Restriction map for
pFN21AB3964
(Circular) (Six-base) MAPSORT of: pFN21AB3964.seq Check: 3226 from: 1 to: 5272
pFN21AB3964 5272 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2027 2027
Size: 5272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3082 278
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2615 1937
Size: 678 4594
Acc65I G'GTAC_C
Cuts at: 2593 2593
Size: 5272
AclI AA'CG_TT
Cuts at: 1563 3399 3772 1563
Size: 1836 373 3063
Fragments arranged by size:
3063 1836 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3335 4719 4987 1632
Size: 1703 1384 268 1917
Fragments arranged by size:
1917 1703 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5075
AflIII A'CryG_T
Cuts at: 1203 4171 5148 1203
Size: 2968 977 1327
Fragments arranged by size:
2968 1327 977
AgeI A'CCGG_T
Cuts at: 4103 4103
Size: 5272
AhdI GACnn_n'nnGTC
Cuts at: 4001 4001
Size: 5272
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5272
AlwNI CAG_nnn'CTG
Cuts at: 3015 4587 3015
Size: 1572 3700
ApaLI G'TGCA_C
Cuts at: 3329 4485 3329
Size: 1156 4116
ApoI r'AATT_y
Cuts at: 1493 2581 2807 2959 5068 1493
Size: 1088 226 152 2109 1697
Fragments arranged by size:
2109 1697 1088 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3826 160
Size: 872 2794 1606
Fragments arranged by size:
2794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 1870
Size: 73 654 4545
Fragments arranged by size:
4545 654 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2483 2516 2483
Size: 33 5239
BamHI G'GATC_C
Cuts at: 2602 2969 2602
Size: 367 4905
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2593 4048 618
Size: 325 217 627 806 1455 1842
Fragments arranged by size:
1842 1455 806 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2591 1391
Size: 1200 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4301
BbvCI CC'TCA_GC
Cuts at: 2437 2437
Size: 5272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3463 3497 1903
Size: 34 1526 34 3678
Fragments arranged by size:
3678 1526 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2167 3164 4374 2167
Size: 997 1210 3065
Fragments arranged by size:
3065 1210 997
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3883 136
Size: 107 122 71 631 605 2211 1525
Fragments arranged by size:
2211 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5267 5267
Size: 5272
BlpI GC'TnA_GC
Cuts at: 2689 2689
Size: 5272
Bme1580I G_kGCm'C
Cuts at: 1790 3333 4489 1790
Size: 1543 1156 2573
Fragments arranged by size:
2573 1543 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5272
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3003 3961 442
Size: 416 53 83 399 24 1586 958 1753
Fragments arranged by size:
1753 1586 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3932 1239
Size: 2693 2579
Bpu10I CC'TnA_GC
Cuts at: 2437 2437
Size: 5272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4262 4560 3397
Size: 865 298 4109
Fragments arranged by size:
4109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 3935 882
Size: 617 703 1733 2219
Fragments arranged by size:
2219 1733 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 3063 493
Size: 711 1859 2702
Fragments arranged by size:
2702 1859 711
BsaBI GATnn'nnATC
Cuts at: 5266 5266
Size: 5272
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3079 3461 4096 275
Size: 53 83 186 936 1546 382 635 1451
Fragments arranged by size:
1546 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3705 4103 4377 4524 4935 3705
Size: 398 274 147 411 4042
Fragments arranged by size:
4042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5047
Fragments arranged by size:
5047 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5272
BseYI C'CCAG_C
Cuts at: 4475 4971 4475
Size: 496 4776
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4699
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2722 3483 4511 1540
Size: 275 186 721 761 1028 2301
Fragments arranged by size:
2301 1028 761 721 275 186
BsiHKAI G_wGCw'C
Cuts at: 3333 3418 4489 3333
Size: 85 1071 4116
Fragments arranged by size:
4116 1071 85
BsmI GAATG_Cn'
Cuts at: 2783 2876 2783
Size: 93 5179
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2591 3333 3418 4489 1391
Size: 399 801 742 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 399 85
BspEI T'CCGG_A
Cuts at: 4935 4935
Size: 5272
BspHI T'CATG_A
Cuts at: 3161 4891 3161
Size: 1730 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2027 2627 844
Size: 1183 600 3489
Fragments arranged by size:
3489 1183 600
BsrBI CCG'CTC
Cuts at: 1394 1823 3159 1394
Size: 429 1336 3507
Fragments arranged by size:
3507 1336 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3767 3941 65
Size: 1183 2519 174 1396
Fragments arranged by size:
2519 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3916 4103 1700
Size: 110 2106 187 2869
Fragments arranged by size:
2869 2106 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5272
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5272
BssSI C'ACGA_G
Cuts at: 3332 4344 3332
Size: 1012 4260
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5272
BstBI TT'CG_AA
Cuts at: 4076 4076
Size: 5272
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5272
BstYI r'GATC_y
Cuts at: 1972 2602 2969 3354 3371 4823 4909 4921 5267
Size: 630 367 385 17 1452 86 12 346
Cuts at: 5267 1972
Size: 1977
Fragments arranged by size:
1977 1452 630 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4720
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4533
Fragments arranged by size:
4533 683 56
BtsI GCAGTG_nn'
Cuts at: 2530 2788 3582 3602 2530
Size: 258 794 20 4200
Fragments arranged by size:
4200 794 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5237
DraI TTT'AAA
Cuts at: 2576 2576
Size: 5272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4279 809
Size: 507 2963 1802
Fragments arranged by size:
2963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 2719 3608
Size: 54 1425 50 275 18 573 316 889
Cuts at: 3608 5237 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 573 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2719 1537
Size: 275 907 4090
Fragments arranged by size:
4090 907 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 3202 1353
Size: 831 1018 3423
Fragments arranged by size:
3423 1018 831
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3859 4233 4379 387
Size: 1064 138 2270 374 146 1280
Fragments arranged by size:
2270 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3335 3932 4719 4987 1239
Size: 393 1703 597 787 268 1524
Fragments arranged by size:
1703 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2581 2959 2581
Size: 378 4894
FspI TGC'GCA
Cuts at: 3778 3778
Size: 5272
HaeII r_GCGC'y
Cuts at: 1678 4419 1678
Size: 2741 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3913 3945 3987 4019 1373
Size: 32 135 32 2341 32 42 32 2626
Fragments arranged by size:
2626 2341 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2616 2862 669
Size: 988 281 678 246 3079
Fragments arranged by size:
3079 988 678 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5272
HpaI GTT'AAC
Cuts at: 2862 2862
Size: 5272
KpnI G_GTAC'C
Cuts at: 2597 2597
Size: 5272
MfeI C'AATT_G
Cuts at: 2871 2871
Size: 5272
MluI A'CGCG_T
Cuts at: 5148 5148
Size: 5272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2011 2142 4386 4570 1305
Size: 706 131 2244 184 2007
Fragments arranged by size:
2244 2007 706 184 131
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 10
Size: 54 1425 343 573 2877
Fragments arranged by size:
2877 1425 573 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 3589 3748 518
Size: 648 2064 359 159 2042
Fragments arranged by size:
2064 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2688 3365 4513 4758 1606
Size: 574 508 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 574 508 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4720
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5272
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5272
NotI GC'GGCC_GC
Cuts at: 2719 2719
Size: 5272
NspI r_CATG'y
Cuts at: 1132 1229 2630 4175 1132
Size: 97 1401 1545 2229
Fragments arranged by size:
2229 1545 1401 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5272
PciI A'CATG_T
Cuts at: 4171 4171
Size: 5272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 1108
Size: 1293 3979
PmeI GTTT'AAAC
Cuts at: 2576 2576
Size: 5272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 4854 4886 3341
Size: 32 1481 32 3727
Fragments arranged by size:
3727 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5272
PsiI TTA'TAA
Cuts at: 2842 2842
Size: 5272
PstI C_TGCA'G
Cuts at: 830 2624 830
Size: 1794 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5272
PvuII CAG'CTG
Cuts at: 2180 2180
Size: 5272
SalI G'TCGA_C
Cuts at: 1936 2614 1936
Size: 678 4594
SbfI CC_TGCA'GG
Cuts at: 2624 2624
Size: 5272
ScaI AGT'ACT
Cuts at: 3520 5232 3520
Size: 1712 3560
SfcI C'TryA_G
Cuts at: 826 1045 2290 2620 3755 4436 4627 826
Size: 219 1245 330 1135 681 191 1471
Fragments arranged by size:
1471 1245 1135 681 330 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5272
SmaI CCC'GGG
Cuts at: 1872 2599 1872
Size: 727 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4277 4539 820
Size: 197 926 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3063 493
Size: 2570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5272
SphI G_CATG'C
Cuts at: 1132 2630 1132
Size: 1498 3774
SspI AAT'ATT
Cuts at: 5 52 3196 5
Size: 47 3144 2081
Fragments arranged by size:
3144 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5272
StyI C'CwwG_G
Cuts at: 513 1065 2711 513
Size: 552 1646 3074
Fragments arranged by size:
3074 1646 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3299 3316 3469 3654 4099 325
Size: 1255 1719 17 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2013 2193 3518
Size: 274 80 33 51 1443 36 180 1325
Cuts at: 3518 5230 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1325 274 180 138 80 51
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2049 2081 5232 5264 2049
Size: 32 3151 32 2057
Fragments arranged by size:
3151 2057 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5272
XbaI T'CTAG_A
Cuts at: 2608 2608
Size: 5272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5272
XmaI C'CCGG_G
Cuts at: 1870 2597 1870
Size: 727 4545
XmnI GAAnn'nnTTC
Cuts at: 3401 3401
Size: 5272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3080 276
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI