Restriction map for
pFN21AB3867
(Circular) (Six-base) MAPSORT of: pFN21AB3867.seq Check: 8304 from: 1 to: 5767
pFN21AB3867 5767 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2610 2661 3577 278
Size: 53 83 186 936 1074 51 916 2468
Fragments arranged by size:
2468 1074 936 916 186 83 53 51
AccI GT'mk_AC
Cuts at: 1937 2223 3110 1937
Size: 286 887 4594
Fragments arranged by size:
4594 887 286
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2578 3830 5214 5482 1632
Size: 946 1252 1384 268 1917
Fragments arranged by size:
1917 1384 1252 946 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 2430 4598 2430
Size: 2168 3599
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2150 1166
Size: 984 4783
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2245 3510 5082 2245
Size: 1265 1572 2930
Fragments arranged by size:
2930 1572 1265
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2120 2436 3092 1870
Size: 73 99 78 316 656 4545
Fragments arranged by size:
4545 656 316 99 78 73
BamHI G'GATC_C
Cuts at: 2640 3097 3464 2640
Size: 457 367 4943
Fragments arranged by size:
4943 457 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2361 3086 1391
Size: 970 725 4072
Fragments arranged by size:
4072 970 725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2355 2721 2904 928
Size: 971 303 153 366 183 3791
Fragments arranged by size:
3791 971 366 303 183 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2716 2750 3958 3992 1903
Size: 34 779 34 1208 34 3678
Fragments arranged by size:
3678 1208 779 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2821 5762 2821
Size: 2941 2826
BlpI GC'TnA_GC
Cuts at: 2687 3184 2687
Size: 497 5270
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 2104 2200 1318
Size: 786 96 4885
Fragments arranged by size:
4885 786 96
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2380 2057
Size: 323 5444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 4427 1239
Size: 909 2279 2579
Fragments arranged by size:
2579 2279 909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 2165 5761 2165
Size: 3596 2171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 2607 2658 3015
Size: 53 83 186 936 780 294 51 357
Cuts at: 3015 3574 3956 4591 275
Size: 559 382 635 1451
Fragments arranged by size:
1451 936 780 635 559 382 357 294
186 83 53 51
BsaWI w'CCGG_w
Cuts at: 2189 2430 4200 4598 4872 5019 5430 2189
Size: 241 1770 398 274 147 411 2526
Fragments arranged by size:
2526 1770 411 398 274 241 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 1178
Size: 30 165 30 781 30 4731
Fragments arranged by size:
4731 781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 1186
Size: 856 4911
BseYI C'CCAG_C
Cuts at: 4970 5466 4970
Size: 496 5271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2709 3828 3913 4984 2709
Size: 1119 85 1071 3492
Fragments arranged by size:
3492 1119 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2709 3086 3828 3913 4984 1391
Size: 399 571 348 377 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 399 377 348 85
BspEI T'CCGG_A
Cuts at: 2189 5430 2189
Size: 3241 2526
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 2943 3654 1394
Size: 429 224 896 711 3507
Fragments arranged by size:
3507 896 711 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 2644 3033 4262 4436 65
Size: 1183 1396 389 1229 174 1396
Fragments arranged by size:
1396 1396 1229 1183 389 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 4411 4598 1700
Size: 110 620 1981 187 2869
Fragments arranged by size:
2869 1981 620 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2738 3827 4839 2738
Size: 1089 1012 3666
Fragments arranged by size:
3666 1089 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2180
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2640 2821 3097 3464 3849 3866 5318 5404
Size: 668 181 276 367 385 17 1452 86
Cuts at: 5404 5416 5762 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 668 385 367 346 276 181
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4015
Fragments arranged by size:
4015 939 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 2787 1554
Size: 1233 4534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2664 3214 4103
Size: 54 1425 50 275 18 834 550 889
Cuts at: 4103 5732 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 834 550 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 2810 3697 1353
Size: 1406 51 887 3423
Fragments arranged by size:
3423 1406 887 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2578 3830 4427 5214 5482 1239
Size: 393 516 430 1252 597 787 268 1524
Fragments arranged by size:
1524 1252 787 597 516 430 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 5767
EcoO109I rG'GnC_Cy
Cuts at: 2882 2882
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2094 4914 1678
Size: 416 2820 2531
Fragments arranged by size:
2820 2531 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 4408 4440 4482
Size: 32 135 32 599 32 2205 32 42
Cuts at: 4482 4514 1373
Size: 32 2626
Fragments arranged by size:
2626 2205 599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 2590 4881 5065 1305
Size: 1016 269 2291 184 2007
Fragments arranged by size:
2291 2007 1016 269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2178 3002 3725 4084 4243 518
Size: 648 984 28 824 723 359 159 2042
Fragments arranged by size:
2042 984 824 723 648 359 159 28
MspA1I CmG'CkG
Cuts at: 1606 2691 3183 3860 5008 5253 1606
Size: 1085 492 677 1148 245 2120
Fragments arranged by size:
2120 1148 1085 677 492 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4015
Fragments arranged by size:
4015 939 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2737 3125 4670 1132
Size: 97 1508 388 1545 2229
Fragments arranged by size:
2229 1545 1508 388 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 1108
Size: 1137 4630
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspXI vC'TCGA_Gb
Cuts at: 2042 2042
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2371 3119 830
Size: 1541 748 3478
Fragments arranged by size:
3478 1541 748
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SapI GCTCTTCn'nnn_
Cuts at: 2759 2759
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 5767
SfcI C'TryA_G
Cuts at: 826 1045 2224 2367 3115 4250 4931 5122 826
Size: 219 1179 143 748 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 748 681 219 191 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2438 3094 1872
Size: 566 656 4545
Fragments arranged by size:
4545 656 566
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 3871 4772 5034 820
Size: 197 926 99 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197 99
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 2737 3125 1132
Size: 1605 388 3774
Fragments arranged by size:
3774 1605 388
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2265 3206 513
Size: 552 939 261 941 3074
Fragments arranged by size:
3074 941 939 552 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2962 3794 3811 3964 4149 4594 325
Size: 1255 1382 832 17 153 185 445 1498
Fragments arranged by size:
1498 1382 1255 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2012 2208 4013
Size: 274 80 33 51 1443 35 196 1805
Cuts at: 4013 5725 96
Size: 1712 138
Fragments arranged by size:
1805 1712 1443 274 196 138 80 51
35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 5727 5759 2184
Size: 32 3511 32 2192
Fragments arranged by size:
3511 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3017 1316
Size: 1701 4066
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2470 2272
Size: 198 5569
XhoI C'TCGA_G
Cuts at: 1943 2042 1943
Size: 99 5668
XmaI C'CCGG_G
Cuts at: 1870 2436 3092 1870
Size: 566 656 4545
Fragments arranged by size:
4545 656 566
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2608 2659 3575 276
Size: 53 83 186 936 1074 51 916 2468
Fragments arranged by size:
2468 1074 936 916 186 83 53 51
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI