Restriction map for
pFN21AB3821
(Circular) (Six-base) MAPSORT of: pFN21AB3821.seq Check: 364 from: 1 to: 5974
pFN21AB3821 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 5421 5689 1632
Size: 2405 1384 268 1917
Fragments arranged by size:
2405 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2085 3271 3717 5289 2085
Size: 1186 446 1572 2770
Fragments arranged by size:
2770 1572 1186 446
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2219 4528 160
Size: 872 1187 2309 1606
Fragments arranged by size:
2309 1606 1187 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BamHI G'GATC_C
Cuts at: 2390 3304 3671 2390
Size: 914 367 4693
Fragments arranged by size:
4693 914 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 3295 4750 618
Size: 325 217 627 539 969 1455 1842
Fragments arranged by size:
1842 1455 969 627 539 325 217
BanII G_rGCy'C
Cuts at: 1391 3293 1391
Size: 1902 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4165 4199 1903
Size: 34 50 34 2144 34 3678
Fragments arranged by size:
3678 2144 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3866 5076 2586
Size: 1280 1210 3484
Fragments arranged by size:
3484 1280 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2107 2897 4634 1239
Size: 868 790 1737 2579
Fragments arranged by size:
2579 1737 868 790
Bpu10I CC'TnA_GC
Cuts at: 3206 3206
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2163 2774 3048 4099 4964 5262 2163
Size: 611 274 1051 865 298 2875
Fragments arranged by size:
2875 1051 865 611 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2286 4637 882
Size: 617 787 2351 2219
Fragments arranged by size:
2351 2219 787 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 3781 4163 4798
Size: 53 83 186 936 519 1729 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1729 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2080 2110 2576 2606 2682
Size: 30 165 30 677 30 466 30 76
Cuts at: 2682 2712 1178
Size: 30 4440
Fragments arranged by size:
4440 677 466 165 76 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 1186
Size: 1504 4470
BseYI C'CCAG_C
Cuts at: 2154 5177 5673 2154
Size: 3023 496 2455
Fragments arranged by size:
3023 2455 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 1163
Size: 573 406 4995
Fragments arranged by size:
4995 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 3424 4185 5213 1540
Size: 275 186 135 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186 135
BsiHKAI G_wGCw'C
Cuts at: 2672 4035 4120 5191 2672
Size: 1363 85 1071 3455
Fragments arranged by size:
3455 1363 1071 85
BsmI GAATG_Cn'
Cuts at: 3160 3485 3578 3160
Size: 325 93 5556
Fragments arranged by size:
5556 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2672 3293 4035 4120 5191 1391
Size: 399 882 621 742 85 1071 2174
Fragments arranged by size:
2174 1071 882 742 621 399 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 2298 3863 5593 2298
Size: 1565 1730 2679
Fragments arranged by size:
2679 1730 1565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3217 3861 1394
Size: 429 1394 644 3507
Fragments arranged by size:
3507 1394 644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 2041 1925
Size: 116 5858
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2109 2109
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2390 3304 3671 4056 4073 5525 5611 5623
Size: 418 914 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 914 418 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2835 2835
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2046 2102 513
Size: 552 981 56 4385
Fragments arranged by size:
4385 981 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 487
Size: 683 56 1094 4141
Fragments arranged by size:
4141 1094 683 56
BtsI GCAGTG_nn'
Cuts at: 2795 3490 4284 4304 2795
Size: 695 794 20 4465
Fragments arranged by size:
4465 794 695 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3064 3099 529
Size: 35 2500 35 3404
Fragments arranged by size:
3404 2500 35 35
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3147 3421
Size: 54 1425 50 275 18 303 1014 274
Cuts at: 3421 4310 5939 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1014 889 303 275 274 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2133 3421 1537
Size: 275 321 1288 4090
Fragments arranged by size:
4090 1288 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 2285 2523 2753 3097 3904 1353
Size: 836 96 238 230 344 807 3423
Fragments arranged by size:
3423 836 807 344 238 230 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2403 4561 4935 5081 387
Size: 1064 138 814 2158 374 146 1280
Fragments arranged by size:
2158 1280 1064 814 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2897 4037 4634 5421 5689 1239
Size: 393 475 790 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 790 787 597 475 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2237 2939 3142 2237
Size: 702 203 5069
Fragments arranged by size:
5069 702 203
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2850 5121 1678
Size: 1172 2271 2531
Fragments arranged by size:
2531 2271 1172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 2382 2414 2438
Size: 32 135 32 508 32 270 32 24
Cuts at: 2438 2470 3233 3265 4615 4647 4689 4721 1373
Size: 32 763 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 763 508 270 135 42 32
32 32 32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2815 3318 3564 669
Size: 988 281 877 503 246 3079
Fragments arranged by size:
3079 988 877 503 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 2523 3573 2523
Size: 1050 4924
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2107 3932 4291 4450 518
Size: 648 941 1825 359 159 2042
Fragments arranged by size:
2042 1825 941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2071 2085 2126 2132 2589 3199 3271
Size: 442 23 14 41 6 457 610 72
Cuts at: 3271 3390 4067 5215 5460 1606
Size: 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 610 457 442 245 119
72 41 23 14 6
NcoI C'CATG_G
Cuts at: 513 1065 2102 513
Size: 552 1037 4385
Fragments arranged by size:
4385 1037 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2162 3332 4877 1132
Size: 97 933 1170 1545 2229
Fragments arranged by size:
2229 1545 1170 933 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 5158
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2085 3271 1108
Size: 977 1186 3811
Fragments arranged by size:
3811 1186 977
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2939 2939
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2131 3239 3326 830
Size: 1301 1108 87 3478
Fragments arranged by size:
3478 1301 1108 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2085 2126 2589 3199 3271 2085
Size: 41 463 610 72 4788
Fragments arranged by size:
4788 610 463 72 41
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 3097 3097
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 2882 3059 4222 5934 2882
Size: 177 1163 1712 2922
Fragments arranged by size:
2922 1712 1163 177
SfcI C'TryA_G
Cuts at: 826 1045 2127 3235 3322 4457 5138 5329 826
Size: 219 1082 1108 87 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 1082 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 2753 3063 4078 4979 5241
Size: 197 926 235 575 310 1015 901 262
Cuts at: 5241 820
Size: 1553
Fragments arranged by size:
1553 1015 926 901 575 310 262 235
197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 2162 3332 1132
Size: 1030 1170 3774
Fragments arranged by size:
3774 1170 1030
SspI AAT'ATT
Cuts at: 5 52 2217 3898 5
Size: 47 2165 1681 2081
Fragments arranged by size:
2165 2081 1681 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2102 2913 3075 3413 513
Size: 552 1037 811 162 338 3074
Fragments arranged by size:
3074 1037 811 552 338 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 4001 4018 4171 4356 4801 325
Size: 1255 724 1697 17 153 185 445 1498
Fragments arranged by size:
1697 1498 1255 724 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2583 2880 3057
Size: 274 80 33 51 1443 606 297 177
Cuts at: 3057 4220 5932 96
Size: 1163 1712 138
Fragments arranged by size:
1712 1443 1163 606 297 274 177 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2104 2136 2560 2592 2682 2714 5934 5966 2104
Size: 32 424 32 90 32 3220 32 2112
Fragments arranged by size:
3220 2112 424 90 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3268 3268
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI