Restriction map for
pFN21AB3860
(Circular) (Six-base) MAPSORT of: pFN21AB3860.seq Check: 3201 from: 1 to: 5749
pFN21AB3860 5749 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2549 2733 2549
Size: 184 5565
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 3092 1937
Size: 217 938 4594
Fragments arranged by size:
4594 938 217
Acc65I G'GTAC_C
Cuts at: 2121 3070 2121
Size: 949 4800
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 5196 5464 1632
Size: 2180 1384 268 1917
Fragments arranged by size:
2180 1917 1384 268
AfeI AGC'GCT
Cuts at: 2927 2927
Size: 5749
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 2043 2577 2697 4648 5625 1203
Size: 840 534 120 1951 977 1327
Fragments arranged by size:
1951 1327 977 840 534 120
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2219 2255 2564 3492 5064 2219
Size: 36 309 928 1572 2904
Fragments arranged by size:
2904 1572 928 309 36
ApaI G_GGCC'C
Cuts at: 2145 2145
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AscI GG'CGCG_CC
Cuts at: 2100 2100
Size: 5749
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 2486 3079 3446 2486
Size: 593 367 4789
Fragments arranged by size:
4789 593 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2528 2996 3070 4525
Size: 325 217 627 334 407 468 74 1455
Cuts at: 4525 618
Size: 1842
Fragments arranged by size:
1842 1455 627 468 407 334 325 217
74
BanII G_rGCy'C
Cuts at: 1391 2145 2205 3068 1391
Size: 754 60 863 4072
Fragments arranged by size:
4072 863 754 60
BbeI G_GCGC'C
Cuts at: 3000 3000
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BbvCI CC'TCA_GC
Cuts at: 2126 2126
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2077 2111 2839 2873 3940
Size: 34 50 34 56 34 728 34 1067
Cuts at: 3940 3974 1903
Size: 34 3678
Fragments arranged by size:
3678 1067 728 56 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2429 2508 4360
Size: 107 122 71 631 605 757 79 1852
Cuts at: 4360 136
Size: 1525
Fragments arranged by size:
1852 1525 757 631 605 122 107 79
71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3032 3166 3032
Size: 134 5615
Bme1580I G_kGCm'C
Cuts at: 1790 2145 3810 4966 1790
Size: 355 1665 1156 2573
Fragments arranged by size:
2573 1665 1156 355
BmgBI CAC'GTC
Cuts at: 1318 2610 2682 1318
Size: 1292 72 4385
Fragments arranged by size:
4385 1292 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 4409 1239
Size: 845 2325 2579
Fragments arranged by size:
2579 2325 845
Bpu10I CC'TnA_GC
Cuts at: 2126 2621 2126
Size: 495 5254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2933 3874 4739 5037 2933
Size: 941 865 298 3645
Fragments arranged by size:
3645 941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 2698 3540 493
Size: 711 933 561 842 2702
Fragments arranged by size:
2702 933 842 711 561
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2349 2448 2997
Size: 53 83 186 936 633 183 99 549
Cuts at: 2997 3556 3938 4573 275
Size: 559 382 635 1451
Fragments arranged by size:
1451 936 635 633 559 549 382 186
183 99 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 4705
Fragments arranged by size:
4705 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5749
BseYI C'CCAG_C
Cuts at: 2233 4952 5448 2233
Size: 2719 496 2534
Fragments arranged by size:
2719 2534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 3062 1163
Size: 573 357 969 3850
Fragments arranged by size:
3850 969 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2325 2572 2866 3199 3960
Size: 275 186 57 267 247 294 333 761
Cuts at: 3960 4988 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 333 294 275 267 247
186 57
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 2896 3260 3353 2896
Size: 364 93 5292
Fragments arranged by size:
5292 364 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2205 3068 3810 3895 4966 1391
Size: 399 355 60 863 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 355 85 60
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2549 2733 2748 2883 3104 844
Size: 1705 184 15 135 221 3489
Fragments arranged by size:
3489 1705 221 184 135 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2411 2868 3636 1394
Size: 429 588 457 768 3507
Fragments arranged by size:
3507 768 588 457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2396 2571 2707 2816 4393 4580 1700
Size: 110 586 175 136 109 1577 187 2869
Fragments arranged by size:
2869 1577 586 187 175 136 110 109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 2100 2515 2537 2539 2838 2878 2880 1925
Size: 175 415 22 2 299 40 2 4794
Fragments arranged by size:
4794 415 299 175 40 22 2 2
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2255 2740 2928 794
Size: 1461 485 188 3615
Fragments arranged by size:
3615 1461 485 188
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2486 3079 3446 3831 3848 5300 5386 5398
Size: 514 593 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 593 514 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2712 3009 513
Size: 552 1647 297 3253
Fragments arranged by size:
3253 1647 552 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2105 2842 3265 4059 4079 2105
Size: 737 423 794 20 3775
Fragments arranged by size:
3775 794 737 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 4756 809
Size: 507 1008 2432 1802
Fragments arranged by size:
2432 1802 1008 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2286 2322
Size: 54 1425 50 275 18 225 231 36
Cuts at: 2322 2569 2709 2715 3196 4085 5714 8
Size: 247 140 6 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 275 247 231 225
140 54 50 43 36 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2322 2569 3196 1537
Size: 275 243 267 247 627 4090
Fragments arranged by size:
4090 627 275 267 247 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2056 2930 3679 1353
Size: 703 874 749 3423
Fragments arranged by size:
3423 874 749 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2360 4336 4710 4856 387
Size: 1064 138 771 1976 374 146 1280
Fragments arranged by size:
1976 1280 1064 771 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 3812 4409 5196 5464 1239
Size: 393 452 1728 597 787 268 1524
Fragments arranged by size:
1728 1524 787 597 452 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2013 2141 2142 3005 2013
Size: 128 1 863 4757
Fragments arranged by size:
4757 863 128 1
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 5749
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2073 2374 2674 2929 3000 4896 1678
Size: 395 301 300 255 71 1896 2531
Fragments arranged by size:
2531 1896 395 301 300 255 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2357 2389 4390
Size: 32 135 32 461 32 292 32 2001
Cuts at: 4390 4422 4464 4496 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2001 461 292 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2996 2996
Size: 5749
KpnI G_GTAC'C
Cuts at: 2125 3074 2125
Size: 949 4800
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 2043 2577 5625 2043
Size: 534 3048 2167
Fragments arranged by size:
3048 2167 534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 4863 5047 1305
Size: 1590 1968 184 2007
Fragments arranged by size:
2007 1968 1590 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 2717 10
Size: 54 1425 343 456 429 3042
Fragments arranged by size:
3042 1425 456 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2717 3707 4066 4225 518
Size: 648 1551 990 359 159 2042
Fragments arranged by size:
2042 1551 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2219 2294 2662 3011 3165 3842 4990 5235
Size: 613 75 368 349 154 677 1148 245
Cuts at: 5235 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 613 368 349 245 154
75
NaeI GCC'GGC
Cuts at: 2398 2573 2709 2398
Size: 175 136 5438
Fragments arranged by size:
5438 175 136
NarI GG'CG_CC
Cuts at: 2997 2997
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 2712 513
Size: 552 1647 3550
Fragments arranged by size:
3550 1647 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NgoMIV G'CCGG_C
Cuts at: 2396 2571 2707 2396
Size: 175 136 5438
Fragments arranged by size:
5438 175 136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2806 2890 3107 4652 1132
Size: 97 1577 84 217 1545 2229
Fragments arranged by size:
2229 1577 1545 217 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2013 2013
Size: 5749
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2141 2141
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2251 2796 3101 830
Size: 1421 545 305 3478
Fragments arranged by size:
3478 1421 545 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2219 2662 2219
Size: 443 5306
SacII CC_GC'GG
Cuts at: 3012 3012
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SapI GCTCTTCn'nnn_
Cuts at: 2930 2930
Size: 5749
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2247 2792 3097 4232 4913 5104 826
Size: 219 1202 545 305 1135 681 191 1471
Fragments arranged by size:
1471 1202 1135 681 545 305 219 191
SfoI GGC'GCC
Cuts at: 2998 2998
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 2816 1700
Size: 1116 4633
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2948 3853 4754 5016 820
Size: 197 926 1005 905 901 262 1553
Fragments arranged by size:
1553 1005 926 905 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 2855 1517
Size: 1338 4411
StyI C'CwwG_G
Cuts at: 513 1065 2712 3188 513
Size: 552 1647 476 3074
Fragments arranged by size:
3074 1647 552 476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2710 2017
Size: 693 5056
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 2828 2939 3878 2828
Size: 111 939 4699
Fragments arranged by size:
4699 939 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SrfI SwaI