Restriction map for
pFN21AB3990
(Circular) (Six-base) MAPSORT of: pFN21AB3990.seq Check: 4465 from: 1 to: 7879
pFN21AB3990 7879 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3649 4059 4196 3649
Size: 410 137 7332
Fragments arranged by size:
7332 410 137
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5689 278
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5222 1937
Size: 3285 4594
Acc65I G'GTAC_C
Cuts at: 3790 5200 3790
Size: 1410 6469
AclI AA'CG_TT
Cuts at: 1563 6006 6379 1563
Size: 4443 373 3063
Fragments arranged by size:
4443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 3803 4814 4940 5942 7326 7594 1632
Size: 1958 213 1011 126 1002 1384 268 1917
Fragments arranged by size:
1958 1917 1384 1011 1002 268 213 126
AflII C'TTAA_G
Cuts at: 820 1017 2263 820
Size: 197 1246 6436
Fragments arranged by size:
6436 1246 197
AflIII A'CryG_T
Cuts at: 1203 2709 3062 6778 7755 1203
Size: 1506 353 3716 977 1327
Fragments arranged by size:
3716 1506 1327 977 353
AgeI A'CCGG_T
Cuts at: 6710 6710
Size: 7879
AhdI GACnn_n'nnGTC
Cuts at: 6608 6608
Size: 7879
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7879
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3929 3961 3972 4004 3929
Size: 32 11 32 7804
Fragments arranged by size:
7804 32 32 11
AlwNI CAG_nnn'CTG
Cuts at: 2632 3441 3586 4291 5622 7194 2632
Size: 809 145 705 1331 1572 3317
Fragments arranged by size:
3317 1572 1331 809 705 145
ApaI G_GGCC'C
Cuts at: 2803 3475 2803
Size: 672 7207
ApaLI G'TGCA_C
Cuts at: 2726 5936 7092 2726
Size: 3210 1156 3513
Fragments arranged by size:
3513 3210 1156
ApoI r'AATT_y
Cuts at: 1493 5077 5188 5414 5566 7675 1493
Size: 3584 111 226 152 2109 1697
Fragments arranged by size:
3584 2109 1697 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 6433 160
Size: 872 5401 1606
Fragments arranged by size:
5401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
AvaI C'yCGr_G
Cuts at: 1870 1943 2759 4039 4600 5204 1870
Size: 73 816 1280 561 604 4545
Fragments arranged by size:
4545 1280 816 604 561 73
BamHI G'GATC_C
Cuts at: 2895 5209 5576 2895
Size: 2314 367 5198
Fragments arranged by size:
5198 2314 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 2840 3011 3125 3790
Size: 325 217 627 959 94 171 114 665
Cuts at: 3790 4072 5200 6655 618
Size: 282 1128 1455 1842
Fragments arranged by size:
1842 1455 1128 959 665 627 325 282
217 171 114 94
BanII G_rGCy'C
Cuts at: 1391 2364 2803 2937 3102 3251 3324 3433 3475
Size: 973 439 134 165 149 73 109 42
Cuts at: 3475 4135 5198 1391
Size: 660 1063 4072
Fragments arranged by size:
4072 1063 973 660 439 165 149 134
109 73 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4104 928
Size: 971 2205 4703
Fragments arranged by size:
4703 2205 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6070 6104 1903
Size: 34 4133 34 3678
Fragments arranged by size:
4133 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5771 6981 5771
Size: 1210 6669
BclI T'GATC_A
Cuts at: 1548 4842 1548
Size: 3294 4585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3074 4060 4300
Size: 107 122 71 631 605 1402 986 240
Cuts at: 4300 4715 6490 136
Size: 415 1775 1525
Fragments arranged by size:
1775 1525 1402 986 631 605 415 240
122 107 71
BglII A'GATC_T
Cuts at: 7874 7874
Size: 7879
BlpI GC'TnA_GC
Cuts at: 3318 4268 5296 3318
Size: 950 1028 5901
Fragments arranged by size:
5901 1028 950
Bme1580I G_kGCm'C
Cuts at: 1790 2353 2730 2751 2803 2828 2890 3070 3475
Size: 563 377 21 52 25 62 180 405
Cuts at: 3475 5940 7096 1790
Size: 2465 1156 2573
Fragments arranged by size:
2573 2465 1156 563 405 377 180 62
52 25 21
BmgBI CAC'GTC
Cuts at: 1318 3063 4102 4915 1318
Size: 1745 1039 813 4282
Fragments arranged by size:
4282 1745 1039 813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5610 6568 442
Size: 416 53 83 399 24 4193 958 1753
Fragments arranged by size:
4193 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4699 4699
Size: 7879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2522 2946 3159 3620 4166 4561 4682
Size: 956 327 424 213 461 546 395 121
Cuts at: 4682 4805 6539 1239
Size: 123 1734 2579
Fragments arranged by size:
2579 1734 956 546 461 424 395 327
213 123 121
Bpu10I CC'TnA_GC
Cuts at: 4056 5132 4056
Size: 1076 6803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2091 2190 3434 3632 3843 6004 6869 7167 2091
Size: 99 1244 198 211 2161 865 298 2803
Fragments arranged by size:
2803 2161 1244 865 298 211 198 99
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 6542 882
Size: 617 2976 2067 2219
Fragments arranged by size:
2976 2219 2067 617
BsaAI yAC'GTr
Cuts at: 493 1204 2878 5670 493
Size: 711 1674 2792 2702
Fragments arranged by size:
2792 2702 1674 711
BsaBI GATnn'nnATC
Cuts at: 3897 7873 3897
Size: 3976 3903
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2646 3231 4887 5686
Size: 53 83 186 936 1113 585 1656 799
Cuts at: 5686 6068 6703 275
Size: 382 635 1451
Fragments arranged by size:
1656 1451 1113 936 799 635 585 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5088 6312 6710 6984 7131 7542 5088
Size: 1224 398 274 147 411 5425
Fragments arranged by size:
5425 1224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3972 4002 4427 4457 1178
Size: 30 165 30 2569 30 425 30 4600
Fragments arranged by size:
4600 2569 425 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2498 3557 3902 4163 4451 4454 4510
Size: 837 475 1059 345 261 288 3 56
Cuts at: 4510 4564 1186
Size: 54 4501
Fragments arranged by size:
4501 1059 837 475 345 288 261 56
54 3
BseYI C'CCAG_C
Cuts at: 2318 2868 4339 7082 7578 2318
Size: 550 1471 2743 496 2619
Fragments arranged by size:
2743 2619 1471 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3166 4834 1163
Size: 573 1430 1668 4208
Fragments arranged by size:
4208 1668 1430 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2445 2866 3342 5329 6090 7118
Size: 275 186 444 421 476 1987 761 1028
Cuts at: 7118 1540
Size: 2301
Fragments arranged by size:
2301 1987 1028 761 476 444 421 275
186
BsiHKAI G_wGCw'C
Cuts at: 2730 3251 4415 4935 5940 6025 7096 2730
Size: 521 1164 520 1005 85 1071 3513
Fragments arranged by size:
3513 1164 1071 1005 521 520 85
BsmI GAATG_Cn'
Cuts at: 3050 5390 5483 3050
Size: 2340 93 5446
Fragments arranged by size:
5446 2340 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2353 2364 2730 2751 2803 2828 2890
Size: 399 563 11 366 21 52 25 62
Cuts at: 2890 2937 3070 3102 3251 3324 3433 3475 4135
Size: 47 133 32 149 73 109 42 660
Cuts at: 4135 4415 4935 5198 5940 6025 7096 1391
Size: 280 520 263 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 563 520 399 366
280 263 149 133 109 85 73 62
52 47 42 32 25 21 11
BspEI T'CCGG_A
Cuts at: 5088 7542 5088
Size: 2454 5425
BspHI T'CATG_A
Cuts at: 5768 7498 5768
Size: 1730 6149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3649 3664 4059 4196 4457 5234 844
Size: 2805 15 395 137 261 777 3489
Fragments arranged by size:
3489 2805 777 395 261 137 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2248 4060 4591 5766 1394
Size: 429 425 1812 531 1175 3507
Fragments arranged by size:
3507 1812 1175 531 429 425
BsrDI GCAATG_nn'
Cuts at: 65 1248 6374 6548 65
Size: 1183 5126 174 1396
Fragments arranged by size:
5126 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 4636 6523 6710 1700
Size: 110 525 2301 1887 187 2869
Fragments arranged by size:
2869 2301 1887 525 187 110
BsrGI T'GTAC_A
Cuts at: 96 3084 96
Size: 2988 4891
BssHII G'CGCG_C
Cuts at: 1925 3604 1925
Size: 1679 6200
BssSI C'ACGA_G
Cuts at: 5939 6951 5939
Size: 1012 6867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3024 3586 794
Size: 2230 562 5087
Fragments arranged by size:
5087 2230 562
BstBI TT'CG_AA
Cuts at: 4320 6683 4320
Size: 2363 5516
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7879
BstXI CCAn_nnnn'nTGG
Cuts at: 2970 3077 2970
Size: 107 7772
BstYI r'GATC_y
Cuts at: 1972 2895 4755 5209 5576 5961 5978 7430 7516
Size: 923 1860 454 367 385 17 1452 86
Cuts at: 7516 7528 7874 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1860 1452 923 454 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2326 3681 3822 3957 4076 4296 5143
Size: 552 1261 1355 141 135 119 220 847
Cuts at: 5143 513
Size: 3249
Fragments arranged by size:
3249 1355 1261 847 552 220 141 135
119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7140
Fragments arranged by size:
7140 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 2199 2596 2782 4678 5006 5395 6189 6209
Size: 128 397 186 1896 328 389 794 20
Cuts at: 6209 2071
Size: 3741
Fragments arranged by size:
3741 1896 794 397 389 328 186 128
20
ClaI AT'CG_AT
Cuts at: 1554 2940 1554
Size: 1386 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2818 2853 529
Size: 35 2254 35 5555
Fragments arranged by size:
5555 2254 35 35
DraI TTT'AAA
Cuts at: 5183 5183
Size: 7879
DraIII CAC_nnn'GTG
Cuts at: 2881 2881
Size: 7879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6886 809
Size: 507 5570 1802
Fragments arranged by size:
5570 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 2442 2721
Size: 54 1425 50 275 18 513 99 279
Cuts at: 2721 3396 4716 4738 4873 5326 6215 7844 8
Size: 675 1320 22 135 453 889 1629 43
Fragments arranged by size:
1629 1425 1320 889 675 513 453 279
275 135 99 54 50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2442 5326 1537
Size: 275 630 2884 4090
Fragments arranged by size:
4090 2884 630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2705 4026 4317 4389 5809 1353
Size: 1352 1321 291 72 1420 3423
Fragments arranged by size:
3423 1420 1352 1321 291 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2386 6466 6840 6986 387
Size: 1064 138 797 4080 374 146 1280
Fragments arranged by size:
4080 1280 1064 797 374 146 138
EcoICRI GAG'CTC
Cuts at: 3249 3249
Size: 7879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2522 2946 3159 3590 3620 3803
Size: 393 563 327 424 213 431 30 183
Cuts at: 3803 4166 4561 4682 4805 4814 4940 5942 6539
Size: 363 395 121 123 9 126 1002 597
Cuts at: 6539 7326 7594 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1002 787 597 563 431 424 395
393 363 327 268 213 183 126 123
121 30 9
EcoO109I rG'GnC_Cy
Cuts at: 2311 3471 3472 3748 2311
Size: 1160 1 276 6442
Fragments arranged by size:
6442 1160 276 1
EcoRI G'AATT_C
Cuts at: 5077 5188 5566 5077
Size: 111 378 7390
Fragments arranged by size:
7390 378 111
FspI TGC'GCA
Cuts at: 2372 3632 3768 6385 2372
Size: 1260 136 2617 3866
Fragments arranged by size:
3866 2617 1260 136
FspAI rTGC'GCAy
Cuts at: 2372 2372
Size: 7879
HaeII r_GCGC'y
Cuts at: 1678 2146 2325 4459 7026 1678
Size: 468 179 2134 2567 2531
Fragments arranged by size:
2567 2531 2134 468 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2089 2121 2576 2608 2612
Size: 32 135 32 517 32 455 32 4
Cuts at: 2612 2644 3373 3405 4427 4459 6520 6552 6594
Size: 32 729 32 1022 32 2061 32 42
Cuts at: 6594 6626 1373
Size: 32 2626
Fragments arranged by size:
2626 2061 1022 729 517 455 135 42
32 32 32 32 32 32 32 32
32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 5223 5469 669
Size: 988 281 3285 246 3079
Fragments arranged by size:
3285 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7879
HpaI GTT'AAC
Cuts at: 5469 5469
Size: 7879
KpnI G_GTAC'C
Cuts at: 3794 5204 3794
Size: 1410 6469
MfeI C'AATT_G
Cuts at: 5478 5478
Size: 7879
MluI A'CGCG_T
Cuts at: 7755 7755
Size: 7879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4356 6993 7177 1305
Size: 3051 2637 184 2007
Fragments arranged by size:
3051 2637 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3398 4875 10
Size: 54 1425 343 1566 1477 3014
Fragments arranged by size:
3014 1566 1477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 3092 5837 6196 6355 518
Size: 648 1497 429 2745 359 159 2042
Fragments arranged by size:
2745 2042 1497 648 429 359 159
MspA1I CmG'CkG
Cuts at: 1606 2868 3406 3583 3703 3736 3959 4078 4156
Size: 1262 538 177 120 33 223 119 78
Cuts at: 4156 4206 4818 5295 5972 7120 7365 1606
Size: 50 612 477 677 1148 245 2120
Fragments arranged by size:
2120 1262 1148 677 612 538 477 245
223 177 120 119 78 50 33
NaeI GCC'GGC
Cuts at: 2337 2337
Size: 7879
NcoI C'CATG_G
Cuts at: 513 1065 3681 3822 513
Size: 552 2616 141 4570
Fragments arranged by size:
4570 2616 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7879
NgoMIV G'CCGG_C
Cuts at: 2335 2335
Size: 7879
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7879
NotI GC'GGCC_GC
Cuts at: 5326 5326
Size: 7879
NspI r_CATG'y
Cuts at: 1132 1229 2560 2713 2985 4177 4419 4753 4939
Size: 97 1331 153 272 1192 242 334 186
Cuts at: 4939 5237 6782 1132
Size: 298 1545 2229
Fragments arranged by size:
2229 1545 1331 1192 334 298 272 242
186 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7879
PciI A'CATG_T
Cuts at: 2709 6778 2709
Size: 4069 3810
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7879
PfoI T'CCnGG_A
Cuts at: 2355 3461 4211 2355
Size: 1106 750 6023
Fragments arranged by size:
6023 1106 750
PmeI GTTT'AAAC
Cuts at: 5183 5183
Size: 7879
PmlI CAC'GTG
Cuts at: 2878 2878
Size: 7879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3972 4004 4946 4978 5948 5980 7461 7493 3972
Size: 32 942 32 970 32 1481 32 4358
Fragments arranged by size:
4358 1481 970 942 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3748 3748
Size: 7879
PshAI GACnn'nnGTC
Cuts at: 1613 4467 1613
Size: 2854 5025
PsiI TTA'TAA
Cuts at: 5449 5449
Size: 7879
PspOMI G'GGCC_C
Cuts at: 2799 3471 2799
Size: 672 7207
PspXI vC'TCGA_Gb
Cuts at: 4600 4600
Size: 7879
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2411 2443 2411
Size: 32 7847
PstI C_TGCA'G
Cuts at: 830 2041 2793 3495 3507 4392 5231 830
Size: 1211 752 702 12 885 839 3478
Fragments arranged by size:
3478 1211 885 839 752 702 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7879
PvuII CAG'CTG
Cuts at: 3736 4156 3736
Size: 420 7459
SacI G_AGCT'C
Cuts at: 3251 3251
Size: 7879
SacII CC_GC'GG
Cuts at: 3960 4079 3960
Size: 119 7760
SalI G'TCGA_C
Cuts at: 1936 5221 1936
Size: 3285 4594
SbfI CC_TGCA'GG
Cuts at: 5231 5231
Size: 7879
ScaI AGT'ACT
Cuts at: 3575 6127 7839 3575
Size: 2552 1712 3615
Fragments arranged by size:
3615 2552 1712
SfcI C'TryA_G
Cuts at: 826 1045 2037 2789 3491 3503 3949 4388 5046
Size: 219 992 752 702 12 446 439 658
Cuts at: 5046 5227 6362 7043 7234 826
Size: 181 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 752 702 681 658 446
439 219 191 181 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7879
SmaI CCC'GGG
Cuts at: 1872 5206 1872
Size: 3334 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2106 2205 2263 3449 3647 3858
Size: 197 926 163 99 58 1186 198 211
Cuts at: 3858 4600 5983 6884 7146 820
Size: 742 1383 901 262 1553
Fragments arranged by size:
1553 1383 1186 926 901 742 262 211
198 197 163 99 58
SnaBI TAC'GTA
Cuts at: 493 5670 493
Size: 5177 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7879
SphI G_CATG'C
Cuts at: 1132 5237 1132
Size: 4105 3774
SspI AAT'ATT
Cuts at: 5 52 3202 5803 5
Size: 47 3150 2601 2081
Fragments arranged by size:
3150 2601 2081 47
StuI AGG'CCT
Cuts at: 1517 2410 3193 4037 1517
Size: 893 783 844 5359
Fragments arranged by size:
5359 893 844 783
StyI C'CwwG_G
Cuts at: 513 1065 2364 3276 3681 3822 4394 4710 5029
Size: 552 1299 912 405 141 572 316 319
Cuts at: 5029 5318 513
Size: 289 3074
Fragments arranged by size:
3074 1299 912 572 552 405 319 316
289 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2999 5906 5923 6076 6261 6706 325
Size: 1255 1419 2907 17 153 185 445 1498
Fragments arranged by size:
2907 1498 1419 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 2418 3084
Size: 274 80 33 51 1443 405 36 666
Cuts at: 3084 3573 3924 6125 7837 96
Size: 489 351 2201 1712 138
Fragments arranged by size:
2201 1712 1443 666 489 405 351 274
138 80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2636 2660 2668 2692 7839 7871 2636
Size: 24 8 24 5147 32 2644
Fragments arranged by size:
5147 2644 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2022 2534 4945 1316
Size: 706 512 2411 4250
Fragments arranged by size:
4250 2411 706 512
XbaI T'CTAG_A
Cuts at: 5215 5215
Size: 7879
XhoI C'TCGA_G
Cuts at: 1943 4600 1943
Size: 2657 5222
XmaI C'CCGG_G
Cuts at: 1870 5204 1870
Size: 3334 4545
XmnI GAAnn'nnTTC
Cuts at: 4365 4961 6008 4365
Size: 596 1047 6236
Fragments arranged by size:
6236 1047 596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5687 276
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI KasI
NarI NruI NsiI PacI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI