Restriction map for
pFN21AB3799
(Circular) (Six-base) MAPSORT of: pFN21AB3799.seq Check: 726 from: 1 to: 6160
pFN21AB3799 6160 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3165 3503 1937
Size: 1228 338 4594
Fragments arranged by size:
4594 1228 338
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 2069 4287 4660 1563
Size: 506 2218 373 3063
Fragments arranged by size:
3063 2218 506 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3209 4223 5607 5875 1632
Size: 1577 1014 1384 268 1917
Fragments arranged by size:
1917 1577 1384 1014 268
AfeI AGC'GCT
Cuts at: 2151 2198 2785 2151
Size: 47 587 5526
Fragments arranged by size:
5526 587 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 2052 2457 3294 5059 6036 1203
Size: 849 405 837 1765 977 1327
Fragments arranged by size:
1765 1327 977 849 837 405
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 2655 2834 1166
Size: 1489 179 4492
Fragments arranged by size:
4492 1489 179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2129 2429 1184
Size: 945 300 4915
Fragments arranged by size:
4915 945 300
AlwNI CAG_nnn'CTG
Cuts at: 2116 2167 2188 3903 5475 2116
Size: 51 21 1715 1572 2801
Fragments arranged by size:
2801 1715 1572 51 21
ApaI G_GGCC'C
Cuts at: 2208 2311 2320 2208
Size: 103 9 6048
Fragments arranged by size:
6048 103 9
ApaLI G'TGCA_C
Cuts at: 3302 3363 4217 5373 3302
Size: 61 854 1156 4089
Fragments arranged by size:
4089 1156 854 61
ApoI r'AATT_y
Cuts at: 1493 3469 3695 3847 5956 1493
Size: 1976 226 152 2109 1697
Fragments arranged by size:
2109 1976 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 3105 3485 1870
Size: 73 361 801 380 4545
Fragments arranged by size:
4545 801 380 361 73
BamHI G'GATC_C
Cuts at: 3109 3490 3857 3109
Size: 381 367 5412
Fragments arranged by size:
5412 381 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2139 2325 2413 2611 3481
Size: 325 217 627 352 186 88 198 870
Cuts at: 3481 4936 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 870 627 352 325 217 198
186 88
BanII G_rGCy'C
Cuts at: 1391 2208 2311 2320 2903 3062 3218 3479 1391
Size: 817 103 9 583 159 156 261 4072
Fragments arranged by size:
4072 817 583 261 159 156 103 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2980 928
Size: 971 1081 4108
Fragments arranged by size:
4108 1081 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2230 2264 2459 2493 3418 3452 4351
Size: 34 293 34 195 34 925 34 899
Cuts at: 4351 4385 1903
Size: 34 3678
Fragments arranged by size:
3678 925 899 293 195 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2315 4771 136
Size: 107 122 71 631 605 643 2456 1525
Fragments arranged by size:
2456 1525 643 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2208 2311 2320 2328 2418 3306 3367
Size: 352 66 103 9 8 90 888 61
Cuts at: 3367 4221 5377 1790
Size: 854 1156 2573
Fragments arranged by size:
2573 1156 888 854 352 103 90 66
61 9 8
BmgBI CAC'GTC
Cuts at: 1318 2421 2460 1318
Size: 1103 39 5018
Fragments arranged by size:
5018 1103 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3891 4849 442
Size: 416 53 83 399 24 2474 958 1753
Fragments arranged by size:
2474 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2887 3397 3463 4820 1239
Size: 1648 510 66 1357 2579
Fragments arranged by size:
2579 1648 1357 510 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4285 5150 5448 4285
Size: 865 298 4997
Fragments arranged by size:
4997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4823 882
Size: 617 3324 2219
Fragments arranged by size:
3324 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2515 3951 493
Size: 711 1311 1436 2702
Fragments arranged by size:
2702 1436 1311 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3420 3967 4349 4984
Size: 53 83 186 936 1887 547 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1887 1451 936 635 547 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5935
Fragments arranged by size:
5935 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6160
BseYI C'CCAG_C
Cuts at: 2135 2146 2188 5363 5859 2135
Size: 11 42 3175 496 2436
Fragments arranged by size:
3175 2436 496 42 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2785 1163
Size: 573 1049 4538
Fragments arranged by size:
4538 1049 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2230 3218 3306 3367 4221 4306 5377 2230
Size: 988 88 61 854 85 1071 3013
Fragments arranged by size:
3013 1071 988 854 88 85 61
BsmI GAATG_Cn'
Cuts at: 2131 3671 3764 2131
Size: 1540 93 4527
Fragments arranged by size:
4527 1540 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2208 2230 2311 2320 2328 2418
Size: 399 352 66 22 81 9 8 90
Cuts at: 2418 2903 3062 3218 3306 3367 3479 4221 4306
Size: 485 159 156 88 61 112 742 85
Cuts at: 4306 5377 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 352 159 156
112 90 88 85 81 66 61 22
9 8
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 2865 4049 5779 2865
Size: 1184 1730 3246
Fragments arranged by size:
3246 1730 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3515 844
Size: 2671 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3322 4047 1394
Size: 429 1499 725 3507
Fragments arranged by size:
3507 1499 725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 2952 4804 4991 1700
Size: 110 290 852 1852 187 2869
Fragments arranged by size:
2869 1852 852 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 2229 2699 4220 5232 2229
Size: 470 1521 1012 3157
Fragments arranged by size:
3157 1521 1012 470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2857 794
Size: 2063 4097
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstXI CCAn_nnnn'nTGG
Cuts at: 2657 2713 3040 3430 2657
Size: 56 327 390 5387
Fragments arranged by size:
5387 390 327 56
BstYI r'GATC_y
Cuts at: 1972 2766 3109 3490 3857 4242 4259 5711 5797
Size: 794 343 381 367 385 17 1452 86
Cuts at: 5797 5809 6155 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 794 385 381 367 346 343
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2168 2168
Size: 6160
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2320 2445 2656 2977 513
Size: 552 939 6 310 125 211 321 3696
Fragments arranged by size:
3696 939 552 321 310 211 125 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 2230 2989 3116 3292 3676 4470 4490 2040
Size: 190 759 127 176 384 794 20 3710
Fragments arranged by size:
3710 794 759 384 190 176 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DraIII CAC_nnn'GTG
Cuts at: 2857 2857
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2448 2950
Size: 54 1425 50 275 18 177 441 502
Cuts at: 2950 3009 3327 3607 4496 6125 8
Size: 59 318 280 889 1629 43
Fragments arranged by size:
1629 1425 889 502 441 318 280 275
177 59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3185 4090 1353
Size: 1832 905 3423
Fragments arranged by size:
3423 1832 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 3248 4747 5121 5267 387
Size: 1064 138 498 1161 1499 374 146 1280
Fragments arranged by size:
1499 1280 1161 1064 498 374 146 138
EcoICRI GAG'CTC
Cuts at: 3216 3216
Size: 6160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2887 3209 3397 3463 4223 4820 5607
Size: 393 1255 322 188 66 760 597 787
Cuts at: 5607 5875 1239
Size: 268 1524
Fragments arranged by size:
1524 1255 787 760 597 393 322 268
188 66
EcoO109I rG'GnC_Cy
Cuts at: 2205 2308 2316 2397 3177 3267 2205
Size: 103 8 81 780 90 5098
Fragments arranged by size:
5098 780 103 90 81 8
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 6160
FspI TGC'GCA
Cuts at: 2729 3230 4666 2729
Size: 501 1436 4223
Fragments arranged by size:
4223 1436 501
FspAI rTGC'GCAy
Cuts at: 2729 2729
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2153 2200 2787 5307 1678
Size: 475 47 587 2520 2531
Fragments arranged by size:
2531 2520 587 475 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3205 3237 4801 4833 4875
Size: 32 135 32 1633 32 1564 32 42
Cuts at: 4875 4907 1373
Size: 32 2626
Fragments arranged by size:
2626 1633 1564 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2818 3343 3504 3750 669
Size: 988 281 880 525 161 246 3079
Fragments arranged by size:
3079 988 880 525 281 246 161
HindIII A'AGCT_T
Cuts at: 748 3196 748
Size: 2448 3712
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 2052 6036 2052
Size: 3984 2176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 5274 5458 1305
Size: 1386 2583 184 2007
Fragments arranged by size:
2583 2007 1386 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3011 10
Size: 54 1425 343 1179 3159
Fragments arranged by size:
3159 1425 1179 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2517 2655 2699 2834 3299 4118 4477
Size: 648 1351 138 44 135 465 819 359
Cuts at: 4477 4636 518
Size: 159 2042
Fragments arranged by size:
2042 1351 819 648 465 359 159 138
135 44
MspA1I CmG'CkG
Cuts at: 1606 2012 2113 2185 2359 2608 3187 3426 3576
Size: 406 101 72 174 249 579 239 150
Cuts at: 3576 4253 5401 5646 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 406 249 245 239
174 150 101 72
NaeI GCC'GGC
Cuts at: 2102 2954 2102
Size: 852 5308
NcoI C'CATG_G
Cuts at: 513 1065 2004 2445 513
Size: 552 939 441 4228
Fragments arranged by size:
4228 939 552 441
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 2100 2952 2100
Size: 852 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2494 2864 3298 3518 5063 1132
Size: 97 1265 370 434 220 1545 2229
Fragments arranged by size:
2229 1545 1265 434 370 220 97
PasI CC'CwG_GG
Cuts at: 1348 2311 1348
Size: 963 5197
PciI A'CATG_T
Cuts at: 3294 5059 3294
Size: 1765 4395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 1108
Size: 1407 4753
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PmlI CAC'GTG
Cuts at: 2515 2515
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3177 3177
Size: 6160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspOMI G'GGCC_C
Cuts at: 2204 2307 2316 2204
Size: 103 9 6048
Fragments arranged by size:
6048 103 9
PstI C_TGCA'G
Cuts at: 830 2364 3186 3354 3512 830
Size: 1534 822 168 158 3478
Fragments arranged by size:
3478 1534 822 168 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2113 2185 2359 2608 3187 2113
Size: 72 174 249 579 5086
Fragments arranged by size:
5086 579 249 174 72
SacI G_AGCT'C
Cuts at: 3218 3218
Size: 6160
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SanDI GG'GwC_CC
Cuts at: 3177 3177
Size: 6160
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2236 2360 3154 3182 3350 3508 4643
Size: 219 1191 124 794 28 168 158 1135
Cuts at: 4643 5324 5515 826
Size: 681 191 1471
Fragments arranged by size:
1471 1191 1135 794 681 219 191 168
158 124 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 2315 2315
Size: 6160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 2100 1700
Size: 400 5760
SmaI CCC'GGG
Cuts at: 1872 3107 3487 1872
Size: 1235 380 4545
Fragments arranged by size:
4545 1235 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4264 5165 5427 820
Size: 197 926 2321 901 262 1553
Fragments arranged by size:
2321 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 2445 2531 3330 3599 513
Size: 552 939 126 315 86 799 269 3074
Fragments arranged by size:
3074 939 799 552 315 269 126 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2571 2638 4187 4204 4357 4542 4987
Size: 1255 991 67 1549 17 153 185 445
Cuts at: 4987 325
Size: 1498
Fragments arranged by size:
1549 1498 1255 991 445 185 153 67
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4406 6118 96
Size: 274 80 33 51 1443 2429 1712 138
Fragments arranged by size:
2429 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6120 6152 6120
Size: 32 6128
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3040 3040
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3105 3485 1870
Size: 1235 380 4545
Fragments arranged by size:
4545 1235 380
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI KasI
NarI NruI NsiI PacI PfoI PspXI PsrI RsrII
SapI SexAI SfoI SrfI SwaI