Restriction map for
pFN21AB3889
(Circular) (Six-base) MAPSORT of: pFN21AB3889.seq Check: 8783 from: 1 to: 7129
pFN21AB3889 7129 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3943 3943
Size: 7129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3104 4939 278
Size: 53 83 186 936 1568 1835 2468
Fragments arranged by size:
2468 1835 1568 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4472 1937
Size: 2535 4594
Acc65I G'GTAC_C
Cuts at: 3999 4450 3999
Size: 451 6678
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2596 2660 3320 3646 3725 5192 6576 6844
Size: 964 64 660 326 79 1467 1384 268
Cuts at: 6844 1632
Size: 1917
Fragments arranged by size:
1917 1467 1384 964 660 326 268 79
64
AfeI AGC'GCT
Cuts at: 3431 4272 3431
Size: 841 6288
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 6028 7005 1203
Size: 4825 977 1327
Fragments arranged by size:
4825 1327 977
AgeI A'CCGG_T
Cuts at: 2556 5960 2556
Size: 3404 3725
AhdI GACnn_n'nnGTC
Cuts at: 2256 5858 2256
Size: 3602 3527
AleI CACnn'nnGTG
Cuts at: 1166 3567 1166
Size: 2401 4728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3752 1184
Size: 2568 4561
AlwNI CAG_nnn'CTG
Cuts at: 2837 3544 3610 3739 4872 6444 2837
Size: 707 66 129 1133 1572 3522
Fragments arranged by size:
3522 1572 1133 707 129 66
ApaI G_GGCC'C
Cuts at: 2876 3039 3526 2876
Size: 163 487 6479
Fragments arranged by size:
6479 487 163
ApaLI G'TGCA_C
Cuts at: 2465 5186 6342 2465
Size: 2721 1156 3252
Fragments arranged by size:
3252 2721 1156
ApoI r'AATT_y
Cuts at: 1493 4438 4664 4816 6925 1493
Size: 2945 226 152 2109 1697
Fragments arranged by size:
2945 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 3038 3885 4235 4454 1870
Size: 73 221 874 847 350 219 4545
Fragments arranged by size:
4545 874 847 350 221 219 73
BamHI G'GATC_C
Cuts at: 4459 4826 4459
Size: 367 6762
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 3278 3632 3999 4450
Size: 325 217 627 348 1143 354 367 451
Cuts at: 4450 5905 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1143 627 451 367 354 348
325 217
BanII G_rGCy'C
Cuts at: 1391 2876 3039 3526 4448 1391
Size: 1485 163 487 922 4072
Fragments arranged by size:
4072 1485 922 487 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3544 928
Size: 971 1645 4513
Fragments arranged by size:
4513 1645 971
BbvCI CC'TCA_GC
Cuts at: 2225 3622 2225
Size: 1397 5732
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2683 2717 5320 5354 1903
Size: 34 746 34 2603 34 3678
Fragments arranged by size:
3678 2603 746 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5021 6231 5021
Size: 1210 5919
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2168 2261 2339
Size: 107 122 71 631 605 496 93 78
Cuts at: 2339 2348 5740 136
Size: 9 3392 1525
Fragments arranged by size:
3392 1525 631 605 496 122 107 93
78 71 9
BglII A'GATC_T
Cuts at: 2655 7124 2655
Size: 4469 2660
BlpI GC'TnA_GC
Cuts at: 2636 4316 4546 2636
Size: 1680 230 5219
Fragments arranged by size:
5219 1680 230
Bme1580I G_kGCm'C
Cuts at: 1790 2469 2876 3039 3281 3526 3652 3690 5190
Size: 679 407 163 242 245 126 38 1500
Cuts at: 5190 6346 1790
Size: 1156 2573
Fragments arranged by size:
2573 1500 1156 679 407 245 242 163
126 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3879 4860 5818
Size: 416 53 83 399 24 2462 981 958
Cuts at: 5818 442
Size: 1753
Fragments arranged by size:
2462 1753 981 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2378 1056
Size: 1322 5807
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 3911 2986
Size: 925 6204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 3232 3638 4313 5789 1239
Size: 968 1025 406 675 1476 2579
Fragments arranged by size:
2579 1476 1025 968 675 406
Bpu10I CC'TnA_GC
Cuts at: 2225 3249 3622 2225
Size: 1024 373 5732
Fragments arranged by size:
5732 1024 373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3656 4240 4295 5254 6119 6417 3656
Size: 584 55 959 865 298 4368
Fragments arranged by size:
4368 959 865 584 298 55
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 5792 882
Size: 617 507 3786 2219
Fragments arranged by size:
3786 2219 617 507
BsaAI yAC'GTr
Cuts at: 493 1204 4164 4920 493
Size: 711 2960 756 2702
Fragments arranged by size:
2960 2702 756 711
BsaBI GATnn'nnATC
Cuts at: 2660 7123 2660
Size: 4463 2666
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3101 4936 5318 5953
Size: 53 83 186 936 1568 1835 382 635
Cuts at: 5953 275
Size: 1451
Fragments arranged by size:
1835 1568 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2556 3024 3159 3257 4071 5562 5960 6234 6381
Size: 468 135 98 814 1491 398 274 147
Cuts at: 6381 6792 2556
Size: 411 2893
Fragments arranged by size:
2893 1491 814 468 411 398 274 147
135 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 2738 2768 1178
Size: 30 165 30 945 30 360 30 5539
Fragments arranged by size:
5539 945 360 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2503 2759 3023 3944 3962 4253 1186
Size: 1317 256 264 921 18 291 4062
Fragments arranged by size:
4062 1317 921 291 264 256 18
BseYI C'CCAG_C
Cuts at: 2757 2761 2862 3544 4012 4207 6332 6828 2757
Size: 4 101 682 468 195 2125 496 3058
Fragments arranged by size:
3058 2125 682 496 468 195 101 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2881 3319 3821 1163
Size: 573 1145 438 502 4471
Fragments arranged by size:
4471 1145 573 502 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2647 4386 4579 5340 6368
Size: 275 186 21 625 1739 193 761 1028
Cuts at: 6368 1540
Size: 2301
Fragments arranged by size:
2301 1739 1028 761 625 275 193 186
21
BsiHKAI G_wGCw'C
Cuts at: 2469 4218 5190 5275 6346 2469
Size: 1749 972 85 1071 3252
Fragments arranged by size:
3252 1749 1071 972 85
BsmI GAATG_Cn'
Cuts at: 4640 4733 4640
Size: 93 7036
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2469 2876 3039 3281 3526 3652 3690
Size: 399 679 407 163 242 245 126 38
Cuts at: 3690 4218 4448 5190 5275 6346 1391
Size: 528 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 528 407 399 245
242 230 163 126 85 38
BspEI T'CCGG_A
Cuts at: 3159 4071 6792 3159
Size: 912 2721 3496
Fragments arranged by size:
3496 2721 912
BspHI T'CATG_A
Cuts at: 5018 6748 5018
Size: 1730 5399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3943 3990 4011 4484 844
Size: 3099 47 21 473 3489
Fragments arranged by size:
3489 3099 473 47 21
BsrBI CCG'CTC
Cuts at: 1394 1823 4300 5016 1394
Size: 429 2477 716 3507
Fragments arranged by size:
3507 2477 716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2264 5624 5798 65
Size: 1183 1016 3360 174 1396
Fragments arranged by size:
3360 1396 1183 1016 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 2915 3564 3846 4381 5773 5960
Size: 110 746 359 649 282 535 1392 187
Cuts at: 5960 1700
Size: 2869
Fragments arranged by size:
2869 1392 746 649 535 359 282 187
110
BsrGI T'GTAC_A
Cuts at: 96 2850 96
Size: 2754 4375
BssHII G'CGCG_C
Cuts at: 1925 4419 1925
Size: 2494 4635
BssSI C'ACGA_G
Cuts at: 2692 3761 3815 4190 5189 6201 2692
Size: 1069 54 375 999 1012 3620
Fragments arranged by size:
3620 1069 1012 999 375 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3285 794
Size: 2491 4638
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7129
BstXI CCAn_nnnn'nTGG
Cuts at: 3569 4030 3569
Size: 461 6668
BstYI r'GATC_y
Cuts at: 1972 2655 3114 4459 4826 5211 5228 6680 6766
Size: 683 459 1345 367 385 17 1452 86
Cuts at: 6766 6778 7124 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1345 683 459 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4083 4083
Size: 7129
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2337 2346 2520 3121 3597
Size: 552 939 12 321 9 174 601 476
Cuts at: 3597 3615 3724 513
Size: 18 109 3918
Fragments arranged by size:
3918 939 601 552 476 321 174 109
18 12 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6390
Fragments arranged by size:
6390 683 56
BtsI GCAGTG_nn'
Cuts at: 2211 2546 2849 2893 3838 4183 4645 5439 5459
Size: 335 303 44 945 345 462 794 20
Cuts at: 5459 2211
Size: 3881
Fragments arranged by size:
3881 945 794 462 345 335 303 44
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DraIII CAC_nnn'GTG
Cuts at: 2473 3274 4048 2473
Size: 801 774 5554
Fragments arranged by size:
5554 801 774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3887 6136 809
Size: 507 2571 2249 1802
Fragments arranged by size:
2571 2249 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2110 2340
Size: 54 1425 50 275 18 189 91 230
Cuts at: 2340 2517 2623 2644 3124 3229 3486 3510 3612
Size: 177 106 21 480 105 257 24 102
Cuts at: 3612 4020 4383 4576 5465 7094 8
Size: 408 363 193 889 1629 43
Fragments arranged by size:
1629 1425 889 480 408 363 275 257
230 193 189 177 106 105 102 91
54 50 43 24 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 2644 4383 4576 1537
Size: 275 207 625 1739 193 4090
Fragments arranged by size:
4090 1739 625 275 207 193
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2513 3360 5059 1353
Size: 773 387 847 1699 3423
Fragments arranged by size:
3423 1699 847 773 387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2100 3932 5716 6090 6236 387
Size: 1064 138 511 1832 1784 374 146 1280
Fragments arranged by size:
1832 1784 1280 1064 511 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2596 2660 3232 3320 3638 3646
Size: 393 575 389 64 572 88 318 8
Cuts at: 3646 3725 4313 5192 5789 6576 6844 1239
Size: 79 588 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 588 575 572 393
389 318 268 88 79 64 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 3410 3899 2315
Size: 1095 489 5545
Fragments arranged by size:
5545 1095 489
EcoO109I rG'GnC_Cy
Cuts at: 2671 2873 3523 4078 4141 4231 4279 4323 2671
Size: 202 650 555 63 90 48 44 5477
Fragments arranged by size:
5477 650 555 202 90 63 48 44
EcoRI G'AATT_C
Cuts at: 4438 4816 4438
Size: 378 6751
EcoRV GAT'ATC
Cuts at: 3136 3136
Size: 7129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3325 3730 3325
Size: 405 6724
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 2595 2619 3433 4013 4274 6276 1678
Size: 917 24 814 580 261 2002 2531
Fragments arranged by size:
2531 2002 917 814 580 261 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 2348 2380 2738
Size: 32 135 32 670 32 74 32 358
Cuts at: 2738 2770 5770 5802 5844 5876 1373
Size: 32 3000 32 42 32 2626
Fragments arranged by size:
3000 2626 670 358 135 74 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 4473 4719 669
Size: 988 281 208 2327 246 3079
Fragments arranged by size:
3079 2327 988 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7129
HpaI GTT'AAC
Cuts at: 4719 4719
Size: 7129
KpnI G_GTAC'C
Cuts at: 4003 4454 4003
Size: 451 6678
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4141 6243 6427 1305
Size: 2836 2102 184 2007
Fragments arranged by size:
2836 2102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2112 2342 3231 3488 3614
Size: 54 1425 343 280 230 889 257 126
Cuts at: 3614 4022 10
Size: 408 3117
Fragments arranged by size:
3117 1425 889 408 343 280 257 230
126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 3567 5087 5446 5605 518
Size: 648 1371 1030 1520 359 159 2042
Fragments arranged by size:
2042 1520 1371 1030 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2098 2761 2798 3183 3541 3626 3666
Size: 412 80 663 37 385 358 85 40
Cuts at: 3666 3736 3747 4312 4545 5222 6370 6615 1606
Size: 70 11 565 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 565 412 385 358
245 233 85 80 70 40 37 11
NaeI GCC'GGC
Cuts at: 2917 3848 4383 2917
Size: 931 535 5663
Fragments arranged by size:
5663 931 535
NcoI C'CATG_G
Cuts at: 513 1065 2004 2346 3597 513
Size: 552 939 342 1251 4045
Fragments arranged by size:
4045 1251 939 552 342
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NgoMIV G'CCGG_C
Cuts at: 2915 3846 4381 2915
Size: 931 535 5663
Fragments arranged by size:
5663 931 535
NheI G'CTAG_C
Cuts at: 1052 2374 1052
Size: 1322 5807
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 4487 6032 1132
Size: 97 3258 1545 2229
Fragments arranged by size:
3258 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2876 4401 1348
Size: 1528 1525 4076
Fragments arranged by size:
4076 1528 1525
PciI A'CATG_T
Cuts at: 6028 6028
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3519 3544 3610 4401 1108
Size: 2411 25 66 791 3836
Fragments arranged by size:
3836 2411 791 66 25
PfoI T'CCnGG_A
Cuts at: 3654 3654
Size: 7129
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PmlI CAC'GTG
Cuts at: 4164 4164
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5198 5230 6711 6743 4160
Size: 32 1006 32 1481 32 4546
Fragments arranged by size:
4546 1481 1006 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 4078 4323 2671
Size: 1407 245 5477
Fragments arranged by size:
5477 1407 245
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PspOMI G'GGCC_C
Cuts at: 2872 3035 3522 2872
Size: 163 487 6479
Fragments arranged by size:
6479 487 163
PstI C_TGCA'G
Cuts at: 830 2553 2985 3099 3414 3845 4481 830
Size: 1723 432 114 315 431 636 3478
Fragments arranged by size:
3478 1723 636 432 431 315 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 2098 2761 2798 3183 3541 3626 3666 3736 2098
Size: 663 37 385 358 85 40 70 5491
Fragments arranged by size:
5491 663 385 358 85 70 40 37
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 7129
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SanDI GG'GwC_CC
Cuts at: 4078 4078
Size: 7129
SapI GCTCTTCn'nnn_
Cuts at: 2513 2513
Size: 7129
SbfI CC_TGCA'GG
Cuts at: 3414 4481 3414
Size: 1067 6062
ScaI AGT'ACT
Cuts at: 5377 7089 5377
Size: 1712 5417
SexAI A'CCwGG_T
Cuts at: 3603 3603
Size: 7129
SfcI C'TryA_G
Cuts at: 826 1045 2549 2587 2981 3001 3095 3410 3841
Size: 219 1504 38 394 20 94 315 431
Cuts at: 3841 4477 5612 6293 6484 826
Size: 636 1135 681 191 1471
Fragments arranged by size:
1504 1471 1135 681 636 431 394 315
219 191 94 38 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 2168 2339 2348 2168
Size: 171 9 6949
Fragments arranged by size:
6949 171 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 2915 3564 1700
Size: 1215 649 5265
Fragments arranged by size:
5265 1215 649
SmaI CCC'GGG
Cuts at: 1872 4456 1872
Size: 2584 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3671 4219 4274 5233 6134 6396
Size: 197 926 1728 548 55 959 901 262
Cuts at: 6396 820
Size: 1553
Fragments arranged by size:
1728 1553 959 926 901 548 262 197
55
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7129
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 5053 5
Size: 47 5001 2081
Fragments arranged by size:
5001 2081 47
StuI AGG'CCT
Cuts at: 1517 2781 4088 1517
Size: 1264 1307 4558
Fragments arranged by size:
4558 1307 1264
StyI C'CwwG_G
Cuts at: 513 1065 2004 2346 2806 3189 3597 4568 513
Size: 552 939 342 460 383 408 971 3074
Fragments arranged by size:
3074 971 939 552 460 408 383 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2753 3536 3926 4199 5156 5173 5326
Size: 1255 1173 783 390 273 957 17 153
Cuts at: 5326 5511 5956 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1173 957 783 445 390 273
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 2850 5375
Size: 274 80 33 51 1443 807 66 2525
Cuts at: 5375 7087 96
Size: 1712 138
Fragments arranged by size:
2525 1712 1443 807 274 138 80 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2427 2459 4340 4372 7089 7121 2427
Size: 32 1881 32 2717 32 2435
Fragments arranged by size:
2717 2435 1881 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3824 1316
Size: 2508 4621
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 3866 2050
Size: 1816 5313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7129
XmaI C'CCGG_G
Cuts at: 1870 4454 1870
Size: 2584 4545
XmnI GAAnn'nnTTC
Cuts at: 2990 5258 2990
Size: 2268 4861
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3102 4937 276
Size: 53 83 186 936 1568 1835 2468
Fragments arranged by size:
2468 1835 1568 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacI SfoI SrfI SwaI