Restriction map for
pFN21AB3865
(Circular) (Six-base) MAPSORT of: pFN21AB3865.seq Check: 7433 from: 1 to: 5707
pFN21AB3865 5707 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2442 2442
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2577 3050 1937
Size: 640 473 4594
Fragments arranged by size:
4594 640 473
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3770 5154 5422 1632
Size: 2138 1384 268 1917
Fragments arranged by size:
2138 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 2217 4606 5583 1203
Size: 1014 2389 977 1327
Fragments arranged by size:
2389 1327 1014 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2929 1184
Size: 1745 3962
AlwNI CAG_nnn'CTG
Cuts at: 3450 5022 3450
Size: 1572 4135
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 2153 2501 3028 4483
Size: 325 217 627 289 77 348 527 1455
Cuts at: 4483 618
Size: 1842
Fragments arranged by size:
1842 1455 627 527 348 325 289 217
77
BanII G_rGCy'C
Cuts at: 1391 2616 2901 3026 1391
Size: 1225 285 125 4072
Fragments arranged by size:
4072 1225 285 125
BbeI G_GCGC'C
Cuts at: 2157 2157
Size: 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2735 928
Size: 971 836 3900
Fragments arranged by size:
3900 971 836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3898 3932 1903
Size: 34 50 34 1877 34 3678
Fragments arranged by size:
3678 1877 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2568 4318 136
Size: 107 122 71 631 605 896 1750 1525
Fragments arranged by size:
1750 1525 896 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2343 2504 3768 4924 1790
Size: 553 161 1264 1156 2573
Fragments arranged by size:
2573 1264 1156 553 161
BmgBI CAC'GTC
Cuts at: 1318 2178 1318
Size: 860 4847
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2542 2630 2915 4367 1239
Size: 1303 88 285 1452 2579
Fragments arranged by size:
2579 1452 1303 285 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2534 4370 882
Size: 617 1035 1836 2219
Fragments arranged by size:
2219 1836 1035 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 2266 2338 2440 3498 493
Size: 711 1014 48 72 102 1058 2702
Fragments arranged by size:
2702 1058 1014 711 102 72 48
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 2273 2384 2447
Size: 53 83 186 936 621 119 111 63
Cuts at: 2447 2475 3514 3896 4531 275
Size: 28 1039 382 635 1451
Fragments arranged by size:
1451 1039 936 635 621 382 186 119
111 83 63 53 28
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 4910 5406 4910
Size: 496 5211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2158 2315 2318 2420 2899 1163
Size: 573 422 157 3 102 479 3971
Fragments arranged by size:
3971 573 479 422 157 102 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3157 3918 4946 1540
Size: 275 186 33 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2087 2616 3768 3853 4924 2087
Size: 529 1152 85 1071 2870
Fragments arranged by size:
2870 1152 1071 529 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2008 2438 2466 2008
Size: 430 28 5249
Fragments arranged by size:
5249 430 28
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2343 2504 2616 2901 3026 3768
Size: 399 297 256 161 112 285 125 742
Cuts at: 3768 3853 4924 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 285 256 161
125 112 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 2796 3062 844
Size: 1598 354 266 3489
Fragments arranged by size:
3489 1598 354 266
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2414 2748 4351 4538 1700
Size: 110 604 334 1603 187 2869
Fragments arranged by size:
2869 1603 604 334 187 110
BsrGI T'GTAC_A
Cuts at: 96 2220 96
Size: 2124 3583
BssHII G'CGCG_C
Cuts at: 1925 2396 1925
Size: 471 5236
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 2619 4511 2619
Size: 1892 3815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2926 2266
Size: 660 5047
BstYI r'GATC_y
Cuts at: 1972 2922 3037 3404 3789 3806 5258 5344 5356
Size: 950 115 367 385 17 1452 86 12
Cuts at: 5356 5702 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 950 385 367 346 115 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2237 2495 2693 513
Size: 552 1172 258 198 3527
Fragments arranged by size:
3527 1172 552 258 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2131 3223 4017 4037 2131
Size: 1092 794 20 3801
Fragments arranged by size:
3801 1092 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2269 2269
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2750 3154
Size: 54 1425 50 275 18 489 431 404
Cuts at: 3154 4043 5672 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 489 431 404 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2616 2621 3637 1353
Size: 653 610 5 1016 3423
Fragments arranged by size:
3423 1016 653 610 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2614 2614
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2542 2630 2915 3770 4367 5154 5422
Size: 393 910 88 285 855 597 787 268
Cuts at: 5422 1239
Size: 1524
Fragments arranged by size:
1524 910 855 787 597 393 285 268
88
EcoO109I rG'GnC_Cy
Cuts at: 2689 2689
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 5707
FspI TGC'GCA
Cuts at: 2207 2630 4213 2207
Size: 423 1583 3701
Fragments arranged by size:
3701 1583 423
FspAI rTGC'GCAy
Cuts at: 2207 2207
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2157 4854 1678
Size: 479 2697 2531
Fragments arranged by size:
2697 2531 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 2766 2798 2862
Size: 32 135 32 435 32 727 32 64
Cuts at: 2862 2894 4348 4380 4422 4454 1373
Size: 32 1454 32 42 32 2626
Fragments arranged by size:
2626 1454 727 435 135 64 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2785 3051 3297 669
Size: 988 281 847 266 246 3079
Fragments arranged by size:
3079 988 847 281 266 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KasI G'GCGC_C
Cuts at: 2153 2153
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 4821 5005 1305
Size: 963 2553 184 2007
Fragments arranged by size:
2553 2007 963 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 10
Size: 54 1425 343 489 3396
Fragments arranged by size:
3396 1425 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2264 3665 4024 4183 518
Size: 648 949 149 1401 359 159 2042
Fragments arranged by size:
2042 1401 949 648 359 159 149
MspA1I CmG'CkG
Cuts at: 1606 2389 2497 3123 3800 4948 5193 1606
Size: 783 108 626 677 1148 245 2120
Fragments arranged by size:
2120 1148 783 677 626 245 108
NarI GG'CG_CC
Cuts at: 2154 2154
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2237 513
Size: 552 1172 3983
Fragments arranged by size:
3983 1172 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2163 1348
Size: 815 4892
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 1108
Size: 1248 4459
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PmlI CAC'GTG
Cuts at: 2266 2338 2440 2266
Size: 72 102 5533
Fragments arranged by size:
5533 102 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2805 3059 830
Size: 1975 254 3478
Fragments arranged by size:
3478 1975 254
PvuI CG_AT'CG
Cuts at: 2001 2034 2001
Size: 33 5674
SacI G_AGCT'C
Cuts at: 2616 2616
Size: 5707
SacII CC_GC'GG
Cuts at: 2498 2498
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SapI GCTCTTCn'nnn_
Cuts at: 2621 2621
Size: 5707
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 2171 3955 5667 2171
Size: 1784 1712 2211
Fragments arranged by size:
2211 1784 1712
SfcI C'TryA_G
Cuts at: 826 1045 2281 2602 2801 3055 4190 4871 5062
Size: 219 1236 321 199 254 1135 681 191
Cuts at: 5062 826
Size: 1471
Fragments arranged by size:
1471 1236 1135 681 321 254 219 199
191
SfoI GGC'GCC
Cuts at: 2155 2155
Size: 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 2816 1517
Size: 1299 4408
StyI C'CwwG_G
Cuts at: 513 1065 2237 2901 3146 513
Size: 552 1172 664 245 3074
Fragments arranged by size:
3074 1172 664 552 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2660 3734 3751 3904 4089 4534 325
Size: 1255 1080 1074 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1080 1074 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 2220 3953
Size: 274 80 33 51 1443 192 51 1733
Cuts at: 3953 5665 96
Size: 1712 138
Fragments arranged by size:
1733 1712 1443 274 192 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2263
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2981 3836 2981
Size: 855 4852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI
NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI
SwaI