Restriction map for
pFN21AB3774
(Circular) (Six-base) MAPSORT of: pFN21AB3774.seq Check: 6844 from: 1 to: 5755
pFN21AB3774 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2328 3565 278
Size: 53 83 186 936 792 1237 2468
Fragments arranged by size:
2468 1237 936 792 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3042 3098 1937
Size: 1105 56 4594
Fragments arranged by size:
4594 1105 56
Acc65I G'GTAC_C
Cuts at: 2829 3076 2829
Size: 247 5508
AclI AA'CG_TT
Cuts at: 1563 3054 3882 4255 1563
Size: 1491 828 373 3063
Fragments arranged by size:
3063 1491 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3818 5202 5470 1632
Size: 2186 1384 268 1917
Fragments arranged by size:
2186 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2889 4654 5631 1203
Size: 1686 1765 977 1327
Fragments arranged by size:
1765 1686 1327 977
AgeI A'CCGG_T
Cuts at: 2946 4586 2946
Size: 1640 4115
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2381 2413 2381
Size: 32 5723
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaI G_GGCC'C
Cuts at: 2128 2128
Size: 5755
ApaLI G'TGCA_C
Cuts at: 2978 3812 4968 2978
Size: 834 1156 3765
Fragments arranged by size:
3765 1156 834
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2200 2298 2476 3080 1870
Size: 73 257 98 178 604 4545
Fragments arranged by size:
4545 604 257 178 98 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2243 2548 2653 2829
Size: 325 217 627 368 88 305 105 176
Cuts at: 2829 2925 3076 4531 618
Size: 96 151 1455 1842
Fragments arranged by size:
1842 1455 627 368 325 305 217 176
151 105 96 88
BanII G_rGCy'C
Cuts at: 1391 2128 2495 3074 1391
Size: 737 367 579 4072
Fragments arranged by size:
4072 737 579 367
BbeI G_GCGC'C
Cuts at: 2552 2552
Size: 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2975 928
Size: 971 1076 3708
Fragments arranged by size:
3708 1076 971
BbvCI CC'TCA_GC
Cuts at: 2183 2183
Size: 5755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2225 2259 3946 3980 1903
Size: 34 288 34 1687 34 3678
Fragments arranged by size:
3678 1687 288 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2769 5750 2769
Size: 2981 2774
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2160 2523 2658 2982 3816 4972 1790
Size: 338 32 363 135 324 834 1156 2573
Fragments arranged by size:
2573 1156 834 363 338 324 135 32
BmgBI CAC'GTC
Cuts at: 1318 2512 1318
Size: 1194 4561
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2350 2723 2944
Size: 416 53 83 399 24 933 373 221
Cuts at: 2944 3486 4444 442
Size: 542 958 1753
Fragments arranged by size:
1753 958 933 542 416 399 373 221
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 4415 1239
Size: 1169 2007 2579
Fragments arranged by size:
2579 2007 1169
Bpu10I CC'TnA_GC
Cuts at: 2183 2183
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 2549 3562 3944
Size: 53 83 186 936 792 224 1013 382
Cuts at: 3944 4579 275
Size: 635 1451
Fragments arranged by size:
1451 1013 936 792 635 382 224 186
83 53
BsaWI w'CCGG_w
Cuts at: 2320 2946 2964 4188 4586 4860 5007 5418 2320
Size: 626 18 1224 398 274 147 411 2657
Fragments arranged by size:
2657 1224 626 411 398 274 147 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 1178
Size: 30 165 30 978 30 4522
Fragments arranged by size:
4522 978 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5755
BseYI C'CCAG_C
Cuts at: 2779 4958 5454 2779
Size: 2179 496 3080
Fragments arranged by size:
3080 2179 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2015 2982 3816 3901 4972 2015
Size: 967 834 85 1071 2798
Fragments arranged by size:
2798 1071 967 834 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2128 2160 2495 2523 2658 2982
Size: 399 225 113 32 335 28 135 324
Cuts at: 2982 3074 3816 3901 4972 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 324 225 135
113 92 85 32 28
BspEI T'CCGG_A
Cuts at: 2964 5418 2964
Size: 2454 3301
BspHI T'CATG_A
Cuts at: 2532 3644 5374 2532
Size: 1112 1730 2913
Fragments arranged by size:
2913 1730 1112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2493 2634 3110 844
Size: 1649 141 476 3489
Fragments arranged by size:
3489 1649 476 141
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2168 2240 2451 2946 4399 4586 1700
Size: 110 358 72 211 495 1453 187 2869
Fragments arranged by size:
2869 1453 495 358 211 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2311 2709 2769 3085 3452 3837 3854 5306
Size: 339 398 60 316 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 398 385 367 346 339 316
86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2463 2556 2700 2838 513
Size: 552 972 426 93 144 138 3430
Fragments arranged by size:
3430 972 552 426 144 138 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2250 487
Size: 683 56 1024 3992
Fragments arranged by size:
3992 1024 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 3018 3202
Size: 54 1425 50 275 18 267 921 184
Cuts at: 3202 4091 5720 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 921 889 275 267 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 3685 1353
Size: 725 1607 3423
Fragments arranged by size:
3423 1607 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3818 4415 5202 5470 1239
Size: 393 776 1410 597 787 268 1524
Fragments arranged by size:
1524 1410 787 776 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2186
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2124 2125 2437 2665 2124
Size: 1 312 228 5214
Fragments arranged by size:
5214 312 228 1
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2182 2458 2552 2694 4902 1678
Size: 504 276 94 142 2208 2531
Fragments arranged by size:
2531 2208 504 276 142 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2527 2559 2803 2835 4396
Size: 32 135 32 955 32 244 32 1561
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1561 955 244 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2548 2548
Size: 5755
KpnI G_GTAC'C
Cuts at: 2833 3080 2833
Size: 247 5508
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 4869 5053 1305
Size: 969 2595 184 2007
Fragments arranged by size:
2595 2007 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 3020 10
Size: 54 1425 343 267 921 2745
Fragments arranged by size:
2745 1425 921 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 2843 2874 3011 3713 4072 4231
Size: 648 1371 306 31 137 702 359 159
Cuts at: 4231 518
Size: 2042
Fragments arranged by size:
2042 1371 702 648 359 306 159 137
31
MspA1I CmG'CkG
Cuts at: 1606 2226 3171 3848 4996 5241 1606
Size: 620 945 677 1148 245 2120
Fragments arranged by size:
2120 1148 945 677 620 245
NaeI GCC'GGC
Cuts at: 2170 2453 2170
Size: 283 5472
NarI GG'CG_CC
Cuts at: 2549 2549
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2168 2451 2168
Size: 283 5472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2873 3016 3113 4658 1132
Size: 97 1644 143 97 1545 2229
Fragments arranged by size:
2229 1644 1545 143 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2095 1108
Size: 987 4768
PfoI T'CCnGG_A
Cuts at: 2433 2667 2433
Size: 234 5521
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2381 2413 3824 3856 5337 5369 2381
Size: 32 1411 32 1481 32 2767
Fragments arranged by size:
2767 1481 1411 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 2665 2437
Size: 228 5527
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2124 2124
Size: 5755
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 5723
PstI C_TGCA'G
Cuts at: 830 2178 2352 3107 830
Size: 1348 174 755 3478
Fragments arranged by size:
3478 1348 755 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SanDI GG'GwC_CC
Cuts at: 2437 2437
Size: 5755
SapI GCTCTTCn'nnn_
Cuts at: 2078 2078
Size: 5755
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2174 2348 3103 4238 4919 5110 826
Size: 219 1129 174 755 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 755 681 219 191 174
SfoI GGC'GCC
Cuts at: 2550 2550
Size: 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 3021 3194 513
Size: 552 1956 173 3074
Fragments arranged by size:
3074 1956 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2606 3782 3799 3952 4137 4582 325
Size: 1255 1026 1176 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1176 1026 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2310 2342 2663 2695 5715 5747 2310
Size: 32 321 32 3020 32 2318
Fragments arranged by size:
3020 2318 321 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2408 2845 2383
Size: 25 437 5293
Fragments arranged by size:
5293 437 25
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 2396 2738 3884 2396
Size: 342 1146 4267
Fragments arranged by size:
4267 1146 342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2326 3563 276
Size: 53 83 186 936 792 1237 2468
Fragments arranged by size:
2468 1237 936 792 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PvuII RsrII SacI
SacII SexAI SfiI SrfI SwaI