Restriction map for
pFN21AB3911
(Circular) (Six-base) MAPSORT of: pFN21AB3911.seq Check: 9894 from: 1 to: 6067
pFN21AB3911 6067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2466 4130 5514 5782 1632
Size: 834 1664 1384 268 1917
Fragments arranged by size:
1917 1664 1384 834 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 3810 5382 3810
Size: 1572 4495
ApaLI G'TGCA_C
Cuts at: 2068 4124 5280 2068
Size: 2056 1156 2855
Fragments arranged by size:
2855 2056 1156
ApoI r'AATT_y
Cuts at: 1493 2205 2615 2718 3044 3376 3602 3754 5863
Size: 712 410 103 326 332 226 152 2109
Cuts at: 5863 1493
Size: 1697
Fragments arranged by size:
2109 1697 712 410 332 326 226 152
103
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2709 3009 3392 1870
Size: 73 766 300 383 4545
Fragments arranged by size:
4545 766 383 300 73
BamHI G'GATC_C
Cuts at: 2230 3397 3764 2230
Size: 1167 367 4533
Fragments arranged by size:
4533 1167 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3386 1391
Size: 1995 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3221 928
Size: 971 1322 3774
Fragments arranged by size:
3774 1322 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2541 3221 3959 5169 2541
Size: 680 738 1210 3439
Fragments arranged by size:
3439 1210 738 680
BclI T'GATC_A
Cuts at: 1548 2800 1548
Size: 1252 4815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2072 4128 5284 1790
Size: 282 2056 1156 2573
Fragments arranged by size:
2573 2056 1156 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2746 4727 1239
Size: 1507 1981 2579
Fragments arranged by size:
2579 1981 1507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2412 4192 5057 5355 2412
Size: 1780 865 298 3124
Fragments arranged by size:
3124 1780 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4730 882
Size: 617 3231 2219
Fragments arranged by size:
3231 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3874 4256 4891 275
Size: 53 83 186 936 2341 382 635 1451
Fragments arranged by size:
2341 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5842
Fragments arranged by size:
5842 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 1186
Size: 853 5214
BseYI C'CCAG_C
Cuts at: 3309 5270 5766 3309
Size: 1961 496 3610
Fragments arranged by size:
3610 1961 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2493 3255 1163
Size: 573 757 762 3975
Fragments arranged by size:
3975 762 757 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 4128 4213 5284 2072
Size: 2056 85 1071 2855
Fragments arranged by size:
2855 2056 1071 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 3386 4128 4213 5284 1391
Size: 399 282 1314 742 85 1071 2174
Fragments arranged by size:
2174 1314 1071 742 399 282 85
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 2200 3956 5686 2200
Size: 1756 1730 2581
Fragments arranged by size:
2581 1756 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2359 3422 844
Size: 1515 1063 3489
Fragments arranged by size:
3489 1515 1063
BsrBI CCG'CTC
Cuts at: 1394 1823 3954 1394
Size: 429 2131 3507
Fragments arranged by size:
3507 2131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2278 2290 3192 96
Size: 2182 12 902 2971
Fragments arranged by size:
2971 2182 902 12
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2980 794
Size: 2186 3881
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2230 3397 3764 4149 4166 5618 5704 5716
Size: 258 1167 367 385 17 1452 86 12
Cuts at: 5716 6062 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1167 385 367 346 258 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3298 513
Size: 552 2233 3282
Fragments arranged by size:
3282 2233 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 2157 3371 2157
Size: 1214 4853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2956 3514 4403
Size: 54 1425 50 275 18 1126 558 889
Cuts at: 4403 6032 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1126 889 558 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3997 1353
Size: 2644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2466 2746 4130 4727 5514 5782 1239
Size: 393 834 280 1384 597 787 268 1524
Fragments arranged by size:
1524 1384 834 787 597 393 280 268
EcoRI G'AATT_C
Cuts at: 2615 3376 3754 2615
Size: 761 378 4928
Fragments arranged by size:
4928 761 378
EcoRV GAT'ATC
Cuts at: 2221 2221
Size: 6067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2429 2558 2429
Size: 129 5938
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2903 5214 1678
Size: 1225 2311 2531
Fragments arranged by size:
2531 2311 1225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2912 2944 4708 4740 4782
Size: 32 135 32 1340 32 1764 32 42
Cuts at: 4782 4814 1373
Size: 32 2626
Fragments arranged by size:
2626 1764 1340 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 2796 3666 2796
Size: 870 5197
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2989 5181 5365 1305
Size: 1684 2192 184 2007
Fragments arranged by size:
2192 2007 1684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2958 10
Size: 54 1425 343 1126 3119
Fragments arranged by size:
3119 1425 1126 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3079 4025 4384 4543 518
Size: 648 1913 946 359 159 2042
Fragments arranged by size:
2042 1913 946 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 2227 2701 3239 3483 4160 5308 5553
Size: 502 119 474 538 244 677 1148 245
Cuts at: 5553 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 538 502 474 245 244
119
NcoI C'CATG_G
Cuts at: 513 1065 3298 513
Size: 552 2233 3282
Fragments arranged by size:
3282 2233 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2568 1108
Size: 1460 4607
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 2864 3637 2864
Size: 773 5294
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2163 2195 2163
Size: 32 6035
PstI C_TGCA'G
Cuts at: 830 3419 830
Size: 2589 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2108 2227 2108
Size: 119 5948
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2180 2521 3415 4550 5231 5422 826
Size: 219 1135 341 894 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 894 681 341 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2427 3009 4171 5072 5334 820
Size: 197 926 484 582 1162 901 262 1553
Fragments arranged by size:
1553 1162 926 901 582 484 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2254 2308 2542 3298 3506 513
Size: 552 1189 54 234 756 208 3074
Fragments arranged by size:
3074 1189 756 552 234 208 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 2642 4094 4111 4264 4449 4894
Size: 1255 1004 58 1452 17 153 185 445
Cuts at: 4894 325
Size: 1498
Fragments arranged by size:
1498 1452 1255 1004 445 185 153 58
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2278 2290 2890
Size: 274 80 33 51 1443 301 12 600
Cuts at: 2890 3192 4313 6025 96
Size: 302 1121 1712 138
Fragments arranged by size:
1712 1443 1121 600 302 301 274 138
80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 2582 1316
Size: 1266 4801
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 3009 1943
Size: 1066 5001
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2615 4196 2615
Size: 1581 4486
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI