Restriction map for
pFN21AB3938
(Circular) (Six-base) MAPSORT of: pFN21AB3938.seq Check: 9995 from: 1 to: 6031
pFN21AB3938 6031 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2215 2215
Size: 6031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 3374 1937
Size: 544 893 4594
Fragments arranged by size:
4594 893 544
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2730 3059 4094 5478 5746 1632
Size: 1098 329 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1098 1035 329 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 4876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2628 2660 2628
Size: 32 5999
AlwNI CAG_nnn'CTG
Cuts at: 3774 5346 3774
Size: 1572 4459
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 3136 3340 3566 3718 5827 1493
Size: 1643 204 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 226 204 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 3356 1870
Size: 73 1413 4545
Fragments arranged by size:
4545 1413 73
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2203 2215 2533 3216 3352
Size: 325 217 627 416 12 318 683 136
Cuts at: 3352 4807 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 683 627 416 325 318 217
136 12
BanII G_rGCy'C
Cuts at: 1391 3350 1391
Size: 1959 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2795 928
Size: 971 896 4164
Fragments arranged by size:
4164 971 896
BbvCI CC'TCA_GC
Cuts at: 3026 3026
Size: 6031
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4222 4256 1903
Size: 34 50 34 2201 34 3678
Fragments arranged by size:
3678 2201 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 2941 3283 1548
Size: 1393 342 4296
Fragments arranged by size:
4296 1393 342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 2432 4642
Size: 107 122 71 631 605 517 243 2210
Cuts at: 4642 136
Size: 1525
Fragments arranged by size:
2210 1525 631 605 517 243 122 107
71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 3448 3448
Size: 6031
Bme1580I G_kGCm'C
Cuts at: 1790 4092 5248 1790
Size: 2302 1156 2573
Fragments arranged by size:
2573 2302 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3762 4720 442
Size: 416 53 83 399 24 2345 958 1753
Fragments arranged by size:
2345 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4691 1239
Size: 3452 2579
Bpu10I CC'TnA_GC
Cuts at: 3026 3026
Size: 6031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4156 5021 5319 4156
Size: 865 298 4868
Fragments arranged by size:
4868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2436 2692 4694 882
Size: 617 937 256 2002 2219
Fragments arranged by size:
2219 2002 937 617 256
BsaAI yAC'GTr
Cuts at: 493 1204 3822 493
Size: 711 2618 2702
Fragments arranged by size:
2702 2618 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2144 3838 4220 4855
Size: 53 83 186 936 611 1694 382 635
Cuts at: 4855 275
Size: 1451
Fragments arranged by size:
1694 1451 936 635 611 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5806
Fragments arranged by size:
5806 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 5118
BseYI C'CCAG_C
Cuts at: 2344 5234 5730 2344
Size: 2890 496 2645
Fragments arranged by size:
2890 2645 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 1163
Size: 573 699 4759
Fragments arranged by size:
4759 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2198 3481 4242 5270 1540
Size: 275 186 197 1283 761 1028 2301
Fragments arranged by size:
2301 1283 1028 761 275 197 186
BsiHKAI G_wGCw'C
Cuts at: 2587 4092 4177 5248 2587
Size: 1505 85 1071 3370
Fragments arranged by size:
3370 1505 1071 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2950 2950
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2587 3350 4092 4177 5248 1391
Size: 399 797 763 742 85 1071 2174
Fragments arranged by size:
2174 1071 797 763 742 399 85
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2454 3386 844
Size: 1371 239 932 3489
Fragments arranged by size:
3489 1371 932 239
BsrBI CCG'CTC
Cuts at: 1394 1823 3918 1394
Size: 429 2095 3507
Fragments arranged by size:
3507 2095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4526 4700 65
Size: 1183 3278 174 1396
Fragments arranged by size:
3278 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4675 4862 1700
Size: 110 2865 187 2869
Fragments arranged by size:
2869 2865 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6031
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 2073 2240 3361 3728 4113 4130 5582 5668
Size: 101 167 1121 367 385 17 1452 86
Cuts at: 5668 5680 6026 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1121 385 367 346 167 101
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2556 513
Size: 552 1491 3988
Fragments arranged by size:
3988 1491 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 2868 3547 4341 4361 2868
Size: 679 794 20 4538
Fragments arranged by size:
4538 794 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 2937 3335 2937
Size: 398 5633
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2504 2862 2967
Size: 54 1425 50 275 18 674 358 105
Cuts at: 2967 3478 4367 5996 8
Size: 511 889 1629 43
Fragments arranged by size:
1629 1425 889 674 511 358 275 105
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3478 1537
Size: 275 1666 4090
Fragments arranged by size:
4090 1666 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3961 1353
Size: 2608 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2235 4618 4992 5138 387
Size: 1064 138 646 2383 374 146 1280
Fragments arranged by size:
2383 1280 1064 646 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2730 3059 4094 4691 5478 5746 1239
Size: 393 1098 329 1035 597 787 268 1524
Fragments arranged by size:
1524 1098 1035 787 597 393 329 268
EcoO109I rG'GnC_Cy
Cuts at: 2279 2279
Size: 6031
EcoRI G'AATT_C
Cuts at: 3136 3340 3718 3136
Size: 204 378 5449
Fragments arranged by size:
5449 378 204
EcoRV GAT'ATC
Cuts at: 2603 2603
Size: 6031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 6031
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 5178 1678
Size: 3500 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373
Size: 32 135 32 3100 32 42 32 2626
Fragments arranged by size:
3100 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3153 3630 3153
Size: 477 5554
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 5145 5329 1305
Size: 897 2943 184 2007
Fragments arranged by size:
2943 2007 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2506 2864 2969 10
Size: 54 1425 343 674 358 105 3072
Fragments arranged by size:
3072 1425 674 358 343 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 3205 3291 3989 4348 4507 518
Size: 648 1155 884 86 698 359 159 2042
Fragments arranged by size:
2042 1155 884 698 648 359 159 86
MspA1I CmG'CkG
Cuts at: 1606 3447 4124 5272 5517 1606
Size: 1841 677 1148 245 2120
Fragments arranged by size:
2120 1841 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5479
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 2401 2926 3389 4934 1132
Size: 97 1172 525 463 1545 2229
Fragments arranged by size:
2229 1545 1172 525 463 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6031
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2279 2279
Size: 6031
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3054 3601 3054
Size: 547 5484
PstI C_TGCA'G
Cuts at: 830 3383 830
Size: 2553 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 3379 4514 5195 5386 826
Size: 219 2334 1135 681 191 1471
Fragments arranged by size:
2334 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4135 5036 5298 820
Size: 197 926 2192 901 262 1553
Fragments arranged by size:
2192 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 2401 2926 3389 1132
Size: 1269 525 463 3774
Fragments arranged by size:
3774 1269 525 463
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2169 2275 3243 3470 513
Size: 552 1104 106 968 227 3074
Fragments arranged by size:
3074 1104 968 552 227 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 2671 4058 4075 4228 4413 4858
Size: 1255 584 507 1387 17 153 185 445
Cuts at: 4858 325
Size: 1498
Fragments arranged by size:
1498 1387 1255 584 507 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4277 5989 96
Size: 274 80 33 51 1443 2300 1712 138
Fragments arranged by size:
2300 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2553 2585 5991 6023 2553
Size: 32 3406 32 2561
Fragments arranged by size:
3406 2561 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2434 2185
Size: 249 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI