Restriction map for
pFN21AB3956
(Circular) (Six-base) MAPSORT of: pFN21AB3956.seq Check: 4414 from: 1 to: 5857
pFN21AB3956 5857 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2071 3173 3920 5304 5572 1632
Size: 439 1102 747 1384 268 1917
Fragments arranged by size:
1917 1384 1102 747 439 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2819 1184
Size: 1635 4222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 5825
AlwNI CAG_nnn'CTG
Cuts at: 2771 3600 5172 2771
Size: 829 1572 3456
Fragments arranged by size:
3456 1572 829
ApaI G_GGCC'C
Cuts at: 2708 2708
Size: 5857
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 3182 1870
Size: 73 802 437 4545
Fragments arranged by size:
4545 802 437 73
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 3118 3178 4633 618
Size: 325 217 627 1082 249 60 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 249 217 60
BanII G_rGCy'C
Cuts at: 1391 2708 2754 3176 1391
Size: 1317 46 422 4072
Fragments arranged by size:
4072 1317 422 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2825 928
Size: 971 926 3960
Fragments arranged by size:
3960 971 926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 2985 1548
Size: 1437 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2444 4468
Size: 107 122 71 631 605 616 156 2024
Cuts at: 4468 136
Size: 1525
Fragments arranged by size:
2024 1525 631 616 605 156 122 107
71
BglII A'GATC_T
Cuts at: 2619 2634 5852 2619
Size: 15 3218 2624
Fragments arranged by size:
3218 2624 15
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 2708 2872 3123 3918 5074 1790
Size: 918 164 251 795 1156 2573
Fragments arranged by size:
2573 1156 918 795 251 164
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4517 1239
Size: 3278 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 2380 2953 3648 493
Size: 711 1143 33 573 695 2702
Fragments arranged by size:
2702 1143 711 695 573 33
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3061 3664 4046 4681
Size: 53 83 186 936 1528 603 382 635
Cuts at: 4681 275
Size: 1451
Fragments arranged by size:
1528 1451 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 2741 2771 1178
Size: 30 165 30 648 30 660 30 4264
Fragments arranged by size:
4264 660 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 1186
Size: 873 4984
BseYI C'CCAG_C
Cuts at: 2865 3069 5060 5556 2865
Size: 204 1991 496 3166
Fragments arranged by size:
3166 1991 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3038 1163
Size: 573 1302 3982
Fragments arranged by size:
3982 1302 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2754 2835 3918 4003 5074 2385
Size: 369 81 1083 85 1071 3168
Fragments arranged by size:
3168 1083 1071 369 85 81
BsmI GAATG_Cn'
Cuts at: 2841 3368 3461 2841
Size: 527 93 5237
Fragments arranged by size:
5237 527 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2419 2431 2419
Size: 12 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2708 2754 2835 2872 3123 3176
Size: 399 595 323 46 81 37 251 53
Cuts at: 3176 3918 4003 5074 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 323 251 85
81 53 46 37
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2412 3212 844
Size: 1568 800 3489
Fragments arranged by size:
3489 1568 800
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 2277 2448 2553 4352 4526 65
Size: 1183 927 102 171 105 1799 174 1396
Fragments arranged by size:
1799 1396 1183 927 174 171 105 102
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 2713 1184
Size: 1529 4328
BstYI r'GATC_y
Cuts at: 1972 2619 2634 2739 3187 3554 3939 3956 5408
Size: 647 15 105 448 367 385 17 1452
Cuts at: 5408 5494 5506 5852 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 647 448 385 367 346 105
86 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2492 513
Size: 552 939 488 3878
Fragments arranged by size:
3878 939 552 488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2950 487
Size: 683 56 1724 3394
Fragments arranged by size:
3394 1724 683 56
BtsI GCAGTG_nn'
Cuts at: 2940 3373 4167 4187 2940
Size: 433 794 20 4610
Fragments arranged by size:
4610 794 433 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DraIII CAC_nnn'GTG
Cuts at: 2997 2997
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2310 2374
Size: 54 1425 50 275 18 459 21 64
Cuts at: 2374 2445 2629 3304 4193 5822 8
Size: 71 184 675 889 1629 43
Fragments arranged by size:
1629 1425 889 675 459 275 184 71
64 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 3126 3787 1353
Size: 1331 442 661 3423
Fragments arranged by size:
3423 1331 661 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2752 2752
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 3173 3920 4517 5304 5572 1239
Size: 393 439 1102 747 597 787 268 1524
Fragments arranged by size:
1524 1102 787 747 597 439 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2705 2705
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
EcoRV GAT'ATC
Cuts at: 2465 2465
Size: 5857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 5857
FspI TGC'GCA
Cuts at: 2258 2408 4363 2258
Size: 150 1955 3752
Fragments arranged by size:
3752 1955 150
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2488 2520 4498
Size: 32 135 32 479 32 405 32 1978
Cuts at: 4498 4530 4572 4604 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 479 405 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2398 3201 3447 669
Size: 988 281 460 803 246 3079
Fragments arranged by size:
3079 988 803 460 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 4971 5155 1305
Size: 1017 2649 184 2007
Fragments arranged by size:
2649 2007 1017 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 2312 2376 2447 2631
Size: 54 1425 343 459 21 64 71 184
Cuts at: 2631 10
Size: 3236
Fragments arranged by size:
3236 1425 459 343 184 71 64 54
21
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3815 4174 4333 518
Size: 648 1803 846 359 159 2042
Fragments arranged by size:
2042 1803 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3073 3273 3950 5098 5343 1606
Size: 1467 200 677 1148 245 2120
Fragments arranged by size:
2120 1467 1148 677 245 200
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3215 4760 1132
Size: 97 1986 1545 2229
Fragments arranged by size:
2229 1986 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2771 1348
Size: 1423 4434
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PmlI CAC'GTG
Cuts at: 2380 2380
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 3926 3958 5439 5471 2741
Size: 32 1153 32 1481 32 3127
Fragments arranged by size:
3127 1481 1153 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2704 2704
Size: 5857
PstI C_TGCA'G
Cuts at: 830 3209 830
Size: 2379 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
SacI G_AGCT'C
Cuts at: 2754 2754
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 3032 4105 5817 3032
Size: 1073 1712 3072
Fragments arranged by size:
3072 1712 1073
SfcI C'TryA_G
Cuts at: 826 1045 3205 4340 5021 5212 826
Size: 219 2160 1135 681 191 1471
Fragments arranged by size:
2160 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2747 3184 1872
Size: 875 437 4545
Fragments arranged by size:
4545 875 437
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2004 2529 3296 513
Size: 552 939 525 767 3074
Fragments arranged by size:
3074 939 767 552 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2053 3884 3901 4054 4239 4684 325
Size: 1255 473 1831 17 153 185 445 1498
Fragments arranged by size:
1831 1498 1255 473 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3030 4103 5815
Size: 274 80 33 51 1443 1053 1073 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1443 1073 1053 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2440 3133 2440
Size: 693 5164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2745 3182 1870
Size: 875 437 4545
Fragments arranged by size:
4545 875 437
XmnI GAAnn'nnTTC
Cuts at: 2621 3032 3986 2621
Size: 411 954 4492
Fragments arranged by size:
4492 954 411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI