Restriction map for
pFN21AB3947
(Circular) (Six-base) MAPSORT of: pFN21AB3947.seq Check: 6152 from: 1 to: 7369
pFN21AB3947 7369 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5179 278
Size: 53 83 186 936 3643 2468
Fragments arranged by size:
3643 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3112 3349 3895 4712 1937
Size: 1175 237 546 817 4594
Fragments arranged by size:
4594 1175 817 546 237
Acc65I G'GTAC_C
Cuts at: 2549 4690 2549
Size: 2141 5228
AclI AA'CG_TT
Cuts at: 1563 5496 5869 1563
Size: 3933 373 3063
Fragments arranged by size:
3933 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3861 5432 6816 7084 1632
Size: 2229 1571 1384 268 1917
Fragments arranged by size:
2229 1917 1571 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7172
AflIII A'CryG_T
Cuts at: 1203 2109 6268 7245 1203
Size: 906 4159 977 1327
Fragments arranged by size:
4159 1327 977 906
AgeI A'CCGG_T
Cuts at: 6200 6200
Size: 7369
AhdI GACnn_n'nnGTC
Cuts at: 4589 6098 4589
Size: 1509 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7369
AlwNI CAG_nnn'CTG
Cuts at: 2820 5112 6684 2820
Size: 2292 1572 3505
Fragments arranged by size:
3505 2292 1572
ApaLI G'TGCA_C
Cuts at: 5426 6582 5426
Size: 1156 6213
ApoI r'AATT_y
Cuts at: 1493 2248 3383 3653 4210 4678 4904 5056 7165
Size: 755 1135 270 557 468 226 152 2109
Cuts at: 7165 1493
Size: 1697
Fragments arranged by size:
2109 1697 1135 755 557 468 270 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5923 160
Size: 872 4891 1606
Fragments arranged by size:
4891 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 2721 4694 1870
Size: 73 730 48 1973 4545
Fragments arranged by size:
4545 1973 730 73 48
BamHI G'GATC_C
Cuts at: 4699 5066 4699
Size: 367 7002
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 3737 4690 6145 618
Size: 325 217 627 762 1188 953 1455 1842
Fragments arranged by size:
1842 1455 1188 953 762 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4223 4688 1391
Size: 2832 465 4072
Fragments arranged by size:
4072 2832 465
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3782 4667 928
Size: 971 1883 885 3630
Fragments arranged by size:
3630 1883 971 885
BbvCI CC'TCA_GC
Cuts at: 4443 4443
Size: 7369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5560 5594 1903
Size: 34 3623 34 3678
Fragments arranged by size:
3678 3623 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5261 6471 5261
Size: 1210 6159
BclI T'GATC_A
Cuts at: 1548 2949 1548
Size: 1401 5968
BfrBI ATG'CAT
Cuts at: 2029 2214 2029
Size: 185 7184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5980 136
Size: 107 122 71 631 605 4308 1525
Fragments arranged by size:
4308 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2130 7364 2130
Size: 5234 2135
BlpI GC'TnA_GC
Cuts at: 2708 3423 4786 2708
Size: 715 1363 5291
Fragments arranged by size:
5291 1363 715
Bme1580I G_kGCm'C
Cuts at: 1790 5430 6586 1790
Size: 3640 1156 2573
Fragments arranged by size:
3640 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3116 3893 1318
Size: 1798 777 4794
Fragments arranged by size:
4794 1798 777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3773 5100 6058
Size: 416 53 83 399 24 2356 1327 958
Cuts at: 6058 442
Size: 1753
Fragments arranged by size:
2356 1753 1327 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3515 6029 1239
Size: 2276 2514 2579
Fragments arranged by size:
2579 2514 2276
Bpu10I CC'TnA_GC
Cuts at: 4443 4443
Size: 7369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2293 5494 6359 6657 2293
Size: 3201 865 298 3005
Fragments arranged by size:
3201 3005 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6032 882
Size: 617 4533 2219
Fragments arranged by size:
4533 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3797 5160 493
Size: 711 2593 1363 2702
Fragments arranged by size:
2702 2593 1363 711
BsaBI GATnn'nnATC
Cuts at: 7363 7363
Size: 7369
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5176 5558 6193 275
Size: 53 83 186 936 3643 382 635 1451
Fragments arranged by size:
3643 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5802 6200 6474 6621 7032 5802
Size: 398 274 147 411 6139
Fragments arranged by size:
6139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4623 4653 1178
Size: 30 165 30 3220 30 3894
Fragments arranged by size:
3894 3220 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2829 3253 1186
Size: 1643 424 5302
Fragments arranged by size:
5302 1643 424
BseYI C'CCAG_C
Cuts at: 2641 3433 4232 4547 6572 7068 2641
Size: 792 799 315 2025 496 2942
Fragments arranged by size:
2942 2025 799 792 496 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4372 1163
Size: 573 2636 4160
Fragments arranged by size:
4160 2636 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4819 5580 6608 1540
Size: 275 186 2818 761 1028 2301
Fragments arranged by size:
2818 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5430 5515 6586 5430
Size: 85 1071 6213
Fragments arranged by size:
6213 1071 85
BsmI GAATG_Cn'
Cuts at: 2031 3370 4880 4973 2031
Size: 1339 1510 93 4427
Fragments arranged by size:
4427 1510 1339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4223 4688 5430 5515 6586 1391
Size: 399 2433 465 742 85 1071 2174
Fragments arranged by size:
2433 2174 1071 742 465 399 85
BspEI T'CCGG_A
Cuts at: 7032 7032
Size: 7369
BspHI T'CATG_A
Cuts at: 2628 3466 5258 6988 2628
Size: 838 1792 1730 3009
Fragments arranged by size:
3009 1792 1730 838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4090 4724 844
Size: 3246 634 3489
Fragments arranged by size:
3489 3246 634
BsrBI CCG'CTC
Cuts at: 1394 1823 5256 1394
Size: 429 3433 3507
Fragments arranged by size:
3507 3433 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4287 5864 6038 65
Size: 1183 3039 1577 174 1396
Fragments arranged by size:
3039 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6013 6200 1700
Size: 110 4203 187 2869
Fragments arranged by size:
4203 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7369
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7369
BssSI C'ACGA_G
Cuts at: 2478 3771 4019 5429 6441 2478
Size: 1293 248 1410 1012 3406
Fragments arranged by size:
3406 1410 1293 1012 248
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2432 794
Size: 1638 5731
BstBI TT'CG_AA
Cuts at: 2023 6173 2023
Size: 4150 3219
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7369
BstYI r'GATC_y
Cuts at: 1972 2130 4699 5066 5451 5468 6920 7006 7018
Size: 158 2569 367 385 17 1452 86 12
Cuts at: 7018 7364 1972
Size: 346 1977
Fragments arranged by size:
2569 1977 1452 385 367 346 158 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3966 4227 513
Size: 552 2901 261 3655
Fragments arranged by size:
3655 2901 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6630
Fragments arranged by size:
6630 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 3858 4885 5679 5699 2188
Size: 1670 1027 794 20 3858
Fragments arranged by size:
3858 1670 1027 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7369
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7334
DraI TTT'AAA
Cuts at: 4673 4673
Size: 7369
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6376 809
Size: 507 5060 1802
Fragments arranged by size:
5060 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3693 4816 5705
Size: 54 1425 50 275 18 1863 1123 889
Cuts at: 5705 7334 8
Size: 1629 43
Fragments arranged by size:
1863 1629 1425 1123 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4816 1537
Size: 275 3004 4090
Fragments arranged by size:
4090 3004 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 3159 4102 5299 1353
Size: 807 999 943 1197 3423
Fragments arranged by size:
3423 1197 999 943 807
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5956 6330 6476 387
Size: 1064 138 4367 374 146 1280
Fragments arranged by size:
4367 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3515 3861 5432 6029 6816 7084 1239
Size: 393 1883 346 1571 597 787 268 1524
Fragments arranged by size:
1883 1571 1524 787 597 393 346 268
EcoO109I rG'GnC_Cy
Cuts at: 2498 3629 2498
Size: 1131 6238
EcoRI G'AATT_C
Cuts at: 4678 5056 4678
Size: 378 6991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3704 3031
Size: 673 6696
FspI TGC'GCA
Cuts at: 5875 5875
Size: 7369
HaeII r_GCGC'y
Cuts at: 1678 6516 1678
Size: 4838 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2802 2815 2847 3023
Size: 32 135 32 1198 32 13 32 176
Cuts at: 3023 3055 3266 3298 3448 3480 4575 4607 6010
Size: 32 211 32 150 32 1095 32 1403
Cuts at: 6010 6042 6084 6116 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1403 1198 1095 211 176 150 135
42 32 32 32 32 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2258 4198 4713 4959 669
Size: 988 281 320 1940 515 246 3079
Fragments arranged by size:
3079 1940 988 515 320 281 246
HindIII A'AGCT_T
Cuts at: 748 2373 3509 748
Size: 1625 1136 4608
Fragments arranged by size:
4608 1625 1136
HpaI GTT'AAC
Cuts at: 4959 4959
Size: 7369
KpnI G_GTAC'C
Cuts at: 2553 4694 2553
Size: 2141 5228
MfeI C'AATT_G
Cuts at: 3103 4968 3103
Size: 1865 5504
MluI A'CGCG_T
Cuts at: 7245 7245
Size: 7369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2120 2469 3426 3598 6483 6667 1305
Size: 815 349 957 172 2885 184 2007
Fragments arranged by size:
2885 2007 957 815 349 184 172
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3695 10
Size: 54 1425 343 1863 3684
Fragments arranged by size:
3684 1863 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2108 3471 3971 5327 5686 5845
Size: 648 927 15 1363 500 1356 359 159
Cuts at: 5845 518
Size: 2042
Fragments arranged by size:
2042 1363 1356 927 648 500 359 159
15
MspA1I CmG'CkG
Cuts at: 1606 4386 4785 5462 6610 6855 1606
Size: 2780 399 677 1148 245 2120
Fragments arranged by size:
2780 2120 1148 677 399 245
NcoI C'CATG_G
Cuts at: 513 1065 3966 4227 513
Size: 552 2901 261 3655
Fragments arranged by size:
3655 2901 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7369
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7369
NotI GC'GGCC_GC
Cuts at: 4816 4816
Size: 7369
NsiI A_TGCA'T
Cuts at: 2031 2216 2031
Size: 185 7184
NspI r_CATG'y
Cuts at: 1132 1229 2113 2602 4485 4727 6272 1132
Size: 97 884 489 1883 242 1545 2229
Fragments arranged by size:
2229 1883 1545 884 489 242 97
PasI CC'CwG_GG
Cuts at: 1348 2055 3321 1348
Size: 707 1266 5396
Fragments arranged by size:
5396 1266 707
PciI A'CATG_T
Cuts at: 2109 6268 2109
Size: 4159 3210
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7369
PmeI GTTT'AAAC
Cuts at: 4673 4673
Size: 7369
PmlI CAC'GTG
Cuts at: 3797 3797
Size: 7369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5438 5470 6951 6983 5438
Size: 32 1481 32 5824
Fragments arranged by size:
5824 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2498 3629 2498
Size: 1131 6238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7369
PsiI TTA'TAA
Cuts at: 2192 3203 3555 4939 2192
Size: 1011 352 1384 4622
Fragments arranged by size:
4622 1384 1011 352
PstI C_TGCA'G
Cuts at: 830 3716 3814 4721 830
Size: 2886 98 907 3478
Fragments arranged by size:
3478 2886 907 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7369
PvuII CAG'CTG
Cuts at: 4386 4386
Size: 7369
SalI G'TCGA_C
Cuts at: 1936 4711 1936
Size: 2775 4594
SbfI CC_TGCA'GG
Cuts at: 4721 4721
Size: 7369
ScaI AGT'ACT
Cuts at: 3288 5617 7329 3288
Size: 2329 1712 3328
Fragments arranged by size:
3328 2329 1712
SfcI C'TryA_G
Cuts at: 826 1045 3075 3350 3712 3810 3896 4401 4717
Size: 219 2030 275 362 98 86 505 316
Cuts at: 4717 5852 6533 6724 826
Size: 1135 681 191 1471
Fragments arranged by size:
2030 1471 1135 681 505 362 316 275
219 191 98 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7369
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7369
SmaI CCC'GGG
Cuts at: 1872 4696 1872
Size: 2824 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2308 5473 6374 6636 820
Size: 197 926 365 3165 901 262 1553
Fragments arranged by size:
3165 1553 926 901 365 262 197
SnaBI TAC'GTA
Cuts at: 493 5160 493
Size: 4667 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7369
SphI G_CATG'C
Cuts at: 1132 4485 4727 1132
Size: 3353 242 3774
Fragments arranged by size:
3774 3353 242
SspI AAT'ATT
Cuts at: 5 52 2473 5293 5
Size: 47 2421 2820 2081
Fragments arranged by size:
2820 2421 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7369
StyI C'CwwG_G
Cuts at: 513 1065 2655 3564 3966 4146 4227 4642 4808
Size: 552 1590 909 402 180 81 415 166
Cuts at: 4808 513
Size: 3074
Fragments arranged by size:
3074 1590 909 552 415 402 180 166
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4409 5396 5413 5566 5751 6196 325
Size: 1255 2829 987 17 153 185 445 1498
Fragments arranged by size:
2829 1498 1255 987 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2324 2849 3009
Size: 274 80 33 51 1443 347 525 160
Cuts at: 3009 3286 4067 5615 7327 96
Size: 277 781 1548 1712 138
Fragments arranged by size:
1712 1548 1443 781 525 347 277 274
160 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3757 3789 7329 7361 3757
Size: 32 3540 32 3765
Fragments arranged by size:
3765 3540 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7369
XbaI T'CTAG_A
Cuts at: 4705 4705
Size: 7369
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7369
XmaI C'CCGG_G
Cuts at: 1870 4694 1870
Size: 2824 4545
XmnI GAAnn'nnTTC
Cuts at: 5498 5498
Size: 7369
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5177 276
Size: 53 83 186 936 3643 2468
Fragments arranged by size:
3643 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI