Restriction map for
pFN21AB3796
(Circular) (Six-base) MAPSORT of: pFN21AB3796.seq Check: 4877 from: 1 to: 7285
pFN21AB3796 7285 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3512 3512
Size: 7285
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5095 278
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4628 1937
Size: 2691 4594
Acc65I G'GTAC_C
Cuts at: 3012 3641 4606 3012
Size: 629 965 5691
Fragments arranged by size:
5691 965 629
AclI AA'CG_TT
Cuts at: 1563 5412 5785 1563
Size: 3849 373 3063
Fragments arranged by size:
3849 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3251 3302 5348 6732 7000 1632
Size: 1619 51 2046 1384 268 1917
Fragments arranged by size:
2046 1917 1619 1384 268 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7088
AflIII A'CryG_T
Cuts at: 1203 2365 2405 3372 3714 6184 7161 1203
Size: 1162 40 967 342 2470 977 1327
Fragments arranged by size:
2470 1327 1162 977 967 342 40
AgeI A'CCGG_T
Cuts at: 6116 6116
Size: 7285
AhdI GACnn_n'nnGTC
Cuts at: 6014 6014
Size: 7285
AleI CACnn'nnGTG
Cuts at: 1166 3834 1166
Size: 2668 4617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3862 1184
Size: 2678 4607
AlwNI CAG_nnn'CTG
Cuts at: 2955 5028 6600 2955
Size: 2073 1572 3640
Fragments arranged by size:
3640 2073 1572
ApaLI G'TGCA_C
Cuts at: 2830 4338 5342 6498 2830
Size: 1508 1004 1156 3617
Fragments arranged by size:
3617 1508 1156 1004
ApoI r'AATT_y
Cuts at: 1493 2711 3993 4594 4820 4972 7081 1493
Size: 1218 1282 601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1282 1218 601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5839 160
Size: 872 4807 1606
Fragments arranged by size:
4807 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 4610 1870
Size: 73 145 2522 4545
Fragments arranged by size:
4545 2522 145 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2304 2337 2304
Size: 33 7252
BamHI G'GATC_C
Cuts at: 2163 4615 4982 2163
Size: 2452 367 4466
Fragments arranged by size:
4466 2452 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3012 3620 3641 3904 4516
Size: 325 217 627 1225 608 21 263 612
Cuts at: 4516 4606 6061 618
Size: 90 1455 1842
Fragments arranged by size:
1842 1455 1225 627 612 608 325 263
217 90 21
BanII G_rGCy'C
Cuts at: 1391 4333 4509 4604 1391
Size: 2942 176 95 4072
Fragments arranged by size:
4072 2942 176 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3276 4007 4058 4091 928
Size: 971 1377 731 51 33 4122
Fragments arranged by size:
4122 1377 971 731 51 33
BbvCI CC'TCA_GC
Cuts at: 4287 4287
Size: 7285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5476 5510 1903
Size: 34 3539 34 3678
Fragments arranged by size:
3678 3539 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5177 6387 5177
Size: 1210 6075
BclI T'GATC_A
Cuts at: 1548 2241 1548
Size: 693 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3902 5896 136
Size: 107 122 71 631 605 2230 1994 1525
Fragments arranged by size:
2230 1994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7280 7280
Size: 7285
BlpI GC'TnA_GC
Cuts at: 4702 4702
Size: 7285
Bme1580I G_kGCm'C
Cuts at: 1790 2834 3393 3907 4342 4557 5346 6502 1790
Size: 1044 559 514 435 215 789 1156 2573
Fragments arranged by size:
2573 1156 1044 789 559 514 435 215
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 3910 4288
Size: 416 53 83 399 24 745 1748 378
Cuts at: 4288 4512 5016 5974 442
Size: 224 504 958 1753
Fragments arranged by size:
1753 1748 958 745 504 416 399 378
224 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2743 3089 3295 5945 1239
Size: 1504 346 206 2650 2579
Fragments arranged by size:
2650 2579 1504 346 206
Bpu10I CC'TnA_GC
Cuts at: 4287 4287
Size: 7285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5410 6275 6573 5410
Size: 865 298 6122
Fragments arranged by size:
6122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 2420 3053 5948 882
Size: 617 774 147 633 2895 2219
Fragments arranged by size:
2895 2219 774 633 617 147
BsaAI yAC'GTr
Cuts at: 493 1204 2406 3715 4337 5076 493
Size: 711 1202 1309 622 739 2702
Fragments arranged by size:
2702 1309 1202 739 711 622
BsaBI GATnn'nnATC
Cuts at: 7279 7279
Size: 7285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5092 5474 6109 275
Size: 53 83 186 936 3559 382 635 1451
Fragments arranged by size:
3559 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5718 6116 6390 6537 6948 5718
Size: 398 274 147 411 6055
Fragments arranged by size:
6055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7060
Fragments arranged by size:
7060 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3179 1186
Size: 1993 5292
BseYI C'CCAG_C
Cuts at: 2497 2911 3364 6488 6984 2497
Size: 414 453 3124 496 2798
Fragments arranged by size:
3124 2798 496 453 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2907 3322 3325 3365 3792 4362 1163
Size: 573 1171 415 3 40 427 570 4086
Fragments arranged by size:
4086 1171 573 570 427 415 40 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4735 5496 6524 1540
Size: 275 186 2734 761 1028 2301
Fragments arranged by size:
2734 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2068 2834 4342 5346 5431 6502 2068
Size: 766 1508 1004 85 1071 2851
Fragments arranged by size:
2851 1508 1071 1004 766 85
BsmI GAATG_Cn'
Cuts at: 4796 4889 4796
Size: 93 7192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2834 3393 3907 4333 4342 4509
Size: 399 278 766 559 514 426 9 167
Cuts at: 4509 4557 4604 5346 5431 6502 1391
Size: 48 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 766 742 559 514 426 399
278 167 85 48 47 9
BspEI T'CCGG_A
Cuts at: 6948 6948
Size: 7285
BspHI T'CATG_A
Cuts at: 2820 2935 2994 5174 6904 2820
Size: 115 59 2180 1730 3201
Fragments arranged by size:
3201 2180 1730 115 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3108 3387 3493 3512 4537 4640 844
Size: 2264 279 106 19 1025 103 3489
Fragments arranged by size:
3489 2264 1025 279 106 103 19
BsrBI CCG'CTC
Cuts at: 1394 1823 5172 1394
Size: 429 3349 3507
Fragments arranged by size:
3507 3349 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 3217 3846 5780 5954 65
Size: 1183 1023 946 629 1934 174 1396
Fragments arranged by size:
1934 1396 1183 1023 946 629 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 3627 5929 6116 1700
Size: 110 250 1567 2302 187 2869
Fragments arranged by size:
2869 2302 1567 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7285
BssHII G'CGCG_C
Cuts at: 1925 2012 1925
Size: 87 7198
BssSI C'ACGA_G
Cuts at: 3443 3830 5345 6357 3443
Size: 387 1515 1012 4371
Fragments arranged by size:
4371 1515 1012 387
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7285
BstBI TT'CG_AA
Cuts at: 6089 6089
Size: 7285
BstEII G'GTnAC_C
Cuts at: 1184 4000 1184
Size: 2816 4469
BstXI CCAn_nnnn'nTGG
Cuts at: 2809 3137 4494 2809
Size: 328 1357 5600
Fragments arranged by size:
5600 1357 328
BstYI r'GATC_y
Cuts at: 1972 2163 3495 4438 4559 4615 4982 5367 5384
Size: 191 1332 943 121 56 367 385 17
Cuts at: 5384 6836 6922 6934 7280 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1332 943 385 367 346 191
121 86 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2359 3024 3850 4132 513
Size: 552 939 355 665 826 282 3666
Fragments arranged by size:
3666 939 826 665 552 355 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3741 487
Size: 683 56 2515 4031
Fragments arranged by size:
4031 2515 683 56
BtsI GCAGTG_nn'
Cuts at: 3358 3458 3851 3886 4244 4801 5595 5615 3358
Size: 100 393 35 358 557 794 20 5028
Fragments arranged by size:
5028 794 557 393 358 100 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7250
DraI TTT'AAA
Cuts at: 4589 4589
Size: 7285
DraIII CAC_nnn'GTG
Cuts at: 3768 4133 4552 3768
Size: 365 419 6501
Fragments arranged by size:
6501 419 365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6292 809
Size: 507 4976 1802
Fragments arranged by size:
4976 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 3629 4124
Size: 54 1425 50 275 18 228 1571 495
Cuts at: 4124 4162 4446 4476 4732 5621 7250 8
Size: 38 284 30 256 889 1629 43
Fragments arranged by size:
1629 1571 1425 889 495 284 275 256
228 54 50 43 38 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 4732 1537
Size: 275 2920 4090
Fragments arranged by size:
4090 2920 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2110 2168 2498 3551 5215 1353
Size: 757 58 330 1053 1664 3423
Fragments arranged by size:
3423 1664 1053 757 330 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4499 5872 6246 6392 387
Size: 1064 138 2910 1373 374 146 1280
Fragments arranged by size:
2910 1373 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2743 3089 3251 3295 3302 5348 5945
Size: 393 1111 346 162 44 7 2046 597
Cuts at: 5945 6732 7000 1239
Size: 787 268 1524
Fragments arranged by size:
2046 1524 1111 787 597 393 346 268
162 44 7
EcoNI CCTnn'n_nnAGG
Cuts at: 4477 4477
Size: 7285
EcoO109I rG'GnC_Cy
Cuts at: 2155 2689 3708 4398 4482 2155
Size: 534 1019 690 84 4958
Fragments arranged by size:
4958 1019 690 534 84
EcoRI G'AATT_C
Cuts at: 3993 4594 4972 3993
Size: 601 378 6306
Fragments arranged by size:
6306 601 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3580 3946 3580
Size: 366 6919
FspI TGC'GCA
Cuts at: 2766 3250 5791 2766
Size: 484 2541 4260
Fragments arranged by size:
4260 2541 484
FspAI rTGC'GCAy
Cuts at: 3250 3250
Size: 7285
HaeII r_GCGC'y
Cuts at: 1678 6432 1678
Size: 4754 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 2475 2507 2660
Size: 32 135 32 661 32 210 32 153
Cuts at: 2660 2692 2976 3008 3377 3409 5926 5958 6000
Size: 32 284 32 369 32 2517 32 42
Cuts at: 6000 6032 1373
Size: 32 2626
Fragments arranged by size:
2626 2517 661 369 284 210 153 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4252 4299 4629 4875 669
Size: 988 281 2314 47 330 246 3079
Fragments arranged by size:
3079 2314 988 330 281 246 47
HindIII A'AGCT_T
Cuts at: 748 2735 748
Size: 1987 5298
HpaI GTT'AAC
Cuts at: 4875 4875
Size: 7285
KpnI G_GTAC'C
Cuts at: 3016 3645 4610 3016
Size: 629 965 5691
Fragments arranged by size:
5691 965 629
MfeI C'AATT_G
Cuts at: 4884 4884
Size: 7285
MluI A'CGCG_T
Cuts at: 7161 7161
Size: 7285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4165 6399 6583 1305
Size: 2860 2234 184 2007
Fragments arranged by size:
2860 2234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4448 4478 10
Size: 54 1425 343 2616 30 2817
Fragments arranged by size:
2817 2616 1425 343 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 2760 2807 2825 3834 5243 5602
Size: 648 1198 396 47 18 1009 1409 359
Cuts at: 5602 5761 518
Size: 159 2042
Fragments arranged by size:
2042 1409 1198 1009 648 396 359 159
47 18
MspA1I CmG'CkG
Cuts at: 1606 2020 3280 3951 4701 5378 6526 6771 1606
Size: 414 1260 671 750 677 1148 245 2120
Fragments arranged by size:
2120 1260 1148 750 677 671 414 245
NaeI GCC'GGC
Cuts at: 3629 3629
Size: 7285
NcoI C'CATG_G
Cuts at: 513 1065 2004 2359 3024 513
Size: 552 939 355 665 4774
Fragments arranged by size:
4774 939 665 552 355
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7285
NgoMIV G'CCGG_C
Cuts at: 3627 3627
Size: 7285
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7285
NotI GC'GGCC_GC
Cuts at: 4732 4732
Size: 7285
NspI r_CATG'y
Cuts at: 1132 1229 2369 2830 3736 4643 6188 1132
Size: 97 1140 461 906 907 1545 2229
Fragments arranged by size:
2229 1545 1140 907 906 461 97
PasI CC'CwG_GG
Cuts at: 1348 3491 3743 4393 1348
Size: 2143 252 650 4240
Fragments arranged by size:
4240 2143 650 252
PciI A'CATG_T
Cuts at: 2365 6184 2365
Size: 3819 3466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3638 4133 1108
Size: 2530 495 4260
Fragments arranged by size:
4260 2530 495
PmeI GTTT'AAAC
Cuts at: 4589 4589
Size: 7285
PmlI CAC'GTG
Cuts at: 2406 3715 4337 2406
Size: 1309 622 5354
Fragments arranged by size:
5354 1309 622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5354 5386 6867 6899 5354
Size: 32 1481 32 5740
Fragments arranged by size:
5740 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2689 3708 4398 4482 2155
Size: 534 1019 690 84 4958
Fragments arranged by size:
4958 1019 690 534 84
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7285
PsiI TTA'TAA
Cuts at: 4855 4855
Size: 7285
PstI C_TGCA'G
Cuts at: 830 3292 3523 3681 4239 4438 4637 830
Size: 2462 231 158 558 199 199 3478
Fragments arranged by size:
3478 2462 558 231 199 199 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7285
PvuII CAG'CTG
Cuts at: 3280 3280
Size: 7285
SalI G'TCGA_C
Cuts at: 1936 4627 1936
Size: 2691 4594
SanDI GG'GwC_CC
Cuts at: 3708 3708
Size: 7285
SapI GCTCTTCn'nnn_
Cuts at: 2110 2168 2110
Size: 58 7227
SbfI CC_TGCA'GG
Cuts at: 3292 4637 3292
Size: 1345 5940
ScaI AGT'ACT
Cuts at: 5533 7245 5533
Size: 1712 5573
SexAI A'CCwGG_T
Cuts at: 3472 3472
Size: 7285
SfcI C'TryA_G
Cuts at: 826 1045 2956 3288 3519 3677 4235 4434 4633
Size: 219 1911 332 231 158 558 199 199
Cuts at: 4633 5768 6449 6640 826
Size: 1135 681 191 1471
Fragments arranged by size:
1911 1471 1135 681 558 332 231 219
199 199 191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7285
SmaI CCC'GGG
Cuts at: 1872 4612 1872
Size: 2740 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5389 6290 6552 820
Size: 197 926 3446 901 262 1553
Fragments arranged by size:
3446 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5076 493
Size: 4583 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7285
SphI G_CATG'C
Cuts at: 1132 4643 1132
Size: 3511 3774
SspI AAT'ATT
Cuts at: 5 52 2212 3811 5209 5
Size: 47 2160 1599 1398 2081
Fragments arranged by size:
2160 2081 1599 1398 47
StuI AGG'CCT
Cuts at: 1517 4179 1517
Size: 2662 4623
StyI C'CwwG_G
Cuts at: 513 1065 2004 2110 2359 2791 2881 3024 3976
Size: 552 939 106 249 432 90 143 952
Cuts at: 3976 4168 4473 4724 513
Size: 192 305 251 3074
Fragments arranged by size:
3074 952 939 552 432 305 251 249
192 143 106 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4580 5312 5329 5482 5667 6112 325
Size: 1255 3000 732 17 153 185 445 1498
Fragments arranged by size:
3000 1498 1255 732 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3717 5531 7243
Size: 274 80 33 51 1443 1740 1814 1712
Cuts at: 7243 96
Size: 138
Fragments arranged by size:
1814 1740 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2415 2447 3069 3101 3230 3262 3984 4016 4332
Size: 32 622 32 129 32 722 32 316
Cuts at: 4332 4364 7245 7277 2415
Size: 32 2881 32 2423
Fragments arranged by size:
2881 2423 722 622 316 129 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7285
XbaI T'CTAG_A
Cuts at: 4621 4621
Size: 7285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2806 3994 2806
Size: 1188 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7285
XmaI C'CCGG_G
Cuts at: 1870 4610 1870
Size: 2740 4545
XmnI GAAnn'nnTTC
Cuts at: 2177 5414 2177
Size: 3237 4048
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5093 276
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI KasI
NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacI SacII SfiI SfoI SrfI SwaI