Restriction map for
pFN21AB3935
(Circular) (Six-base) MAPSORT of: pFN21AB3935.seq Check: 2182 from: 1 to: 5971
pFN21AB3935 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3064 3064
Size: 5971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 2647 3292 2647
Size: 645 5326
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2426 2924 2996 4034 5418 5686 1632
Size: 521 273 498 72 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1038 521 498 273 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2482 3714 5286 2482
Size: 1232 1572 3167
Fragments arranged by size:
3167 1572 1232
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2163 2582 2732 3280 3506 3658 5767 1493
Size: 670 419 150 548 226 152 2109 1697
Fragments arranged by size:
2109 1697 670 548 419 226 152 150
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2647 3292 4747 618
Size: 325 217 627 860 645 1455 1842
Fragments arranged by size:
1842 1455 860 645 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3290 1391
Size: 1899 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 2614 928
Size: 971 446 269 4285
Fragments arranged by size:
4285 971 446 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2050 2084 4162 4196 1903
Size: 34 113 34 2078 34 3678
Fragments arranged by size:
3678 2078 113 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BfrBI ATG'CAT
Cuts at: 2120 2120
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3257 4582 136
Size: 107 122 71 631 605 1585 1325 1525
Fragments arranged by size:
1585 1525 1325 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2534 4032 5188 1790
Size: 744 1498 1156 2573
Fragments arranged by size:
2573 1498 1156 744
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 3208 3702
Size: 416 53 83 399 24 1024 767 494
Cuts at: 3702 4660 442
Size: 958 1753
Fragments arranged by size:
1753 1024 958 767 494 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 2909 4631 1239
Size: 882 788 1722 2579
Fragments arranged by size:
2579 1722 882 788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 4096 4961 5259 2345
Size: 1751 865 298 3057
Fragments arranged by size:
3057 1751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2548 2854 3762 493
Size: 711 1344 306 908 2702
Fragments arranged by size:
2702 1344 908 711 306
BsaBI GATnn'nnATC
Cuts at: 2700 5965 2700
Size: 3265 2706
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 3251 1186
Size: 1252 813 3906
Fragments arranged by size:
3906 1252 813
BseYI C'CCAG_C
Cuts at: 5174 5670 5174
Size: 496 5475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3092 1163
Size: 573 1356 4042
Fragments arranged by size:
4042 1356 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4032 4117 5188 4032
Size: 85 1071 4815
Fragments arranged by size:
4815 1071 85
BsmI GAATG_Cn'
Cuts at: 2308 3482 3575 2308
Size: 1174 93 4704
Fragments arranged by size:
4704 1174 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2534 3290 4032 4117 5188 1391
Size: 399 744 756 742 85 1071 2174
Fragments arranged by size:
2174 1071 756 744 742 399 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 2799 3064 3220 3326 844
Size: 1390 565 265 156 106 3489
Fragments arranged by size:
3489 1390 565 265 156 106
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 2168 4466 4640 65
Size: 1183 835 85 2298 174 1396
Fragments arranged by size:
2298 1396 1183 835 174 85
BsrFI r'CCGG_y
Cuts at: 1700 1810 3155 4615 4802 1700
Size: 110 1345 1460 187 2869
Fragments arranged by size:
2869 1460 1345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3226 794
Size: 2432 3539
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966
Size: 1329 367 385 17 1452 86 12 346
Cuts at: 5966 1972
Size: 1977
Fragments arranged by size:
1977 1452 1329 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2177 513
Size: 552 939 173 4307
Fragments arranged by size:
4307 939 552 173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 2737 3275 2737
Size: 538 5433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3189 3258
Size: 54 1425 50 275 18 934 425 69
Cuts at: 3258 3418 4307 5936 8
Size: 160 889 1629 43
Fragments arranged by size:
1629 1425 934 889 425 275 160 69
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 2805 3901 1353
Size: 1062 390 1096 3423
Fragments arranged by size:
3423 1096 1062 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2153 2426 2909 2924 2996 4034
Size: 393 489 32 273 483 15 72 1038
Cuts at: 4034 4631 5418 5686 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1038 787 597 489 483 393 273
268 72 32 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2103 2103
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 2029 2356 2029
Size: 327 5644
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
EcoRV GAT'ATC
Cuts at: 2833 2833
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2335 2367 2693
Size: 32 135 32 430 32 301 32 326
Cuts at: 2693 2725 4612 4644 4686 4718 1373
Size: 32 1887 32 42 32 2626
Fragments arranged by size:
2626 1887 430 326 301 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 2651 3296 2651
Size: 645 5326
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 3191 10
Size: 54 1425 343 934 425 2790
Fragments arranged by size:
2790 1425 934 425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 3929 4288 4447 518
Size: 648 1146 1617 359 159 2042
Fragments arranged by size:
2042 1617 1146 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2479 3387 4064 5212 5457 1606
Size: 873 908 677 1148 245 2120
Fragments arranged by size:
2120 1148 908 873 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NruI TCG'CGA
Cuts at: 2060 2060
Size: 5971
NsiI A_TGCA'T
Cuts at: 2122 2122
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3187 3329 4874 1132
Size: 97 1958 142 1545 2229
Fragments arranged by size:
2229 1958 1545 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 1108
Size: 1374 4597
PfoI T'CCnGG_A
Cuts at: 2098 2098
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2594 2879 3175 1613
Size: 981 285 296 4409
Fragments arranged by size:
4409 981 296 285
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3231 3323 830
Size: 2401 92 3478
Fragments arranged by size:
3478 2401 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2479 2479
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SapI GCTCTTCn'nnn_
Cuts at: 2805 2805
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 3231 3323 3231
Size: 92 5879
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 3227 3319 4454 5135 5326 826
Size: 219 2182 92 1135 681 191 1471
Fragments arranged by size:
2182 1471 1135 681 219 191 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 3155 1700
Size: 1455 4516
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 4075 4976 5238 820
Size: 197 926 417 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 417 262 197
SnaBI TAC'GTA
Cuts at: 493 2854 3762 493
Size: 2361 908 2702
Fragments arranged by size:
2702 2361 908
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 3410 513
Size: 552 939 513 893 3074
Fragments arranged by size:
3074 939 893 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2654 2816 2909 3998 4015 4168 4353
Size: 1255 1074 162 93 1089 17 153 185
Cuts at: 4353 4798 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1089 1074 445 185 162 153
93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2524 2556 5931 5963 2524
Size: 32 3375 32 2532
Fragments arranged by size:
3375 2532 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2175 2175
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI