 303 150
Cuts at: 4183 4226 4417 5872 618
Size: 43 191 1455 1842
Fragments arranged by size:
1842 1659 1455 627 325 303 217 191
153 150 131 43
BanII G_rGCy'C
Cuts at: 1391 2087 2197 2289 2619 2628 3840 4196 4415
Size: 696 110 92 330 9 1212 356 219
Cuts at: 4415 1391
Size: 4072
Fragments arranged by size:
4072 1212 696 356 330 219 110 92
9
BbeI G_GCGC'C
Cuts at: 3603 3603
Size: 7096
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3171 4089 928
Size: 971 1272 918 3935
Fragments arranged by size:
3935 1272 971 918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5287 5321 1903
Size: 34 3350 34 3678
Fragments arranged by size:
3678 3350 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 3279 4988 6198 3122
Size: 157 1709 1210 4020
Fragments arranged by size:
4020 1709 1210 157
BclI T'GATC_A
Cuts at: 1548 2386 1548
Size: 838 6258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3578 5707 136
Size: 107 122 71 631 605 1906 2129 1525
Fragments arranged by size:
2129 1906 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7091 7091
Size: 7096
BlpI GC'TnA_GC
Cuts at: 3893 4513 3893
Size: 620 6476
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4196 5157 6313 1790
Size: 1945 461 961 1156 2573
Fragments arranged by size:
2573 1945 1156 961 461
BmgBI CAC'GTC
Cuts at: 1318 4219 1318
Size: 2901 4195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 4827 5785
Size: 416 53 83 399 24 839 2571 958
Cuts at: 5785 442
Size: 1753
Fragments arranged by size:
2571 1753 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7096
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 7096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2116 2919 5756 1239
Size: 877 803 2837 2579
Fragments arranged by size:
2837 2579 877 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3809 5221 6086 6384 3809
Size: 1412 865 298 4521
Fragments arranged by size:
4521 1412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2209 3083 3217 3331 3520 5759 882
Size: 617 710 874 134 114 189 2239 2219
Fragments arranged by size:
2239 2219 874 710 617 189 134 114
BsaAI yAC'GTr
Cuts at: 493 1204 3013 4887 493
Size: 711 1809 1874 2702
Fragments arranged by size:
2702 1874 1809 711
BsaBI GATnn'nnATC
Cuts at: 7090 7090
Size: 7096
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3333 3600 3663 4903
Size: 53 83 186 936 1800 267 63 1240
Cuts at: 4903 5285 5920 275
Size: 382 635 1451
Fragments arranged by size:
1800 1451 1240 936 635 382 267 186
83 63 53
BsaWI w'CCGG_w
Cuts at: 3160 4309 5529 5927 6201 6348 6759 3160
Size: 1149 1220 398 274 147 411 3497
Fragments arranged by size:
3497 1220 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2403 2433 3599 3629 3986
Size: 30 165 30 1000 30 1166 30 357
Cuts at: 3986 4016 1178
Size: 30 4258
Fragments arranged by size:
4258 1166 1000 357 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 4165 1186
Size: 1111 1868 4117
Fragments arranged by size:
4117 1868 1111
BseYI C'CCAG_C
Cuts at: 4005 6299 6795 4005
Size: 2294 496 4306
Fragments arranged by size:
4306 2294 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3392 3476 3703 4165 1163
Size: 573 1656 84 227 462 4094
Fragments arranged by size:
4094 1656 573 462 227 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3744 4546 5307 6335 1540
Size: 275 186 1743 802 761 1028 2301
Fragments arranged by size:
2301 1743 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2289 2619 2628 2920 3245 3309 3987 4122 5157
Size: 330 9 292 325 64 678 135 1035
Cuts at: 5157 5242 6313 2289
Size: 85 1071 3072
Fragments arranged by size:
3072 1071 1035 678 330 325 292 135
85 64 9
BsmI GAATG_Cn'
Cuts at: 4607 4700 4607
Size: 93 7003
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2197 2289 2619 2628 2920 3245
Size: 399 297 110 92 330 9 292 325
Cuts at: 3245 3309 3735 3840 3987 4122 4196 4415 5157
Size: 64 426 105 147 135 74 219 742
Cuts at: 5157 5242 6313 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 330 325 297
292 219 147 135 110 105 92 85
74 64 9
BspEI T'CCGG_A
Cuts at: 3160 6759 3160
Size: 3599 3497
BspHI T'CATG_A
Cuts at: 4985 6715 4985
Size: 1730 5366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 3498 3504 4023 4451 844
Size: 1470 1184 6 519 428 3489
Fragments arranged by size:
3489 1470 1184 519 428 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2127 4021 4224 4983 1394
Size: 429 304 1894 203 759 3507
Fragments arranged by size:
3507 1894 759 429 304 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 5591 5765 65
Size: 1183 4343 174 1396
Fragments arranged by size:
4343 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4036 4309 5740 5927 1700
Size: 110 2226 273 1431 187 2869
Fragments arranged by size:
2869 2226 1431 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7096
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7096
BssSI C'ACGA_G
Cuts at: 5156 6168 5156
Size: 1012 6084
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 794
Size: 1253 5843
BstBI TT'CG_AA
Cuts at: 4372 5900 4372
Size: 1528 5568
BstEII G'GTnAC_C
Cuts at: 1184 3760 1184
Size: 2576 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 3772 3772
Size: 7096
BstYI r'GATC_y
Cuts at: 1972 4426 4793 5178 5195 6647 6733 6745 7091
Size: 2454 367 385 17 1452 86 12 346
Cuts at: 7091 1972
Size: 1977
Fragments arranged by size:
2454 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3551 4026 4188 3551
Size: 475 162 6459
Fragments arranged by size:
6459 475 162
BtgI C'CryG_G
Cuts at: 513 1065 2004 3093 3610 3831 513
Size: 552 939 1089 517 221 3778
Fragments arranged by size:
3778 1089 939 552 517 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6357
Fragments arranged by size:
6357 683 56
BtsI GCAGTG_nn'
Cuts at: 3424 4612 5406 5426 3424
Size: 1188 794 20 5094
Fragments arranged by size:
5094 1188 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7096
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7061
DraI TTT'AAA
Cuts at: 4400 4400
Size: 7096
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6103 809
Size: 507 4787 1802
Fragments arranged by size:
4787 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 3741 3909
Size: 54 1425 50 275 18 1705 206 168
Cuts at: 3909 3963 4543 5432 7061 8
Size: 54 580 889 1629 43
Fragments arranged by size:
1705 1629 1425 889 580 275 206 168
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3741 4543 1537
Size: 275 1929 802 4090
Fragments arranged by size:
4090 1929 802 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2607 4109 4115 5026 1353
Size: 1254 1502 6 911 3423
Fragments arranged by size:
3423 1502 1254 911 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5683 6057 6203 387
Size: 1064 138 4094 374 146 1280
Fragments arranged by size:
4094 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2287 2617 2626 2287
Size: 330 9 6757
Fragments arranged by size:
6757 330 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2186 2351 2919 3174 5159 5756
Size: 393 484 70 165 568 255 1985 597
Cuts at: 5756 6543 6811 1239
Size: 787 268 1524
Fragments arranged by size:
1985 1524 787 597 568 484 393 268
255 165 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2686 2686
Size: 7096
EcoO109I rG'GnC_Cy
Cuts at: 2155 2658 3487 3571 4192 4193 4233 2155
Size: 503 829 84 621 1 40 5018
Fragments arranged by size:
5018 829 621 503 84 40 1
EcoRI G'AATT_C
Cuts at: 4292 4405 4783 4292
Size: 113 378 6605
Fragments arranged by size:
6605 378 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 4213 2066
Size: 2147 4949
FspI TGC'GCA
Cuts at: 3878 5602 3878
Size: 1724 5372
FspAI rTGC'GCAy
Cuts at: 3878 3878
Size: 7096
HaeII r_GCGC'y
Cuts at: 1678 2603 3429 3603 3641 6243 1678
Size: 925 826 174 38 2602 2531
Fragments arranged by size:
2602 2531 925 826 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2642 2674 2798
Size: 32 135 32 439 32 599 32 124
Cuts at: 2798 2830 3110 3142 3425 3457 3599 3631 3773
Size: 32 280 32 283 32 142 32 142
Cuts at: 3773 3805 3986 4018 5737 5769 5811 5843 1373
Size: 32 181 32 1719 32 42 32 2626
Fragments arranged by size:
2626 1719 599 439 283 280 181 142
142 135 124 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3463 4440 4686 669
Size: 988 281 1525 977 246 3079
Fragments arranged by size:
3079 1525 988 977 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7096
HpaI GTT'AAC
Cuts at: 4686 4686
Size: 7096
KasI G'GCGC_C
Cuts at: 3599 3599
Size: 7096
KpnI G_GTAC'C
Cuts at: 4421 4421
Size: 7096
MfeI C'AATT_G
Cuts at: 4695 4695
Size: 7096
MluI A'CGCG_T
Cuts at: 6972 6972
Size: 7096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3192 6210 6394 1305
Size: 1887 3018 184 2007
Fragments arranged by size:
3018 2007 1887 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5274
Fragments arranged by size:
5274 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2546 3317 5054 5413 5572 518
Size: 648 1380 771 1737 359 159 2042
Fragments arranged by size:
2042 1737 1380 771 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 3612 3697 3820 4512 5189 6337 6582
Size: 627 1379 85 123 692 677 1148 245
Cuts at: 6582 1606
Size: 2120
Fragments arranged by size:
2120 1379 1148 692 677 627 245 123
85
NarI GG'CG_CC
Cuts at: 3600 3600
Size: 7096
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5605
Fragments arranged by size:
5605 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7096
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7096
NotI GC'GGCC_GC
Cuts at: 4543 4543
Size: 7096
NspI r_CATG'y
Cuts at: 1132 1229 4454 5999 1132
Size: 97 3225 1545 2229
Fragments arranged by size:
3225 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7096
PciI A'CATG_T
Cuts at: 5995 5995
Size: 7096
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3211 1108
Size: 2103 4993
PfoI T'CCnGG_A
Cuts at: 2619 2619
Size: 7096
PmeI GTTT'AAAC
Cuts at: 4400 4400
Size: 7096
PmlI CAC'GTG
Cuts at: 3013 3013
Size: 7096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5165 5197 6678 6710 5165
Size: 32 1481 32 5551
Fragments arranged by size:
5551 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2658 3487 4233 2155
Size: 503 829 746 5018
Fragments arranged by size:
5018 829 746 503
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7096
PsiI TTA'TAA
Cuts at: 4666 4666
Size: 7096
PspOMI G'GGCC_C
Cuts at: 4192 4192
Size: 7096
PstI C_TGCA'G
Cuts at: 830 3579 3702 4448 830
Size: 2749 123 746 3478
Fragments arranged by size:
3478 2749 746 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7096
PvuII CAG'CTG
Cuts at: 2233 3820 2233
Size: 1587 5509
SacI G_AGCT'C
Cuts at: 2289 2619 2628 2289
Size: 330 9 6757
Fragments arranged by size:
6757 330 9
SacII CC_GC'GG
Cuts at: 3613 3613
Size: 7096
SalI G'TCGA_C
Cuts at: 1936 4438 1936
Size: 2502 4594
SanDI GG'GwC_CC
Cuts at: 2658 4233 2658
Size: 1575 5521
SapI GCTCTTCn'nnn_
Cuts at: 2607 4109 2607
Size: 1502 5594
SbfI CC_TGCA'GG
Cuts at: 3579 4448 3579
Size: 869 6227
ScaI AGT'ACT
Cuts at: 5344 7056 5344
Size: 1712 5384
SexAI A'CCwGG_T
Cuts at: 3756 3756
Size: 7096
SfcI C'TryA_G
Cuts at: 826 1045 2997 3429 3575 3698 4444 5579 6260
Size: 219 1952 432 146 123 746 1135 681
Cuts at: 6260 6451 826
Size: 191 1471
Fragments arranged by size:
1952 1471 1135 746 681 432 219 191
146 123
SfoI GGC'GCC
Cuts at: 3601 3601
Size: 7096
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7096
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7096
SmaI CCC'GGG
Cuts at: 1872 3737 3841 4423 1872
Size: 1865 104 582 4545
Fragments arranged by size:
4545 1865 582 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2260 3824 5200 6101 6363 820
Size: 197 926 317 1564 1376 901 262 1553
Fragments arranged by size:
1564 1553 1376 926 901 317 262 197
SnaBI TAC'GTA
Cuts at: 493 4887 493
Size: 4394 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7096
SphI G_CATG'C
Cuts at: 1132 4454 1132
Size: 3322 3774
SrfI GCCC'GGGC
Cuts at: 3841 3841
Size: 7096
SspI AAT'ATT
Cuts at: 5 52 5020 5
Size: 47 4968 2081
Fragments arranged by size:
4968 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7096
StyI C'CwwG_G
Cuts at: 513 1065 2004 2014 2682 2773 3765 4535 513
Size: 552 939 10 668 91 992 770 3074
Fragments arranged by size:
3074 992 939 770 668 552 91 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3374 5123 5140 5293 5478 5923 325
Size: 1255 1794 1749 17 153 185 445 1498
Fragments arranged by size:
1794 1749 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 5342 7054
Size: 274 80 33 51 1443 1275 2090 1712
Cuts at: 7054 96
Size: 138
Fragments arranged by size:
2090 1712 1443 1275 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4336 4368 7056 7088 4336
Size: 32 2688 32 4344
Fragments arranged by size:
4344 2688 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7096
XbaI T'CTAG_A
Cuts at: 4432 4432
Size: 7096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3919 3919
Size: 7096
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7096
XmaI C'CCGG_G
Cuts at: 1870 3735 3839 4421 1870
Size: 1865 104 582 4545
Fragments arranged by size:
4545 1865 582 104
XmnI GAAnn'nnTTC
Cuts at: 2865 5225 2865
Size: 2360 4736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3334 4904 276
Size: 53 83 186 936 1800 1570 2468
Fragments arranged by size:
2468 1800 1570 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoRV FseI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SfiI SwaI