Restriction map for
pFN21AB3968
(Circular) (Six-base) MAPSORT of: pFN21AB3968.seq Check: 5829 from: 1 to: 6040
pFN21AB3968 6040 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2352 3189 3383 1937
Size: 415 837 194 4594
Fragments arranged by size:
4594 837 415 194
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4103 5487 5755 1632
Size: 2471 1384 268 1917
Fragments arranged by size:
2471 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 2589 4939 5916 1203
Size: 1386 2350 977 1327
Fragments arranged by size:
2350 1386 1327 977
AgeI A'CCGG_T
Cuts at: 2502 4871 2502
Size: 2369 3671
AhdI GACnn_n'nnGTC
Cuts at: 2062 4769 2062
Size: 2707 3333
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 2144 3166 3244 3783 5355 2144
Size: 1022 78 539 1572 2829
Fragments arranged by size:
2829 1572 1022 539 78
ApaI G_GGCC'C
Cuts at: 2179 2179
Size: 6040
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 3365 1870
Size: 73 108 1314 4545
Fragments arranged by size:
4545 1314 108 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2179 2922 3359 1391
Size: 788 743 437 4072
Fragments arranged by size:
4072 788 743 437
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 2783 928
Size: 971 285 599 4185
Fragments arranged by size:
4185 971 599 285
BbvCI CC'TCA_GC
Cuts at: 2015 2015
Size: 6040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3135 4651 136
Size: 107 122 71 631 605 1463 1516 1525
Fragments arranged by size:
1525 1516 1463 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 2387 3457 2387
Size: 1070 4970
Bme1580I G_kGCm'C
Cuts at: 1790 2179 4101 5257 1790
Size: 389 1922 1156 2573
Fragments arranged by size:
2573 1922 1156 389
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
Bpu10I CC'TnA_GC
Cuts at: 2015 2015
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3297 4703 882
Size: 617 1798 1406 2219
Fragments arranged by size:
2219 1798 1406 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 2291 6034 2291
Size: 3743 2297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2502 2994 4473 4871 5145 5292 5703 2502
Size: 492 1479 398 274 147 411 2839
Fragments arranged by size:
2839 1479 492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 1178
Size: 30 165 30 651 30 5134
Fragments arranged by size:
5134 651 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6040
BseYI C'CCAG_C
Cuts at: 5243 5739 5243
Size: 496 5544
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4101 4186 5257 4101
Size: 85 1071 4884
Fragments arranged by size:
4884 1071 85
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2922 3359 4101 4186 5257 1391
Size: 399 389 743 437 742 85 1071 2174
Fragments arranged by size:
2174 1071 743 742 437 399 389 85
BspEI T'CCGG_A
Cuts at: 2994 5703 2994
Size: 2709 3331
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3395 844
Size: 2551 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2612 4535 4709 65
Size: 1183 1364 1923 174 1396
Fragments arranged by size:
1923 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2502 4684 4871 1700
Size: 110 228 464 2182 187 2869
Fragments arranged by size:
2869 2182 464 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 3370 3737 4122 4139 5591 5677 5689 6035
Size: 1398 367 385 17 1452 86 12 346
Cuts at: 6035 1972
Size: 1977
Fragments arranged by size:
1977 1452 1398 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3190 3190
Size: 6040
BtgI C'CryG_G
Cuts at: 513 1065 3130 513
Size: 552 2065 3423
Fragments arranged by size:
3423 2065 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3007 3178 487
Size: 683 56 1781 171 3349
Fragments arranged by size:
3349 1781 683 171 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3097 3344 3097
Size: 247 5793
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 3127 3171
Size: 54 1425 50 275 18 405 892 44
Cuts at: 3171 3254 3487 4376 6005 8
Size: 83 233 889 1629 43
Fragments arranged by size:
1629 1425 892 889 405 275 233 83
54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 3970 1353
Size: 1113 1504 3423
Fragments arranged by size:
3423 1504 1113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4103 4700 5487 5755 1239
Size: 393 2471 597 787 268 1524
Fragments arranged by size:
2471 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2377 2377
Size: 6040
EcoO109I rG'GnC_Cy
Cuts at: 2164 2175 2176 3212 2164
Size: 11 1 1036 4992
Fragments arranged by size:
4992 1036 11 1
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2054 2080 2084 2086
Size: 32 135 32 476 6 26 4 2
Cuts at: 2086 2116 2850 2882 4681 4713 4755 4787 1373
Size: 30 734 32 1799 32 42 32 2626
Fragments arranged by size:
2626 1799 734 476 135 42 32 32
32 32 32 30 26 6 4 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2726 5154 5338 1305
Size: 903 518 2428 184 2007
Fragments arranged by size:
2428 2007 903 518 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 3129 3173 10
Size: 54 1425 343 405 892 44 2877
Fragments arranged by size:
2877 1425 892 405 343 54 44
MslI CAynn'nnrTG
Cuts at: 518 1166 2361 3998 4357 4516 518
Size: 648 1195 1637 359 159 2042
Fragments arranged by size:
2042 1637 1195 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2391 3456 4133 5281 5526 1606
Size: 785 1065 677 1148 245 2120
Fragments arranged by size:
2120 1148 1065 785 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3130 513
Size: 552 2065 3423
Fragments arranged by size:
3423 2065 552
NdeI CA'TA_TG
Cuts at: 387 2362 387
Size: 1975 4065
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2545 2593 3398 4943 1132
Size: 97 1316 48 805 1545 2229
Fragments arranged by size:
2229 1545 1316 805 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 2589 4939 2589
Size: 2350 3690
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 6040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PspOMI G'GGCC_C
Cuts at: 2175 2175
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2149 2583 2943 3392 830
Size: 1319 434 360 449 3478
Fragments arranged by size:
3478 1319 449 434 360
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2391 2391
Size: 6040
RsrII CG'GwC_CG
Cuts at: 2992 2992
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SapI GCTCTTCn'nnn_
Cuts at: 2466 2466
Size: 6040
SbfI CC_TGCA'GG
Cuts at: 2943 3392 2943
Size: 449 5591
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2145 2579 2939 3388 4523 5204 5395
Size: 219 1100 434 360 449 1135 681 191
Cuts at: 5395 826
Size: 1471
Fragments arranged by size:
1471 1135 1100 681 449 434 360 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 3130 3479 513
Size: 552 2065 349 3074
Fragments arranged by size:
3074 2065 552 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2203 4067 4084 4237 4422 4867 325
Size: 1255 623 1864 17 153 185 445 1498
Fragments arranged by size:
1864 1498 1255 623 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2548 2880 2946
Size: 274 80 33 51 1443 571 332 66
Cuts at: 2946 4286 5998 96
Size: 1340 1712 138
Fragments arranged by size:
1712 1443 1340 571 332 274 138 80
66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 2975
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI