Restriction map for
pFN21AB3863
(Circular) (Six-base) MAPSORT of: pFN21AB3863.seq Check: 2479 from: 1 to: 7108
pFN21AB3863 7108 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3886 3886
Size: 7108
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4918 278
Size: 53 83 186 936 3382 2468
Fragments arranged by size:
3382 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2332 2684 4451 1937
Size: 395 352 1767 4594
Fragments arranged by size:
4594 1767 395 352
Acc65I G'GTAC_C
Cuts at: 4429 4429
Size: 7108
AclI AA'CG_TT
Cuts at: 1563 5235 5608 1563
Size: 3672 373 3063
Fragments arranged by size:
3672 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 3420 4365 5171 6555 6823 1632
Size: 771 1017 945 806 1384 268 1917
Fragments arranged by size:
1917 1384 1017 945 806 771 268
AfeI AGC'GCT
Cuts at: 2616 4106 4330 2616
Size: 1490 224 5394
Fragments arranged by size:
5394 1490 224
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6911
AflIII A'CryG_T
Cuts at: 1203 6007 6984 1203
Size: 4804 977 1327
Fragments arranged by size:
4804 1327 977
AgeI A'CCGG_T
Cuts at: 5939 5939
Size: 7108
AhdI GACnn_n'nnGTC
Cuts at: 5837 5837
Size: 7108
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3011 1184
Size: 1827 5281
AlwNI CAG_nnn'CTG
Cuts at: 4851 6423 4851
Size: 1572 5536
ApaI G_GGCC'C
Cuts at: 2075 2075
Size: 7108
ApaLI G'TGCA_C
Cuts at: 5165 6321 5165
Size: 1156 5952
ApoI r'AATT_y
Cuts at: 1493 4417 4643 4795 6904 1493
Size: 2924 226 152 2109 1697
Fragments arranged by size:
2924 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5662 160
Size: 872 4630 1606
Fragments arranged by size:
4630 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
AvaI C'yCGr_G
Cuts at: 1870 1943 4433 1870
Size: 73 2490 4545
Fragments arranged by size:
4545 2490 73
AvrII C'CTAG_G
Cuts at: 3731 3731
Size: 7108
BamHI G'GATC_C
Cuts at: 4438 4805 4438
Size: 367 6741
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2647 3736 3839 4429
Size: 325 217 627 368 492 1089 103 590
Cuts at: 4429 5884 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1089 627 590 492 368 325
217 103
BanII G_rGCy'C
Cuts at: 1391 2075 2141 3026 3473 3556 4026 4427 1391
Size: 684 66 885 447 83 470 401 4072
Fragments arranged by size:
4072 885 684 470 447 401 83 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3426 4371 928
Size: 971 1527 945 3665
Fragments arranged by size:
3665 1527 971 945
BbvCI CC'TCA_GC
Cuts at: 3191 3191
Size: 7108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5299 5333 1903
Size: 34 50 34 3278 34 3678
Fragments arranged by size:
3678 3278 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5000 6210 5000
Size: 1210 5898
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7108
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2302 2609 2885
Size: 107 122 71 631 605 630 307 276
Cuts at: 2885 3813 5719 136
Size: 928 1906 1525
Fragments arranged by size:
1906 1525 928 631 630 605 307 276
122 107 71
BglII A'GATC_T
Cuts at: 7103 7103
Size: 7108
BlpI GC'TnA_GC
Cuts at: 3562 3953 4525 3562
Size: 391 572 6145
Fragments arranged by size:
6145 572 391
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2641 3739 5169 6325 1790
Size: 285 566 1098 1430 1156 2573
Fragments arranged by size:
2573 1430 1156 1098 566 285
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2336 4839 5797
Size: 416 53 83 399 24 919 2503 958
Cuts at: 5797 442
Size: 1753
Fragments arranged by size:
2503 1753 958 919 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2907 1056
Size: 1851 5257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3490 4312 3103
Size: 387 822 5899
Fragments arranged by size:
5899 822 387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2603 2793 2883 3430 3849 4324 5768 1239
Size: 1364 190 90 547 419 475 1444 2579
Fragments arranged by size:
2579 1444 1364 547 475 419 190 90
Bpu10I CC'TnA_GC
Cuts at: 3191 4325 3191
Size: 1134 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 2942 4061 4121 5233 6098 6396 2741
Size: 201 1119 60 1112 865 298 3453
Fragments arranged by size:
3453 1119 1112 865 298 201 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5771 882
Size: 617 4272 2219
Fragments arranged by size:
4272 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4034 4127 4899 493
Size: 711 2830 93 772 2702
Fragments arranged by size:
2830 2702 772 711 93
BsaBI GATnn'nnATC
Cuts at: 7102 7102
Size: 7108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4915 5297 5932 275
Size: 53 83 186 936 3382 382 635 1451
Fragments arranged by size:
3382 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2335 5541 5939 6213 6360 6771 2335
Size: 3206 398 274 147 411 2672
Fragments arranged by size:
3206 2672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2769 2788 2799 2818 1178
Size: 30 165 30 1366 19 11 19 5468
Fragments arranged by size:
5468 1366 165 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2401 2679 2796 3463 3500 3503 3515 3524
Size: 1215 278 117 667 37 3 12 9
Cuts at: 3524 3920 3923 4348 4351 1186
Size: 396 3 425 3 3943
Fragments arranged by size:
3943 1215 667 425 396 278 117 37
12 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2062 2068 2814 3704 3988 6311 6807 2062
Size: 6 746 890 284 2323 496 2363
Fragments arranged by size:
2363 2323 890 746 496 284 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3126 4284 1163
Size: 573 1390 1158 3987
Fragments arranged by size:
3987 1390 1158 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4558 5319 6347 1540
Size: 275 186 2557 761 1028 2301
Fragments arranged by size:
2557 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3026 3473 5169 5254 6325 3026
Size: 447 1696 85 1071 3809
Fragments arranged by size:
3809 1696 1071 447 85
BsmI GAATG_Cn'
Cuts at: 2056 4619 4712 2056
Size: 2563 93 4452
Fragments arranged by size:
4452 2563 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2662 2662
Size: 7108
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2141 2641 3026 3473 3556 3739
Size: 399 285 66 500 385 447 83 183
Cuts at: 3739 4026 4427 5169 5254 6325 1391
Size: 287 401 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 447 401 399 385
287 285 183 85 83 66
BspEI T'CCGG_A
Cuts at: 6771 6771
Size: 7108
BspHI T'CATG_A
Cuts at: 4997 6727 4997
Size: 1730 5378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3886 4463 844
Size: 3042 577 3489
Fragments arranged by size:
3489 3042 577
BsrBI CCG'CTC
Cuts at: 1394 1823 2785 4995 1394
Size: 429 962 2210 3507
Fragments arranged by size:
3507 2210 962 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3058 3070 3321 5603 5777 65
Size: 1183 1810 12 251 2282 174 1396
Fragments arranged by size:
2282 1810 1396 1183 251 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 5752 5939 1700
Size: 110 3942 187 2869
Fragments arranged by size:
3942 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7108
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7108
BssSI C'ACGA_G
Cuts at: 5168 6180 5168
Size: 1012 6096
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7108
BstBI TT'CG_AA
Cuts at: 2104 5912 2104
Size: 3808 3300
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7108
BstYI r'GATC_y
Cuts at: 1972 4438 4805 5190 5207 6659 6745 6757 7103
Size: 2466 367 385 17 1452 86 12 346
Cuts at: 7103 1972
Size: 1977
Fragments arranged by size:
2466 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2685 2685
Size: 7108
Bsu36I CC'TnA_GG
Cuts at: 2928 2928
Size: 7108
BtgI C'CryG_G
Cuts at: 513 1065 3183 513
Size: 552 2118 4438
Fragments arranged by size:
4438 2118 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3542 487
Size: 683 56 2316 4053
Fragments arranged by size:
4053 2316 683 56
BtsI GCAGTG_nn'
Cuts at: 2632 3469 3787 3799 4320 4624 5418 5438 2632
Size: 837 318 12 521 304 794 20 4302
Fragments arranged by size:
4302 837 794 521 318 304 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7073
DraI TTT'AAA
Cuts at: 4412 4412
Size: 7108
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6115 809
Size: 507 4799 1802
Fragments arranged by size:
4799 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2294 3423 4555
Size: 54 1425 50 275 18 464 1129 1132
Cuts at: 4555 5444 7073 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1132 1129 889 464 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4555 1537
Size: 275 2743 4090
Fragments arranged by size:
4090 2743 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2665 3477 3513 3909 4044 4369 5038
Size: 737 575 812 36 396 135 325 669
Cuts at: 5038 1353
Size: 3423
Fragments arranged by size:
3423 812 737 669 575 396 325 135
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5695 6069 6215 387
Size: 1064 138 4106 374 146 1280
Fragments arranged by size:
4106 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3024 3471 3024
Size: 447 6661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2603 2793 2883 3420 3430 3849
Size: 393 771 200 190 90 537 10 419
Cuts at: 3849 4324 4365 5171 5768 6555 6823 1239
Size: 475 41 806 597 787 268 1524
Fragments arranged by size:
1524 806 787 771 597 537 475 419
393 268 200 190 90 41 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2043 2252 2528 3828 2043
Size: 209 276 1300 5323
Fragments arranged by size:
5323 1300 276 209
EcoO109I rG'GnC_Cy
Cuts at: 3386 3728 3806 3386
Size: 342 78 6688
Fragments arranged by size:
6688 342 78
EcoRI G'AATT_C
Cuts at: 4417 4795 4417
Size: 378 6730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3056 3056
Size: 7108
FspI TGC'GCA
Cuts at: 5614 5614
Size: 7108
HaeII r_GCGC'y
Cuts at: 1678 2618 4108 4332 6255 1678
Size: 940 1490 224 1923 2531
Fragments arranged by size:
2531 1923 1490 940 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2254 2286 2769 2801 4340
Size: 32 135 32 682 32 483 32 1539
Cuts at: 4340 4372 4385 4417 5749 5781 5823 5855 1373
Size: 32 13 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1539 1332 682 483 135 42 32
32 32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4452 4698 669
Size: 988 281 2514 246 3079
Fragments arranged by size:
3079 2514 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3038 748
Size: 2290 4818
HpaI GTT'AAC
Cuts at: 4698 4698
Size: 7108
KpnI G_GTAC'C
Cuts at: 4433 4433
Size: 7108
MfeI C'AATT_G
Cuts at: 4707 4707
Size: 7108
MluI A'CGCG_T
Cuts at: 6984 6984
Size: 7108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3297 6222 6406 1305
Size: 1992 2925 184 2007
Fragments arranged by size:
2925 2007 1992 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2296 3425 10
Size: 54 1425 343 464 1129 3693
Fragments arranged by size:
3693 1425 1129 464 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3063 5066 5425 5584 518
Size: 648 1897 2003 359 159 2042
Fragments arranged by size:
2042 2003 1897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2239 3952 4236 4524 5201 6349 6594 1606
Size: 633 1713 284 288 677 1148 245 2120
Fragments arranged by size:
2120 1713 1148 677 633 288 284 245
NcoI C'CATG_G
Cuts at: 513 1065 3183 513
Size: 552 2118 4438
Fragments arranged by size:
4438 2118 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7108
NheI G'CTAG_C
Cuts at: 1052 2903 1052
Size: 1851 5257
NotI GC'GGCC_GC
Cuts at: 4555 4555
Size: 7108
NspI r_CATG'y
Cuts at: 1132 1229 3101 4466 6011 1132
Size: 97 1872 1365 1545 2229
Fragments arranged by size:
2229 1872 1545 1365 97
PasI CC'CwG_GG
Cuts at: 1348 2039 3619 1348
Size: 691 1580 4837
Fragments arranged by size:
4837 1580 691
PciI A'CATG_T
Cuts at: 6007 6007
Size: 7108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7108
PmeI GTTT'AAAC
Cuts at: 4412 4412
Size: 7108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5177 5209 6690 6722 5177
Size: 32 1481 32 5563
Fragments arranged by size:
5563 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7108
PsiI TTA'TAA
Cuts at: 2354 4678 2354
Size: 2324 4784
PspOMI G'GGCC_C
Cuts at: 2071 2071
Size: 7108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 7076
PstI C_TGCA'G
Cuts at: 830 2803 4460 830
Size: 1973 1657 3478
Fragments arranged by size:
3478 1973 1657
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7108
PvuII CAG'CTG
Cuts at: 2239 3952 2239
Size: 1713 5395
SacI G_AGCT'C
Cuts at: 3026 3473 3026
Size: 447 6661
SalI G'TCGA_C
Cuts at: 1936 4450 1936
Size: 2514 4594
SbfI CC_TGCA'GG
Cuts at: 4460 4460
Size: 7108
ScaI AGT'ACT
Cuts at: 5356 7068 5356
Size: 1712 5396
SfcI C'TryA_G
Cuts at: 826 1045 2525 2799 2874 3329 3572 3888 4456
Size: 219 1480 274 75 455 243 316 568
Cuts at: 4456 5591 6272 6463 826
Size: 1135 681 191 1471
Fragments arranged by size:
1480 1471 1135 681 568 455 316 274
243 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7108
SmaI CCC'GGG
Cuts at: 1872 4435 1872
Size: 2563 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 2921 4076 4100 5212 6113
Size: 197 926 813 165 1155 24 1112 901
Cuts at: 6113 6375 820
Size: 262 1553
Fragments arranged by size:
1553 1155 1112 926 901 813 262 197
165 24
SnaBI TAC'GTA
Cuts at: 493 4127 4899 493
Size: 3634 772 2702
Fragments arranged by size:
3634 2702 772
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7108
SphI G_CATG'C
Cuts at: 1132 3101 4466 1132
Size: 1969 1365 3774
Fragments arranged by size:
3774 1969 1365
SspI AAT'ATT
Cuts at: 5 52 5032 5
Size: 47 4980 2081
Fragments arranged by size:
4980 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7108
StyI C'CwwG_G
Cuts at: 513 1065 3183 3731 4547 513
Size: 552 2118 548 816 3074
Fragments arranged by size:
3074 2118 816 552 548
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2745 5135 5152 5305 5490 5935 325
Size: 1255 1165 2390 17 153 185 445 1498
Fragments arranged by size:
2390 1498 1255 1165 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 3592 5354
Size: 274 80 33 51 1443 1308 307 1762
Cuts at: 5354 7066 96
Size: 1712 138
Fragments arranged by size:
1762 1712 1443 1308 307 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7068 7100 7068
Size: 32 7076
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7108
XbaI T'CTAG_A
Cuts at: 4444 4444
Size: 7108
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7108
XmaI C'CCGG_G
Cuts at: 1870 4433 1870
Size: 2563 4545
XmnI GAAnn'nnTTC
Cuts at: 3040 3582 5237 3040
Size: 542 1655 4911
Fragments arranged by size:
4911 1655 542
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4916 276
Size: 53 83 186 936 3382 2468
Fragments arranged by size:
3382 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BstXI DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI