Restriction map for
pFN21AB3987
(Circular) (Six-base) MAPSORT of: pFN21AB3987.seq Check: 2027 from: 1 to: 5848
pFN21AB3987 5848 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2985 3658 278
Size: 53 83 186 936 1449 673 2468
Fragments arranged by size:
2468 1449 936 673 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2938 3149 3911 5295 5563 1632
Size: 1306 211 762 1384 268 1917
Fragments arranged by size:
1917 1384 1306 762 268 211
AfeI AGC'GCT
Cuts at: 2990 2990
Size: 5848
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 2034 3591 5163 2034
Size: 1557 1572 2719
Fragments arranged by size:
2719 1572 1557
ApaI G_GGCC'C
Cuts at: 2065 2065
Size: 5848
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 3157 3383 3535 5644 1493
Size: 1664 226 152 2109 1697
Fragments arranged by size:
2109 1697 1664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 3169 4624 618
Size: 325 217 627 382 1000 1455 1842
Fragments arranged by size:
1842 1455 1000 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2065 2318 3167 1391
Size: 674 253 849 4072
Fragments arranged by size:
4072 849 674 253
BbeI G_GCGC'C
Cuts at: 2173 2173
Size: 5848
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2927 928
Size: 971 1028 3849
Fragments arranged by size:
3849 1028 971
BbvCI CC'TCA_GC
Cuts at: 2027 2027
Size: 5848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 4459 136
Size: 107 122 71 631 605 388 2399 1525
Fragments arranged by size:
2399 1525 631 605 388 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2065 3909 5065 1790
Size: 275 1844 1156 2573
Fragments arranged by size:
2573 1844 1156 275
BmgBI CAC'GTC
Cuts at: 1318 2742 1318
Size: 1424 4424
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2497 2749 2996 4508 1239
Size: 1058 200 252 247 1512 2579
Fragments arranged by size:
2579 1512 1058 252 247 200
Bpu10I CC'TnA_GC
Cuts at: 2027 2465 2027
Size: 438 5410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2294 2302 3973 4838 5136 2210
Size: 84 8 1671 865 298 2922
Fragments arranged by size:
2922 1671 865 298 84 8
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2764 2831 4511 882
Size: 617 1265 67 1680 2219
Fragments arranged by size:
2219 1680 1265 617 67
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 2237 5842 2237
Size: 3605 2243
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2170 2982 3655 4037
Size: 53 83 186 936 637 812 673 382
Cuts at: 4037 4672 275
Size: 635 1451
Fragments arranged by size:
1451 936 812 673 637 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2565 4281 4679 4953 5100 5511 2565
Size: 1716 398 274 147 411 2902
Fragments arranged by size:
2902 1716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2752 1186
Size: 1566 4282
BseYI C'CCAG_C
Cuts at: 2191 2527 2703 5051 5547 2191
Size: 336 176 2348 496 2492
Fragments arranged by size:
2492 2348 496 336 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2251 3298 4059 5087 1540
Size: 275 186 48 202 1047 761 1028 2301
Fragments arranged by size:
2301 1047 1028 761 275 202 186 48
BsiHKAI G_wGCw'C
Cuts at: 3909 3994 5065 3909
Size: 85 1071 4692
Fragments arranged by size:
4692 1071 85
BsmI GAATG_Cn'
Cuts at: 2352 3359 3452 2352
Size: 1007 93 4748
Fragments arranged by size:
4748 1007 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2875 3013 2875
Size: 138 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2318 3167 3909 3994 5065 1391
Size: 399 275 253 849 742 85 1071 2174
Fragments arranged by size:
2174 1071 849 742 399 275 253 85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3136 3203 844
Size: 2292 67 3489
Fragments arranged by size:
3489 2292 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 2208 3735 1394
Size: 429 332 53 1527 3507
Fragments arranged by size:
3507 1527 429 332 53
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2054 2434 2808 4492 4679 1700
Size: 110 234 10 380 374 1684 187 2869
Fragments arranged by size:
2869 1684 380 374 234 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 2088 2090 2158 1925
Size: 163 2 68 5615
Fragments arranged by size:
5615 163 68 2
BssSI C'ACGA_G
Cuts at: 2337 3908 4920 2337
Size: 1571 1012 3265
Fragments arranged by size:
3265 1571 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2812
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2181 2664 3178 3545 3930 3947 5399 5485
Size: 209 483 514 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 514 483 385 367 346 209
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2245 513
Size: 552 1180 4116
Fragments arranged by size:
4116 1180 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 4158 4178 3364
Size: 794 20 5034
Fragments arranged by size:
5034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2248 2436
Size: 54 1425 50 275 18 216 202 188
Cuts at: 2436 2572 2692 3295 4184 5813 8
Size: 136 120 603 889 1629 43
Fragments arranged by size:
1629 1425 889 603 275 216 202 188
136 120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2248 3295 1537
Size: 275 234 202 1047 4090
Fragments arranged by size:
4090 1047 275 234 202
EarI CTCTTCn'nnn_
Cuts at: 1353 2864 2888 3011 3047 3778 1353
Size: 1511 24 123 36 731 3423
Fragments arranged by size:
3423 1511 731 123 36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2497 2749 2938 2996 3149 3911
Size: 393 665 200 252 189 58 153 762
Cuts at: 3911 4508 5295 5563 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 762 665 597 393 268 252
200 189 153 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2789 2789
Size: 5848
EcoO109I rG'GnC_Cy
Cuts at: 2061 2782 2061
Size: 721 5127
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
EcoRV GAT'ATC
Cuts at: 2605 2605
Size: 5848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2878 2959 3058 2686
Size: 192 81 99 5476
Fragments arranged by size:
5476 192 99 81
FspI TGC'GCA
Cuts at: 2237 4354 2237
Size: 2117 3731
FspAI rTGC'GCAy
Cuts at: 2237 2237
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2173 2273 2710 2992 4995 1678
Size: 495 100 437 282 2003 2531
Fragments arranged by size:
2531 2003 495 437 282 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373
Size: 32 135 32 2917 32 42 32 2626
Fragments arranged by size:
2917 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3192 3438 669
Size: 988 281 910 344 246 3079
Fragments arranged by size:
3079 988 910 344 281 246
HindIII A'AGCT_T
Cuts at: 748 2345 748
Size: 1597 4251
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KasI G'GCGC_C
Cuts at: 2169 2169
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3806 4165 4324 518
Size: 648 2640 359 159 2042
Fragments arranged by size:
2640 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2085 2449 2527 2578 2698 3264 3941
Size: 428 51 364 78 51 120 566 677
Cuts at: 3941 5089 5334 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 566 428 364 245 120
78 51 51
NaeI GCC'GGC
Cuts at: 2046 2056 2436 2046
Size: 10 380 5458
Fragments arranged by size:
5458 380 10
NarI GG'CG_CC
Cuts at: 2170 2170
Size: 5848
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5296
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NgoMIV G'CCGG_C
Cuts at: 2044 2054 2434 2044
Size: 10 380 5458
Fragments arranged by size:
5458 380 10
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 3206 4751 1132
Size: 97 1977 1545 2229
Fragments arranged by size:
2229 1977 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PfoI T'CCnGG_A
Cuts at: 2550 2946 2550
Size: 396 5452
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2782 2782
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 2843 1613
Size: 1230 4618
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PspOMI G'GGCC_C
Cuts at: 2061 2061
Size: 5848
PstI C_TGCA'G
Cuts at: 830 3120 3147 3200 830
Size: 2290 27 53 3478
Fragments arranged by size:
3478 2290 53 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2449 2527 2578 2449
Size: 78 51 5719
Fragments arranged by size:
5719 78 51
RsrII CG'GwC_CG
Cuts at: 2164 2164
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SapI GCTCTTCn'nnn_
Cuts at: 2864 2864
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3120 3200 3120
Size: 80 5768
ScaI AGT'ACT
Cuts at: 2546 4096 5808 2546
Size: 1550 1712 2586
Fragments arranged by size:
2586 1712 1550
SexAI A'CCwGG_T
Cuts at: 2778 2994 2778
Size: 216 5632
SfcI C'TryA_G
Cuts at: 826 1045 3116 3143 3196 4331 5012 5203 826
Size: 219 2071 27 53 1135 681 191 1471
Fragments arranged by size:
2071 1471 1135 681 219 191 53 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 2060 2060
Size: 5848
SfoI GGC'GCC
Cuts at: 2171 2171
Size: 5848
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2309 2317 3952 4853 5115
Size: 197 926 282 84 8 1635 901 262
Cuts at: 5115 820
Size: 1553
Fragments arranged by size:
1635 1553 926 901 282 262 197 84
8
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2357 2628 3287 513
Size: 552 1292 271 659 3074
Fragments arranged by size:
3074 1292 659 552 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 4094 5806
Size: 274 80 33 51 1443 567 1550 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1712 1550 1443 567 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 2780 1316
Size: 1464 4384
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2635 2752 2812 2011
Size: 624 117 60 5047
Fragments arranged by size:
5047 624 117 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 2546 2717 2831 2942 3977 2546
Size: 171 114 111 1035 4417
Fragments arranged by size:
4417 1035 171 114 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2983 3656 276
Size: 53 83 186 936 1449 673 2468
Fragments arranged by size:
2468 1449 936 673 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI FseI NruI NsiI PacI
PmlI PspXI PsrI SacI SacII SanDI SrfI SwaI