Restriction map for
pFN21AB3827
(Circular) (Six-base) MAPSORT of: pFN21AB3827.seq Check: 7259 from: 1 to: 8209
pFN21AB3827 8209 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4603 4603
Size: 8209
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6019 278
Size: 53 83 186 936 4483 2468
Fragments arranged by size:
4483 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3342 5552 1937
Size: 1405 2210 4594
Fragments arranged by size:
4594 2210 1405
Acc65I G'GTAC_C
Cuts at: 5530 5530
Size: 8209
AclI AA'CG_TT
Cuts at: 1563 6336 6709 1563
Size: 4773 373 3063
Fragments arranged by size:
4773 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 3702 3943 4816 4827 5243 6272 7656
Size: 1979 91 241 873 11 416 1029 1384
Cuts at: 7656 7924 1632
Size: 268 1917
Fragments arranged by size:
1979 1917 1384 1029 873 416 268 241
91 11
AfeI AGC'GCT
Cuts at: 2262 2262
Size: 8209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8012
AflIII A'CryG_T
Cuts at: 1203 3177 3306 3332 4573 4657 7108 8085 1203
Size: 1974 129 26 1241 84 2451 977 1327
Fragments arranged by size:
2451 1974 1327 1241 977 129 84 26
AgeI A'CCGG_T
Cuts at: 7040 7040
Size: 8209
AhdI GACnn_n'nnGTC
Cuts at: 4451 6938 4451
Size: 2487 5722
AleI CACnn'nnGTG
Cuts at: 1166 4772 1166
Size: 3606 4603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8209
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5094 5126 5094
Size: 32 8177
AlwNI CAG_nnn'CTG
Cuts at: 3504 3695 4231 5952 7524 3504
Size: 191 536 1721 1572 4189
Fragments arranged by size:
4189 1721 1572 536 191
ApaLI G'TGCA_C
Cuts at: 3551 4682 6266 7422 3551
Size: 1131 1584 1156 4338
Fragments arranged by size:
4338 1584 1156 1131
ApoI r'AATT_y
Cuts at: 1493 2906 4928 5518 5744 5896 8005 1493
Size: 1413 2022 590 226 152 2109 1697
Fragments arranged by size:
2109 2022 1697 1413 590 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6763 160
Size: 872 5731 1606
Fragments arranged by size:
5731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8209
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2249 2280 2388 2430 5534 1870
Size: 73 146 160 31 108 42 3104 4545
Fragments arranged by size:
4545 3104 160 146 108 73 42 31
AvrII C'CTAG_G
Cuts at: 2162 2162
Size: 8209
BamHI G'GATC_C
Cuts at: 5125 5539 5906 5125
Size: 414 367 7428
Fragments arranged by size:
7428 414 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 3520 4760 5530 6985
Size: 325 217 627 231 1502 1240 770 1455
Cuts at: 6985 618
Size: 1842
Fragments arranged by size:
1842 1502 1455 1240 770 627 325 231
217
BanII G_rGCy'C
Cuts at: 1391 2073 2200 2281 2387 2621 5528 1391
Size: 682 127 81 106 234 2907 4072
Fragments arranged by size:
4072 2907 682 234 127 106 81
BbeI G_GCGC'C
Cuts at: 2022 3524 2022
Size: 1502 6707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 3479 928
Size: 971 963 617 5658
Fragments arranged by size:
5658 971 963 617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4720 4754 6400 6434 1903
Size: 34 2783 34 1646 34 3678
Fragments arranged by size:
3678 2783 1646 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6101 7311 6101
Size: 1210 6999
BclI T'GATC_A
Cuts at: 1548 2772 4806 1548
Size: 1224 2034 4951
Fragments arranged by size:
4951 2034 1224
BfrBI ATG'CAT
Cuts at: 3197 3244 3197
Size: 47 8162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3390 4758 6820
Size: 107 122 71 631 605 1718 1368 2062
Cuts at: 6820 136
Size: 1525
Fragments arranged by size:
2062 1718 1525 1368 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3744 3857 8204 3744
Size: 113 4347 3749
Fragments arranged by size:
4347 3749 113
BlpI GC'TnA_GC
Cuts at: 2045 5626 2045
Size: 3581 4628
Bme1580I G_kGCm'C
Cuts at: 1790 2288 3555 4382 4686 4763 5497 6270 7426
Size: 498 1267 827 304 77 734 773 1156
Cuts at: 7426 1790
Size: 2573
Fragments arranged by size:
2573 1267 1156 827 773 734 498 304
77
BmgBI CAC'GTC
Cuts at: 1318 4576 1318
Size: 3258 4951
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2298 4314 5940
Size: 416 53 83 399 24 881 2016 1626
Cuts at: 5940 6898 442
Size: 958 1753
Fragments arranged by size:
2016 1753 1626 958 881 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 3757 3942 5154 5183 6869 1239
Size: 1052 1466 185 1212 29 1686 2579
Fragments arranged by size:
2579 1686 1466 1212 1052 185 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3026 6334 7199 7497 3026
Size: 3308 865 298 3738
Fragments arranged by size:
3738 3308 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3601 4879 4941 6872 882
Size: 617 2102 1278 62 1931 2219
Fragments arranged by size:
2219 2102 1931 1278 617 62
BsaAI yAC'GTr
Cuts at: 493 1204 3178 3309 3335 4681 6000 493
Size: 711 1974 131 26 1346 1319 2702
Fragments arranged by size:
2702 1974 1346 1319 711 131 26
BsaBI GATnn'nnATC
Cuts at: 3264 8203 3264
Size: 4939 3270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 2094 3521 6016
Size: 53 83 186 936 486 75 1427 2495
Cuts at: 6016 6398 7033 275
Size: 382 635 1451
Fragments arranged by size:
2495 1451 1427 936 635 486 382 186
83 75 53
BsaWI w'CCGG_w
Cuts at: 2463 4932 6642 7040 7314 7461 7872 2463
Size: 2469 1710 398 274 147 411 2800
Fragments arranged by size:
2800 2469 1710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3780 3810 5223 5253 1178
Size: 30 165 30 2377 30 1413 30 4134
Fragments arranged by size:
4134 2377 1413 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2341 2486 5011 1186
Size: 859 296 145 2525 4384
Fragments arranged by size:
4384 2525 859 296 145
BseYI C'CCAG_C
Cuts at: 2132 4748 4880 5489 7412 7908 2132
Size: 2616 132 609 1923 496 2433
Fragments arranged by size:
2616 2433 1923 609 496 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2295 4037 4156 4631 4889 1163
Size: 573 559 1742 119 475 258 4483
Fragments arranged by size:
4483 1742 573 559 475 258 119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 5011 5659 6420 7448 1540
Size: 275 186 322 2688 648 761 1028 2301
Fragments arranged by size:
2688 2301 1028 761 648 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2621 3555 3762 4680 4686 5079 6270 6355 7426
Size: 934 207 918 6 393 1191 85 1071
Cuts at: 7426 2621
Size: 3404
Fragments arranged by size:
3404 1191 1071 934 918 393 207 85
6
BsmI GAATG_Cn'
Cuts at: 3057 3199 4891 5720 5813 3057
Size: 142 1692 829 93 5453
Fragments arranged by size:
5453 1692 829 142 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2323 2323
Size: 8209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2200 2281 2288 2387 2621 3555
Size: 399 283 127 81 7 99 234 934
Cuts at: 3555 3762 4382 4680 4686 4763 5079 5497 5528
Size: 207 620 298 6 77 316 418 31
Cuts at: 5528 6270 6355 7426 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 934 742 620 418 399 316
298 283 234 207 127 99 85 81
77 31 7 6
BspEI T'CCGG_A
Cuts at: 2463 7872 2463
Size: 5409 2800
BspHI T'CATG_A
Cuts at: 3399 3939 6098 7828 3399
Size: 540 2159 1730 3780
Fragments arranged by size:
3780 2159 1730 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4603 4861 5564 844
Size: 3759 258 703 3489
Fragments arranged by size:
3759 3489 703 258
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 2337 2342 2437 3969 4117 6096
Size: 429 452 62 5 95 1532 148 1979
Cuts at: 6096 1394
Size: 3507
Fragments arranged by size:
3507 1979 1532 452 429 148 95 62
5
BsrDI GCAATG_nn'
Cuts at: 65 1248 3624 4225 6704 6878 65
Size: 1183 2376 601 2479 174 1396
Fragments arranged by size:
2479 2376 1396 1183 601 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2060 2808 4234 6853 7040 1700
Size: 110 211 39 748 1426 2619 187 2869
Fragments arranged by size:
2869 2619 1426 748 211 187 110 39
BsrGI T'GTAC_A
Cuts at: 96 3062 96
Size: 2966 5243
BssHII G'CGCG_C
Cuts at: 1925 2049 1925
Size: 124 8085
BssSI C'ACGA_G
Cuts at: 6269 7281 6269
Size: 1012 7197
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3559 5501 794
Size: 2765 1942 3502
Fragments arranged by size:
3502 2765 1942
BstBI TT'CG_AA
Cuts at: 7013 7013
Size: 8209
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8209
BstXI CCAn_nnnn'nTGG
Cuts at: 2546 3019 4756 2546
Size: 473 1737 5999
Fragments arranged by size:
5999 1737 473
BstYI r'GATC_y
Cuts at: 1972 3532 3744 3857 5125 5303 5483 5539 5906
Size: 1560 212 113 1268 178 180 56 367
Cuts at: 5906 6291 6308 7760 7846 7858 8204 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1560 1452 1268 385 367 346 212
180 178 113 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 3387 3387
Size: 8209
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 2532 4394 4554 513
Size: 552 939 294 234 1862 160 4168
Fragments arranged by size:
4168 1862 939 552 294 234 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4533 4897 487
Size: 683 56 3307 364 3799
Fragments arranged by size:
3799 3307 683 364 56
BtsI GCAGTG_nn'
Cuts at: 3553 4359 4743 5094 5355 5725 6519 6539 3553
Size: 806 384 351 261 370 794 20 5223
Fragments arranged by size:
5223 806 794 384 370 351 261 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8174
DraI TTT'AAA
Cuts at: 5371 5513 5371
Size: 142 8067
DraIII CAC_nnn'GTG
Cuts at: 3338 3338
Size: 8209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4643 7216 809
Size: 507 3327 2573 1802
Fragments arranged by size:
3327 2573 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2186 2320 2548
Size: 54 1425 50 275 18 356 134 228
Cuts at: 2548 3164 4222 5656 6545 8174 8
Size: 616 1058 1434 889 1629 43
Fragments arranged by size:
1629 1434 1425 1058 889 616 356 275
228 134 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2320 5656 1537
Size: 275 508 3336 4090
Fragments arranged by size:
4090 3336 508 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3721 3750 3921 4023 4470 4698 4944 6139
Size: 2368 29 171 102 447 228 246 1195
Cuts at: 6139 1353
Size: 3423
Fragments arranged by size:
3423 2368 1195 447 246 228 171 102
29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5451 6796 7170 7316 387
Size: 1064 138 3862 1345 374 146 1280
Fragments arranged by size:
3862 1345 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2619 2619
Size: 8209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 3611 3702 3757 3942 3943 4816
Size: 393 659 1320 91 55 185 1 873
Cuts at: 4816 4827 5154 5183 5243 6272 6869 7656 7924
Size: 11 327 29 60 1029 597 787 268
Cuts at: 7924 1239
Size: 1524
Fragments arranged by size:
1524 1320 1029 873 787 659 597 393
327 268 185 91 60 55 29 11
1
EcoNI CCTnn'n_nnAGG
Cuts at: 3090 3187 3413 3090
Size: 97 226 7886
Fragments arranged by size:
7886 226 97
EcoO109I rG'GnC_Cy
Cuts at: 2440 3383 3544 2440
Size: 943 161 7105
Fragments arranged by size:
7105 943 161
EcoRI G'AATT_C
Cuts at: 4928 5518 5896 4928
Size: 590 378 7241
Fragments arranged by size:
7241 590 378
EcoRV GAT'ATC
Cuts at: 5172 5172
Size: 8209
FspI TGC'GCA
Cuts at: 6715 6715
Size: 8209
HaeII r_GCGC'y
Cuts at: 1678 2022 2264 3524 4644 7356 1678
Size: 344 242 1260 1120 2712 2531
Fragments arranged by size:
2712 2531 1260 1120 344 242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3095 3127 3986 4018 4437
Size: 32 135 32 1523 32 859 32 419
Cuts at: 4437 4469 5156 5188 5223 5229 5255 5261 6850
Size: 32 687 32 35 6 26 6 1589
Cuts at: 6850 6882 6924 6956 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1589 1523 859 687 419 135 42
35 32 32 32 32 32 32 32
32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2634 5151 5553 5799 669
Size: 988 281 696 2517 402 246 3079
Fragments arranged by size:
3079 2517 988 696 402 281 246
HindIII A'AGCT_T
Cuts at: 748 2830 3212 748
Size: 2082 382 5745
Fragments arranged by size:
5745 2082 382
HpaI GTT'AAC
Cuts at: 5799 5799
Size: 8209
KasI G'GCGC_C
Cuts at: 2018 3520 2018
Size: 1502 6707
KpnI G_GTAC'C
Cuts at: 5534 5534
Size: 8209
MfeI C'AATT_G
Cuts at: 5505 5808 5505
Size: 303 7906
MluI A'CGCG_T
Cuts at: 8085 8085
Size: 8209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2360 5315 7323 7507 1305
Size: 1055 2955 2008 184 2007
Fragments arranged by size:
2955 2008 2007 1055 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 4224 10
Size: 54 1425 343 718 1674 3995
Fragments arranged by size:
3995 1674 1425 718 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 3704 3944 4218 4460 4505 4772
Size: 648 2490 48 240 274 242 45 267
Cuts at: 4772 4865 5066 5315 6167 6526 6685 518
Size: 93 201 249 852 359 159 2042
Fragments arranged by size:
2490 2042 852 648 359 274 267 249
242 240 201 159 93 48 45
MspA1I CmG'CkG
Cuts at: 1606 3148 3281 3982 4051 4396 4436 4492 4880
Size: 1542 133 701 69 345 40 56 388
Cuts at: 4880 5132 5267 5625 6302 7450 7695 1606
Size: 252 135 358 677 1148 245 2120
Fragments arranged by size:
2120 1542 1148 701 677 388 358 345
252 245 135 133 69 56 40
NaeI GCC'GGC
Cuts at: 2023 2062 4236 2023
Size: 39 2174 5996
Fragments arranged by size:
5996 2174 39
NarI GG'CG_CC
Cuts at: 2019 3521 2019
Size: 1502 6707
NcoI C'CATG_G
Cuts at: 513 1065 2004 2298 2532 4554 513
Size: 552 939 294 234 2022 4168
Fragments arranged by size:
4168 2022 939 552 294 234
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8209
NgoMIV G'CCGG_C
Cuts at: 2021 2060 4234 2021
Size: 39 2174 5996
Fragments arranged by size:
5996 2174 39
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8209
NotI GC'GGCC_GC
Cuts at: 5656 5656
Size: 8209
NsiI A_TGCA'T
Cuts at: 3199 3246 3199
Size: 47 8162
NspI r_CATG'y
Cuts at: 1132 1229 3244 3248 3709 4074 4661 5567 7112
Size: 97 2015 4 461 365 587 906 1545
Cuts at: 7112 1132
Size: 2229
Fragments arranged by size:
2229 2015 1545 906 587 461 365 97
4
PasI CC'CwG_GG
Cuts at: 1348 4382 1348
Size: 3034 5175
PciI A'CATG_T
Cuts at: 4657 7108 4657
Size: 2451 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 1108
Size: 1425 6784
PmeI GTTT'AAAC
Cuts at: 5513 5513
Size: 8209
PmlI CAC'GTG
Cuts at: 3335 4681 3335
Size: 1346 6863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6278 6310 7791 7823 6278
Size: 32 1481 32 6664
Fragments arranged by size:
6664 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 3544 2440
Size: 1104 7105
PshAI GACnn'nnGTC
Cuts at: 1613 4623 5007 1613
Size: 3010 384 4815
Fragments arranged by size:
4815 3010 384
PsiI TTA'TAA
Cuts at: 5779 5779
Size: 8209
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3661 3693 3661
Size: 32 8177
PstI C_TGCA'G
Cuts at: 830 3519 5561 830
Size: 2689 2042 3478
Fragments arranged by size:
3478 2689 2042
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8209
PvuII CAG'CTG
Cuts at: 3148 3982 4051 4880 5132 3148
Size: 834 69 829 252 6225
Fragments arranged by size:
6225 834 829 252 69
SacI G_AGCT'C
Cuts at: 2621 2621
Size: 8209
SacII CC_GC'GG
Cuts at: 4397 4397
Size: 8209
SalI G'TCGA_C
Cuts at: 1936 5551 1936
Size: 3615 4594
SanDI GG'GwC_CC
Cuts at: 2440 3544 2440
Size: 1104 7105
SapI GCTCTTCn'nnn_
Cuts at: 3750 3750
Size: 8209
SbfI CC_TGCA'GG
Cuts at: 5561 5561
Size: 8209
ScaI AGT'ACT
Cuts at: 4303 6457 8169 4303
Size: 2154 1712 4343
Fragments arranged by size:
4343 2154 1712
SexAI A'CCwGG_T
Cuts at: 5416 5416
Size: 8209
SfcI C'TryA_G
Cuts at: 826 1045 3515 4036 5557 6692 7373 7564 826
Size: 219 2470 521 1521 1135 681 191 1471
Fragments arranged by size:
2470 1521 1471 1135 681 521 219 191
SfoI GGC'GCC
Cuts at: 2020 3522 2020
Size: 1502 6707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8209
SgrAI Cr'CCGG_yG
Cuts at: 1700 2021 2060 1700
Size: 321 39 7849
Fragments arranged by size:
7849 321 39
SmaI CCC'GGG
Cuts at: 1872 2091 2251 2390 5536 1872
Size: 219 160 139 3146 4545
Fragments arranged by size:
4545 3146 219 160 139
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3005 6313 7214 7476 820
Size: 197 926 1062 3308 901 262 1553
Fragments arranged by size:
3308 1553 1062 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6000 493
Size: 5507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8209
SphI G_CATG'C
Cuts at: 1132 5567 1132
Size: 4435 3774
SspI AAT'ATT
Cuts at: 5 52 6133 5
Size: 47 6081 2081
Fragments arranged by size:
6081 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8209
StyI C'CwwG_G
Cuts at: 513 1065 2004 2162 2298 2532 3234 3357 4043
Size: 552 939 158 136 234 702 123 686
Cuts at: 4043 4554 4671 4812 5648 513
Size: 511 117 141 836 3074
Fragments arranged by size:
3074 939 836 702 686 552 511 234
158 141 136 123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2271 3902 4997 6236 6253 6406 6591
Size: 1255 691 1631 1095 1239 17 153 185
Cuts at: 6591 7036 325
Size: 445 1498
Fragments arranged by size:
1631 1498 1255 1239 1095 691 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3062 4301 4356
Size: 274 80 33 51 1443 1085 1239 55
Cuts at: 4356 4650 5209 6455 8167 96
Size: 294 559 1246 1712 138
Fragments arranged by size:
1712 1443 1246 1239 1085 559 294 274
138 80 55 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3871 3903 5032 5064 8169 8201 3871
Size: 32 1129 32 3105 32 3879
Fragments arranged by size:
3879 3105 1129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8209
XbaI T'CTAG_A
Cuts at: 5545 5545
Size: 8209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4511 4511
Size: 8209
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8209
XmaI C'CCGG_G
Cuts at: 1870 2089 2249 2388 5534 1870
Size: 219 160 139 3146 4545
Fragments arranged by size:
4545 3146 219 160 139
XmnI GAAnn'nnTTC
Cuts at: 3476 6338 3476
Size: 2862 5347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6017 276
Size: 53 83 186 936 4483 2468
Fragments arranged by size:
4483 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbvCI BplI Bpu10I BsiWI BstZ17I
FalI FseI FspAI NruI PacI PfoI PspOMI PspXI
RsrII SfiI SrfI SwaI