Restriction map for
pFN21AB3824
(Circular) (Six-base) MAPSORT of: pFN21AB3824.seq Check: 8517 from: 1 to: 7813
pFN21AB3824 7813 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3412 3938 4118 3412
Size: 526 180 7107
Fragments arranged by size:
7107 526 180
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5623 278
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5156 1937
Size: 3219 4594
Acc65I G'GTAC_C
Cuts at: 5134 5134
Size: 7813
AclI AA'CG_TT
Cuts at: 1563 5940 6313 1563
Size: 4377 373 3063
Fragments arranged by size:
4377 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2589 3683 3780 4450 5876 7260 7528 1632
Size: 957 1094 97 670 1426 1384 268 1917
Fragments arranged by size:
1917 1426 1384 1094 957 670 268 97
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7616
AflIII A'CryG_T
Cuts at: 1203 2196 3045 6712 7689 1203
Size: 993 849 3667 977 1327
Fragments arranged by size:
3667 1327 993 977 849
AgeI A'CCGG_T
Cuts at: 3246 6644 3246
Size: 3398 4415
AhdI GACnn_n'nnGTC
Cuts at: 2805 6542 2805
Size: 3737 4076
AleI CACnn'nnGTG
Cuts at: 1166 3678 4467 4797 1166
Size: 2512 789 330 4182
Fragments arranged by size:
4182 2512 789 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2869 1184
Size: 1685 6128
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 7781
AlwNI CAG_nnn'CTG
Cuts at: 2154 2631 5556 7128 2154
Size: 477 2925 1572 2839
Fragments arranged by size:
2925 2839 1572 477
ApaLI G'TGCA_C
Cuts at: 4105 5870 7026 4105
Size: 1765 1156 4892
Fragments arranged by size:
4892 1765 1156
ApoI r'AATT_y
Cuts at: 1493 5122 5348 5500 7609 1493
Size: 3629 226 152 2109 1697
Fragments arranged by size:
3629 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3453 6367 160
Size: 872 2421 2914 1606
Fragments arranged by size:
2914 2421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
AvaI C'yCGr_G
Cuts at: 1870 1943 2100 2158 5138 1870
Size: 73 157 58 2980 4545
Fragments arranged by size:
4545 2980 157 73 58
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4303 4336 4303
Size: 33 7780
BamHI G'GATC_C
Cuts at: 2921 5143 5510 2921
Size: 2222 367 5224
Fragments arranged by size:
5224 2222 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2096 2231 3879 3937
Size: 325 217 627 259 50 135 1648 58
Cuts at: 3937 5134 6589 618
Size: 1197 1455 1842
Fragments arranged by size:
1842 1648 1455 1197 627 325 259 217
135 58 50
BanII G_rGCy'C
Cuts at: 1391 2148 3714 4023 4590 5132 1391
Size: 757 1566 309 567 542 4072
Fragments arranged by size:
4072 1566 757 567 542 309
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3264 4440 4673 928
Size: 971 1365 1176 233 4068
Fragments arranged by size:
4068 1365 1176 971 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2576 2610 6004 6038 1903
Size: 34 639 34 3394 34 3678
Fragments arranged by size:
3678 3394 639 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3198 4390 4896 5705 6915 3198
Size: 1192 506 809 1210 4096
Fragments arranged by size:
4096 1210 1192 809 506
BclI T'GATC_A
Cuts at: 1548 3924 1548
Size: 2376 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3087 4536 6424
Size: 107 122 71 631 605 1415 1449 1888
Cuts at: 6424 136
Size: 1525
Fragments arranged by size:
1888 1525 1449 1415 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3588 3726 7808 3588
Size: 138 4082 3593
Fragments arranged by size:
4082 3593 138
BlpI GC'TnA_GC
Cuts at: 5230 5230
Size: 7813
Bme1580I G_kGCm'C
Cuts at: 1790 2049 3150 3824 3922 4109 4151 5874 7030
Size: 259 1101 674 98 187 42 1723 1156
Cuts at: 7030 1790
Size: 2573
Fragments arranged by size:
2573 1723 1156 1101 674 259 187 98
42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3106 3161 5544
Size: 416 53 83 399 24 1689 55 2383
Cuts at: 5544 6502 442
Size: 958 1753
Fragments arranged by size:
2383 1753 1689 958 416 399 83 55
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7813
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 7813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4071 4715 4748 6473 1239
Size: 2832 644 33 1725 2579
Fragments arranged by size:
2832 2579 1725 644 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2814 4420 5938 6803 7101 2814
Size: 1606 1518 865 298 3526
Fragments arranged by size:
3526 1606 1518 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 3237 6476 882
Size: 617 563 1175 3239 2219
Fragments arranged by size:
3239 2219 1175 617 563
BsaAI yAC'GTr
Cuts at: 493 1204 2199 3145 5604 493
Size: 711 995 946 2459 2702
Fragments arranged by size:
2702 2459 995 946 711
BsaBI GATnn'nnATC
Cuts at: 3843 7807 3843
Size: 3964 3849
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2178 5620 6002 6637
Size: 53 83 186 936 645 3442 382 635
Cuts at: 6637 275
Size: 1451
Fragments arranged by size:
3442 1451 936 645 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3246 6246 6644 6918 7065 7476 3246
Size: 3000 398 274 147 411 3583
Fragments arranged by size:
3583 3000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4076 4106 1178
Size: 30 165 30 2673 30 4885
Fragments arranged by size:
4885 2673 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2589 3875 4068 4505 4695 1186
Size: 970 433 1286 193 437 190 4304
Fragments arranged by size:
4304 1286 970 437 433 193 190
BseYI C'CCAG_C
Cuts at: 2065 2624 3800 7016 7512 2065
Size: 559 1176 3216 496 2366
Fragments arranged by size:
3216 2366 1176 559 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 3250 3313 3412 3523 3640 3799
Size: 573 456 1058 63 99 111 117 159
Cuts at: 3799 4011 4028 4498 4775 1163
Size: 212 17 470 277 4201
Fragments arranged by size:
4201 1058 573 470 456 277 212 159
117 111 99 63 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2260 3312 4557 5263 6024 7052
Size: 275 186 259 1052 1245 706 761 1028
Cuts at: 7052 1540
Size: 2301
Fragments arranged by size:
2301 1245 1052 1028 761 706 275 259
186
BsiHKAI G_wGCw'C
Cuts at: 3714 4023 4109 5874 5959 7030 3714
Size: 309 86 1765 85 1071 4497
Fragments arranged by size:
4497 1765 1071 309 86 85
BsmI GAATG_Cn'
Cuts at: 2786 3893 4871 5022 5324 5417 2786
Size: 1107 978 151 302 93 5182
Fragments arranged by size:
5182 1107 978 302 151 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2148 3150 3714 3824 3922 4023
Size: 399 259 99 1002 564 110 98 101
Cuts at: 4023 4109 4151 4590 5132 5874 5959 7030 1391
Size: 86 42 439 542 742 85 1071 2174
Fragments arranged by size:
2174 1071 1002 742 564 542 439 399
259 110 101 99 98 86 85 42
BspEI T'CCGG_A
Cuts at: 7476 7476
Size: 7813
BspHI T'CATG_A
Cuts at: 5702 7432 5702
Size: 1730 6083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2261 2453 3335 3412 3938 4118 5168 844
Size: 1417 192 882 77 526 180 1050 3489
Fragments arranged by size:
3489 1417 1050 882 526 192 180 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 4495 5700 1394
Size: 429 467 2205 1205 3507
Fragments arranged by size:
3507 2205 1205 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2386 4821 6308 6482 65
Size: 1183 1138 2435 1487 174 1396
Fragments arranged by size:
2435 1487 1396 1183 1138 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 3246 6457 6644 1700
Size: 110 226 1210 3211 187 2869
Fragments arranged by size:
3211 2869 1210 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 4205 96
Size: 4109 3704
BssHII G'CGCG_C
Cuts at: 1925 2087 1925
Size: 162 7651
BssSI C'ACGA_G
Cuts at: 2943 5873 6885 2943
Size: 2930 1012 3871
Fragments arranged by size:
3871 2930 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2667 3273 4521 794
Size: 1873 606 1248 4086
Fragments arranged by size:
4086 1873 1248 606
BstBI TT'CG_AA
Cuts at: 2440 3052 6617 2440
Size: 612 3565 3636
Fragments arranged by size:
3636 3565 612
BstEII G'GTnAC_C
Cuts at: 1184 4757 1184
Size: 3573 4240
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2363 4049 4469 4597 2011
Size: 352 1686 420 128 5227
Fragments arranged by size:
5227 1686 420 352 128
BstYI r'GATC_y
Cuts at: 1972 2921 3178 3588 3726 5143 5510 5895 5912
Size: 949 257 410 138 1417 367 385 17
Cuts at: 5912 7364 7450 7462 7808 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1417 949 410 385 367 346
257 138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2707 2707
Size: 7813
BtgI C'CryG_G
Cuts at: 513 1065 3238 3834 513
Size: 552 2173 596 4492
Fragments arranged by size:
4492 2173 596 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2964 487
Size: 683 56 1738 5336
Fragments arranged by size:
5336 1738 683 56
BtsI GCAGTG_nn'
Cuts at: 4519 4907 5329 6123 6143 4519
Size: 388 422 794 20 6189
Fragments arranged by size:
6189 794 422 388 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7813
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3639 3674 4434 4469 529
Size: 35 3075 35 760 35 3873
Fragments arranged by size:
3873 3075 760 35 35 35
DraI TTT'AAA
Cuts at: 2371 2496 5117 2371
Size: 125 2621 5067
Fragments arranged by size:
5067 2621 125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6820 809
Size: 507 5504 1802
Fragments arranged by size:
5504 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 5260 6149
Size: 54 1425 50 275 18 204 3226 889
Cuts at: 6149 7778 8
Size: 1629 43
Fragments arranged by size:
3226 1629 1425 889 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5260 1537
Size: 275 3448 4090
Fragments arranged by size:
4090 3448 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 5743 1353
Size: 1152 3238 3423
Fragments arranged by size:
3423 3238 1152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 6400 6774 6920 387
Size: 1064 138 681 4130 374 146 1280
Fragments arranged by size:
4130 1280 1064 681 374 146 138
EcoICRI GAG'CTC
Cuts at: 3712 4021 3712
Size: 309 7504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2589 3683 3780 4071 4450 4715 4748
Size: 393 957 1094 97 291 379 265 33
Cuts at: 4748 5876 6473 7260 7528 1239
Size: 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 1094 957 787 597 393 379
291 268 265 97 33
EcoO109I rG'GnC_Cy
Cuts at: 4501 4501
Size: 7813
EcoRI G'AATT_C
Cuts at: 5122 5500 5122
Size: 378 7435
EcoRV GAT'ATC
Cuts at: 4732 4732
Size: 7813
FspI TGC'GCA
Cuts at: 2660 6319 2660
Size: 3659 4154
HaeII r_GCGC'y
Cuts at: 1678 2108 6960 1678
Size: 430 4852 2531
Fragments arranged by size:
4852 2531 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2791 2823 2906 2938 3615
Size: 32 135 32 1219 32 83 32 677
Cuts at: 3615 3647 4660 4692 6454 6486 6528 6560 1373
Size: 32 1013 32 1762 32 42 32 2626
Fragments arranged by size:
2626 1762 1219 1013 677 135 83 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3286 4690 5157 5403 669
Size: 988 281 1348 1404 467 246 3079
Fragments arranged by size:
3079 1404 1348 988 467 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7813
HpaI GTT'AAC
Cuts at: 5403 5403
Size: 7813
KpnI G_GTAC'C
Cuts at: 5138 5138
Size: 7813
MfeI C'AATT_G
Cuts at: 5412 5412
Size: 7813
MluI A'CGCG_T
Cuts at: 7689 7689
Size: 7813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3464 6927 7111 1305
Size: 2159 3463 184 2007
Fragments arranged by size:
3463 2159 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5991
Fragments arranged by size:
5991 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 3406 3678 3855 4467 4595 4797
Size: 648 1527 713 272 177 612 128 202
Cuts at: 4797 5771 6130 6289 518
Size: 974 359 159 2042
Fragments arranged by size:
2042 1527 974 713 648 612 359 272
202 177 159 128
MspA1I CmG'CkG
Cuts at: 1606 2538 2631 3206 3800 4119 4156 4295 4326
Size: 932 93 575 594 319 37 139 31
Cuts at: 4326 4512 4563 5229 5906 7054 7299 1606
Size: 186 51 666 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 677 666 594 575 319
245 186 139 93 51 37 31
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 7813
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7813
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 7813
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7813
NotI GC'GGCC_GC
Cuts at: 5260 5260
Size: 7813
NspI r_CATG'y
Cuts at: 1132 1229 3049 3411 5068 5171 6716 1132
Size: 97 1820 362 1657 103 1545 2229
Fragments arranged by size:
2229 1820 1657 1545 362 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7813
PciI A'CATG_T
Cuts at: 3045 6712 3045
Size: 3667 4146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5110 1108
Size: 4002 3811
PfoI T'CCnGG_A
Cuts at: 4301 4301
Size: 7813
PmeI GTTT'AAAC
Cuts at: 5117 5117
Size: 7813
PmlI CAC'GTG
Cuts at: 2199 2199
Size: 7813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5882 5914 7395 7427 4076
Size: 32 1774 32 1481 32 4462
Fragments arranged by size:
4462 1774 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4501 4501
Size: 7813
PshAI GACnn'nnGTC
Cuts at: 1613 3063 3687 1613
Size: 1450 624 5739
Fragments arranged by size:
5739 1450 624
PsiI TTA'TAA
Cuts at: 4865 5383 4865
Size: 518 7295
PstI C_TGCA'G
Cuts at: 830 2450 2771 2848 4536 5165 830
Size: 1620 321 77 1688 629 3478
Fragments arranged by size:
3478 1688 1620 629 321 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7813
PvuII CAG'CTG
Cuts at: 3800 4295 4326 4512 4563 3800
Size: 495 31 186 51 7050
Fragments arranged by size:
7050 495 186 51 31
SacI G_AGCT'C
Cuts at: 3714 4023 3714
Size: 309 7504
SalI G'TCGA_C
Cuts at: 1936 5155 1936
Size: 3219 4594
SbfI CC_TGCA'GG
Cuts at: 4536 5165 4536
Size: 629 7184
ScaI AGT'ACT
Cuts at: 4027 6061 7773 4027
Size: 2034 1712 4067
Fragments arranged by size:
4067 2034 1712
SfcI C'TryA_G
Cuts at: 826 1045 2446 2767 2844 4532 5059 5084 5161
Size: 219 1401 321 77 1688 527 25 77
Cuts at: 5161 6296 6977 7168 826
Size: 1135 681 191 1471
Fragments arranged by size:
1688 1471 1401 1135 681 527 321 219
191 77 77 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7813
SmaI CCC'GGG
Cuts at: 1872 2102 2160 5140 1872
Size: 230 58 2980 4545
Fragments arranged by size:
4545 2980 230 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2829 4399 5917 6818 7080 820
Size: 197 926 886 1570 1518 901 262 1553
Fragments arranged by size:
1570 1553 1518 926 901 886 262 197
SnaBI TAC'GTA
Cuts at: 493 5604 493
Size: 5111 2702
SpeI A'CTAG_T
Cuts at: 152 2692 152
Size: 2540 5273
SphI G_CATG'C
Cuts at: 1132 5068 5171 1132
Size: 3936 103 3774
Fragments arranged by size:
3936 3774 103
SspI AAT'ATT
Cuts at: 5 52 3032 5737 5
Size: 47 2980 2705 2081
Fragments arranged by size:
2980 2705 2081 47
StuI AGG'CCT
Cuts at: 1517 3788 4478 1517
Size: 2271 690 4852
Fragments arranged by size:
4852 2271 690
StyI C'CwwG_G
Cuts at: 513 1065 2529 3082 3605 3735 5252 513
Size: 552 1464 553 523 130 1517 3074
Fragments arranged by size:
3074 1517 1464 553 552 523 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3823 3867 4196 4532 4809 5840 5857
Size: 1255 2243 44 329 336 277 1031 17
Cuts at: 5857 6010 6195 6640 325
Size: 153 185 445 1498
Fragments arranged by size:
2243 1498 1255 1031 445 336 329 277
185 153 44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2858 4025
Size: 274 80 33 51 1443 30 851 1167
Cuts at: 4025 4097 4205 6059 7771 96
Size: 72 108 1854 1712 138
Fragments arranged by size:
1854 1712 1443 1167 851 274 138 108
80 72 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3382 3414 7773 7805 3382
Size: 32 4359 32 3390
Fragments arranged by size:
4359 3390 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7813
XbaI T'CTAG_A
Cuts at: 5149 5149
Size: 7813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4914 4996 2011
Size: 2903 82 4828
Fragments arranged by size:
4828 2903 82
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7813
XmaI C'CCGG_G
Cuts at: 1870 2100 2158 5138 1870
Size: 230 58 2980 4545
Fragments arranged by size:
4545 2980 230 58
XmnI GAAnn'nnTTC
Cuts at: 5942 5942
Size: 7813
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5621 276
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI
KasI NarI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI