Restriction map for
pFN21AB3853
(Circular) (Six-base) MAPSORT of: pFN21AB3853.seq Check: 394 from: 1 to: 5341
pFN21AB3853 5341 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 2684 1937
Size: 271 476 4594
Fragments arranged by size:
4594 476 271
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2452 2619 3404 4788 5056 1632
Size: 820 167 785 1384 268 1917
Fragments arranged by size:
1917 1384 820 785 268 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 2203 4172 2203
Size: 1969 3372
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2386 1184
Size: 1202 4139
AlwNI CAG_nnn'CTG
Cuts at: 2306 2374 3084 4656 2306
Size: 68 710 1572 2991
Fragments arranged by size:
2991 1572 710 68
ApaI G_GGCC'C
Cuts at: 2382 2382
Size: 5341
ApaLI G'TGCA_C
Cuts at: 2320 3398 4554 2320
Size: 1078 1156 3107
Fragments arranged by size:
3107 1156 1078
ApoI r'AATT_y
Cuts at: 1493 2126 2441 2650 2876 3028 5137 1493
Size: 633 315 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 633 315 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2165 2671 3038 2165
Size: 506 367 4468
Fragments arranged by size:
4468 506 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2382 2660 1391
Size: 991 278 4072
Fragments arranged by size:
4072 991 278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 2416 2625 928
Size: 971 445 72 209 3644
Fragments arranged by size:
3644 971 445 209 72
BbvCI CC'TCA_GC
Cuts at: 2156 2156
Size: 5341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2324 2382 3402 4558 1790
Size: 534 58 1020 1156 2573
Fragments arranged by size:
2573 1156 1020 534 58
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2188 2362 3072
Size: 416 53 83 399 24 771 174 710
Cuts at: 3072 4030 442
Size: 958 1753
Fragments arranged by size:
1753 958 771 710 416 399 174 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 2414
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2580 2640 4001 1239
Size: 1341 60 1361 2579
Fragments arranged by size:
2579 1361 1341 60
Bpu10I CC'TnA_GC
Cuts at: 2156 2156
Size: 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2082 3466 4331 4629 2082
Size: 1384 865 298 2794
Fragments arranged by size:
2794 1384 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 2336 5335 2336
Size: 2999 2342
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2203 3774 4172 4446 4593 5004 2203
Size: 1571 398 274 147 411 2540
Fragments arranged by size:
2540 1571 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2609 1186
Size: 958 465 3918
Fragments arranged by size:
3918 958 465
BseYI C'CCAG_C
Cuts at: 2481 4544 5040 2481
Size: 2063 496 2782
Fragments arranged by size:
2782 2063 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 1163
Size: 573 543 4225
Fragments arranged by size:
4225 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2208 2791 3552 4580 1540
Size: 275 186 207 583 761 1028 2301
Fragments arranged by size:
2301 1028 761 583 275 207 186
BsiHKAI G_wGCw'C
Cuts at: 2324 3402 3487 4558 2324
Size: 1078 85 1071 3107
Fragments arranged by size:
3107 1078 1071 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
BsmBI CGTCTCn'nnnn_
Cuts at: 2528 2528
Size: 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2324 2382 2660 3402 3487 4558 1391
Size: 399 534 58 278 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 399 278 85 58
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2244 3836 4010 65
Size: 1183 996 1592 174 1396
Fragments arranged by size:
1592 1396 1183 996 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2203 3985 4172 1700
Size: 110 393 1782 187 2869
Fragments arranged by size:
2869 1782 393 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2165 2671 3038 3423 3440 4892 4978 4990
Size: 193 506 367 385 17 1452 86 12
Cuts at: 4990 5336 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 506 385 367 346 193 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2253 2313 513
Size: 552 1188 60 3541
Fragments arranged by size:
3541 1188 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2323 2344 487
Size: 683 56 1097 21 3484
Fragments arranged by size:
3484 1097 683 56 21
BtsI GCAGTG_nn'
Cuts at: 2267 2307 2857 3651 3671 2267
Size: 40 550 794 20 3937
Fragments arranged by size:
3937 794 550 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2164 2199 529
Size: 35 1600 35 3671
Fragments arranged by size:
3671 1600 35 35
DraI TTT'AAA
Cuts at: 2458 2645 2458
Size: 187 5154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2269 2284
Size: 54 1425 50 275 18 303 136 15
Cuts at: 2284 2310 2788 3677 5306 8
Size: 26 478 889 1629 43
Fragments arranged by size:
1629 1425 889 478 303 275 136 54
50 43 26 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3271 1353
Size: 1918 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2452 2580 2619 2640 3404 4001 4788
Size: 393 820 128 39 21 764 597 787
Cuts at: 4788 5056 1239
Size: 268 1524
Fragments arranged by size:
1524 820 787 764 597 393 268 128
39 21
EcoO109I rG'GnC_Cy
Cuts at: 2378 2378
Size: 5341
EcoRI G'AATT_C
Cuts at: 2441 2650 3028 2441
Size: 209 378 4754
Fragments arranged by size:
4754 378 209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5341
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2052 2084 2157 2189 2615
Size: 32 135 32 480 32 73 32 426
Cuts at: 2615 2647 3982 4014 4056 4088 1373
Size: 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1335 480 426 135 73 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2584 2685 2931 669
Size: 988 281 271 375 101 246 3079
Fragments arranged by size:
3079 988 375 281 271 246 101
HindIII A'AGCT_T
Cuts at: 748 2100 748
Size: 1352 3989
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2271 2286 2312 10
Size: 54 1425 343 303 136 15 26 3039
Fragments arranged by size:
3039 1425 343 303 136 54 26 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2507 3299 3658 3817 518
Size: 648 906 435 792 359 159 2042
Fragments arranged by size:
2042 906 792 648 435 359 159
MspA1I CmG'CkG
Cuts at: 1606 2123 2280 2757 3434 4582 4827 1606
Size: 517 157 477 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 517 477 245 157
NcoI C'CATG_G
Cuts at: 513 1065 2253 2313 513
Size: 552 1188 60 3541
Fragments arranged by size:
3541 1188 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2573 2699 4244 1132
Size: 97 1344 126 1545 2229
Fragments arranged by size:
2229 1545 1344 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2178 2374 1108
Size: 1070 196 4075
Fragments arranged by size:
4075 1070 196
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PspOMI G'GGCC_C
Cuts at: 2378 2378
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2302 2693 830
Size: 1472 391 3478
Fragments arranged by size:
3478 1472 391
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2207 2683 1936
Size: 271 476 4594
Fragments arranged by size:
4594 476 271
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2298 2689 3824 4505 4696 826
Size: 219 1253 391 1135 681 191 1471
Fragments arranged by size:
1471 1253 1135 681 391 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2097 3445 4346 4608 820
Size: 197 926 154 1348 901 262 1553
Fragments arranged by size:
1553 1348 926 901 262 197 154
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2253 2313 2780 513
Size: 552 1188 60 467 3074
Fragments arranged by size:
3074 1188 552 467 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2279 2279
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI