Restriction map for
pFN21AB3864
(Circular) (Six-base) MAPSORT of: pFN21AB3864.seq Check: 4408 from: 1 to: 8131
pFN21AB3864 8131 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2824 5219 2824
Size: 2395 5736
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5941 278
Size: 53 83 186 936 4405 2468
Fragments arranged by size:
4405 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5474 1937
Size: 3537 4594
Acc65I G'GTAC_C
Cuts at: 3810 5452 3810
Size: 1642 6489
AclI AA'CG_TT
Cuts at: 1563 6258 6631 1563
Size: 4695 373 3063
Fragments arranged by size:
4695 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3023 3263 3285 3765 6194 7578 7846 1632
Size: 1391 240 22 480 2429 1384 268 1917
Fragments arranged by size:
2429 1917 1391 1384 480 268 240 22
AfeI AGC'GCT
Cuts at: 4637 4637
Size: 8131
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7934
AflIII A'CryG_T
Cuts at: 1203 7030 8007 1203
Size: 5827 977 1327
Fragments arranged by size:
5827 1327 977
AgeI A'CCGG_T
Cuts at: 6962 6962
Size: 8131
AhdI GACnn_n'nnGTC
Cuts at: 6860 6860
Size: 8131
AleI CACnn'nnGTG
Cuts at: 1166 3284 3977 4988 1166
Size: 2118 693 1011 4309
Fragments arranged by size:
4309 2118 1011 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4379 5252 1184
Size: 3195 873 4063
Fragments arranged by size:
4063 3195 873
AlwNI CAG_nnn'CTG
Cuts at: 2459 2866 4018 4093 4594 4703 5125 5356 5874
Size: 407 1152 75 501 109 422 231 518
Cuts at: 5874 7446 2459
Size: 1572 3144
Fragments arranged by size:
3144 1572 1152 518 501 422 407 231
109 75
ApaI G_GGCC'C
Cuts at: 2582 2716 3417 4318 4382 2582
Size: 134 701 901 64 6331
Fragments arranged by size:
6331 901 701 134 64
ApaLI G'TGCA_C
Cuts at: 6188 7344 6188
Size: 1156 6975
ApoI r'AATT_y
Cuts at: 1493 5440 5666 5818 7927 1493
Size: 3947 226 152 2109 1697
Fragments arranged by size:
3947 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6685 160
Size: 872 5653 1606
Fragments arranged by size:
5653 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8131
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2180 2709 2715 4390 4436 5456
Size: 73 87 150 529 6 1675 46 1020
Cuts at: 5456 1870
Size: 4545
Fragments arranged by size:
4545 1675 1020 529 150 87 73 46
6
BamHI G'GATC_C
Cuts at: 5046 5461 5828 5046
Size: 415 367 7349
Fragments arranged by size:
7349 415 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2062 2836 3127 3804
Size: 325 217 627 249 26 774 291 677
Cuts at: 3804 3810 5299 5452 6907 618
Size: 6 1489 153 1455 1842
Fragments arranged by size:
1842 1489 1455 774 677 627 325 291
249 217 153 26 6
BanII G_rGCy'C
Cuts at: 1391 2079 2469 2582 2600 2716 2759 3065 3417
Size: 688 390 113 18 116 43 306 352
Cuts at: 3417 3731 3773 4012 4087 4318 4382 4614 4799
Size: 314 42 239 75 231 64 232 185
Cuts at: 4799 4845 5450 1391
Size: 46 605 4072
Fragments arranged by size:
4072 688 605 390 352 314 306 239
232 231 185 116 113 75 64 46
43 42 18
BbeI G_GCGC'C
Cuts at: 2040 3808 2040
Size: 1768 6363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3938 4132 5192 928
Size: 971 2039 194 1060 3867
Fragments arranged by size:
3867 2039 1060 971 194
BbvCI CC'TCA_GC
Cuts at: 2630 3273 3893 2630
Size: 643 620 6868
Fragments arranged by size:
6868 643 620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6322 6356 1903
Size: 34 4385 34 3678
Fragments arranged by size:
4385 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5117 6023 7233 5117
Size: 906 1210 6015
Fragments arranged by size:
6015 1210 906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8131
BfrBI ATG'CAT
Cuts at: 5200 5200
Size: 8131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3503 4157 4609
Size: 107 122 71 631 605 1831 654 452
Cuts at: 4609 5010 5308 6742 136
Size: 401 298 1434 1525
Fragments arranged by size:
1831 1525 1434 654 631 605 452 401
298 122 107 71
BglII A'GATC_T
Cuts at: 4209 8126 4209
Size: 3917 4214
BlpI GC'TnA_GC
Cuts at: 4011 5548 4011
Size: 1537 6594
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2582 2716 2795 2806 2841 3417 4318
Size: 277 515 134 79 11 35 576 901
Cuts at: 4318 4363 4382 4669 6192 7348 1790
Size: 45 19 287 1523 1156 2573
Fragments arranged by size:
2573 1523 1156 901 576 515 287 277
134 79 45 35 19 11
BmgBI CAC'GTC
Cuts at: 1318 2095 5094 5190 1318
Size: 777 2999 96 4259
Fragments arranged by size:
4259 2999 777 96
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 3847 5862
Size: 416 53 83 399 24 2402 28 2015
Cuts at: 5862 6820 442
Size: 958 1753
Fragments arranged by size:
2402 2015 1753 958 416 399 83 53
28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 8131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2421 2593 2773 2927 3049 3272 3380 4115
Size: 1182 172 180 154 122 223 108 735
Cuts at: 4115 4340 4364 6791 1239
Size: 225 24 2427 2579
Fragments arranged by size:
2579 2427 1182 735 225 223 180 172
154 122 108 24
Bpu10I CC'TnA_GC
Cuts at: 2630 3273 3632 3893 5160 2630
Size: 643 359 261 1267 5601
Fragments arranged by size:
5601 1267 643 359 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3392 4775 6256 7121 7419 3392
Size: 1383 1481 865 298 4104
Fragments arranged by size:
4104 1481 1383 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4130 4241 6794 882
Size: 617 2631 111 2553 2219
Fragments arranged by size:
2631 2553 2219 617 111
BsaAI yAC'GTr
Cuts at: 493 1204 2990 5922 493
Size: 711 1786 2932 2702
Fragments arranged by size:
2932 2702 1786 711
BsaBI GATnn'nnATC
Cuts at: 8125 8125
Size: 8131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 3186 3805 3987
Size: 53 83 186 936 504 1149 619 182
Cuts at: 3987 5938 6320 6955 275
Size: 1951 382 635 1451
Fragments arranged by size:
1951 1451 1149 936 635 619 504 382
186 182 83 53
BsaWI w'CCGG_w
Cuts at: 4799 6564 6962 7236 7383 7794 4799
Size: 1765 398 274 147 411 5136
Fragments arranged by size:
5136 1765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2338 2368 2388 2418 2774
Size: 30 165 30 935 30 20 30 356
Cuts at: 2774 2804 2959 2989 1178
Size: 30 155 30 6320
Fragments arranged by size:
6320 935 356 165 155 30 30 30
30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2114 2117 2346 2402 2432 2441 4706
Size: 901 27 3 229 56 30 9 2265
Cuts at: 4706 4811 1186
Size: 105 4506
Fragments arranged by size:
4506 2265 901 229 105 56 30 27
9 3
BseYI C'CCAG_C
Cuts at: 2189 2601 2693 2702 2732 2866 2978 3104 3409
Size: 412 92 9 30 134 112 126 305
Cuts at: 3409 3416 3922 4003 4385 5118 5221 7334 7830
Size: 7 506 81 382 733 103 2113 496
Cuts at: 7830 2189
Size: 2490
Fragments arranged by size:
2490 2113 733 506 496 412 382 305
134 126 112 103 92 81 30 9
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3830 5402 1163
Size: 573 2094 1572 3892
Fragments arranged by size:
3892 2094 1572 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5581 6342 7370 1540
Size: 275 186 3580 761 1028 2301
Fragments arranged by size:
3580 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2624 3065 3873 3984 4012 4284 4995 5064 6192
Size: 441 808 111 28 272 711 69 1128
Cuts at: 6192 6277 7348 2624
Size: 85 1071 3407
Fragments arranged by size:
3407 1128 1071 808 711 441 272 111
85 69 28
BsmI GAATG_Cn'
Cuts at: 5642 5735 5642
Size: 93 8038
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2079 2469 2582 2600 2624 2716
Size: 399 277 12 390 113 18 24 92
Cuts at: 2716 2759 2795 2806 2841 3065 3417 3731 3773
Size: 43 36 11 35 224 352 314 42
Cuts at: 3773 3873 3984 4012 4087 4284 4318 4363 4382
Size: 100 111 28 75 197 34 45 19
Cuts at: 4382 4614 4669 4799 4845 4995 5064 5450 6192
Size: 232 55 130 46 150 69 386 742
Cuts at: 6192 6277 7348 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 386 352 314
277 232 224 197 150 130 113 111
100 92 85 75 69 55 46 45
43 42 36 35 34 28 24 19
18 12 11
BspEI T'CCGG_A
Cuts at: 7794 7794
Size: 8131
BspHI T'CATG_A
Cuts at: 3487 6020 7750 3487
Size: 2533 1730 3868
Fragments arranged by size:
3868 2533 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2784 2824 3603 3840 4824 5219 5486
Size: 1286 654 40 779 237 984 395 267
Cuts at: 5486 844
Size: 3489
Fragments arranged by size:
3489 1286 984 779 654 395 267 237
40
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 2257 4890 6018 1394
Size: 429 386 48 2633 1128 3507
Fragments arranged by size:
3507 2633 1128 429 386 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 3456 5311 6626 6800 65
Size: 1183 2208 1855 1315 174 1396
Fragments arranged by size:
2208 1855 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 2745 3513 3807 5143 6775 6962
Size: 110 416 519 768 294 1336 1632 187
Cuts at: 6962 1700
Size: 2869
Fragments arranged by size:
2869 1632 1336 768 519 416 294 187
110
BsrGI T'GTAC_A
Cuts at: 96 5079 96
Size: 4983 3148
BssHII G'CGCG_C
Cuts at: 1925 2264 1925
Size: 339 7792
BssSI C'ACGA_G
Cuts at: 3440 4639 5006 6191 7203 3440
Size: 1199 367 1185 1012 4368
Fragments arranged by size:
4368 1199 1185 1012 367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2619 2836 4288 794
Size: 1825 217 1452 4637
Fragments arranged by size:
4637 1825 1452 217
BstBI TT'CG_AA
Cuts at: 6935 6935
Size: 8131
BstEII G'GTnAC_C
Cuts at: 1184 3224 1184
Size: 2040 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 3212 5008 5026 2656
Size: 556 1796 18 5761
Fragments arranged by size:
5761 1796 556 18
BstYI r'GATC_y
Cuts at: 1972 3258 4209 4956 5046 5070 5461 5828 6213
Size: 1286 951 747 90 24 391 367 385
Cuts at: 6213 6230 7682 7768 7780 8126 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1286 951 747 391 385 367
346 90 86 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 2476 4308 2476
Size: 1832 6299
BtgI C'CryG_G
Cuts at: 513 1065 2004 3109 3135 3279 3606 4682 4881
Size: 552 939 1105 26 144 327 1076 199
Cuts at: 4881 5256 513
Size: 375 3388
Fragments arranged by size:
3388 1105 1076 939 552 375 327 199
144 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4918 4981 5215 487
Size: 683 56 3692 63 234 3403
Fragments arranged by size:
3692 3403 683 234 63 56
BtsI GCAGTG_nn'
Cuts at: 2806 4286 4363 4412 4669 5234 5647 6441 6461
Size: 1480 77 49 257 565 413 794 20
Cuts at: 6461 2806
Size: 4476
Fragments arranged by size:
4476 1480 794 565 413 257 77 49
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2800 2835 3016 3051 529
Size: 35 2236 35 181 35 5609
Fragments arranged by size:
5609 2236 181 35 35 35
DraI TTT'AAA
Cuts at: 5435 5435
Size: 8131
DraIII CAC_nnn'GTG
Cuts at: 2938 3683 2938
Size: 745 7386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7138 809
Size: 507 5822 1802
Fragments arranged by size:
5822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2763 3170
Size: 54 1425 50 275 18 177 756 407
Cuts at: 3170 3861 4108 4158 5395 5578 6467 8096 8
Size: 691 247 50 1237 183 889 1629 43
Fragments arranged by size:
1629 1425 1237 889 756 691 407 275
247 183 177 54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5578 1537
Size: 275 3766 4090
Fragments arranged by size:
4090 3766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2409 2418 2427 2888 3404 3878 3998 5336
Size: 1056 9 9 461 516 474 120 1338
Cuts at: 5336 6061 1353
Size: 725 3423
Fragments arranged by size:
3423 1338 1056 725 516 474 461 120
9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2867 5209 6718 7092 7238 387
Size: 1064 138 1278 2342 1509 374 146 1280
Fragments arranged by size:
2342 1509 1280 1278 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3063 4010 3063
Size: 947 7184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2421 2593 2773 2927 3023 3049 3263
Size: 393 789 172 180 154 96 26 214
Cuts at: 3263 3272 3285 3380 3765 4115 4340 4364 6194
Size: 9 13 95 385 350 225 24 1830
Cuts at: 6194 6791 7578 7846 1239
Size: 597 787 268 1524
Fragments arranged by size:
1830 1524 789 787 597 393 385 350
268 225 214 180 172 154 96 95
26 24 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2051
Size: 8131
EcoO109I rG'GnC_Cy
Cuts at: 2149 2176 2370 2481 3376 3413 4090 4314 4315
Size: 27 194 111 895 37 677 224 1
Cuts at: 4315 4326 4378 4379 4440 2149
Size: 11 52 1 61 5840
Fragments arranged by size:
5840 895 677 224 194 111 61 52
37 27 11 1 1
EcoRI G'AATT_C
Cuts at: 5440 5818 5440
Size: 378 7753
FspI TGC'GCA
Cuts at: 4466 6637 4466
Size: 2171 5960
HaeII r_GCGC'y
Cuts at: 1678 2040 3454 3808 4639 4760 5357 7278 1678
Size: 362 1414 354 831 121 597 1921 2531
Fragments arranged by size:
2531 1921 1414 831 597 362 354 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2959 2991 4622 4654 6772
Size: 32 135 32 1387 32 1631 32 2118
Cuts at: 6772 6804 6846 6878 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2118 1631 1387 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4914 5475 5721 669
Size: 988 281 2976 561 246 3079
Fragments arranged by size:
3079 2976 988 561 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8131
HpaI GTT'AAC
Cuts at: 5721 5721
Size: 8131
KasI G'GCGC_C
Cuts at: 2036 3804 2036
Size: 1768 6363
KpnI G_GTAC'C
Cuts at: 3814 5456 3814
Size: 1642 6489
MfeI C'AATT_G
Cuts at: 5730 5730
Size: 8131
MluI A'CGCG_T
Cuts at: 8007 8007
Size: 8131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5024 7245 7429 1305
Size: 3719 2221 184 2007
Fragments arranged by size:
3719 2221 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3172 5397 10
Size: 54 1425 343 177 1163 2225 2744
Fragments arranged by size:
2744 2225 1425 1163 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3284 3467 3977 4430 4544 4905 4988
Size: 648 2118 183 510 453 114 361 83
Cuts at: 4988 5024 5138 5426 6089 6448 6607 518
Size: 36 114 288 663 359 159 2042
Fragments arranged by size:
2118 2042 663 648 510 453 361 359
288 183 159 114 114 83 36
MspA1I CmG'CkG
Cuts at: 1606 3866 3977 4018 4045 4703 4978 5113 5221
Size: 2260 111 41 27 658 275 135 108
Cuts at: 5221 5547 6224 7372 7617 1606
Size: 326 677 1148 245 2120
Fragments arranged by size:
2260 2120 1148 677 658 326 275 245
135 111 108 41 27
NaeI GCC'GGC
Cuts at: 2747 5145 2747
Size: 2398 5733
NarI GG'CG_CC
Cuts at: 2037 3805 2037
Size: 1768 6363
NcoI C'CATG_G
Cuts at: 513 1065 2004 3109 3135 513
Size: 552 939 1105 26 5509
Fragments arranged by size:
5509 1105 939 552 26
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8131
NgoMIV G'CCGG_C
Cuts at: 2745 5143 2745
Size: 2398 5733
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8131
NotI GC'GGCC_GC
Cuts at: 5578 5578
Size: 8131
NsiI A_TGCA'T
Cuts at: 5202 5202
Size: 8131
NspI r_CATG'y
Cuts at: 1132 1229 3211 5425 5489 7034 1132
Size: 97 1982 2214 64 1545 2229
Fragments arranged by size:
2229 2214 1982 1545 97 64
PasI CC'CwG_GG
Cuts at: 1348 2540 2806 3698 4657 4834 1348
Size: 1192 266 892 959 177 4645
Fragments arranged by size:
4645 1192 959 892 266 177
PciI A'CATG_T
Cuts at: 7030 7030
Size: 8131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 3859 4093 1108
Size: 1758 993 234 5146
Fragments arranged by size:
5146 1758 993 234
PfoI T'CCnGG_A
Cuts at: 2382 2951 3357 3546 4959 5334 2382
Size: 569 406 189 1413 375 5179
Fragments arranged by size:
5179 1413 569 406 375 189
PmeI GTTT'AAAC
Cuts at: 5435 5435
Size: 8131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6200 6232 7713 7745 6200
Size: 32 1481 32 6586
Fragments arranged by size:
6586 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2176 2370 2481 3376 4090 4326 4440 2149
Size: 27 194 111 895 714 236 114 5840
Fragments arranged by size:
5840 895 714 236 194 114 111 27
PshAI GACnn'nnGTC
Cuts at: 1613 3662 1613
Size: 2049 6082
PsiI TTA'TAA
Cuts at: 5701 5701
Size: 8131
PspOMI G'GGCC_C
Cuts at: 2578 2712 3413 4314 4378 2578
Size: 134 701 901 64 6331
Fragments arranged by size:
6331 901 701 134 64
PstI C_TGCA'G
Cuts at: 830 2878 3325 5352 5483 830
Size: 2048 447 2027 131 3478
Fragments arranged by size:
3478 2048 2027 447 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8131
PvuII CAG'CTG
Cuts at: 5113 5221 5113
Size: 108 8023
RsrII CG'GwC_CG
Cuts at: 3388 4885 3388
Size: 1497 6634
SacI G_AGCT'C
Cuts at: 3065 4012 3065
Size: 947 7184
SalI G'TCGA_C
Cuts at: 1936 5473 1936
Size: 3537 4594
SanDI GG'GwC_CC
Cuts at: 2176 2370 2481 4326 2176
Size: 194 111 1845 5981
Fragments arranged by size:
5981 1845 194 111
SapI GCTCTTCn'nnn_
Cuts at: 3878 5336 3878
Size: 1458 6673
SbfI CC_TGCA'GG
Cuts at: 5483 5483
Size: 8131
ScaI AGT'ACT
Cuts at: 6379 8091 6379
Size: 1712 6419
SfcI C'TryA_G
Cuts at: 826 1045 2200 2874 3321 4605 5348 5479 6614
Size: 219 1155 674 447 1284 743 131 1135
Cuts at: 6614 7295 7486 826
Size: 681 191 1471
Fragments arranged by size:
1471 1284 1155 1135 743 681 674 447
219 191 131
SfoI GGC'GCC
Cuts at: 2038 3806 2038
Size: 1768 6363
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8131
SmaI CCC'GGG
Cuts at: 1872 2032 2711 2717 5458 1872
Size: 160 679 6 2741 4545
Fragments arranged by size:
4545 2741 679 160 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3371 4790 6235 7136 7398 820
Size: 197 926 1428 1419 1445 901 262 1553
Fragments arranged by size:
1553 1445 1428 1419 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2990 5922 493
Size: 2497 2932 2702
Fragments arranged by size:
2932 2702 2497
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8131
SphI G_CATG'C
Cuts at: 1132 5489 1132
Size: 4357 3774
SrfI GCCC'GGGC
Cuts at: 2717 2717
Size: 8131
SspI AAT'ATT
Cuts at: 5 52 6055 5
Size: 47 6003 2081
Fragments arranged by size:
6003 2081 47
StuI AGG'CCT
Cuts at: 1517 2535 1517
Size: 1018 7113
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2127 2672 3109 3135 3189
Size: 552 939 63 60 545 437 26 54
Cuts at: 3189 5178 5570 513
Size: 1989 392 3074
Fragments arranged by size:
3074 1989 939 552 545 437 392 63
60 54 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 3111 3950 4217 4299 4913 5233
Size: 1255 681 850 839 267 82 614 320
Cuts at: 5233 6158 6175 6328 6513 6958 325
Size: 925 17 153 185 445 1498
Fragments arranged by size:
1498 1255 925 850 839 681 614 445
320 267 185 153 82 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3352 5079 5406
Size: 274 80 33 51 1443 1375 1727 327
Cuts at: 5406 6377 8089 96
Size: 971 1712 138
Fragments arranged by size:
1727 1712 1443 1375 971 327 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 2774 2806 8091 8123 2388
Size: 32 354 32 5285 32 2396
Fragments arranged by size:
5285 2396 354 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8131
XbaI T'CTAG_A
Cuts at: 5467 5467
Size: 8131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3116 3165 4540 4546 3116
Size: 49 1375 6 6701
Fragments arranged by size:
6701 1375 49 6
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8131
XmaI C'CCGG_G
Cuts at: 1870 2030 2709 2715 5456 1870
Size: 160 679 6 2741 4545
Fragments arranged by size:
4545 2741 679 160 6
XmnI GAAnn'nnTTC
Cuts at: 6260 6260
Size: 8131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5939 276
Size: 53 83 186 936 4405 2468
Fragments arranged by size:
4405 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I EcoRV
FalI FseI FspAI NruI PacI PmlI PspXI PsrI
SacII SexAI SfiI SwaI