Restriction map for
pFN21AB3946
(Circular) (Six-base) MAPSORT of: pFN21AB3946.seq Check: 9855 from: 1 to: 6232
pFN21AB3946 6232 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2850 4042 278
Size: 53 83 186 936 1314 1192 2468
Fragments arranged by size:
2468 1314 1192 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3510 3575 1937
Size: 1573 65 4594
Fragments arranged by size:
4594 1573 65
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 2780 4295 5679 5947 1632
Size: 572 576 1515 1384 268 1917
Fragments arranged by size:
1917 1515 1384 576 572 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 2157 3003 5131 6108 1203
Size: 954 846 2128 977 1327
Fragments arranged by size:
2128 1327 977 954 846
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 2192 2224 3335 3367 2144
Size: 32 16 32 1111 32 5009
Fragments arranged by size:
5009 1111 32 32 32 16
AlwNI CAG_nnn'CTG
Cuts at: 2896 3975 5547 2896
Size: 1079 1572 3581
Fragments arranged by size:
3581 1572 1079
ApaI G_GGCC'C
Cuts at: 2705 2705
Size: 6232
ApaLI G'TGCA_C
Cuts at: 2666 4289 5445 2666
Size: 1623 1156 3453
Fragments arranged by size:
3453 1623 1156
ApoI r'AATT_y
Cuts at: 1493 3541 3767 3919 6028 1493
Size: 2048 226 152 2109 1697
Fragments arranged by size:
2109 2048 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2686 2914 3557 1870
Size: 73 743 228 643 4545
Fragments arranged by size:
4545 743 643 228 73
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 2269 2772 2920 3025
Size: 325 217 627 276 206 503 148 105
Cuts at: 3025 3076 3097 3118 3380 3553 5008 618
Size: 51 21 21 262 173 1455 1842
Fragments arranged by size:
1842 1455 627 503 325 276 262 217
206 173 148 105 51 21 21
BanII G_rGCy'C
Cuts at: 1391 2102 2120 2178 2409 2705 3215 3551 1391
Size: 711 18 58 231 296 510 336 4072
Fragments arranged by size:
4072 711 510 336 296 231 58 18
BbeI G_GCGC'C
Cuts at: 2067 2776 3029 3080 3101 2067
Size: 709 253 51 21 5198
Fragments arranged by size:
5198 709 253 51 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2353 2387 4423 4457 1903
Size: 34 416 34 2036 34 3678
Fragments arranged by size:
3678 2036 416 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 2115 2987
Size: 107 122 71 631 605 389 54 872
Cuts at: 2987 3065 3074 4843 136
Size: 78 9 1769 1525
Fragments arranged by size:
1769 1525 872 631 605 389 122 107
78 71 54 9
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 2408 2837 3158 3209 3420 3649 2408
Size: 429 321 51 211 229 4991
Fragments arranged by size:
4991 429 321 229 211 51
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2670 2705 3383 4293 5449 1790
Size: 484 396 35 678 910 1156 2573
Fragments arranged by size:
2573 1156 910 678 484 396 35
BmgBI CAC'GTC
Cuts at: 1318 2317 2737 2821 1318
Size: 999 420 84 4729
Fragments arranged by size:
4729 999 420 84
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3505 3963 4921
Size: 416 53 83 399 24 2088 458 958
Cuts at: 4921 442
Size: 1753
Fragments arranged by size:
2088 1753 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2281 2452 3331 4892 1239
Size: 1042 171 879 1561 2579
Fragments arranged by size:
2579 1561 1042 879 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2858 4895 882
Size: 617 1359 2037 2219
Fragments arranged by size:
2219 2037 1359 617
BsaAI yAC'GTr
Cuts at: 493 1204 3004 4023 493
Size: 711 1800 1019 2702
Fragments arranged by size:
2702 1800 1019 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2773 2847 3026
Size: 53 83 186 936 531 709 74 179
Cuts at: 3026 3077 3098 4039 4421 5056 275
Size: 51 21 941 382 635 1451
Fragments arranged by size:
1451 941 936 709 635 531 382 186
179 83 74 53 51 21
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2192 2222 2633
Size: 30 165 30 741 30 18 30 411
Cuts at: 2633 2663 2837 2867 3278 3308 3329 3335 3359
Size: 30 174 30 411 30 21 6 24
Cuts at: 3359 3365 1178
Size: 6 4045
Fragments arranged by size:
4045 741 411 411 174 165 30 30
30 30 30 30 30 24 21 18
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2486 2588 2635 2638 1186
Size: 1027 273 102 47 3 4780
Fragments arranged by size:
4780 1027 273 102 47 3
BseYI C'CCAG_C
Cuts at: 2069 2419 3180 3215 3489 5435 5931 2069
Size: 350 761 35 274 1946 496 2370
Fragments arranged by size:
2370 1946 761 496 350 274 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 2759 1163
Size: 573 777 246 4636
Fragments arranged by size:
4636 777 573 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2409 2670 2685 4293 4378 5449 2247
Size: 162 261 15 1608 85 1071 3030
Fragments arranged by size:
3030 1608 1071 261 162 85 15
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2120 2178 2247 2274 2409 2670
Size: 399 312 18 58 69 27 135 261
Cuts at: 2670 2685 2705 3215 3383 3551 4293 4378 5449
Size: 15 20 510 168 168 742 85 1071
Cuts at: 5449 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 510 399 312 261 168
168 135 85 69 58 27 20 18
15
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 2025 4121 5851 2025
Size: 2096 1730 2406
Fragments arranged by size:
2406 2096 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2904 3587 844
Size: 2060 683 3489
Fragments arranged by size:
3489 2060 683
BsrBI CCG'CTC
Cuts at: 1394 1823 2482 3315 3419 4119 1394
Size: 429 659 833 104 700 3507
Fragments arranged by size:
3507 833 700 659 429 104
BsrDI GCAATG_nn'
Cuts at: 65 1248 4727 4901 65
Size: 1183 3479 174 1396
Fragments arranged by size:
3479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2278 2923 3064 3112 3383 4876 5063
Size: 110 468 645 141 48 271 1493 187
Cuts at: 5063 1700
Size: 2869
Fragments arranged by size:
2869 1493 645 468 271 187 141 110
48
BsrGI T'GTAC_A
Cuts at: 96 3432 96
Size: 3336 2896
BssHII G'CGCG_C
Cuts at: 1925 2360 1925
Size: 435 5797
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 2941 1184
Size: 1757 4475
BstYI r'GATC_y
Cuts at: 1972 3562 3929 4314 4331 5783 5869 5881 6227
Size: 1590 367 385 17 1452 86 12 346
Cuts at: 6227 1972
Size: 1977
Fragments arranged by size:
1977 1590 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2531 2580 2976 513
Size: 552 1029 437 49 396 3769
Fragments arranged by size:
3769 1029 552 437 396 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3427 487
Size: 683 56 2201 3292
Fragments arranged by size:
3292 2201 683 56
BtsI GCAGTG_nn'
Cuts at: 3748 4542 4562 3748
Size: 794 20 5418
Fragments arranged by size:
5418 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 2925 2935
Size: 54 1425 50 275 18 1078 17 10
Cuts at: 2935 2979 3679 4568 6197 8
Size: 44 700 889 1629 43
Fragments arranged by size:
1629 1425 1078 889 700 275 54 50
44 43 18 17 10
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4162 1353
Size: 2809 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 2551 2579 4819 5193 5339
Size: 1064 138 903 59 28 2240 374 146
Cuts at: 5339 387
Size: 1280
Fragments arranged by size:
2240 1280 1064 903 374 146 138 59
28
EcoICRI GAG'CTC
Cuts at: 2407 2407
Size: 6232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2281 2452 2780 3331 4295 4892
Size: 393 572 77 171 328 551 964 597
Cuts at: 4892 5679 5947 1239
Size: 787 268 1524
Fragments arranged by size:
1524 964 787 597 572 551 393 328
268 171 77
EcoO109I rG'GnC_Cy
Cuts at: 2053 2701 3058 3460 2053
Size: 648 357 402 4825
Fragments arranged by size:
4825 648 402 357
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 6232
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2067 2624 2776 3029 3080 3101 3427 5379
Size: 389 557 152 253 51 21 326 1952
Cuts at: 5379 1678
Size: 2531
Fragments arranged by size:
2531 1952 557 389 326 253 152 51
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 4873 4905 4947
Size: 32 135 32 1265 32 2004 32 42
Cuts at: 4947 4979 1373
Size: 32 2626
Fragments arranged by size:
2626 2004 1265 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 2878 3576 3822 669
Size: 988 281 352 588 698 246 3079
Fragments arranged by size:
3079 988 698 588 352 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KasI G'GCGC_C
Cuts at: 2063 2772 3025 3076 3097 2063
Size: 709 253 51 21 5198
Fragments arranged by size:
5198 709 253 51 21
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 5346 5530 1305
Size: 1575 2466 184 2007
Fragments arranged by size:
2466 2007 1575 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4410
Fragments arranged by size:
4410 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2279 3407 4190 4549 4708 518
Size: 648 864 249 1128 783 359 159 2042
Fragments arranged by size:
2042 1128 864 783 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2533 2978 3208 3648 4325 5473 5718
Size: 490 437 445 230 440 677 1148 245
Cuts at: 5718 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 490 445 440 437 245
230
NaeI GCC'GGC
Cuts at: 2925 3066 3114 2925
Size: 141 48 6043
Fragments arranged by size:
6043 141 48
NarI GG'CG_CC
Cuts at: 2064 2773 3026 3077 3098 2064
Size: 709 253 51 21 5198
Fragments arranged by size:
5198 709 253 51 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5680
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NgoMIV G'CCGG_C
Cuts at: 2923 3064 3112 2923
Size: 141 48 6043
Fragments arranged by size:
6043 141 48
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3590 5135 1132
Size: 97 2361 1545 2229
Fragments arranged by size:
2361 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2698 1348
Size: 1350 4882
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PfoI T'CCnGG_A
Cuts at: 2013 2013
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PmlI CAC'GTG
Cuts at: 3004 3004
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2176 2192 2224 3335 3367 4301 4333 5814
Size: 32 16 32 1111 32 934 32 1481
Cuts at: 5814 5846 2144
Size: 32 2530
Fragments arranged by size:
2530 1481 1111 934 32 32 32 32
32 16
PpuMI rG'GwC_Cy
Cuts at: 3460 3460
Size: 6232
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 2701 2701
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2628 3584 830
Size: 1798 956 3478
Fragments arranged by size:
3478 1798 956
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 3208 3208
Size: 6232
SacI G_AGCT'C
Cuts at: 2409 2409
Size: 6232
SacII CC_GC'GG
Cuts at: 2097 2534 2979 2097
Size: 437 445 5350
Fragments arranged by size:
5350 445 437
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SanDI GG'GwC_CC
Cuts at: 3460 3460
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2624 3223 3262 3286 3358 3580 4715
Size: 219 1579 599 39 24 72 222 1135
Cuts at: 4715 5396 5587 826
Size: 681 191 1471
Fragments arranged by size:
1579 1471 1135 681 599 222 219 191
72 39 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 3065 3065
Size: 6232
SfoI GGC'GCC
Cuts at: 2065 2774 3027 3078 3099 2065
Size: 709 253 51 21 5198
Fragments arranged by size:
5198 709 253 51 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 2916 3559 1872
Size: 1044 643 4545
Fragments arranged by size:
4545 1044 643
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 5237 5499 820
Size: 197 926 2393 901 262 1553
Fragments arranged by size:
2393 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2166 3671 513
Size: 552 1101 1505 3074
Fragments arranged by size:
3074 1505 1101 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 2731 4259 4276 4429 4614 5059
Size: 1255 471 680 1528 17 153 185 445
Cuts at: 5059 325
Size: 1498
Fragments arranged by size:
1528 1498 1255 680 471 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3432 4478 6190
Size: 274 80 33 51 1443 1455 1046 1712
Cuts at: 6190 96
Size: 138
Fragments arranged by size:
1712 1455 1443 1046 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2633 2665 3329 3361 6192 6224 2633
Size: 32 664 32 2831 32 2641
Fragments arranged by size:
2831 2641 664 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 2914 3557 1870
Size: 1044 643 4545
Fragments arranged by size:
4545 1044 643
XmnI GAAnn'nnTTC
Cuts at: 2159 2336 4361 2159
Size: 177 2025 4030
Fragments arranged by size:
4030 2025 177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2848 4040 276
Size: 53 83 186 936 1314 1192 2468
Fragments arranged by size:
2468 1314 1192 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SapI SexAI SrfI SwaI