Restriction map for
pFN21AB3831
(Circular) (Six-base) MAPSORT of: pFN21AB3831.seq Check: 5020 from: 1 to: 6808
pFN21AB3831 6808 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2398 2703 2398
Size: 305 6503
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2223 4618 278
Size: 53 83 186 936 687 2395 2468
Fragments arranged by size:
2468 2395 936 687 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4151 1937
Size: 2214 4594
Acc65I G'GTAC_C
Cuts at: 4129 4129
Size: 6808
AclI AA'CG_TT
Cuts at: 1563 4935 5308 1563
Size: 3372 373 3063
Fragments arranged by size:
3372 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2365 4871 6255 6523 1632
Size: 485 248 2506 1384 268 1917
Fragments arranged by size:
2506 1917 1384 485 268 248
AfeI AGC'GCT
Cuts at: 2945 2945
Size: 6808
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 2784 5707 6684 1203
Size: 1581 2923 977 1327
Fragments arranged by size:
2923 1581 1327 977
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 2224 5537 2224
Size: 3313 3495
AleI CACnn'nnGTG
Cuts at: 1166 2915 3269 1166
Size: 1749 354 4705
Fragments arranged by size:
4705 1749 354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3554 3950 1184
Size: 2370 396 4042
Fragments arranged by size:
4042 2370 396
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 6776
AlwNI CAG_nnn'CTG
Cuts at: 2161 2635 3133 3227 3901 4551 6123 2161
Size: 474 498 94 674 650 1572 2846
Fragments arranged by size:
2846 1572 674 650 498 474 94
ApaI G_GGCC'C
Cuts at: 2730 3364 3788 3922 4004 2730
Size: 634 424 134 82 5534
Fragments arranged by size:
5534 634 424 134 82
ApaLI G'TGCA_C
Cuts at: 4043 4865 6021 4043
Size: 822 1156 4830
Fragments arranged by size:
4830 1156 822
ApoI r'AATT_y
Cuts at: 1493 4117 4343 4495 6604 1493
Size: 2624 226 152 2109 1697
Fragments arranged by size:
2624 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2953 3992 4004 4133 1870
Size: 73 133 877 1039 12 129 4545
Fragments arranged by size:
4545 1039 877 133 129 73 12
BamHI G'GATC_C
Cuts at: 2709 4138 4505 2709
Size: 1429 367 5012
Fragments arranged by size:
5012 1429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2254 2351 2718 2768
Size: 325 217 627 260 207 97 367 50
Cuts at: 2768 2872 3295 3431 3811 4129 5584 618
Size: 104 423 136 380 318 1455 1842
Fragments arranged by size:
1842 1455 627 423 380 367 325 318
260 217 207 136 104 97 50
BanII G_rGCy'C
Cuts at: 1391 2427 2730 2844 3009 3364 3407 3609 3737
Size: 1036 303 114 165 355 43 202 128
Cuts at: 3737 3788 3922 4004 4072 4127 1391
Size: 51 134 82 68 55 4072
Fragments arranged by size:
4072 1036 355 303 202 165 134 128
114 82 68 55 51 43
BbeI G_GCGC'C
Cuts at: 2772 2772
Size: 6808
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3224 928
Size: 971 1325 4512
Fragments arranged by size:
4512 1325 971
BbvCI CC'TCA_GC
Cuts at: 4017 4017
Size: 6808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2630 2664 4999 5033 1903
Size: 34 693 34 2335 34 3678
Fragments arranged by size:
3678 2335 693 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4700 5910 4700
Size: 1210 5598
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6808
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 2777 3056
Size: 107 122 71 631 605 985 120 279
Cuts at: 3056 3200 3510 3542 5419 136
Size: 144 310 32 1877 1525
Fragments arranged by size:
1877 1525 985 631 605 310 279 144
122 120 107 71 32
BglII A'GATC_T
Cuts at: 6803 6803
Size: 6808
BlpI GC'TnA_GC
Cuts at: 2753 3500 4225 2753
Size: 747 725 5336
Fragments arranged by size:
5336 747 725
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2730 3364 3788 3816 3922 4004 4047
Size: 564 376 634 424 28 106 82 43
Cuts at: 4047 4869 6025 1790
Size: 822 1156 2573
Fragments arranged by size:
2573 1156 822 634 564 424 376 106
82 43 28
BmgBI CAC'GTC
Cuts at: 1318 2794 1318
Size: 1476 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4539 5497 442
Size: 416 53 83 399 24 3122 958 1753
Fragments arranged by size:
3122 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2831 3944 1056
Size: 1775 1113 3920
Fragments arranged by size:
3920 1775 1113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3751 4007 3751
Size: 256 6552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 4106 5468 1239
Size: 1172 1695 1362 2579
Fragments arranged by size:
2579 1695 1362 1172
Bpu10I CC'TnA_GC
Cuts at: 2019 3603 4017 2019
Size: 1584 414 4810
Fragments arranged by size:
4810 1584 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4933 5798 6096 4933
Size: 865 298 5645
Fragments arranged by size:
5645 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5471 882
Size: 617 3972 2219
Fragments arranged by size:
3972 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4599 493
Size: 711 3395 2702
Fragments arranged by size:
3395 2702 711
BsaBI GATnn'nnATC
Cuts at: 4101 6802 4101
Size: 2701 4107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2220 2592 2769 3483
Size: 53 83 186 936 687 372 177 714
Cuts at: 3483 4615 4997 5632 275
Size: 1132 382 635 1451
Fragments arranged by size:
1451 1132 936 714 687 635 382 372
186 177 83 53
BsaWI w'CCGG_w
Cuts at: 5241 5639 5913 6060 6471 5241
Size: 398 274 147 411 5578
Fragments arranged by size:
5578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4016 4046 1178
Size: 30 165 30 2613 30 3940
Fragments arranged by size:
3940 2613 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3996 4034 4037 1186
Size: 2810 38 3 3957
Fragments arranged by size:
3957 2810 38 3
BseYI C'CCAG_C
Cuts at: 2068 2602 2698 6011 6507 2068
Size: 534 96 3313 496 2369
Fragments arranged by size:
3313 2369 534 496 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 2146 2174 4076 1163
Size: 573 398 12 28 1902 3895
Fragments arranged by size:
3895 1902 573 398 28 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 3825 4258 5019 6047 1540
Size: 275 186 1513 311 433 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 433 311 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 2844 3890 4047 4869 4954 6025 2427
Size: 417 1046 157 822 85 1071 3210
Fragments arranged by size:
3210 1071 1046 822 417 157 85
BsmI GAATG_Cn'
Cuts at: 4319 4412 4319
Size: 93 6715
BsmBI CGTCTCn'nnnn_
Cuts at: 3734 3734
Size: 6808
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 2427 2730 2844 3009 3364 3407
Size: 399 564 73 303 114 165 355 43
Cuts at: 3407 3609 3737 3788 3816 3890 3922 4004 4047
Size: 202 128 51 28 74 32 82 43
Cuts at: 4047 4072 4127 4869 4954 6025 1391
Size: 25 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 355 303 202
165 128 114 85 82 74 73 55
51 43 43 32 28 25
BspEI T'CCGG_A
Cuts at: 6471 6471
Size: 6808
BspHI T'CATG_A
Cuts at: 4697 6427 4697
Size: 1730 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2398 2703 3909 4163 844
Size: 1325 229 305 1206 254 3489
Fragments arranged by size:
3489 1325 1206 305 254 229
BsrBI CCG'CTC
Cuts at: 1394 1823 4695 1394
Size: 429 2872 3507
Fragments arranged by size:
3507 2872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2276 5303 5477 65
Size: 1183 1028 3027 174 1396
Fragments arranged by size:
3027 1396 1183 1028 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2050 2435 3509 3824 3878 5452
Size: 110 234 6 385 1074 315 54 1574
Cuts at: 5452 5639 1700
Size: 187 2869
Fragments arranged by size:
2869 1574 1074 385 315 234 187 110
54 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6808
BssSI C'ACGA_G
Cuts at: 2122 2915 3053 4065 4868 5880 2122
Size: 793 138 1012 803 1012 3050
Fragments arranged by size:
3050 1012 1012 803 793 138
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 2811 3901 794
Size: 1367 650 1090 3701
Fragments arranged by size:
3701 1367 1090 650
BstBI TT'CG_AA
Cuts at: 5612 5612
Size: 6808
BstEII G'GTnAC_C
Cuts at: 1184 2797 1184
Size: 1613 5195
BstXI CCAn_nnnn'nTGG
Cuts at: 3079 3211 3079
Size: 132 6676
BstYI r'GATC_y
Cuts at: 1972 2709 4138 4505 4890 4907 6359 6445 6457
Size: 737 1429 367 385 17 1452 86 12
Cuts at: 6457 6803 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1429 737 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2867 2994 2867
Size: 127 6681
BtgI C'CryG_G
Cuts at: 513 1065 2004 2198 2370 2652 2714 2883 2976
Size: 552 939 194 172 282 62 169 93
Cuts at: 2976 3039 3267 3386 3435 513
Size: 63 228 119 49 3886
Fragments arranged by size:
3886 939 552 282 228 194 172 169
119 93 63 62 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2251 487
Size: 683 56 1025 5044
Fragments arranged by size:
5044 1025 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 2633 3175 4324 5118 5138 2134
Size: 499 542 1149 794 20 3804
Fragments arranged by size:
3804 1149 794 542 499 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6808
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6773
DraI TTT'AAA
Cuts at: 4112 4112
Size: 6808
DraIII CAC_nnn'GTG
Cuts at: 2811 3838 2811
Size: 1027 5781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3244 3419 5815 809
Size: 507 1928 175 2396 1802
Fragments arranged by size:
2396 1928 1802 507 175
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2433 2907
Size: 54 1425 50 275 18 453 150 474
Cuts at: 2907 3201 3438 3507 3511 3822 4255 5144 6773
Size: 294 237 69 4 311 433 889 1629
Cuts at: 6773 8
Size: 43
Fragments arranged by size:
1629 1425 889 474 453 433 311 294
275 237 150 69 54 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 3511 3822 4255 1537
Size: 275 1699 311 433 4090
Fragments arranged by size:
4090 1699 433 311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4738 1353
Size: 3385 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2605 3098 3412 5395 5769 5915
Size: 1064 138 1016 493 314 1983 374 146
Cuts at: 5915 387
Size: 1280
Fragments arranged by size:
1983 1280 1064 1016 493 374 314 146
138
EcoICRI GAG'CTC
Cuts at: 2425 2842 2425
Size: 417 6391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2365 2411 4106 4871 5468 6255
Size: 393 485 248 46 1695 765 597 787
Cuts at: 6255 6523 1239
Size: 268 1524
Fragments arranged by size:
1695 1524 787 765 597 485 393 268
248 46
EcoNI CCTnn'n_nnAGG
Cuts at: 3368 3995 3368
Size: 627 6181
EcoO109I rG'GnC_Cy
Cuts at: 2158 2581 2658 2726 2998 3360 3361 3379 3592
Size: 423 77 68 272 362 1 18 213
Cuts at: 3592 3761 3778 3898 3918 3919 4000 4001 2158
Size: 169 17 120 20 1 81 1 4965
Fragments arranged by size:
4965 423 362 272 213 169 120 81
77 68 20 18 17 1 1 1
EcoRI G'AATT_C
Cuts at: 4117 4495 4117
Size: 378 6430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3679 3679
Size: 6808
FseI GG_CCGG'CC
Cuts at: 3513 3513
Size: 6808
FspI TGC'GCA
Cuts at: 3368 5314 3368
Size: 1946 4862
HaeII r_GCGC'y
Cuts at: 1678 2068 2150 2772 2947 3195 3264 3293 5955
Size: 390 82 622 175 248 69 29 2662
Cuts at: 5955 1678
Size: 2531
Fragments arranged by size:
2662 2531 622 390 248 175 82 69
29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 5449 5481 5523
Size: 32 135 32 638 32 3207 32 42
Cuts at: 5523 5555 1373
Size: 32 2626
Fragments arranged by size:
3207 2626 638 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4152 4398 669
Size: 988 281 2214 246 3079
Fragments arranged by size:
3079 2214 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6808
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KasI G'GCGC_C
Cuts at: 2768 2768
Size: 6808
KpnI G_GTAC'C
Cuts at: 4133 4133
Size: 6808
MfeI C'AATT_G
Cuts at: 4407 4407
Size: 6808
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2080 5922 6106 1305
Size: 775 3842 184 2007
Fragments arranged by size:
3842 2007 775 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2909 3203 10
Size: 54 1425 343 1077 294 3615
Fragments arranged by size:
3615 1425 1077 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2561 2903 2915 3269 3446 4766
Size: 648 1086 309 342 12 354 177 1320
Cuts at: 4766 5125 5284 518
Size: 359 159 2042
Fragments arranged by size:
2042 1320 1086 648 359 354 342 309
177 159 12
MspA1I CmG'CkG
Cuts at: 1606 2118 2200 2431 2716 2878 3244 3301 3388
Size: 512 82 231 285 162 366 57 87
Cuts at: 3388 3437 3640 3821 3859 4030 4224 4901 6049
Size: 49 203 181 38 171 194 677 1148
Cuts at: 6049 6294 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 512 366 285 245 231
203 194 181 171 162 87 82 57
49 38
NaeI GCC'GGC
Cuts at: 2437 3511 3826 2437
Size: 1074 315 5419
Fragments arranged by size:
5419 1074 315
NarI GG'CG_CC
Cuts at: 2769 2769
Size: 6808
NcoI C'CATG_G
Cuts at: 513 1065 2004 2652 513
Size: 552 939 648 4669
Fragments arranged by size:
4669 939 648 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6808
NgoMIV G'CCGG_C
Cuts at: 2435 3509 3824 2435
Size: 1074 315 5419
Fragments arranged by size:
5419 1074 315
NheI G'CTAG_C
Cuts at: 1052 2827 3940 1052
Size: 1775 1113 3920
Fragments arranged by size:
3920 1775 1113
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NspI r_CATG'y
Cuts at: 1132 1229 2366 4166 5711 1132
Size: 97 1137 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 1137 97
PasI CC'CwG_GG
Cuts at: 1348 3769 3922 1348
Size: 2421 153 4234
Fragments arranged by size:
4234 2421 153
PciI A'CATG_T
Cuts at: 5707 5707
Size: 6808
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2109 2178 3838 1108
Size: 1001 69 1660 4078
Fragments arranged by size:
4078 1660 1001 69
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4877 4909 6390 6422 4016
Size: 32 829 32 1481 32 4402
Fragments arranged by size:
4402 1481 829 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2581 2998 3379 3778 3898 2158
Size: 423 417 381 399 120 5068
Fragments arranged by size:
5068 423 417 399 381 120
PshAI GACnn'nnGTC
Cuts at: 1613 3746 1613
Size: 2133 4675
PsiI TTA'TAA
Cuts at: 4378 4378
Size: 6808
PspOMI G'GGCC_C
Cuts at: 2726 3360 3784 3918 4000 2726
Size: 634 424 134 82 5534
Fragments arranged by size:
5534 634 424 134 82
PspXI vC'TCGA_Gb
Cuts at: 2953 2953
Size: 6808
PstI C_TGCA'G
Cuts at: 830 2031 2295 2466 3672 3897 3953 4160 830
Size: 1201 264 171 1206 225 56 207 3478
Fragments arranged by size:
3478 1206 1201 264 225 207 171 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
PvuII CAG'CTG
Cuts at: 2118 2431 3244 4030 2118
Size: 313 813 786 4896
Fragments arranged by size:
4896 813 786 313
RsrII CG'GwC_CG
Cuts at: 2053 2053
Size: 6808
SacI G_AGCT'C
Cuts at: 2427 2844 2427
Size: 417 6391
SacII CC_GC'GG
Cuts at: 2201 2717 3389 3438 2201
Size: 516 672 49 5571
Fragments arranged by size:
5571 672 516 49
SalI G'TCGA_C
Cuts at: 1936 4150 1936
Size: 2214 4594
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 5056 6768 5056
Size: 1712 5096
SexAI A'CCwGG_T
Cuts at: 2577 2577
Size: 6808
SfcI C'TryA_G
Cuts at: 826 1045 2027 2291 2302 2462 3314 3668 3679
Size: 219 982 264 11 160 852 354 11
Cuts at: 3679 3893 3949 4156 5291 5972 6163 826
Size: 214 56 207 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 852 681 354 264 219
214 207 191 160 56 11 11
SfoI GGC'GCC
Cuts at: 2770 2770
Size: 6808
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 2078 4006 4135 1872
Size: 206 1928 129 4545
Fragments arranged by size:
4545 1928 206 129
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2953 4912 5813 6075 820
Size: 197 926 1010 1959 901 262 1553
Fragments arranged by size:
1959 1553 1010 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4599 493
Size: 4106 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 4166 1132
Size: 3034 3774
SspI AAT'ATT
Cuts at: 5 52 4732 5
Size: 47 4680 2081
Fragments arranged by size:
4680 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6808
StyI C'CwwG_G
Cuts at: 513 1065 2004 2652 4247 513
Size: 552 939 648 1595 3074
Fragments arranged by size:
3074 1595 939 648 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 4835 4852 5005 5190 5635 325
Size: 1255 1953 1302 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1302 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3642 5054 6766
Size: 274 80 33 51 1443 1665 1412 1712
Cuts at: 6766 96
Size: 138
Fragments arranged by size:
1712 1665 1443 1412 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3288 3320 3689 3721 6768 6800 3288
Size: 32 369 32 3047 32 3296
Fragments arranged by size:
3296 3047 369 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2795 1316
Size: 1479 5329
XbaI T'CTAG_A
Cuts at: 4144 4144
Size: 6808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2890 2945 3196 3448 3805 2890
Size: 55 251 252 357 5893
Fragments arranged by size:
5893 357 252 251 55
XhoI C'TCGA_G
Cuts at: 1943 2953 1943
Size: 1010 5798
XmaI C'CCGG_G
Cuts at: 1870 2076 4004 4133 1870
Size: 206 1928 129 4545
Fragments arranged by size:
4545 1928 206 129
XmnI GAAnn'nnTTC
Cuts at: 4937 4937
Size: 6808
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2221 4616 276
Size: 53 83 186 936 687 2395 2468
Fragments arranged by size:
2468 2395 936 687 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PfoI PmlI PsrI SanDI SapI
SfiI SrfI SwaI