Restriction map for
pFN21AB3844
(Circular) (Six-base) MAPSORT of: pFN21AB3844.seq Check: 1948 from: 1 to: 6727
pFN21AB3844 6727 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2171 2412 2826 2171
Size: 241 414 6072
Fragments arranged by size:
6072 414 241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4537 278
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4070 1937
Size: 2133 4594
Acc65I G'GTAC_C
Cuts at: 4048 4048
Size: 6727
AclI AA'CG_TT
Cuts at: 1563 4854 5227 1563
Size: 3291 373 3063
Fragments arranged by size:
3291 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2719 4790 6174 6442 1632
Size: 1087 2071 1384 268 1917
Fragments arranged by size:
2071 1917 1384 1087 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6530
AflIII A'CryG_T
Cuts at: 1203 5626 6603 1203
Size: 4423 977 1327
Fragments arranged by size:
4423 1327 977
AgeI A'CCGG_T
Cuts at: 5558 5558
Size: 6727
AhdI GACnn_n'nnGTC
Cuts at: 5456 5456
Size: 6727
AleI CACnn'nnGTG
Cuts at: 1166 2069 3571 1166
Size: 903 1502 4322
Fragments arranged by size:
4322 1502 903
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2474 2859 1184
Size: 1290 385 5052
Fragments arranged by size:
5052 1290 385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2093 2125 2093
Size: 32 6695
AlwNI CAG_nnn'CTG
Cuts at: 2974 3868 3942 4470 6042 2974
Size: 894 74 528 1572 3659
Fragments arranged by size:
3659 1572 894 528 74
ApaI G_GGCC'C
Cuts at: 3083 3083
Size: 6727
ApaLI G'TGCA_C
Cuts at: 3734 4784 5940 3734
Size: 1050 1156 4521
Fragments arranged by size:
4521 1156 1050
ApoI r'AATT_y
Cuts at: 1493 4036 4262 4414 6523 1493
Size: 2543 226 152 2109 1697
Fragments arranged by size:
2543 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5281 160
Size: 872 4249 1606
Fragments arranged by size:
4249 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
AvaI C'yCGr_G
Cuts at: 1870 1943 2584 3759 4052 1870
Size: 73 641 1175 293 4545
Fragments arranged by size:
4545 1175 641 293 73
BamHI G'GATC_C
Cuts at: 4057 4424 4057
Size: 367 6360
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2679 3308 3971 4048 5503
Size: 325 217 627 892 629 663 77 1455
Cuts at: 5503 618
Size: 1842
Fragments arranged by size:
1842 1455 892 663 629 627 325 217
77
BanII G_rGCy'C
Cuts at: 1391 2127 2763 3083 3440 3501 4046 1391
Size: 736 636 320 357 61 545 4072
Fragments arranged by size:
4072 736 636 545 357 320 61
BbeI G_GCGC'C
Cuts at: 2683 3312 2683
Size: 629 6098
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3688 928
Size: 971 1789 3967
Fragments arranged by size:
3967 1789 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4918 4952 1903
Size: 34 2981 34 3678
Fragments arranged by size:
3678 2981 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4619 5829 4619
Size: 1210 5517
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3173 3344 5338
Size: 107 122 71 631 605 1501 171 1994
Cuts at: 5338 136
Size: 1525
Fragments arranged by size:
1994 1525 1501 631 605 171 122 107
71
BglII A'GATC_T
Cuts at: 6722 6722
Size: 6727
BlpI GC'TnA_GC
Cuts at: 4144 4144
Size: 6727
Bme1580I G_kGCm'C
Cuts at: 1790 3083 3738 4788 5944 1790
Size: 1293 655 1050 1156 2573
Fragments arranged by size:
2573 1293 1156 1050 655
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6727
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3037 4458 5416
Size: 416 53 83 399 24 1620 1421 958
Cuts at: 5416 442
Size: 1753
Fragments arranged by size:
1753 1620 1421 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2624 2912 3515 5387 1239
Size: 1244 141 288 603 1872 2579
Fragments arranged by size:
2579 1872 1244 603 288 141
Bpu10I CC'TnA_GC
Cuts at: 2555 2570 2978 2555
Size: 15 408 6304
Fragments arranged by size:
6304 408 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2525 3224 4852 5717 6015 2294
Size: 231 699 1628 865 298 3006
Fragments arranged by size:
3006 1628 865 699 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3443 3530 5390 882
Size: 617 1944 87 1860 2219
Fragments arranged by size:
2219 1944 1860 617 87
BsaAI yAC'GTr
Cuts at: 493 1204 3331 3366 3553 3733 3904 4518 493
Size: 711 2127 35 187 180 171 614 2702
Fragments arranged by size:
2702 2127 711 614 187 180 171 35
BsaBI GATnn'nnATC
Cuts at: 6721 6721
Size: 6727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2517 2680 3309
Size: 53 83 186 936 717 267 163 629
Cuts at: 3309 4534 4916 5551 275
Size: 1225 382 635 1451
Fragments arranged by size:
1451 1225 936 717 635 629 382 267
186 163 83 53
BsaWI w'CCGG_w
Cuts at: 5160 5558 5832 5979 6390 5160
Size: 398 274 147 411 5497
Fragments arranged by size:
5497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2618 2648 3488 3518 1178
Size: 30 165 30 1215 30 840 30 4387
Fragments arranged by size:
4387 1215 840 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 2621 3379 3911 1186
Size: 1339 96 758 532 4002
Fragments arranged by size:
4002 1339 758 532 96
BseYI C'CCAG_C
Cuts at: 2807 2967 3427 5930 6426 2807
Size: 160 460 2503 496 3108
Fragments arranged by size:
3108 2503 496 460 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2384 2456 3479 1163
Size: 573 612 36 72 1023 4411
Fragments arranged by size:
4411 1023 612 573 72 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2212 3048 4177 4938 5966 1540
Size: 275 186 211 836 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 836 761 275 211 186
BsiHKAI G_wGCw'C
Cuts at: 2763 3738 4788 4873 5944 2763
Size: 975 1050 85 1071 3546
Fragments arranged by size:
3546 1071 1050 975 85
BsmI GAATG_Cn'
Cuts at: 4238 4331 4238
Size: 93 6634
BsmBI CGTCTCn'nnnn_
Cuts at: 3137 3137
Size: 6727
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2763 3083 3440 3501 3738 4046
Size: 399 337 636 320 357 61 237 308
Cuts at: 4046 4788 4873 5944 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 399 357 337 320
308 237 85 61
BspEI T'CCGG_A
Cuts at: 6390 6390
Size: 6727
BspHI T'CATG_A
Cuts at: 4616 6346 4616
Size: 1730 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2171 2274 2320 2385 2412 2709 2811
Size: 1259 68 103 46 65 27 297 102
Cuts at: 2811 2826 2841 3489 4082 844
Size: 15 15 648 593 3489
Fragments arranged by size:
3489 1259 648 593 297 103 102 68
65 46 27 15 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2385 2896 4614 1394
Size: 429 562 511 1718 3507
Fragments arranged by size:
3507 1718 562 511 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 3550 5222 5396 65
Size: 1183 1179 1123 1672 174 1396
Fragments arranged by size:
1672 1396 1183 1179 1123 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5371 5558 1700
Size: 110 3561 187 2869
Fragments arranged by size:
3561 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6727
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6727
BssSI C'ACGA_G
Cuts at: 4787 5799 4787
Size: 1012 5715
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2163 3868 3942 794
Size: 1369 1705 74 3579
Fragments arranged by size:
3579 1705 1369 74
BstBI TT'CG_AA
Cuts at: 5531 5531
Size: 6727
BstEII G'GTnAC_C
Cuts at: 1184 2696 3526 1184
Size: 1512 830 4385
Fragments arranged by size:
4385 1512 830
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3127 3319 2011
Size: 1116 192 5419
Fragments arranged by size:
5419 1116 192
BstYI r'GATC_y
Cuts at: 1972 4057 4424 4809 4826 6278 6364 6376 6722
Size: 2085 367 385 17 1452 86 12 346
Cuts at: 6722 1972
Size: 1977
Fragments arranged by size:
2085 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2088 2935 3048 3186 3291
Size: 552 939 75 9 847 113 138 105
Cuts at: 3291 3297 3432 3531 3645 3847 513
Size: 6 135 99 114 202 3393
Fragments arranged by size:
3393 939 847 552 202 138 135 114
113 105 99 75 9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2113 2704 487
Size: 683 56 887 591 4510
Fragments arranged by size:
4510 887 683 591 56
BtsI GCAGTG_nn'
Cuts at: 2028 2046 3690 4243 5037 5057 2028
Size: 18 1644 553 794 20 3698
Fragments arranged by size:
3698 1644 794 553 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6692
DraI TTT'AAA
Cuts at: 4031 4031
Size: 6727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5734 809
Size: 507 4418 1802
Fragments arranged by size:
4418 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2082 2706
Size: 54 1425 50 275 18 231 21 624
Cuts at: 2706 2784 2874 3045 3102 3165 3189 3294 3321
Size: 78 90 171 57 63 24 105 27
Cuts at: 3321 4174 5063 6692 8
Size: 853 889 1629 43
Fragments arranged by size:
1629 1425 889 853 624 275 231 171
105 90 78 63 57 54 50 43
27 24 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3045 4174 1537
Size: 275 1233 1129 4090
Fragments arranged by size:
4090 1233 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2555 3707 4657 1353
Size: 1202 1152 950 3423
Fragments arranged by size:
3423 1202 1152 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5314 5688 5834 387
Size: 1064 138 3725 374 146 1280
Fragments arranged by size:
3725 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2761 2761
Size: 6727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2483 2624 2719 2912 3515 4790 5387
Size: 393 851 141 95 193 603 1275 597
Cuts at: 5387 6174 6442 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1275 851 787 603 597 393 268
193 141 95
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 3944 2141
Size: 1803 4924
EcoO109I rG'GnC_Cy
Cuts at: 2305 2662 3064 3079 3080 3337 3768 2305
Size: 357 402 15 1 257 431 5264
Fragments arranged by size:
5264 431 402 357 257 15 1
EcoRI G'AATT_C
Cuts at: 4036 4414 4036
Size: 378 6349
EcoRV GAT'ATC
Cuts at: 2239 2239
Size: 6727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 6727
FspI TGC'GCA
Cuts at: 2423 2774 5233 2423
Size: 351 2459 3917
Fragments arranged by size:
3917 2459 351
FspAI rTGC'GCAy
Cuts at: 2423 2423
Size: 6727
HaeII r_GCGC'y
Cuts at: 1678 2683 2814 3312 5874 1678
Size: 1005 131 498 2562 2531
Fragments arranged by size:
2562 2531 1005 498 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 3488 3520 5368
Size: 32 135 32 899 32 985 32 1848
Cuts at: 5368 5400 5442 5474 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1848 985 899 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2323 3055 4071 4317 669
Size: 988 281 385 732 1016 246 3079
Fragments arranged by size:
3079 1016 988 732 385 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6727
HpaI GTT'AAC
Cuts at: 4317 4317
Size: 6727
KasI G'GCGC_C
Cuts at: 2679 3308 2679
Size: 629 6098
KpnI G_GTAC'C
Cuts at: 4052 4052
Size: 6727
MfeI C'AATT_G
Cuts at: 4326 4326
Size: 6727
MluI A'CGCG_T
Cuts at: 6603 6603
Size: 6727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2934 3300 3951 5841 6025 1305
Size: 1629 366 651 1890 184 2007
Fragments arranged by size:
2007 1890 1629 651 366 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 2876 3104 3167 3296
Size: 54 1425 343 876 168 228 63 129
Cuts at: 3296 3323 10
Size: 27 3414
Fragments arranged by size:
3414 1425 876 343 228 168 129 63
54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2069 2478 3329 3521 3571 4685
Size: 648 843 60 409 851 192 50 1114
Cuts at: 4685 5044 5203 518
Size: 359 159 2042
Fragments arranged by size:
2042 1114 851 843 648 409 359 192
159 60 50
MspA1I CmG'CkG
Cuts at: 1606 2181 2458 2647 2937 2971 3849 4143 4820
Size: 575 277 189 290 34 878 294 677
Cuts at: 4820 5968 6213 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 878 677 575 294 290 277
245 189 34
NarI GG'CG_CC
Cuts at: 2680 3309 2680
Size: 629 6098
NcoI C'CATG_G
Cuts at: 513 1065 2004 2088 3291 3531 3645 513
Size: 552 939 84 1203 240 114 3595
Fragments arranged by size:
3595 1203 939 552 240 114 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6727
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6727
NotI GC'GGCC_GC
Cuts at: 4174 4174
Size: 6727
NspI r_CATG'y
Cuts at: 1132 1229 2654 4085 5630 1132
Size: 97 1425 1431 1545 2229
Fragments arranged by size:
2229 1545 1431 1425 97
PasI CC'CwG_GG
Cuts at: 1348 3220 3854 1348
Size: 1872 634 4221
Fragments arranged by size:
4221 1872 634
PciI A'CATG_T
Cuts at: 5626 5626
Size: 6727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3726 1108
Size: 2618 4109
PfoI T'CCnGG_A
Cuts at: 2391 3837 2391
Size: 1446 5281
PmeI GTTT'AAAC
Cuts at: 4031 4031
Size: 6727
PmlI CAC'GTG
Cuts at: 3331 3366 3331
Size: 35 6692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4796 4828 6309 6341 4796
Size: 32 1481 32 5182
Fragments arranged by size:
5182 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 6727
PshAI GACnn'nnGTC
Cuts at: 1613 2144 2318 1613
Size: 531 174 6022
Fragments arranged by size:
6022 531 174
PsiI TTA'TAA
Cuts at: 4297 4297
Size: 6727
PspOMI G'GGCC_C
Cuts at: 3079 3079
Size: 6727
PstI C_TGCA'G
Cuts at: 830 2271 2409 2823 2838 3864 4079 830
Size: 1441 138 414 15 1026 215 3478
Fragments arranged by size:
3478 1441 1026 414 215 138 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6727
PvuII CAG'CTG
Cuts at: 2971 2971
Size: 6727
SacI G_AGCT'C
Cuts at: 2763 2763
Size: 6727
SacII CC_GC'GG
Cuts at: 2938 3850 2938
Size: 912 5815
SalI G'TCGA_C
Cuts at: 1936 4069 1936
Size: 2133 4594
SapI GCTCTTCn'nnn_
Cuts at: 2555 2555
Size: 6727
SbfI CC_TGCA'GG
Cuts at: 2409 2838 4079 2409
Size: 429 1241 5057
Fragments arranged by size:
5057 1241 429
ScaI AGT'ACT
Cuts at: 4975 6687 4975
Size: 1712 5015
SfcI C'TryA_G
Cuts at: 826 1045 2267 2405 2744 2819 2834 3860 4075
Size: 219 1222 138 339 75 15 1026 215
Cuts at: 4075 5210 5891 6082 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1222 1135 1026 681 339 219 215
191 138 75 15
SfoI GGC'GCC
Cuts at: 2681 3310 2681
Size: 629 6098
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6727
SmaI CCC'GGG
Cuts at: 1872 3761 4054 1872
Size: 1889 293 4545
Fragments arranged by size:
4545 1889 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 2540 3239 4831 5732 5994
Size: 197 926 366 231 699 1592 901 262
Cuts at: 5994 820
Size: 1553
Fragments arranged by size:
1592 1553 926 901 699 366 262 231
197
SnaBI TAC'GTA
Cuts at: 493 3553 4518 493
Size: 3060 965 2702
Fragments arranged by size:
3060 2702 965
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6727
SphI G_CATG'C
Cuts at: 1132 2654 4085 1132
Size: 1522 1431 3774
Fragments arranged by size:
3774 1522 1431
SspI AAT'ATT
Cuts at: 5 52 4651 5
Size: 47 4599 2081
Fragments arranged by size:
4599 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6727
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2316 2990 3291 3453 3531
Size: 552 939 84 228 674 301 162 78
Cuts at: 3531 3579 3645 4166 513
Size: 48 66 521 3074
Fragments arranged by size:
3074 939 674 552 521 301 228 162
84 78 66 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3019 4754 4771 4924 5109 5554 325
Size: 1255 1439 1735 17 153 185 445 1498
Fragments arranged by size:
1735 1498 1439 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4973 6685 96
Size: 274 80 33 51 1443 2996 1712 138
Fragments arranged by size:
2996 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6687 6719 6687
Size: 32 6695
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6727
XbaI T'CTAG_A
Cuts at: 4063 4063
Size: 6727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2440 2086
Size: 354 6373
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6727
XmaI C'CCGG_G
Cuts at: 1870 3759 4052 1870
Size: 1889 293 4545
Fragments arranged by size:
4545 1889 293
XmnI GAAnn'nnTTC
Cuts at: 4856 4856
Size: 6727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4535 276
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII FseI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI