Restriction map for
pFN21AB3917
(Circular) (Six-base) MAPSORT of: pFN21AB3917.seq Check: 6122 from: 1 to: 9865
pFN21AB3917 9865 bp
With 182 enzymes: SgfI *
March 4, 2015 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2210 2210
Size: 9865
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5901 7675 278
Size: 53 83 186 936 4365 1774 2468
Fragments arranged by size:
4365 2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 6912 7208 1937
Size: 472 4503 296 4594
Fragments arranged by size:
4594 4503 472 296
Acc65I G'GTAC_C
Cuts at: 7186 7186
Size: 9865
AclI AA'CG_TT
Cuts at: 1563 7992 8365 1563
Size: 6429 373 3063
Fragments arranged by size:
6429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2091 2200 3170 3735 4052 4613 5194 5618
Size: 459 109 970 565 317 561 581 424
Cuts at: 5618 5849 5867 6234 7067 7928 9312 9580 1632
Size: 231 18 367 833 861 1384 268 1917
Fragments arranged by size:
1917 1384 970 861 833 581 565 561
459 424 367 317 268 231 109 18
AfeI AGC'GCT
Cuts at: 2615 3529 5609 2615
Size: 914 2080 6871
Fragments arranged by size:
6871 2080 914
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9668
AflIII A'CryG_T
Cuts at: 1203 2542 2553 3348 6834 8764 9741 1203
Size: 1339 11 795 3486 1930 977 1327
Fragments arranged by size:
3486 1930 1339 1327 977 795 11
AgeI A'CCGG_T
Cuts at: 8696 8696
Size: 9865
AhdI GACnn_n'nnGTC
Cuts at: 8594 8594
Size: 9865
AleI CACnn'nnGTG
Cuts at: 1166 2204 4940 5864 1166
Size: 1038 2736 924 5167
Fragments arranged by size:
5167 2736 1038 924
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4823 5063 1184
Size: 3639 240 5986
Fragments arranged by size:
5986 3639 240
AlwNI CAG_nnn'CTG
Cuts at: 2223 3265 3571 5056 5098 5968 7045 7159 7608
Size: 1042 306 1485 42 870 1077 114 449
Cuts at: 7608 9180 2223
Size: 1572 2908
Fragments arranged by size:
2908 1572 1485 1077 1042 870 449 306
114 42
ApaI G_GGCC'C
Cuts at: 2174 2398 4476 6489 2174
Size: 224 2078 2013 5550
Fragments arranged by size:
5550 2078 2013 224
ApaLI G'TGCA_C
Cuts at: 2696 3473 7922 9078 2696
Size: 777 4449 1156 3483
Fragments arranged by size:
4449 3483 1156 777
ApoI r'AATT_y
Cuts at: 1493 2424 2472 3375 5289 5936 7174 7400 7552
Size: 931 48 903 1914 647 1238 226 152
Cuts at: 7552 9661 1493
Size: 2109 1697
Fragments arranged by size:
2109 1914 1697 1238 931 903 647 226
152 48
AseI AT'TA_AT
Cuts at: 160 1032 8419 160
Size: 872 7387 1606
Fragments arranged by size:
7387 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9865
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 3933 3951 6008 6488 7190 1870
Size: 73 400 1590 18 2057 480 702 4545
Fragments arranged by size:
4545 2057 1590 702 480 400 73 18
AvrII C'CTAG_G
Cuts at: 2947 2947
Size: 9865
BamHI G'GATC_C
Cuts at: 6987 7067 7195 7562 6987
Size: 80 128 367 9290
Fragments arranged by size:
9290 367 128 80
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 2914 3589 5264 6576
Size: 325 217 627 445 682 675 1675 1312
Cuts at: 6576 6653 6687 7186 8641 618
Size: 77 34 499 1455 1842
Fragments arranged by size:
1842 1675 1455 1312 682 675 627 499
445 325 217 77 34
BanII G_rGCy'C
Cuts at: 1391 2079 2174 2253 2312 2398 2730 4274 4476
Size: 688 95 79 59 86 332 1544 202
Cuts at: 4476 5670 6480 6489 6629 7149 7184 1391
Size: 1194 810 9 140 520 35 4072
Fragments arranged by size:
4072 1544 1194 810 688 520 332 202
140 95 86 79 59 35 9
BbeI G_GCGC'C
Cuts at: 5268 5268
Size: 9865
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4410 6339 928
Size: 971 2511 1929 4454
Fragments arranged by size:
4454 2511 1929 971
BbvCI CC'TCA_GC
Cuts at: 2375 5366 6219 7082 2375
Size: 2991 853 863 5158
Fragments arranged by size:
5158 2991 863 853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2035 2069 2444 2478 3770 3804 6892
Size: 34 98 34 375 34 1292 34 3088
Cuts at: 6892 6926 8056 8090 1903
Size: 34 1130 34 3678
Fragments arranged by size:
3678 3088 1292 1130 375 98 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2759 7757 8967 2759
Size: 4998 1210 3657
Fragments arranged by size:
4998 3657 1210
BclI T'GATC_A
Cuts at: 1548 3847 4215 1548
Size: 2299 368 7198
Fragments arranged by size:
7198 2299 368
BfrBI ATG'CAT
Cuts at: 4644 5978 4644
Size: 1334 8531
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 3587 4752
Size: 107 122 71 631 605 360 1555 1165
Cuts at: 4752 4959 6484 8476 136
Size: 207 1525 1992 1525
Fragments arranged by size:
1992 1555 1525 1525 1165 631 605 360
207 122 107 71
BglII A'GATC_T
Cuts at: 4305 4737 9860 4305
Size: 432 5123 4310
Fragments arranged by size:
5123 4310 432
BlpI GC'TnA_GC
Cuts at: 2610 7282 2610
Size: 4672 5193
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2235 2398 2700 2788 3477 3592 3703
Size: 384 61 163 302 88 689 115 111
Cuts at: 3703 4476 5805 6201 6489 7926 9082 1790
Size: 773 1329 396 288 1437 1156 2573
Fragments arranged by size:
2573 1437 1329 1156 773 689 396 384
302 288 163 115 111 88 61
BmgBI CAC'GTC
Cuts at: 1318 3196 1318
Size: 1878 7987
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2880 2930 4149
Size: 416 53 83 399 24 1463 50 1219
Cuts at: 4149 5433 7596 8554 442
Size: 1284 2163 958 1753
Fragments arranged by size:
2163 1753 1463 1284 1219 958 416 399
83 53 50 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 9865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3287 8525 1239
Size: 2048 5238 2579
Fragments arranged by size:
5238 2579 2048
Bpu10I CC'TnA_GC
Cuts at: 2375 3524 3594 4957 5366 6219 6406 7082 2375
Size: 1149 70 1363 409 853 187 676 5158
Fragments arranged by size:
5158 1363 1149 853 676 409 187 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2438 2908 5293 5339 7133 7990 8855 9153
Size: 375 470 2385 46 1794 857 865 298
Cuts at: 9153 2063
Size: 2775
Fragments arranged by size:
2775 2385 1794 865 857 470 375 298
46
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2946 4313 4771 5998 7092 8528 882
Size: 617 1447 1367 458 1227 1094 1436 2219
Fragments arranged by size:
2219 1447 1436 1367 1227 1094 617 458
BsaAI yAC'GTr
Cuts at: 493 1204 6837 7656 493
Size: 711 5633 819 2702
Fragments arranged by size:
5633 2702 819 711
BsaBI GATnn'nnATC
Cuts at: 4551 4736 6608 9859 4551
Size: 185 1872 3251 4557
Fragments arranged by size:
4557 3251 1872 185
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4793 5265 5455 5898
Size: 53 83 186 936 3260 472 190 443
Cuts at: 5898 6231 7672 8054 8689 275
Size: 333 1441 382 635 1451
Fragments arranged by size:
3260 1451 1441 936 635 472 443 382
333 190 186 83 53
BsaWI w'CCGG_w
Cuts at: 6990 8298 8696 8970 9117 9528 6990
Size: 1308 398 274 147 411 7327
Fragments arranged by size:
7327 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3665 3695 3959 3989 5145
Size: 30 165 30 2262 30 264 30 1156
Cuts at: 5145 5175 6410 6440 6557 6587 6611 6641 1178
Size: 30 1235 30 117 30 24 30 4402
Fragments arranged by size:
4402 2262 1235 1156 264 165 117 30
30 30 30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 4155 6172 6206 6236 6284 6287 6314
Size: 1081 1888 2017 34 30 48 3 27
Cuts at: 6314 6317 6332 6431 6578 1186
Size: 3 15 99 147 4473
Fragments arranged by size:
4473 2017 1888 1081 147 99 48 34
30 27 15 3 3
BseYI C'CCAG_C
Cuts at: 3543 3577 3811 4750 6143 7063 9068 9564 3543
Size: 34 234 939 1393 920 2005 496 3844
Fragments arranged by size:
3844 2005 1393 939 920 496 234 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 2531 3656 3826 4718 5027 5069
Size: 573 492 303 1125 170 892 309 42
Cuts at: 5069 1163
Size: 5959
Fragments arranged by size:
5959 1125 892 573 492 309 303 170
42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5424 6912 7315 8076 9104 1540
Size: 275 186 3423 1488 403 761 1028 2301
Fragments arranged by size:
3423 2301 1488 1028 761 403 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2198 2253 2700 3477 3960 4274 5811 6373
Size: 119 55 447 777 483 314 1537 562
Cuts at: 6373 6629 7149 7926 8011 9082 2079
Size: 256 520 777 85 1071 2862
Fragments arranged by size:
2862 1537 1071 777 777 562 520 483
447 314 256 119 85 55
BsmI GAATG_Cn'
Cuts at: 2611 3793 5736 7376 7469 2611
Size: 1182 1943 1640 93 5007
Fragments arranged by size:
5007 1943 1640 1182 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 9865
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2174 2198 2235 2253 2312 2398
Size: 399 289 95 24 37 18 59 86
Cuts at: 2398 2700 2730 2788 3477 3592 3703 3960 4274
Size: 302 30 58 689 115 111 257 314
Cuts at: 4274 4476 5670 5805 5811 6201 6373 6480 6489
Size: 202 1194 135 6 390 172 107 9
Cuts at: 6489 6629 7149 7184 7926 8011 9082 1391
Size: 140 520 35 742 85 1071 2174
Fragments arranged by size:
2174 1194 1071 742 689 520 399 390
314 302 289 257 202 172 140 135
115 111 107 95 86 85 59 58
37 35 30 24 18 9 6
BspEI T'CCGG_A
Cuts at: 9528 9528
Size: 9865
BspHI T'CATG_A
Cuts at: 2997 7754 9484 2997
Size: 4757 1730 3378
Fragments arranged by size:
4757 3378 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2210 2304 4349 4377 4509 4854 7220 844
Size: 1366 94 2045 28 132 345 2366 3489
Fragments arranged by size:
3489 2366 2045 1366 345 132 94 28
BsrBI CCG'CTC
Cuts at: 1394 1823 4956 7752 1394
Size: 429 3133 2796 3507
Fragments arranged by size:
3507 3133 2796 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 4630 5341 8360 8534 65
Size: 1183 1329 2053 711 3019 174 1396
Fragments arranged by size:
3019 2053 1396 1329 1183 711 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3621 4874 8509 8696 1700
Size: 110 1811 1253 3635 187 2869
Fragments arranged by size:
3635 2869 1811 1253 187 110
BsrGI T'GTAC_A
Cuts at: 96 3351 96
Size: 3255 6610
BssHII G'CGCG_C
Cuts at: 1925 3326 1925
Size: 1401 8464
BssSI C'ACGA_G
Cuts at: 4741 7925 8937 4741
Size: 3184 1012 5669
Fragments arranged by size:
5669 3184 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9865
BstBI TT'CG_AA
Cuts at: 3019 8669 3019
Size: 5650 4215
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9865
BstXI CCAn_nnnn'nTGG
Cuts at: 7063 7063
Size: 9865
BstYI r'GATC_y
Cuts at: 1972 3261 4305 4737 5185 6130 6525 6790 6987
Size: 1289 1044 432 448 945 395 265 197
Cuts at: 6987 7067 7195 7562 7947 7964 9416 9502 9514
Size: 80 128 367 385 17 1452 86 12
Cuts at: 9514 9860 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1289 1044 945 448 432 395
385 367 346 265 197 128 86 80
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2691 3381 3425 3489 3555 3582 6495
Size: 552 1626 690 44 64 66 27 2913
Cuts at: 6495 6649 513
Size: 154 3729
Fragments arranged by size:
3729 2913 1626 690 552 154 66 64
44 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6889 487
Size: 683 56 5663 3463
Fragments arranged by size:
5663 3463 683 56
BtsI GCAGTG_nn'
Cuts at: 2623 3668 4291 4465 4805 4903 4935 5047 7381
Size: 1045 623 174 340 98 32 112 2334
Cuts at: 7381 8175 8195 2623
Size: 794 20 4293
Fragments arranged by size:
4293 2334 1045 794 623 340 174 112
98 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9830
DraI TTT'AAA
Cuts at: 6046 7169 6046
Size: 1123 8742
DraIII CAC_nnn'GTG
Cuts at: 5618 6205 6687 5618
Size: 587 482 8796
Fragments arranged by size:
8796 587 482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8872 809
Size: 507 7556 1802
Fragments arranged by size:
7556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2710 2971 3069
Size: 54 1425 50 275 18 880 261 98
Cuts at: 3069 3558 3874 5124 5338 5421 5766 7312 8201
Size: 489 316 1250 214 83 345 1546 889
Cuts at: 8201 9830 8
Size: 1629 43
Fragments arranged by size:
1629 1546 1425 1250 889 880 489 345
316 275 261 214 98 83 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5421 7312 1537
Size: 275 3609 1891 4090
Fragments arranged by size:
4090 3609 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2285 2591 3711 4361 5254 5537 5825
Size: 714 218 306 1120 650 893 283 288
Cuts at: 5825 6109 6183 6291 6390 7006 7795 1353
Size: 284 74 108 99 616 789 3423
Fragments arranged by size:
3423 1120 893 789 714 650 616 306
288 284 283 218 108 99 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 8452 8826 8972 387
Size: 1064 138 2675 4188 374 146 1280
Fragments arranged by size:
4188 2675 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2251 4272 6627 7147 2077
Size: 174 2021 2355 520 4795
Fragments arranged by size:
4795 2355 2021 520 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2200 3170 3287 3735 4052 4613
Size: 393 459 109 970 117 448 317 561
Cuts at: 4613 5194 5618 5849 5867 6234 7067 7928 8525
Size: 581 424 231 18 367 833 861 597
Cuts at: 8525 9312 9580 1239
Size: 787 268 1524
Fragments arranged by size:
1524 970 861 833 787 597 581 561
459 448 424 393 367 317 268 231
117 109 18
EcoO109I rG'GnC_Cy
Cuts at: 2171 2394 2395 2529 4473 4924 5583 6421 6485
Size: 223 1 134 1944 451 659 838 64
Cuts at: 6485 6784 2171
Size: 299 5252
Fragments arranged by size:
5252 1944 838 659 451 299 223 134
64 1
EcoRI G'AATT_C
Cuts at: 2424 3375 7174 7552 2424
Size: 951 3799 378 4737
Fragments arranged by size:
4737 3799 951 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6631 6631
Size: 9865
FspI TGC'GCA
Cuts at: 8371 8371
Size: 9865
HaeII r_GCGC'y
Cuts at: 1678 2617 3531 5268 5611 5775 6505 9012 1678
Size: 939 914 1737 343 164 730 2507 2531
Fragments arranged by size:
2531 2507 1737 939 914 730 343 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2631 2663 3669 3701 3886
Size: 32 135 32 1059 32 1006 32 185
Cuts at: 3886 3918 6410 6442 6611 6643 7070 7102 8506
Size: 32 2492 32 169 32 427 32 1404
Cuts at: 8506 8538 8580 8612 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2492 1404 1059 1006 427 185 169
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2673 4071 6913 7209 7455 669
Size: 988 281 735 1398 2842 296 246 3079
Fragments arranged by size:
3079 2842 1398 988 735 296 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9865
HpaI GTT'AAC
Cuts at: 7455 7455
Size: 9865
KasI G'GCGC_C
Cuts at: 5264 5264
Size: 9865
KpnI G_GTAC'C
Cuts at: 7190 7190
Size: 9865
MfeI C'AATT_G
Cuts at: 7464 7464
Size: 9865
MluI A'CGCG_T
Cuts at: 9741 9741
Size: 9865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4754 5364 6834 8979 9163 1305
Size: 3449 610 1470 2145 184 2007
Fragments arranged by size:
3449 2145 2007 1470 610 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2712 2973 3071 3560 3876
Size: 54 1425 343 880 261 98 489 316
Cuts at: 3876 5126 5768 10
Size: 1250 642 4107
Fragments arranged by size:
4107 1425 1250 880 642 489 343 316
261 98 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2604 4412 4940 5163 5678 5864
Size: 648 1038 400 1808 528 223 515 186
Cuts at: 5864 7823 8182 8341 518
Size: 1959 359 159 2042
Fragments arranged by size:
2042 1959 1808 1038 648 528 515 400
359 223 186 159
MspA1I CmG'CkG
Cuts at: 1606 2374 2708 4045 4326 4786 5596 5953 6127
Size: 768 334 1337 281 460 810 357 174
Cuts at: 6127 7281 7958 9106 9351 1606
Size: 1154 677 1148 245 2120
Fragments arranged by size:
2120 1337 1154 1148 810 768 677 460
357 334 281 245 174
NaeI GCC'GGC
Cuts at: 3623 3623
Size: 9865
NarI GG'CG_CC
Cuts at: 5265 5265
Size: 9865
NcoI C'CATG_G
Cuts at: 513 1065 2691 3381 3425 3489 6495 6649 513
Size: 552 1626 690 44 64 3006 154 3729
Fragments arranged by size:
3729 3006 1626 690 552 154 64 44
NdeI CA'TA_TG
Cuts at: 387 2601 4519 387
Size: 2214 1918 5733
Fragments arranged by size:
5733 2214 1918
NgoMIV G'CCGG_C
Cuts at: 3621 3621
Size: 9865
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9865
NotI GC'GGCC_GC
Cuts at: 5421 7312 5421
Size: 1891 7974
NsiI A_TGCA'T
Cuts at: 4646 5980 4646
Size: 1334 8531
NspI r_CATG'y
Cuts at: 1132 1229 2026 2332 2546 3352 3629 4644 4648
Size: 97 797 306 214 806 277 1015 4
Cuts at: 4648 5978 6340 7223 8768 1132
Size: 1330 362 883 1545 2229
Fragments arranged by size:
2229 1545 1330 1015 883 806 797 362
306 277 214 97 4
PasI CC'CwG_GG
Cuts at: 1348 2533 2731 3601 4839 5348 5731 6481 1348
Size: 1185 198 870 1238 509 383 750 4732
Fragments arranged by size:
4732 1238 1185 870 750 509 383 198
PciI A'CATG_T
Cuts at: 2542 3348 8764 2542
Size: 806 5416 3643
Fragments arranged by size:
5416 3643 806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3883 4909 1108
Size: 2775 1026 6064
Fragments arranged by size:
6064 2775 1026
PfoI T'CCnGG_A
Cuts at: 3264 3897 5901 6786 3264
Size: 633 2004 885 6343
Fragments arranged by size:
6343 2004 885 633
PmeI GTTT'AAAC
Cuts at: 7169 7169
Size: 9865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7934 7966 9447 9479 7934
Size: 32 1481 32 8320
Fragments arranged by size:
8320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2529 5583 6421 6784 2529
Size: 3054 838 363 5610
Fragments arranged by size:
5610 3054 838 363
PshAI GACnn'nnGTC
Cuts at: 1613 5439 5514 1613
Size: 3826 75 5964
Fragments arranged by size:
5964 3826 75
PsiI TTA'TAA
Cuts at: 7435 7435
Size: 9865
PspOMI G'GGCC_C
Cuts at: 2170 2394 4472 6485 2170
Size: 224 2078 2013 5550
Fragments arranged by size:
5550 2078 2013 224
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2099 2131 2099
Size: 32 9833
PstI C_TGCA'G
Cuts at: 830 2043 2179 4374 4689 5103 5595 7217 830
Size: 1213 136 2195 315 414 492 1622 3478
Fragments arranged by size:
3478 2195 1622 1213 492 414 315 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9865
PvuII CAG'CTG
Cuts at: 2374 2708 5596 5953 2374
Size: 334 2888 357 6286
Fragments arranged by size:
6286 2888 357 334
RsrII CG'GwC_CG
Cuts at: 5023 5023
Size: 9865
SacI G_AGCT'C
Cuts at: 2079 2253 4274 6629 7149 2079
Size: 174 2021 2355 520 4795
Fragments arranged by size:
4795 2355 2021 520 174
SalI G'TCGA_C
Cuts at: 1936 6911 7207 1936
Size: 4975 296 4594
Fragments arranged by size:
4975 4594 296
SanDI GG'GwC_CC
Cuts at: 2529 2529
Size: 9865
SapI GCTCTTCn'nnn_
Cuts at: 2067 6109 2067
Size: 4042 5823
SbfI CC_TGCA'GG
Cuts at: 7217 7217
Size: 9865
ScaI AGT'ACT
Cuts at: 8113 9825 8113
Size: 1712 8153
SfcI C'TryA_G
Cuts at: 826 1045 2039 2175 3081 4370 4378 4685 5099
Size: 219 994 136 906 1289 8 307 414
Cuts at: 5099 5512 5591 7010 7213 8348 9029 9220 826
Size: 413 79 1419 203 1135 681 191 1471
Fragments arranged by size:
1471 1419 1289 1135 994 906 681 414
413 307 219 203 191 136 79 8
SfoI GGC'GCC
Cuts at: 5266 5266
Size: 9865
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9865
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9865
SmaI CCC'GGG
Cuts at: 1872 2345 3953 7192 1872
Size: 473 1608 3239 4545
Fragments arranged by size:
4545 3239 1608 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2417 2923 5272 5318 7148
Size: 197 926 135 339 506 2349 46 1830
Cuts at: 7148 7969 8870 9132 820
Size: 821 901 262 1553
Fragments arranged by size:
2349 1830 1553 926 901 821 506 339
262 197 135 46
SnaBI TAC'GTA
Cuts at: 493 7656 493
Size: 7163 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9865
SphI G_CATG'C
Cuts at: 1132 4644 4648 7223 1132
Size: 3512 4 2575 3774
Fragments arranged by size:
3774 3512 2575 4
SspI AAT'ATT
Cuts at: 5 52 3202 7789 5
Size: 47 3150 4587 2081
Fragments arranged by size:
4587 3150 2081 47
StuI AGG'CCT
Cuts at: 1517 3282 3755 3827 7137 1517
Size: 1765 473 72 3310 4245
Fragments arranged by size:
4245 3310 1765 473 72
StyI C'CwwG_G
Cuts at: 513 1065 2691 2947 3381 3425 3489 3867 4392
Size: 552 1626 256 434 44 64 378 525
Cuts at: 4392 5741 6495 6649 6672 7304 513
Size: 1349 754 154 23 632 3074
Fragments arranged by size:
3074 1626 1349 754 632 552 525 434
378 256 154 64 44 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2791 3091 3132 3492 4141 4478 5690
Size: 1255 1211 300 41 360 649 337 1212
Cuts at: 5690 5803 6479 7892 7909 8062 8247 8692 325
Size: 113 676 1413 17 153 185 445 1498
Fragments arranged by size:
1498 1413 1255 1212 1211 676 649 445
360 337 300 185 153 113 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3131 3351 8111
Size: 274 80 33 51 1443 1154 220 4760
Cuts at: 8111 9823 96
Size: 1712 138
Fragments arranged by size:
4760 1712 1443 1154 274 220 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3297 3329 3665 3697 5145 5177 6557 6589 9825
Size: 32 336 32 1448 32 1380 32 3236
Cuts at: 9825 9857 3297
Size: 32 3305
Fragments arranged by size:
3305 3236 1448 1380 336 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9865
XbaI T'CTAG_A
Cuts at: 2899 7201 2899
Size: 4302 5563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3423 5869 3423
Size: 2446 7419
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9865
XmaI C'CCGG_G
Cuts at: 1870 2343 3951 7190 1870
Size: 473 1608 3239 4545
Fragments arranged by size:
4545 3239 1608 473
XmnI GAAnn'nnTTC
Cuts at: 5936 7994 5936
Size: 2058 7807
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5899 7673 276
Size: 53 83 186 936 4365 1774 2468
Fragments arranged by size:
4365 2468 1774 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI BstZ17I Bsu36I EcoNI EcoRV
FseI FspAI NruI PacI PmlI PspXI SacII SexAI
SfiI SrfI SwaI