Restriction map for
pFN21AB3769
(Circular) (Six-base) MAPSORT of: pFN21AB3769.seq Check: 1990 from: 1 to: 6622
pFN21AB3769 6622 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3339 3965 1937
Size: 1402 626 4594
Fragments arranged by size:
4594 1402 626
Acc65I G'GTAC_C
Cuts at: 3943 3943
Size: 6622
AclI AA'CG_TT
Cuts at: 1563 3474 4749 5122 1563
Size: 1911 1275 373 3063
Fragments arranged by size:
3063 1911 1275 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4685 6069 6337 1632
Size: 3053 1384 268 1917
Fragments arranged by size:
3053 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 2631 5521 6498 1203
Size: 1428 2890 977 1327
Fragments arranged by size:
2890 1428 1327 977
AgeI A'CCGG_T
Cuts at: 3497 5453 3497
Size: 1956 4666
AhdI GACnn_n'nnGTC
Cuts at: 5351 5351
Size: 6622
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 3058 3184 4365 5937 3058
Size: 126 1181 1572 3743
Fragments arranged by size:
3743 1572 1181 126
ApaI G_GGCC'C
Cuts at: 2778 2778
Size: 6622
ApaLI G'TGCA_C
Cuts at: 2423 4679 5835 2423
Size: 2256 1156 3210
Fragments arranged by size:
3210 2256 1156
ApoI r'AATT_y
Cuts at: 1493 2237 3931 4157 4309 6418 1493
Size: 744 1694 226 152 2109 1697
Fragments arranged by size:
2109 1697 1694 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 3947 1870
Size: 73 2004 4545
Fragments arranged by size:
4545 2004 73
BamHI G'GATC_C
Cuts at: 3952 4319 3952
Size: 367 6255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2492 3128 3943 5398
Size: 325 217 627 255 450 636 815 1455
Cuts at: 5398 618
Size: 1842
Fragments arranged by size:
1842 1455 815 636 627 450 325 255
217
BanII G_rGCy'C
Cuts at: 1391 2508 2535 2778 2840 2928 3810 3941 1391
Size: 1117 27 243 62 88 882 131 4072
Fragments arranged by size:
4072 1117 882 243 131 88 62 27
BbeI G_GCGC'C
Cuts at: 3132 3132
Size: 6622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2059 3681 928
Size: 971 160 1622 3869
Fragments arranged by size:
3869 1622 971 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2845 2879 4813 4847 1903
Size: 34 908 34 1934 34 3678
Fragments arranged by size:
3678 1934 908 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3050 4514 5724 3050
Size: 1464 1210 3948
Fragments arranged by size:
3948 1464 1210
BclI T'GATC_A
Cuts at: 1548 3744 1548
Size: 2196 4426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2160 5233 136
Size: 107 122 71 631 605 488 3073 1525
Fragments arranged by size:
3073 1525 631 605 488 122 107 71
BglII A'GATC_T
Cuts at: 6617 6617
Size: 6622
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2427 2778 4683 5839 1790
Size: 255 382 351 1905 1156 2573
Fragments arranged by size:
2573 1905 1156 382 351 255
BmgBI CAC'GTC
Cuts at: 1318 2632 3199 1318
Size: 1314 567 4741
Fragments arranged by size:
4741 1314 567
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4353 5311 442
Size: 416 53 83 399 24 2936 958 1753
Fragments arranged by size:
2936 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2884 2383
Size: 501 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2975 3659 5282 1239
Size: 1736 684 1623 2579
Fragments arranged by size:
2579 1736 1623 684
Bpu10I CC'TnA_GC
Cuts at: 2834 2834
Size: 6622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4747 5612 5910 4747
Size: 865 298 5459
Fragments arranged by size:
5459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 5285 882
Size: 617 831 2955 2219
Fragments arranged by size:
2955 2219 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 2131 4413 493
Size: 711 927 2282 2702
Fragments arranged by size:
2702 2282 927 711
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2214 2982 3129
Size: 53 83 186 936 633 48 768 147
Cuts at: 3129 3456 4429 4811 5446 275
Size: 327 973 382 635 1451
Fragments arranged by size:
1451 973 936 768 635 633 382 327
186 147 83 53 48
BsaWI w'CCGG_w
Cuts at: 3497 3561 5055 5453 5727 5874 6285 3497
Size: 64 1494 398 274 147 411 3834
Fragments arranged by size:
3834 1494 411 398 274 147 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6397
Fragments arranged by size:
6397 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2756 2936 1186
Size: 1186 384 180 4872
Fragments arranged by size:
4872 1186 384 180
BseYI C'CCAG_C
Cuts at: 2123 3214 3379 5825 6321 2123
Size: 1091 165 2446 496 2424
Fragments arranged by size:
2446 2424 1091 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 3158 3197 3353 3590 1163
Size: 573 540 882 39 156 237 4195
Fragments arranged by size:
4195 882 573 540 237 156 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2682 3616 3760 4072 4833 5861
Size: 275 186 681 934 144 312 761 1028
Cuts at: 5861 1540
Size: 2301
Fragments arranged by size:
2301 1028 934 761 681 312 275 186
144
BsiHKAI G_wGCw'C
Cuts at: 2427 2601 2840 2928 3497 3810 4683 4768 5839
Size: 174 239 88 569 313 873 85 1071
Cuts at: 5839 2427
Size: 3210
Fragments arranged by size:
3210 1071 873 569 313 239 174 88
85
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2427 2508 2535 2601 2778 2840
Size: 399 255 382 81 27 66 177 62
Cuts at: 2840 2928 3497 3810 3941 4683 4768 5839 1391
Size: 88 569 313 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 399 382 313 255
177 131 88 85 81 66 62 27
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 3240 4511 6241 3240
Size: 1271 1730 3621
Fragments arranged by size:
3621 1730 1271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3192 3325 3384 3977 844
Size: 2348 133 59 593 3489
Fragments arranged by size:
3489 2348 593 133 59
BsrBI CCG'CTC
Cuts at: 1394 1823 3583 4509 1394
Size: 429 1760 926 3507
Fragments arranged by size:
3507 1760 926 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5117 5291 65
Size: 1183 3869 174 1396
Fragments arranged by size:
3869 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2681 2959 3497 5266 5453 1700
Size: 110 871 278 538 1769 187 2869
Fragments arranged by size:
2869 1769 871 538 278 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6622
BssHII G'CGCG_C
Cuts at: 1925 2785 2818 2908 1925
Size: 860 33 90 5639
Fragments arranged by size:
5639 860 90 33
BssSI C'ACGA_G
Cuts at: 4682 5694 4682
Size: 1012 5610
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2811 3184 794
Size: 2017 373 4232
Fragments arranged by size:
4232 2017 373
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 3439 1184
Size: 2255 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2131
Size: 6622
BstYI r'GATC_y
Cuts at: 1972 3003 3048 3720 3952 4319 4704 4721 6173
Size: 1031 45 672 232 367 385 17 1452
Cuts at: 6173 6259 6271 6617 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1031 672 385 367 346 232
86 45 17 12
BstZ17I GTA'TAC
Cuts at: 3340 3340
Size: 6622
BtgI C'CryG_G
Cuts at: 513 1065 2014 2370 2535 2676 3132 513
Size: 552 949 356 165 141 456 4003
Fragments arranged by size:
4003 949 552 456 356 165 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2077 2533 487
Size: 683 56 851 456 4576
Fragments arranged by size:
4576 851 683 456 56
BtsI GCAGTG_nn'
Cuts at: 4138 4932 4952 4138
Size: 794 20 5808
Fragments arranged by size:
5808 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6587
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3208 5629 809
Size: 507 1892 2421 1802
Fragments arranged by size:
2421 1892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2276 2544
Size: 54 1425 50 275 18 354 92 268
Cuts at: 2544 2679 3144 3624 4069 4958 6587 8
Size: 135 465 480 445 889 1629 43
Fragments arranged by size:
1629 1425 889 480 465 445 354 275
268 135 92 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2679 4069 1537
Size: 275 867 1390 4090
Fragments arranged by size:
4090 1390 867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4552 1353
Size: 3199 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2753 3593 5209 5583 5729 387
Size: 1064 138 1164 840 1616 374 146 1280
Fragments arranged by size:
1616 1280 1164 1064 840 374 146 138
EcoICRI GAG'CTC
Cuts at: 2838 2926 3808 2838
Size: 88 882 5652
Fragments arranged by size:
5652 882 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2975 3659 4685 5282 6069 6337 1239
Size: 393 1343 684 1026 597 787 268 1524
Fragments arranged by size:
1524 1343 1026 787 684 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2738 2738
Size: 6622
EcoO109I rG'GnC_Cy
Cuts at: 2949 3181 3424 3899 2949
Size: 232 243 475 5672
Fragments arranged by size:
5672 475 243 232
EcoRI G'AATT_C
Cuts at: 3931 4309 3931
Size: 378 6244
EcoRV GAT'ATC
Cuts at: 2364 2364
Size: 6622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6622
FspI TGC'GCA
Cuts at: 2519 2804 5128 2519
Size: 285 2324 4013
Fragments arranged by size:
4013 2324 285
HaeII r_GCGC'y
Cuts at: 1678 2155 2473 2975 3132 5769 1678
Size: 477 318 502 157 2637 2531
Fragments arranged by size:
2637 2531 502 477 318 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2657 2689 3891
Size: 32 135 32 776 32 277 32 1202
Cuts at: 3891 3923 5263 5295 5337 5369 1373
Size: 32 1340 32 42 32 2626
Fragments arranged by size:
2626 1340 1202 776 277 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3966 4212 669
Size: 988 281 2028 246 3079
Fragments arranged by size:
3079 2028 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6622
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KasI G'GCGC_C
Cuts at: 3128 3128
Size: 6622
KpnI G_GTAC'C
Cuts at: 3947 3947
Size: 6622
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2095 2241 5736 5920 1305
Size: 790 146 3495 184 2007
Fragments arranged by size:
3495 2007 790 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 10
Size: 54 1425 343 354 4446
Fragments arranged by size:
4446 1425 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 4580 4939 5098 518
Size: 648 1218 2196 359 159 2042
Fragments arranged by size:
2196 2042 1218 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3134 3230 3870 4038 4715 5863 6108 1606
Size: 1528 96 640 168 677 1148 245 2120
Fragments arranged by size:
2120 1528 1148 677 640 245 168 96
NaeI GCC'GGC
Cuts at: 2683 2683
Size: 6622
NarI GG'CG_CC
Cuts at: 3129 3129
Size: 6622
NcoI C'CATG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 4765
Fragments arranged by size:
4765 1305 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NgoMIV G'CCGG_C
Cuts at: 2681 2681
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NruI TCG'CGA
Cuts at: 3612 3612
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 3980 5525 1132
Size: 97 2751 1545 2229
Fragments arranged by size:
2751 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6622
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6622
PfoI T'CCnGG_A
Cuts at: 3006 3006
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2949 3181 3424 3899 2949
Size: 232 243 475 5672
Fragments arranged by size:
5672 475 243 232
PshAI GACnn'nnGTC
Cuts at: 1613 3534 3732 1613
Size: 1921 198 4503
Fragments arranged by size:
4503 1921 198
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PspOMI G'GGCC_C
Cuts at: 2774 2774
Size: 6622
PstI C_TGCA'G
Cuts at: 830 3066 3974 830
Size: 2236 908 3478
Fragments arranged by size:
3478 2236 908
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 3230 3230
Size: 6622
SacI G_AGCT'C
Cuts at: 2840 2928 3810 2840
Size: 88 882 5652
Fragments arranged by size:
5652 882 88
SacII CC_GC'GG
Cuts at: 3135 3135
Size: 6622
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SanDI GG'GwC_CC
Cuts at: 2949 3424 3899 2949
Size: 475 475 5672
Fragments arranged by size:
5672 475 475
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SexAI A'CCwGG_T
Cuts at: 2178 2178
Size: 6622
SfcI C'TryA_G
Cuts at: 826 1045 3062 3970 5105 5786 5977 826
Size: 219 2017 908 1135 681 191 1471
Fragments arranged by size:
2017 1471 1135 908 681 219 191
SfoI GGC'GCC
Cuts at: 3130 3130
Size: 6622
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3949 1872
Size: 2077 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4726 5627 5889 820
Size: 197 926 2783 901 262 1553
Fragments arranged by size:
2783 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6622
StyI C'CwwG_G
Cuts at: 513 1065 2370 2481 3363 3847 4061 513
Size: 552 1305 111 882 484 214 3074
Fragments arranged by size:
3074 1305 882 552 484 214 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3630 3774 4649 4666 4819 5004
Size: 1255 1125 925 144 875 17 153 185
Cuts at: 5004 5449 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1125 925 875 445 185 153
144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 4868 6580
Size: 274 80 33 51 1443 348 2543 1712
Cuts at: 6580 96
Size: 138
Fragments arranged by size:
2543 1712 1443 348 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6582 6614 6582
Size: 32 6590
Tth111I GACn'n_nGTC
Cuts at: 1316 3617 1316
Size: 2301 4321
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 3634 3854 3913 2179
Size: 1455 220 59 4888
Fragments arranged by size:
4888 1455 220 59
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 3947 1870
Size: 2077 4545
XmnI GAAnn'nnTTC
Cuts at: 4751 4751
Size: 6622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI Bsu36I DraIII FseI FspAI NsiI PacI
PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI