Restriction map for
pFN21AB3881
(Circular) (Six-base) MAPSORT of: pFN21AB3881.seq Check: 4463 from: 1 to: 5812
pFN21AB3881 5812 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 3155 1937
Size: 718 500 4594
Fragments arranged by size:
4594 718 500
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 2630 2738 3875 5259 5527 1632
Size: 698 300 108 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 698 300 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 4711 5688 1203
Size: 3508 977 1327
Fragments arranged by size:
3508 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 2321 2759 1166
Size: 1155 438 4219
Fragments arranged by size:
4219 1155 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2033 2894 1184
Size: 849 861 4102
Fragments arranged by size:
4102 861 849
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaLI G'TGCA_C
Cuts at: 2105 2462 2909 3869 5025 2105
Size: 357 447 960 1156 2892
Fragments arranged by size:
2892 1156 960 447 357
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 1870
Size: 73 1194 4545
Fragments arranged by size:
4545 1194 73
BamHI G'GATC_C
Cuts at: 2619 2646 3142 3509 2619
Size: 27 496 367 4922
Fragments arranged by size:
4922 496 367 27
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3133 4588 618
Size: 325 217 627 1346 1455 1842
Fragments arranged by size:
1842 1455 1346 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2987 3131 1391
Size: 1596 144 4072
Fragments arranged by size:
4072 1596 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4841
BbvCI CC'TCA_GC
Cuts at: 2238 2238
Size: 5812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2448 2482 4003 4037 1903
Size: 34 511 34 1521 34 3678
Fragments arranged by size:
3678 1521 511 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3229 3229
Size: 5812
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2466 2913 3873 5029 1790
Size: 319 357 447 960 1156 2573
Fragments arranged by size:
2573 1156 960 447 357 319
BmgBI CAC'GTC
Cuts at: 1318 2896 2901 2908 1318
Size: 1578 5 7 4222
Fragments arranged by size:
4222 1578 7 5
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3543 4501
Size: 416 53 83 399 24 1169 957 958
Cuts at: 4501 442
Size: 1753
Fragments arranged by size:
1753 1169 958 957 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4472 1239
Size: 3233 2579
Bpu10I CC'TnA_GC
Cuts at: 2238 2238
Size: 5812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 3937 4802 5100 2873
Size: 1064 865 298 3585
Fragments arranged by size:
3585 1064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2077 3603 493
Size: 711 873 1526 2702
Fragments arranged by size:
2702 1526 873 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3018 3619 4001 4636
Size: 53 83 186 936 1485 601 382 635
Cuts at: 4636 275
Size: 1451
Fragments arranged by size:
1485 1451 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2220 2601 4245 4643 4917 5064 5475 2220
Size: 381 1644 398 274 147 411 2557
Fragments arranged by size:
2557 1644 411 398 381 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 1178
Size: 30 165 30 1065 30 4492
Fragments arranged by size:
4492 1065 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2306 2642 1186
Size: 1069 51 336 4356
Fragments arranged by size:
4356 1069 336 51
BseYI C'CCAG_C
Cuts at: 5015 5511 5015
Size: 496 5316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2109 2466 2913 3873 3958 5029 2012
Size: 97 357 447 960 85 1071 2795
Fragments arranged by size:
2795 1071 960 447 357 97 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2109 2466 2913 2987 3131 3873
Size: 399 222 97 357 447 74 144 742
Cuts at: 3873 3958 5029 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 357 222 144
97 85 74
BspEI T'CCGG_A
Cuts at: 2601 5475 2601
Size: 2874 2938
BspHI T'CATG_A
Cuts at: 2526 3701 5431 2526
Size: 1175 1730 2907
Fragments arranged by size:
2907 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3167 844
Size: 2323 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2427 2617 3699 1394
Size: 429 604 190 1082 3507
Fragments arranged by size:
3507 1082 604 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 2534 2833 4307 4481 65
Size: 1183 1286 299 1474 174 1396
Fragments arranged by size:
1474 1396 1286 1183 299 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 2211 96
Size: 2115 3697
BssHII G'CGCG_C
Cuts at: 1925 2727 2928 1925
Size: 802 201 4809
Fragments arranged by size:
4809 802 201
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2503 3076 3100 794
Size: 1709 573 24 3506
Fragments arranged by size:
3506 1709 573 24
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5812
BstYI r'GATC_y
Cuts at: 1972 2598 2619 2646 3142 3509 3894 3911 5363
Size: 626 21 27 496 367 385 17 1452
Cuts at: 5363 5449 5461 5807 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 626 496 385 367 346 86
27 21 17 12
BstZ17I GTA'TAC
Cuts at: 2656 2656
Size: 5812
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2551 487
Size: 683 56 1325 3748
Fragments arranged by size:
3748 1325 683 56
BtsI GCAGTG_nn'
Cuts at: 2090 2464 2932 3328 4122 4142 2090
Size: 374 468 396 794 20 3760
Fragments arranged by size:
3760 794 468 396 374 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DraIII CAC_nnn'GTG
Cuts at: 2281 2364 2281
Size: 83 5729
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 4819 809
Size: 507 1269 2234 1802
Fragments arranged by size:
2234 1802 1269 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2507 3259 4148
Size: 54 1425 50 275 18 677 752 889
Cuts at: 4148 5777 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 752 677 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 3742 1353
Size: 848 1541 3423
Fragments arranged by size:
3423 1541 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2630 2738 3875 4472 5259 5527
Size: 393 698 300 108 1137 597 787 268
Cuts at: 5527 1239
Size: 1524
Fragments arranged by size:
1524 1137 787 698 597 393 300 268
108
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 5812
FspI TGC'GCA
Cuts at: 2855 4318 2855
Size: 1463 4349
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373
Size: 32 135 32 2881 32 42 32 2626
Fragments arranged by size:
2881 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 2551 3156 3402 669
Size: 988 281 415 198 605 246 3079
Fragments arranged by size:
3079 988 605 415 281 246 198
HindIII A'AGCT_T
Cuts at: 748 3032 748
Size: 2284 3528
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2321 2759 2876 3770 4129 4288
Size: 648 978 177 438 117 894 359 159
Cuts at: 4288 518
Size: 2042
Fragments arranged by size:
2042 978 894 648 438 359 177 159
117
MspA1I CmG'CkG
Cuts at: 1606 2779 3004 3228 3905 5053 5298 1606
Size: 1173 225 224 677 1148 245 2120
Fragments arranged by size:
2120 1173 1148 677 245 225 224
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5260
NdeI CA'TA_TG
Cuts at: 387 2877 387
Size: 2490 3322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2863 3070 3170 4715 1132
Size: 97 1634 207 100 1545 2229
Fragments arranged by size:
2229 1634 1545 207 100 97
PasI CC'CwG_GG
Cuts at: 1348 2802 1348
Size: 1454 4358
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PfoI T'CCnGG_A
Cuts at: 2622 2622
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PstI C_TGCA'G
Cuts at: 830 3084 3164 830
Size: 2254 80 3478
Fragments arranged by size:
3478 2254 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 2411 4060 5772 2411
Size: 1649 1712 2451
Fragments arranged by size:
2451 1712 1649
SexAI A'CCwGG_T
Cuts at: 2100 3108 2100
Size: 1008 4804
SfcI C'TryA_G
Cuts at: 826 1045 3080 3160 4295 4976 5167 826
Size: 219 2035 80 1135 681 191 1471
Fragments arranged by size:
2035 1471 1135 681 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 3916 4817 5079 820
Size: 197 926 945 1028 901 262 1553
Fragments arranged by size:
1553 1028 945 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3070 3170 1132
Size: 1938 100 3774
Fragments arranged by size:
3774 1938 100
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 2016 2166 1517
Size: 499 150 5163
Fragments arranged by size:
5163 499 150
StyI C'CwwG_G
Cuts at: 513 1065 2769 3251 513
Size: 552 1704 482 3074
Fragments arranged by size:
3074 1704 552 482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3839 3856 4009 4194 4639 325
Size: 1255 2259 17 153 185 445 1498
Fragments arranged by size:
2259 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 2409 4058
Size: 274 80 33 51 1443 234 198 1649
Cuts at: 4058 5770 96
Size: 1712 138
Fragments arranged by size:
1712 1649 1443 274 234 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2885 2917 5772 5804 2885
Size: 32 2855 32 2893
Fragments arranged by size:
2893 2855 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2348 1316
Size: 1032 4780
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI