Restriction map for
pFN21AB3975
(Circular) (Six-base) MAPSORT of: pFN21AB3975.seq Check: 7631 from: 1 to: 6592
pFN21AB3975 6592 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3383 3383
Size: 6592
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4402 278
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2190 2961 3935 1937
Size: 253 771 974 4594
Fragments arranged by size:
4594 974 771 253
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 2926 4655 6039 6307 1632
Size: 920 374 1729 1384 268 1917
Fragments arranged by size:
1917 1729 1384 920 374 268
AfeI AGC'GCT
Cuts at: 2321 2333 2321
Size: 12 6580
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 2889 5491 6468 1203
Size: 1686 2602 977 1327
Fragments arranged by size:
2602 1686 1327 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 2893 3798 5321 2893
Size: 905 1523 4164
Fragments arranged by size:
4164 1523 905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2693 3783 1184
Size: 1509 1090 3993
Fragments arranged by size:
3993 1509 1090
AlwNI CAG_nnn'CTG
Cuts at: 2793 3223 3541 3749 4335 5907 2793
Size: 430 318 208 586 1572 3478
Fragments arranged by size:
3478 1572 586 430 318 208
ApaI G_GGCC'C
Cuts at: 2052 2218 3113 3162 3332 3482 3489 3625 2052
Size: 166 895 49 170 150 7 136 5019
Fragments arranged by size:
5019 895 170 166 150 136 49 7
ApaLI G'TGCA_C
Cuts at: 4649 5805 4649
Size: 1156 5436
ApoI r'AATT_y
Cuts at: 1493 3901 4127 4279 6388 1493
Size: 2408 226 152 2109 1697
Fragments arranged by size:
2408 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5146 160
Size: 872 4114 1606
Fragments arranged by size:
4114 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 3161 3917 1870
Size: 73 1170 48 756 4545
Fragments arranged by size:
4545 1170 756 73 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3637 3670 3637
Size: 33 6559
BamHI G'GATC_C
Cuts at: 3922 4289 3922
Size: 367 6225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 3022 3913 5368 618
Size: 325 217 627 437 798 891 1455 1842
Fragments arranged by size:
1842 1455 891 798 627 437 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2218 2703 2937 3113 3162 3252 3280
Size: 661 166 485 234 176 49 90 28
Cuts at: 3280 3332 3482 3489 3625 3911 1391
Size: 52 150 7 136 286 4072
Fragments arranged by size:
4072 661 485 286 234 176 166 150
136 90 52 49 28 7
BbeI G_GCGC'C
Cuts at: 2228 3026 2228
Size: 798 5794
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5621
BbvCI CC'TCA_GC
Cuts at: 2128 3772 2128
Size: 1644 4948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3870 3904 4783 4817 1903
Size: 34 1933 34 879 34 3678
Fragments arranged by size:
3678 1933 879 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4484 5694 4484
Size: 1210 5382
BclI T'GATC_A
Cuts at: 1548 2392 1548
Size: 844 5748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2098 2222 2802
Size: 107 122 71 631 605 426 124 580
Cuts at: 2802 3314 3336 3544 3711 5203 136
Size: 512 22 208 167 1492 1525
Fragments arranged by size:
1525 1492 631 605 580 512 426 208
167 124 122 107 71 22
BglII A'GATC_T
Cuts at: 6587 6587
Size: 6592
BlpI GC'TnA_GC
Cuts at: 2702 4009 2702
Size: 1307 5285
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2218 2867 3113 3162 3332 3482 3489
Size: 262 166 649 246 49 170 150 7
Cuts at: 3489 3570 3625 4653 5809 1790
Size: 81 55 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 649 262 246 170 166
150 81 55 49 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3619 4323 5281
Size: 416 53 83 399 24 2202 704 958
Cuts at: 5281 442
Size: 1753
Fragments arranged by size:
2202 1753 958 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2954
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2501 3260 3391 3406 3418 3435 3816
Size: 1109 153 759 131 15 12 17 381
Cuts at: 3816 5252 1239
Size: 1436 2579
Fragments arranged by size:
2579 1436 1109 759 381 153 131 17
15 12
Bpu10I CC'TnA_GC
Cuts at: 2128 2697 3772 2128
Size: 569 1075 4948
Fragments arranged by size:
4948 1075 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 4717 5582 5880 2708
Size: 2009 865 298 3420
Fragments arranged by size:
3420 2009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5255 882
Size: 617 3756 2219
Fragments arranged by size:
3756 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4383 493
Size: 711 3179 2702
Fragments arranged by size:
3179 2702 711
BsaBI GATnn'nnATC
Cuts at: 6586 6586
Size: 6592
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2225 2628 3023 4399
Size: 53 83 186 936 692 403 395 1376
Cuts at: 4399 4781 5416 275
Size: 382 635 1451
Fragments arranged by size:
1451 1376 936 692 635 403 395 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2550 2927 3444 3780 5025 5423 5697 5844 6255
Size: 377 517 336 1245 398 274 147 411
Cuts at: 6255 2550
Size: 2887
Fragments arranged by size:
2887 1245 517 411 398 377 336 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2498 2528 1178
Size: 30 165 30 1095 30 5242
Fragments arranged by size:
5242 1095 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3421 1186
Size: 2235 4357
BseYI C'CCAG_C
Cuts at: 2217 2989 3139 3534 3636 3742 3756 5795 6291
Size: 772 150 395 102 106 14 2039 496
Cuts at: 6291 2217
Size: 2518
Fragments arranged by size:
2518 2039 772 496 395 150 106 102
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2653 3167 1163
Size: 573 917 514 4588
Fragments arranged by size:
4588 917 573 514
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3006 3309 4042 4803 5831 1540
Size: 275 186 1005 303 733 761 1028 2301
Fragments arranged by size:
2301 1028 1005 761 733 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2703 2859 3878 4653 4738 5809 2061
Size: 642 156 1019 775 85 1071 2844
Fragments arranged by size:
2844 1071 1019 775 642 156 85
BsmI GAATG_Cn'
Cuts at: 4103 4196 4103
Size: 93 6499
BsmBI CGTCTCn'nnnn_
Cuts at: 2635 2635
Size: 6592
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2061 2218 2703 2859 2867 2937
Size: 399 262 9 157 485 156 8 70
Cuts at: 2937 3113 3162 3252 3280 3332 3482 3489 3570
Size: 176 49 90 28 52 150 7 81
Cuts at: 3570 3625 3878 3911 4653 4738 5809 1391
Size: 55 253 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 262 253 176
157 156 150 90 85 81 70 55
52 49 33 28 9 8 7
BspEI T'CCGG_A
Cuts at: 2550 3780 6255 2550
Size: 1230 2475 2887
Fragments arranged by size:
2887 2475 1230
BspHI T'CATG_A
Cuts at: 4481 6211 4481
Size: 1730 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 3383 3947 844
Size: 1212 1327 564 3489
Fragments arranged by size:
3489 1327 1212 564
BsrBI CCG'CTC
Cuts at: 1394 1823 2844 4479 1394
Size: 429 1021 1635 3507
Fragments arranged by size:
3507 1635 1021 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5087 5261 65
Size: 1183 3839 174 1396
Fragments arranged by size:
3839 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3001 3168 5236 5423 1700
Size: 110 1191 167 2068 187 2869
Fragments arranged by size:
2869 2068 1191 187 167 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2071 2793 3136 794
Size: 1277 722 343 4250
Fragments arranged by size:
4250 1277 722 343
BstBI TT'CG_AA
Cuts at: 5396 5396
Size: 6592
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6592
BstYI r'GATC_y
Cuts at: 1972 2811 3922 4289 4674 4691 6143 6229 6241
Size: 839 1111 367 385 17 1452 86 12
Cuts at: 6241 6587 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1111 839 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2191 2191
Size: 6592
Bsu36I CC'TnA_GG
Cuts at: 2850 2850
Size: 6592
BtgI C'CryG_G
Cuts at: 513 1065 2004 2023 2272 2623 3859 513
Size: 552 939 19 249 351 1236 3246
Fragments arranged by size:
3246 1236 939 552 351 249 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5853
Fragments arranged by size:
5853 683 56
BtsI GCAGTG_nn'
Cuts at: 2317 2675 2713 3349 3605 4108 4902 4922 2317
Size: 358 38 636 256 503 794 20 3987
Fragments arranged by size:
3987 794 636 503 358 256 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2371 2415
Size: 54 1425 50 275 18 177 364 44
Cuts at: 2415 2688 2803 2880 3003 3306 4039 4928 6557
Size: 273 115 77 123 303 733 889 1629
Cuts at: 6557 8
Size: 43
Fragments arranged by size:
1629 1425 889 733 364 303 275 273
177 123 115 77 54 50 44 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3003 3306 4039 1537
Size: 275 1191 303 733 4090
Fragments arranged by size:
4090 1191 733 303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2945 3388 4522 1353
Size: 1592 443 1134 3423
Fragments arranged by size:
3423 1592 1134 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3287 5179 5553 5699 387
Size: 1064 138 1698 1892 374 146 1280
Fragments arranged by size:
1892 1698 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2701 2701
Size: 6592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2501 2552 2926 3260 3391 3406
Size: 393 716 153 51 374 334 131 15
Cuts at: 3406 3418 3435 3816 4655 5252 6039 6307 1239
Size: 12 17 381 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 716 597 393 381 374
334 268 153 131 51 17 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2453 2453
Size: 6592
EcoO109I rG'GnC_Cy
Cuts at: 2048 2049 2263 3109 3110 3220 3478 3479 3486
Size: 1 214 846 1 110 258 1 7
Cuts at: 3486 3523 3621 3842 2048
Size: 37 98 221 4798
Fragments arranged by size:
4798 846 258 221 214 110 98 37
7 1 1 1
EcoRI G'AATT_C
Cuts at: 3901 4279 3901
Size: 378 6214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 6592
FspI TGC'GCA
Cuts at: 2309 2609 3233 5098 2309
Size: 300 624 1865 3803
Fragments arranged by size:
3803 1865 624 300
HaeII r_GCGC'y
Cuts at: 1678 2228 2323 2335 2766 3026 3749 5739 1678
Size: 550 95 12 431 260 723 1990 2531
Fragments arranged by size:
2531 1990 723 550 431 260 95 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2567 2599 2879
Size: 32 135 32 539 32 424 32 280
Cuts at: 2879 2911 3784 3816 5233 5265 5307 5339 1373
Size: 32 873 32 1417 32 42 32 2626
Fragments arranged by size:
2626 1417 873 539 424 280 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 3936 4182 669
Size: 988 281 1024 974 246 3079
Fragments arranged by size:
3079 1024 988 974 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 4182 4182
Size: 6592
KasI G'GCGC_C
Cuts at: 2224 3022 2224
Size: 798 5794
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 4191 4191
Size: 6592
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5706 5890 1305
Size: 4401 184 2007
Fragments arranged by size:
4401 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2373 2417 2690 2805 2882
Size: 54 1425 343 541 44 273 115 77
Cuts at: 2882 10
Size: 3720
Fragments arranged by size:
3720 1425 541 343 273 115 77 54
44
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2277 2507 2888 2912 4550 4909
Size: 648 1015 96 230 381 24 1638 359
Cuts at: 4909 5068 518
Size: 159 2042
Fragments arranged by size:
2042 1638 1015 648 381 359 230 159
96 24
MspA1I CmG'CkG
Cuts at: 1606 2137 2625 3541 4008 4685 5833 6078 1606
Size: 531 488 916 467 677 1148 245 2120
Fragments arranged by size:
2120 1148 916 677 531 488 467 245
NaeI GCC'GGC
Cuts at: 3170 3170
Size: 6592
NarI GG'CG_CC
Cuts at: 2225 3023 2225
Size: 798 5794
NcoI C'CATG_G
Cuts at: 513 1065 2004 2023 2272 513
Size: 552 939 19 249 4833
Fragments arranged by size:
4833 939 552 249 19
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6592
NgoMIV G'CCGG_C
Cuts at: 3168 3168
Size: 6592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 2893 3238 3950 5495 1132
Size: 97 1664 345 712 1545 2229
Fragments arranged by size:
2229 1664 1545 712 345 97
PasI CC'CwG_GG
Cuts at: 1348 2045 2899 3271 3482 3549 1348
Size: 697 854 372 211 67 4391
Fragments arranged by size:
4391 854 697 372 211 67
PciI A'CATG_T
Cuts at: 2889 5491 2889
Size: 2602 3990
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3223 1108
Size: 2115 4477
PfoI T'CCnGG_A
Cuts at: 2115 3216 2115
Size: 1101 5491
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 3220 3523 2263
Size: 957 303 5332
Fragments arranged by size:
5332 957 303
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6592
PsiI TTA'TAA
Cuts at: 4162 4162
Size: 6592
PspOMI G'GGCC_C
Cuts at: 2048 2214 3109 3158 3328 3478 3485 3621 2048
Size: 166 895 49 170 150 7 136 5019
Fragments arranged by size:
5019 895 170 166 150 136 49 7
PstI C_TGCA'G
Cuts at: 830 3132 3285 3944 830
Size: 2302 153 659 3478
Fragments arranged by size:
3478 2302 659 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
SacI G_AGCT'C
Cuts at: 2703 2703
Size: 6592
SacII CC_GC'GG
Cuts at: 2626 2626
Size: 6592
SalI G'TCGA_C
Cuts at: 1936 2960 3934 1936
Size: 1024 974 4594
Fragments arranged by size:
4594 1024 974
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 2516 3041 4840 6552 2516
Size: 525 1799 1712 2556
Fragments arranged by size:
2556 1799 1712 525
SexAI A'CCwGG_T
Cuts at: 3222 3222
Size: 6592
SfcI C'TryA_G
Cuts at: 826 1045 3128 3281 3457 3940 5075 5756 5947
Size: 219 2083 153 176 483 1135 681 191
Cuts at: 5947 826
Size: 1471
Fragments arranged by size:
2083 1471 1135 681 483 219 191 176
153
SfiI GGCCn_nnn'nGGCC
Cuts at: 3314 3314
Size: 6592
SfoI GGC'GCC
Cuts at: 2226 3024 2226
Size: 798 5794
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3115 3163 3919 1872
Size: 1243 48 756 4545
Fragments arranged by size:
4545 1243 756 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 4696 5597 5859 820
Size: 197 926 780 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 780 262 197
SnaBI TAC'GTA
Cuts at: 493 4383 493
Size: 3890 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3238 3950 1132
Size: 2106 712 3774
Fragments arranged by size:
3774 2106 712
SspI AAT'ATT
Cuts at: 5 52 4516 5
Size: 47 4464 2081
Fragments arranged by size:
4464 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6592
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 2272 3027 3649 4031 513
Size: 552 939 19 249 755 622 382 3074
Fragments arranged by size:
3074 939 755 622 552 382 249 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2630 3756 4619 4636 4789 4974 5419
Size: 1255 1050 1126 863 17 153 185 445
Cuts at: 5419 325
Size: 1498
Fragments arranged by size:
1498 1255 1126 1050 863 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 2524 3039
Size: 274 80 33 51 1443 537 10 515
Cuts at: 3039 4838 6550 96
Size: 1799 1712 138
Fragments arranged by size:
1799 1712 1443 537 515 274 138 80
51 33 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6552 6584 6552
Size: 32 6560
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6592
XbaI T'CTAG_A
Cuts at: 3928 3928
Size: 6592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 2773
Size: 6592
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 3113 3161 3917 1870
Size: 1243 48 756 4545
Fragments arranged by size:
4545 1243 756 48
XmnI GAAnn'nnTTC
Cuts at: 4721 4721
Size: 6592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4400 276
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SacII SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstXI DraIII EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SanDI SapI SrfI SwaI